In vivo induction of the autophagic machinery in human bone marrow cells during Leishmania donovani complex infection
Abstract Autophagy is a homeostatic process promoting cell survival in periods of stress. The induction of the autophagic machinery has also been implicated in both innate and adaptive immunity. Leishmania donovani , which is the causative pathogen of visceral leishmaniasis, is an intracellular para...
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| Veröffentlicht in: | Parasitology international 2009-12, Vol.58 (4), p.475-477 |
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| creator | Mitroulis, Ioannis Kourtzelis, Ioannis Papadopoulos, Vassileios P Mimidis, Konstantinos Speletas, Matthaios Ritis, Konstantinos |
| description | Abstract Autophagy is a homeostatic process promoting cell survival in periods of stress. The induction of the autophagic machinery has also been implicated in both innate and adaptive immunity. Leishmania donovani , which is the causative pathogen of visceral leishmaniasis, is an intracellular parasite that invades and multiplies in bone marrow macrophages. We describe the induction of host cell autophagic machinery during acute natural bone marrow infection by L. donovani complex, detected by LC3B immunoblot. The successful treatment with liposomal amphotericin B resulted in the resolution of this phenomenon. Even though the role of autophagy in parasite biology has been previously studied, our findings show for the first time the in vivο host cell LC3B conversion as a marker of the induction of the autophagic machinery during infection with Leishmania parasite in real time conditions. |
| doi_str_mv | 10.1016/j.parint.2009.07.002 |
| format | Article |
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The induction of the autophagic machinery has also been implicated in both innate and adaptive immunity. Leishmania donovani , which is the causative pathogen of visceral leishmaniasis, is an intracellular parasite that invades and multiplies in bone marrow macrophages. We describe the induction of host cell autophagic machinery during acute natural bone marrow infection by L. donovani complex, detected by LC3B immunoblot. The successful treatment with liposomal amphotericin B resulted in the resolution of this phenomenon. Even though the role of autophagy in parasite biology has been previously studied, our findings show for the first time the in vivο host cell LC3B conversion as a marker of the induction of the autophagic machinery during infection with Leishmania parasite in real time conditions.</description><identifier>ISSN: 1383-5769</identifier><identifier>EISSN: 1873-0329</identifier><identifier>DOI: 10.1016/j.parint.2009.07.002</identifier><identifier>PMID: 19591960</identifier><language>eng</language><publisher>Netherlands: Elsevier Ireland Ltd</publisher><subject>Adaptor Proteins, Signal Transducing - metabolism ; Amphotericin B - therapeutic use ; Animals ; Antiprotozoal Agents - therapeutic use ; Autophagy ; Autophagy-Related Protein 8 Family ; Bone Marrow Cells - parasitology ; Bone Marrow Cells - physiology ; Bone marrow macrophages ; Gastroenterology and Hepatology ; Host-Pathogen Interactions ; Humans ; Infectious Disease ; LC3B ; Leishmania donovani ; Leishmania donovani - drug effects ; Leishmania donovani - pathogenicity ; Leishmaniasis, Visceral - drug therapy ; Leishmaniasis, Visceral - parasitology ; Macrophages - parasitology ; Macrophages - physiology ; Male ; Microfilament Proteins - metabolism ; Middle Aged</subject><ispartof>Parasitology international, 2009-12, Vol.58 (4), p.475-477</ispartof><rights>Elsevier Ireland Ltd</rights><rights>2009 Elsevier Ireland Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-f39d99315b928c182d2854c0776e08f786eeccdb9c8b8a8541b01b591ab8e9093</citedby><cites>FETCH-LOGICAL-c542t-f39d99315b928c182d2854c0776e08f786eeccdb9c8b8a8541b01b591ab8e9093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.parint.2009.07.