Imaging of Lipids in Spinal Cord Using Intermediate Pressure Matrix-Assisted Laser Desorption-Linear Ion Trap/Orbitrap MS
A hybrid linear ion trap/Orbitrap mass spectrometer was used to perform tandem mass spectrometry (MS/MS) experiments and high-resolution mass analysis of lipids desorbed from nerve tissue. A dramatic improvement in mass spectral resolution and a decrease in background are observed in the spectra col...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2009-10, Vol.81 (20), p.8488-8495 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8495 |
|---|---|
| container_issue | 20 |
| container_start_page | 8488 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 81 |
| creator | Landgraf, Rachelle R Prieto Conaway, Maria C Garrett, Timothy J Stacpoole, Peter W Yost, Richard A |
| description | A hybrid linear ion trap/Orbitrap mass spectrometer was used to perform tandem mass spectrometry (MS/MS) experiments and high-resolution mass analysis of lipids desorbed from nerve tissue. A dramatic improvement in mass spectral resolution and a decrease in background are observed in the spectra collected from the Orbitrap mass analyzer, which allows generation of more accurate mass spectrometric images of the distribution of lipids within nerve tissue. Employment of both mass analyzers provides a rapid and reliable means of compound identification based on MS/MS fragmentation and high-resolution mass spectrometry (HRMS) accurate mass. |
| doi_str_mv | 10.1021/ac901387u |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_734083483</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>734083483</sourcerecordid><originalsourceid>FETCH-LOGICAL-a406t-1836d2b919d4ec08d9964a32908252f414f4f889d82415b94a0bd47a27101063</originalsourceid><addsrcrecordid>eNpl0U1r3DAQBmBRGppN2kP_QBGFEnpwMpJlWzqG7UcWHBLI9mzGlhwUvJKrsaH59_WSJQvtSQI9zIzeYeyjgEsBUlxhZ0DkuprfsJUoJGSl1vItWwFAnskK4JSdET0BCAGifMdOhakKAYVYsefNDh99eOSx57UfvSXuA38YfcCBr2Oy_BftnzdhcmnnrMfJ8fvkiObk-C1Oyf_Jrok8Tc7yGskl_s1RTOPkY8hqHxwmvomBbxOOV3ep9dNy4bcP79lJjwO5D4fznG1_fN-ub7L67udmfV1nqKCcMqHz0srWCGOV60BbY0qFuTSgZSF7JVSveq2N1VKJojUKobWqQlktf4UyP2cXL2XHFH_PjqZm56lzw4DBxZmaKlegc6XzRX7-Rz7FOS05UCNFZQQouUdfX1CXIlFyfTMmv8P03Aho9stoXpex2E-HgnO7RHeUh_QX8OUAkDoc-oSh8_TqpIRS6tIcHXZ0HOr_hn8BfY6bUw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217910423</pqid></control><display><type>article</type><title>Imaging of Lipids in Spinal Cord Using Intermediate Pressure Matrix-Assisted Laser Desorption-Linear Ion Trap/Orbitrap MS</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Landgraf, Rachelle R ; Prieto Conaway, Maria C ; Garrett, Timothy J ; Stacpoole, Peter W ; Yost, Richard A</creator><creatorcontrib>Landgraf, Rachelle R ; Prieto Conaway, Maria C ; Garrett, Timothy J ; Stacpoole, Peter W ; Yost, Richard A</creatorcontrib><description>A hybrid linear ion trap/Orbitrap mass spectrometer was used to perform tandem mass spectrometry (MS/MS) experiments and high-resolution mass analysis of lipids desorbed from nerve tissue. A dramatic improvement in mass spectral resolution and a decrease in background are observed in the spectra collected from the Orbitrap mass analyzer, which allows generation of more accurate mass spectrometric images of the distribution of lipids within nerve tissue. Employment of both mass analyzers provides a rapid and reliable means of compound identification based on MS/MS fragmentation and high-resolution mass spectrometry (HRMS) accurate mass.