Development and validation of an ELISA for quantitation of Bovine Viral Diarrhea Virus antigen in the critical stages of vaccine production

Bovine Viral Diarrhea Virus (BVDV) is the causative agent of a worldwide disease. The virus infects bovines of all ages, causing reproductive problems and contaminating biological products of high commercial value. The large-scale production of BVDV vaccines presents the challenge of processing anti...

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Veröffentlicht in:	Journal of virological methods 2009-12, Vol.162 (1), p.170-178
Hauptverfasser:	Pecora, A., Aguirreburualde, M.S. Perez, Rodriguez, D., Seki, C., Levy, M.S., Bochoeyer, D., Dus Santos, M.J., Wigdorovitz, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Age Animals Antibodies Antigens, Viral - analysis Antigens, Viral - genetics Antigens, Viral - immunology Biological and medical sciences Bovine viral diarrhea virus Bovine Virus Diarrhea-Mucosal Disease - immunology Bovine Virus Diarrhea-Mucosal Disease - prevention & control BVDV Cattle CHO Cells Cricetinae Cricetulus Diarrhea Diarrhea Viruses, Bovine Viral - immunology ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Glycoproteins Immunization Microbiology Quantitation Recombinant Proteins - genetics Recombinant Proteins - immunology Reproducibility of Results Sensitivity and Specificity Techniques used in virology Vaccines Vaccines, Inactivated - immunology Validation Viral Envelope Proteins - analysis Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Viral Vaccines - immunology Virology
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