Simultaneous and Label-Free Determination of Wild-Type and Mutant p53 at a Single Surface Plasmon Resonance Chip Preimmobilized with Consensus DNA and Monoclonal Antibody

Simultaneous determination of wild-type and total p53 proteins (wild-type and mutant combined) present in cancer cell lysates has been performed with a dual-channel surface plasmon resonance (SPR) instrument. To achieve specificity, each channel of the SPR chip was modified with a consensus double-s...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical chemistry (Washington) 2009-10, Vol.81 (20), p.8441-8446
Hauptverfasser:	Wang, Yongcan, Zhu, Xu, Wu, Minghua, Xia, Ning, Wang, Jianxiu, Zhou, Feimeng
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Analytical, structural and metabolic biochemistry Antibodies, Immobilized - chemistry Antibodies, Immobilized - immunology Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Antibody Specificity Base Sequence Biological and medical sciences Cancer Cell Line, Tumor Cells Chemistry Consensus Sequence Dextrans - chemistry DNA - chemistry DNA - genetics Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Humans Immunoassay Miscellaneous Monoclonal antibodies Mutant Proteins - analysis Mutant Proteins - genetics Mutant Proteins - immunology Mutation Proteins Signal transduction Staining and Labeling Surface Plasmon Resonance - instrumentation Surface Plasmon Resonance - methods Time Factors Tumor Suppressor Protein p53 - analysis Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - immunology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!