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac901387u</identifier><identifier>PMID: 19751051</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical chemistry ; Animals ; Chemical compounds ; Chemistry ; Exact sciences and technology ; Female ; Lasers ; Lipid Metabolism ; Lipids ; Lipids - analysis ; Mass spectrometry ; Molecular Imaging - methods ; Phospholipids - analysis ; Phospholipids - metabolism ; Pressure ; Rats ; Rats, Sprague-Dawley ; Spectrometric and optical methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Spinal Cord - metabolism ; Tissues</subject><ispartof>Analytical chemistry (Washington), 2009-10, Vol.81 (20), p.8488-8495</ispartof><rights>Copyright © 2009 American Chemical Society</rights><rights>2009 INIST-CNRS</rights><rights>Copyright American Chemical Society Oct 15, 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a406t-1836d2b919d4ec08d9964a32908252f414f4f889d82415b94a0bd47a27101063</citedby><cites>FETCH-LOGICAL-a406t-1836d2b919d4ec08d9964a32908252f414f4f889d82415b94a0bd47a27101063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac901387u$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac901387u$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22062869$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19751051$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Landgraf, Rachelle R</creatorcontrib><creatorcontrib>Prieto Conaway, Maria C</creatorcontrib><creatorcontrib>Garrett, Timothy J</creatorcontrib><creatorcontrib>Stacpoole, Peter W</creatorcontrib><creatorcontrib>Yost, Richard A</creatorcontrib><title>Imaging of Lipids in Spinal Cord Using Intermediate Pressure Matrix-Assisted Laser Desorption-Linear Ion Trap/Orbitrap MS</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A hybrid linear ion trap/Orbitrap mass spectrometer was used to perform tandem mass spectrometry (MS/MS) experiments and high-resolution mass analysis of lipids desorbed from nerve tissue. A dramatic improvement in mass spectral resolution and a decrease in background are observed in the spectra collected from the Orbitrap mass analyzer, which allows generation of more accurate mass spectrometric images of the distribution of lipids within nerve tissue. Employment of both mass analyzers provides a rapid and reliable means of compound identification based on MS/MS fragmentation and high-resolution mass spectrometry (HRMS) accurate mass.</description><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Chemical compounds</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Female</subject><subject>Lasers</subject><subject>Lipid Metabolism</subject><subject>Lipids</subject><subject>Lipids - analysis</subject><subject>Mass spectrometry</subject><subject>Molecular Imaging - methods</subject><subject>Phospholipids - analysis</subject><subject>Phospholipids - metabolism</subject><subject>Pressure</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Spinal Cord - metabolism</subject><subject>Tissues</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0U1r3DAQBmBRGppN2kP_QBGFEnpwMpJlWzqG7UcWHBLI9mzGlhwUvJKrsaH59_WSJQvtSQI9zIzeYeyjgEsBUlxhZ0DkuprfsJUoJGSl1vItWwFAnskK4JSdET0BCAGifMdOhakKAYVYsefNDh99eOSx57UfvSXuA38YfcCBr2Oy_BftnzdhcmnnrMfJ8fvkiObk-C1Oyf_Jrok8Tc7yGskl_s1RTOPkY8hqHxwmvomBbxOOV3ep9dNy4bcP79lJjwO5D4fznG1_fN-ub7L67udmfV1nqKCcMqHz0srWCGOV60BbY0qFuTSgZSF7JVSveq2N1VKJojUKobWqQlktf4UyP2cXL2XHFH_PjqZm56lzw4DBxZmaKlegc6XzRX7-Rz7FOS05UCNFZQQouUdfX1CXIlFyfTMmv8P03Aho9stoXpex2E-HgnO7RHeUh_QX8OUAkDoc-oSh8_TqpIRS6tIcHXZ0HOr_hn8BfY6bUw</recordid><startdate>20091015</startdate><enddate>20091015</enddate><creator>Landgraf, Rachelle R</creator><creator>Prieto Conaway, Maria C</creator><creator>Garrett, Timothy J</creator><creator>Stacpoole, Peter W</creator><creator>Yost, Richard A</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20091015</creationdate><title>Imaging of Lipids in Spinal Cord Using Intermediate Pressure Matrix-Assisted Laser Desorption-Linear Ion Trap/Orbitrap