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19591960$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitroulis, Ioannis</creatorcontrib><creatorcontrib>Kourtzelis, Ioannis</creatorcontrib><creatorcontrib>Papadopoulos, Vassileios P</creatorcontrib><creatorcontrib>Mimidis, Konstantinos</creatorcontrib><creatorcontrib>Speletas, Matthaios</creatorcontrib><creatorcontrib>Ritis, Konstantinos</creatorcontrib><title>In vivo induction of the autophagic machinery in human bone marrow cells during Leishmania donovani complex infection</title><title>Parasitology international</title><addtitle>Parasitol Int</addtitle><description>Abstract Autophagy is a homeostatic process promoting cell survival in periods of stress. The induction of the autophagic machinery has also been implicated in both innate and adaptive immunity. Leishmania donovani , which is the causative pathogen of visceral leishmaniasis, is an intracellular parasite that invades and multiplies in bone marrow macrophages. We describe the induction of host cell autophagic machinery during acute natural bone marrow infection by L. donovani complex, detected by LC3B immunoblot. The successful treatment with liposomal amphotericin B resulted in the resolution of this phenomenon. Even though the role of autophagy in parasite biology has been previously studied, our findings show for the first time the in vivο host cell LC3B conversion as a marker of the induction of the autophagic machinery during infection with Leishmania parasite in real time conditions.</description><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>Amphotericin B - therapeutic use</subject><subject>Animals</subject><subject>Antiprotozoal Agents - therapeutic use</subject><subject>Autophagy</subject><subject>Autophagy-Related Protein 8 Family</subject><subject>Bone Marrow Cells - parasitology</subject><subject>Bone Marrow Cells - physiology</subject><subject>Bone marrow macrophages</subject><subject>Gastroenterology and Hepatology</subject><subject>Host-Pathogen Interactions</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>LC3B</subject><subject>Leishmania donovani</subject><subject>Leishmania donovani - drug effects</subject><subject>Leishmania donovani - pathogenicity</subject><subject>Leishmaniasis, Visceral - drug therapy</subject><subject>Leishmaniasis, Visceral - parasitology</subject><subject>Macrophages - parasitology</subject><subject>Macrophages - physiology</subject><subject>Male</subject><subject>Microfilament Proteins - metabolism</subject><subject>Middle Aged</subject><issn>1383-5769</issn><issn>1873-0329</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk2v1CAUhhuj8V6v_gNjWOmq9QBtgY2JufHjJpO4UNeE0tNbxhZGaEfn30udSUxc6IoTeM4H73uK4jmFigJtX--rg4nOLxUDUBWICoA9KK6pFLwEztTDHHPJy0a06qp4ktIegDZC0MfFFVWNoqqF62K98-TojoE43692ccGTMJBlRGLWJRxGc-8smY0dncd4yhQZ19l40gWP-T7G8INYnKZE-jVPc0926NKYCWdIH3w45ojYMB8m_JmzB_zd42nxaDBTwmeX86b4-v7dl9uP5e7Th7vbt7vSNjVbyoGrXilOm04xaalkPZNNbUGIFkEOQraI1vadsrKTJj_RDmiXv2Y6iQoUvyleneseYvi-Ylr07NI2rvEY1qQFr0EyoepMvvwnySjllNU8g_UZtDGkFHHQh-iyECdNQW_G6L0-G6M3YzQInY3JaS8u9dduxv5P0sWJDLw5A5j1ODqMOlmH3mLvYhZN98H9r8PfBezkvLNm-oYnTPuwRp-11lQnpkF_3pZj2w1QACBqxX8BZ3W3Tw</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Mitroulis, Ioannis</creator><creator>Kourtzelis, Ioannis</creator><creator>Papadopoulos, Vassileios P</creator><creator>Mimidis, Konstantinos</creator><creator>Speletas, Matthaios</creator><creator>Ritis, Konstantinos</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>C1K</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20091201</creationdate><title>In vivo induction of the autophagic machinery in human bone marrow cells during Leishmania donovani complex infection</title><author>Mitroulis, Ioannis ; Kourtzelis, Ioannis ; Papadopoulos, Vassileios P ; Mimidis, Konstantinos ; Speletas, Matthaios ; Ritis, Konstantinos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-f39d99315b928c182d2854c0776e08f786eeccdb9c8b8a8541b01b591ab8e9093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adaptor Proteins, Signal Transducing - metabolism</topic><topic>Amphotericin B - therapeutic use</topic><topic>Animals</topic><topic>Antiprotozoal Agents - therapeutic use</topic><topic>Autophagy</topic><topic>Autophagy-Related Protein 8 Family</topic><topic>Bone Marrow Cells - parasitology</topic><topic>Bone Marrow Cells - physiology</topic><topic>Bone marrow macrophages</topic><topic>Gastroenterology and Hepatology</topic><topic>Host-Pathogen Interactions</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>LC3B</topic><topic>Leishmania donovani</topic><topic>Leishmania donovani - drug effects</topic><topic>Leishmania donovani - pathogenicity</topic><topic>Leishmaniasis, Visceral - drug therapy</topic><topic>Leishmaniasis, Visceral - parasitology</topic><topic>Macrophages - parasitology</topic><topic>Macrophages - physiology</topic><topic>Male</topic><topic>Microfilament Proteins - metabolism</topic><topic>Middle Aged</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitroulis, Ioannis</creatorcontrib><creatorcontrib>Kourtzelis, Ioannis</creatorcontrib><creatorcontrib>Papadopoulos, Vassileios P</creatorcontrib><creatorcontrib>Mimidis, Konstantinos</creatorcontrib><creatorcontrib>Speletas, Matthaios</creatorcontrib><creatorcontrib>Ritis, Konstantinos</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitroulis, Ioannis</au><au>Kourtzelis, Ioannis</au><au>Papadopoulos, Vassileios P</au><au>Mimidis, Konstantinos</au><au>Speletas, Matthaios</au><au>Ritis, Konstantinos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo induction of the autophagic machinery in human bone marrow cells during Leishmania donovani complex infection</atitle><jtitle>Parasitology international</jtitle><addtitle>Parasitol Int</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>58</volume><issue>4</issue><spage>475</spage><epage>477</epage><pages>475-477</pages><issn>1383-5769</issn><eissn>1873-0329</eissn><abstract>Abstract Autophagy is a homeostatic process promoting cell survival in periods of stress. The induction of the autophagic machinery has also been implicated in both innate and adaptive immunity. Leishmania donovani , which is the causative pathogen of visceral leishmaniasis, is an intracellular parasite that invades and multiplies in bone marrow macrophages. We describe the induction of host cell autophagic machinery during acute natural bone marrow infection by L. donovani complex, detected by LC3B immunoblot. The successful treatment with liposomal amphotericin B resulted in the resolution of this phenomenon. Even though the role of autophagy in parasite biology has been previously studied, our findings show for the first time the in vivο host cell LC3B conversion as a marker of the induction of the autophagic machinery during infection with Leishmania parasite in real time conditions.</abstract><cop>Netherlands</cop><pub>Elsevier Ireland Ltd</pub><pmid>19591960</pmid><doi>10.1016/j.parint.2009.07.002</doi><tpages>3</tpages></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - metabolism Amphotericin B - therapeutic use Animals Antiprotozoal Agents - therapeutic use Autophagy Autophagy-Related Protein 8 Family Bone Marrow Cells - parasitology Bone Marrow Cells - physiology Bone marrow macrophages Gastroenterology and Hepatology Host-Pathogen Interactions Humans Infectious Disease LC3B Leishmania donovani Leishmania donovani - drug effects Leishmania donovani - pathogenicity Leishmaniasis, Visceral - drug therapy Leishmaniasis, Visceral - parasitology Macrophages - parasitology Macrophages - physiology Male Microfilament Proteins - metabolism Middle Aged |
| title | In vivo induction of the autophagic machinery in human bone marrow cells during Leishmania donovani complex infection |
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