Effect of various growth-promoting factors on preimplantation bovine embryo development in vitro

Effect of various growth-promoting factors on preimplantation bovine embryo development in vitro

One-cell bovine embryos produced by in vitro oocyte maturation (IVM) and in vitro fertilization (IVF) were cultured in chemically defined medium (CDM) to which the following growth-promoting factors were added separately: transferrin (Tf, 10 ug/ml), epidermal growth factor (EGF), transforming growth...

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Veröffentlicht in:Theriogenology 1993-04, Vol.39 (4), p.823-833
Hauptverfasser: Flood, M.R., Gage, T.L., Bunch, T.D.
Format: Artikel
Sprache:eng
Schlagworte:
BOVIN
bovine
CRECIMIENTO
CROISSANCE
CULTIVO DE EMBRIONES
CULTURE D'EMBRYON
defined medium
embryo
GANADO BOVINO
growth factors
HORMONAS
HORMONE
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container_end_page 833
container_issue 4
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container_title Theriogenology
container_volume 39
creator Flood, M.R.
Gage, T.L.
Bunch, T.D.
description One-cell bovine embryos produced by in vitro oocyte maturation (IVM) and in vitro fertilization (IVF) were cultured in chemically defined medium (CDM) to which the following growth-promoting factors were added separately: transferrin (Tf, 10 ug/ml), epidermal growth factor (EGF), transforming growth factor-alpha (TGF-α), transforming growth factor-betal (TGF-β1), insulin-like growth factor-one (IGF-I), insulin-like growth factor-two (IGF-II), platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), and nerve growth factor (NGF), all at 10 ng/ml. The embryos were cultured for a total of 10 days and were assessed. The culture medium was changed every 2 days. There were no beneficial effects of growth factors on embryo development to morula or hatched blastocyst stages of development. However, supplementation with EGF approached significance (P
doi_str_mv 10.1016/0093-691X(93)90421-Z
format Article
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The embryos were cultured for a total of 10 days and were assessed. The culture medium was changed every 2 days. There were no beneficial effects of growth factors on embryo development to morula or hatched blastocyst stages of development. However, supplementation with EGF approached significance (P&lt;0.08) when compared with that of CDM alone at the blastocyst stage of development. 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The embryos were cultured for a total of 10 days and were assessed. The culture medium was changed every 2 days. There were no beneficial effects of growth factors on embryo development to morula or hatched blastocyst stages of development. However, supplementation with EGF approached significance (P&lt;0.08) when compared with that of CDM alone at the blastocyst stage of development. The results suggest that supplementation with growth factors does not improve bovine IVM-IVF embryo development when cultured under chemically-defined conditions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16727256</pmid><doi>10.1016/0093-691X(93)90421-Z</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
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source Elsevier ScienceDirect Journals
subjects BOVIN
bovine
CRECIMIENTO
CROISSANCE
CULTIVO DE EMBRIONES
CULTURE D'EMBRYON
defined medium
embryo
GANADO BOVINO
growth factors
HORMONAS
HORMONE
title Effect of various growth-promoting factors on preimplantation bovine embryo development in vitro
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