Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti
Regulatory RNA plays a pivotal role in the regulation of bacterial gene expression. Here, five small RNAs were studied in Sinorhizobium meliloti – SmrC15, SmrC16, Sra33, 6S and the signal recognition particle (SRP) RNA, which are conserved among at least seven different Rhizobium and Sinorhizobium s...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2009-12, Vol.390 (2), p.331-336 |
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| creator | Voss, Björn Hölscher, Marion Baumgarth, Birgit Kalbfleisch, Andreas Kaya, Ceren Hess, Wolfgang R. Becker, Anke Evguenieva-Hackenberg, Elena |
| description | Regulatory RNA plays a pivotal role in the regulation of bacterial gene expression. Here, five small RNAs were studied in Sinorhizobium meliloti – SmrC15, SmrC16, Sra33, 6S and the signal recognition particle (SRP) RNA, which are conserved among at least seven different Rhizobium and Sinorhizobium species. The amount of SmrC16 decreased in stationary phase, while the other RNAs were up-regulated. The smallest changes, maximally 2-fold, were observed for 6S RNA. In the distantly related Bradyrhizobium japonicum, the amount of 6S RNA was not increased in stationary phase, suggesting some functional divergence in the roles of this molecule in Rhizobiales in comparison to Escherichia coli. Different decay rates were observed for SmrC15 and SmrC16 of S. meliloti upon rifampicin treatment, revealing posttranscriptional regulation during growth. The use of a Δhfq mutant showed that Hfq protects full-length SmrC16 from degradation and stabilises its specific degradation products. |
| doi_str_mv | 10.1016/j.bbrc.2009.09.125 |
| format | Article |
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Here, five small RNAs were studied in Sinorhizobium meliloti – SmrC15, SmrC16, Sra33, 6S and the signal recognition particle (SRP) RNA, which are conserved among at least seven different Rhizobium and Sinorhizobium species. The amount of SmrC16 decreased in stationary phase, while the other RNAs were up-regulated. The smallest changes, maximally 2-fold, were observed for 6S RNA. In the distantly related Bradyrhizobium japonicum, the amount of 6S RNA was not increased in stationary phase, suggesting some functional divergence in the roles of this molecule in Rhizobiales in comparison to Escherichia coli. Different decay rates were observed for SmrC15 and SmrC16 of S. meliloti upon rifampicin treatment, revealing posttranscriptional regulation during growth. 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Here, five small RNAs were studied in Sinorhizobium meliloti – SmrC15, SmrC16, Sra33, 6S and the signal recognition particle (SRP) RNA, which are conserved among at least seven different Rhizobium and Sinorhizobium species. The amount of SmrC16 decreased in stationary phase, while the other RNAs were up-regulated. The smallest changes, maximally 2-fold, were observed for 6S RNA. In the distantly related Bradyrhizobium japonicum, the amount of 6S RNA was not increased in stationary phase, suggesting some functional divergence in the roles of this molecule in Rhizobiales in comparison to Escherichia coli. Different decay rates were observed for SmrC15 and SmrC16 of S. meliloti upon rifampicin treatment, revealing posttranscriptional regulation during growth. The use of a Δhfq mutant showed that Hfq protects full-length SmrC16 from degradation and stabilises its specific degradation products.</description><subject>6S RNA</subject><subject>Base Sequence</subject><subject>Bradyrhizobium</subject><subject>Bradyrhizobium - genetics</subject><subject>Bradyrhizobium - metabolism</subject><subject>Bradyrhizobium japonicum</subject><subject>Escherichia coli</subject><subject>Hfq</subject><subject>Host Factor 1 Protein - genetics</subject><subject>Host Factor 1 Protein - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Rhizobium</subject><subject>RNA Stability</subject><subject>RNA, Bacterial - biosynthesis</subject><subject>RNA, Bacterial - chemistry</subject><subject>RNA, Bacterial - metabolism</subject><subject>RNA, Untranslated</subject><subject>Sinorhizobium</subject><subject>Sinorhizobium meliloti</subject><subject>Sinorhizobium meliloti - genetics</subject><subject>Sinorhizobium meliloti - metabolism</subject><subject>Small RNA</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1KJDEUhYM4jG07L-BCstJV9dzUXyrgRsQ_aBxwHJhdSFK3NE1VpU2qRN364qbsBnfChUDy3UP4DiGHDBYMWPl7tdDam0UKIBZxWFrskBkDAUnKIN8lMwAok1Sw_3tkP4QVAGN5KX6SPSYqgKosZuT94mXtMQTreuoaGjrVtvTu9ixQ29O7R_vmtFUtBqr6mq69G9AMW1Z90Z-vdgi0xgevavWJRLoeTbzUr_S6eZoC_9re-U3o2NEOW9u6wR6QH41qA_7annPy7_Li_vw6Wf65ujk_WyYmq8oh0ZkGJqCqyyIDKERT8sxkvCoEaOCFUFxlgnPklWEFq3RuVFVitJPDJKLI5uRkkxt_9jRiGGRng8G2VT26MUie5RDFpBDJ42_JlDGeinwC0w1ovAvBYyPX3nbKv0oGcipJruRUkpxKknFiSXHpaJs-6g7rr5VtKxE43QAYbTxb9DIYi73B2vroX9bOfpf_AUanohs</recordid><startdate>20091211</startdate><enddate>20091211</enddate><creator>Voss, Björn</creator><creator>Hölscher, Marion</creator><creator>Baumgarth, Birgit</creator><creator>Kalbfleisch, Andreas</creator><creator>Kaya, Ceren</creator><creator>Hess, Wolfgang R.</creator><creator>Becker, Anke</creator><creator>Evguenieva-Hackenberg, Elena</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20091211</creationdate><title>Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti</title><author>Voss, Björn ; 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Here, five small RNAs were studied in Sinorhizobium meliloti – SmrC15, SmrC16, Sra33, 6S and the signal recognition particle (SRP) RNA, which are conserved among at least seven different Rhizobium and Sinorhizobium species. The amount of SmrC16 decreased in stationary phase, while the other RNAs were up-regulated. The smallest changes, maximally 2-fold, were observed for 6S RNA. In the distantly related Bradyrhizobium japonicum, the amount of 6S RNA was not increased in stationary phase, suggesting some functional divergence in the roles of this molecule in Rhizobiales in comparison to Escherichia coli. Different decay rates were observed for SmrC15 and SmrC16 of S. meliloti upon rifampicin treatment, revealing posttranscriptional regulation during growth. 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| subjects | 6S RNA Base Sequence Bradyrhizobium Bradyrhizobium - genetics Bradyrhizobium - metabolism Bradyrhizobium japonicum Escherichia coli Hfq Host Factor 1 Protein - genetics Host Factor 1 Protein - metabolism Molecular Sequence Data Nucleic Acid Conformation Rhizobium RNA Stability RNA, Bacterial - biosynthesis RNA, Bacterial - chemistry RNA, Bacterial - metabolism RNA, Untranslated Sinorhizobium Sinorhizobium meliloti Sinorhizobium meliloti - genetics Sinorhizobium meliloti - metabolism Small RNA |
| title | Expression of small RNAs in Rhizobiales and protection of a small RNA and its degradation products by Hfq in Sinorhizobium meliloti |
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