MS</title><author>Landgraf, Rachelle R ; Prieto Conaway, Maria C ; Garrett, Timothy J ; Stacpoole, Peter W ; Yost, Richard A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a406t-1836d2b919d4ec08d9964a32908252f414f4f889d82415b94a0bd47a27101063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Chemical compounds</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Female</topic><topic>Lasers</topic><topic>Lipid Metabolism</topic><topic>Lipids</topic><topic>Lipids - analysis</topic><topic>Mass spectrometry</topic><topic>Molecular Imaging - methods</topic><topic>Phospholipids - analysis</topic><topic>Phospholipids - metabolism</topic><topic>Pressure</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Spinal Cord - metabolism</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Landgraf, Rachelle R</creatorcontrib><creatorcontrib>Prieto Conaway, Maria C</creatorcontrib><creatorcontrib>Garrett, Timothy J</creatorcontrib><creatorcontrib>Stacpoole, Peter W</creatorcontrib><creatorcontrib>Yost, Richard A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Landgraf, Rachelle R</au><au>Prieto Conaway, Maria C</au><au>Garrett, Timothy J</au><au>Stacpoole, Peter W</au><au>Yost, Richard A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Imaging of Lipids in Spinal Cord Using Intermediate Pressure Matrix-Assisted Laser Desorption-Linear Ion Trap/Orbitrap MS</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2009-10-15</date><risdate>2009</risdate><volume>81</volume><issue>20</issue><spage>8488</spage><epage>8495</epage><pages>8488-8495</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A hybrid linear ion trap/Orbitrap mass spectrometer was used to perform tandem mass spectrometry (MS/MS) experiments and high-resolution mass analysis of lipids desorbed from nerve tissue. A dramatic improvement in mass spectral resolution and a decrease in background are observed in the spectra collected from the Orbitrap mass analyzer, which allows generation of more accurate mass spectrometric images of the distribution of lipids within nerve tissue. Employment of both mass analyzers provides a rapid and reliable means of compound identification based on MS/MS fragmentation and high-resolution mass spectrometry (HRMS) accurate mass.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>19751051</pmid><doi>10.1021/ac901387u</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2009-10, Vol.81 (20), p.8488-8495 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_734083483 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Analytical chemistry Animals Chemical compounds Chemistry Exact sciences and technology Female Lasers Lipid Metabolism Lipids Lipids - analysis Mass spectrometry Molecular Imaging - methods Phospholipids - analysis Phospholipids - metabolism Pressure Rats Rats, Sprague-Dawley Spectrometric and optical methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spinal Cord - metabolism Tissues |
| title | Imaging of Lipids in Spinal Cord Using Intermediate Pressure Matrix-Assisted Laser Desorption-Linear Ion Trap/Orbitrap MS |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T17%3A26%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Imaging%20of%20Lipids%20in%20Spinal%20Cord%20Using%20Intermediate%20Pressure%20Matrix-Assisted%20Laser%20Desorption-Linear%20Ion%20Trap/Orbitrap%20MS&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Landgraf,%20Rachelle%20R&rft.date=2009-10-15&rft.volume=81&rft.issue=20&rft.spage=8488&rft.epage=8495&rft.pages=8488-8495&rft.issn=0003-2700&rft.eissn=1520-6882&rft.coden=ANCHAM&rft_id=info:doi/10.1021/ac901387u&rft_dat=%3Cproquest_cross%3E734083483%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=217910423&rft_id=info:pmid/19751051&rfr_iscdi=true |