Quantitative proteomics by SILAC: practicalities and perspectives for an evolving approach

Mass spectrometry-based quantitative proteomics strategies are ideally adapted to the detection of global protein changes between different biological samples. Among these, SILAC (stable isotope labelling by amino acids in cell culture) has demonstrated a great potential. This method is extremely ac...

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Veröffentlicht in:	M.S. Médecine sciences 2009-10, Vol.25 (10), p.835-842
Hauptverfasser:	Emadali, Anouk, Gallagher-Gambarelli, Maighread
Format:	Artikel
Sprache:	fre
Schlagworte:	Amino Acids - metabolism Animals Cell Culture Techniques - methods Cells, Cultured Humans Isotope Labeling - methods Mass Spectrometry - methods Mice Peptides - analysis Proteins - chemistry Proteins - metabolism Proteomics - methods
Online-Zugang:	Volltext
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