Facile and Controllable Loading of Single-Stranded DNA on Gold Nanoparticles
A facile strategy of loading thiolated single-stranded DNA (ss-DNA) on gold nanoparticles (NPs) has been developed. The gold NPs stabilized by nonionic fluorosurfactant (i.e., Zonyl FSN) are simply mixed with the thiolated ssDNA in the presence of NaCl (up to 1.0 M), and the ssDNA−NP conjugates are...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2009-10, Vol.81 (20), p.8523-8528 |
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| creator | Zu, Yanbing Gao, Zhiqiang |
| description | A facile strategy of loading thiolated single-stranded DNA (ss-DNA) on gold nanoparticles (NPs) has been developed. The gold NPs stabilized by nonionic fluorosurfactant (i.e., Zonyl FSN) are simply mixed with the thiolated ssDNA in the presence of NaCl (up to 1.0 M), and the ssDNA−NP conjugates are attained after an incubation of 2 h. The loading density of the ssDNA is controlled by the salt concentration in the immobilization solution. The FSN capping layer inhibits effectively the nonspecific adsorption of nucleobases on the NPs but allows for rapid attachment of the thiolated ssDNA through the sulfur−gold linkage, which may lead to an upright orientation of the immobilized ssDNA. The maximum loading density of the ssDNA obtained in 1.0 M NaCl is sensitive to several factors, such as the type of the spacer, the length of the ssDNA strands, and the NP size. The hybridization behavior of the ssDNA−NP conjugates with complementary ssDNA in solution was examined. For the conjugates of 13 nm-diameter NPs, the hybridization efficiency can reach ∼60% when the surface density of the ssDNA on the gold NP is lower than ∼15 pmol/cm2. As the ssDNA probes are more densely packed on the NPs, the hybridization efficiency drops consequently. |
| doi_str_mv | 10.1021/ac901459v |
| format | Article |
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The gold NPs stabilized by nonionic fluorosurfactant (i.e., Zonyl FSN) are simply mixed with the thiolated ssDNA in the presence of NaCl (up to 1.0 M), and the ssDNA−NP conjugates are attained after an incubation of 2 h. The loading density of the ssDNA is controlled by the salt concentration in the immobilization solution. The FSN capping layer inhibits effectively the nonspecific adsorption of nucleobases on the NPs but allows for rapid attachment of the thiolated ssDNA through the sulfur−gold linkage, which may lead to an upright orientation of the immobilized ssDNA. The maximum loading density of the ssDNA obtained in 1.0 M NaCl is sensitive to several factors, such as the type of the spacer, the length of the ssDNA strands, and the NP size. The hybridization behavior of the ssDNA−NP conjugates with complementary ssDNA in solution was examined. For the conjugates of 13 nm-diameter NPs, the hybridization efficiency can reach ∼60% when the surface density of the ssDNA on the gold NP is lower than ∼15 pmol/cm2. As the ssDNA probes are more densely packed on the NPs, the hybridization efficiency drops consequently.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac901459v</identifier><identifier>PMID: 19751052</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Adsorption ; Analytical, structural and metabolic biochemistry ; Base Sequence ; Biological and medical sciences ; Deoxyribonucleic acid ; DNA ; Dna, deoxyribonucleoproteins ; DNA, Single-Stranded - chemistry ; DNA, Single-Stranded - genetics ; Fundamental and applied biological sciences. 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Chem</addtitle><description>A facile strategy of loading thiolated single-stranded DNA (ss-DNA) on gold nanoparticles (NPs) has been developed. The gold NPs stabilized by nonionic fluorosurfactant (i.e., Zonyl FSN) are simply mixed with the thiolated ssDNA in the presence of NaCl (up to 1.0 M), and the ssDNA−NP conjugates are attained after an incubation of 2 h. The loading density of the ssDNA is controlled by the salt concentration in the immobilization solution. The FSN capping layer inhibits effectively the nonspecific adsorption of nucleobases on the NPs but allows for rapid attachment of the thiolated ssDNA through the sulfur−gold linkage, which may lead to an upright orientation of the immobilized ssDNA. The maximum loading density of the ssDNA obtained in 1.0 M NaCl is sensitive to several factors, such as the type of the spacer, the length of the ssDNA strands, and the NP size. The hybridization behavior of the ssDNA−NP conjugates with complementary ssDNA in solution was examined. For the conjugates of 13 nm-diameter NPs, the hybridization efficiency can reach ∼60% when the surface density of the ssDNA on the gold NP is lower than ∼15 pmol/cm2. As the ssDNA probes are more densely packed on the NPs, the hybridization efficiency drops consequently.</description><subject>Adsorption</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>DNA, Single-Stranded - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Hybridization</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanoparticles</subject><subject>Nucleic Acid Hybridization</subject><subject>Nucleic acids</subject><subject>Organic Chemicals - chemistry</subject><subject>Sulfur - chemistry</subject><subject>Surface Properties</subject><subject>Surface-Active Agents - chemistry</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0EFLwzAUB_AgipvTg19AiiDiofqSpk17HNNNoczDdi-vaSIdXTOTVvDbG1nZQC95EH689-dPyDWFRwqMPqHMgPI4-zohYxozCJM0ZadkDABRyATAiFw4twGgFGhyTkY0EzGFmI1JPkdZNyrAtgpmpu2saRos_UdusKrbj8DoYOVno8JVZ71SVfC8nAamDRamqYIltmaHtqtlo9wlOdPYOHU1zAlZz1_Ws9cwf1-8zaZ5iDwSXahYmXGdCJVE_oEorhhVUpVxiXGp0gqTTAgNacp9Tk8jjVhpijIWQmASTcj9fu3Oms9eua7Y1k4qH7xVpneFiDikDITw8vaP3Jjetj5bwahIRUY59-hhj6Q1zlmli52tt2i_CwrFb7_FoV9vb4aFfblV1VEOhXpwNwB0EhvtK5O1OzjGIGGp4EeH0h1D_T_4A48zjHQ</recordid><startdate>20091015</startdate><enddate>20091015</enddate><creator>Zu, Yanbing</creator><creator>Gao, Zhiqiang</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20091015</creationdate><title>Facile and Controllable Loading of Single-Stranded DNA on Gold Nanoparticles</title><author>Zu, Yanbing ; Gao, Zhiqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a437t-e2b94f67e6367e035d21eceb5ba5be8da6977f0884197b943faadf1ac5777a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adsorption</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>DNA, Single-Stranded - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Hybridization</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Nanoparticles</topic><topic>Nucleic Acid Hybridization</topic><topic>Nucleic acids</topic><topic>Organic Chemicals - chemistry</topic><topic>Sulfur - chemistry</topic><topic>Surface Properties</topic><topic>Surface-Active Agents - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zu, Yanbing</creatorcontrib><creatorcontrib>Gao, Zhiqiang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zu, Yanbing</au><au>Gao, Zhiqiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Facile and Controllable Loading of Single-Stranded DNA on Gold Nanoparticles</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2009-10-15</date><risdate>2009</risdate><volume>81</volume><issue>20</issue><spage>8523</spage><epage>8528</epage><pages>8523-8528</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A facile strategy of loading thiolated single-stranded DNA (ss-DNA) on gold nanoparticles (NPs) has been developed. The gold NPs stabilized by nonionic fluorosurfactant (i.e., Zonyl FSN) are simply mixed with the thiolated ssDNA in the presence of NaCl (up to 1.0 M), and the ssDNA−NP conjugates are attained after an incubation of 2 h. The loading density of the ssDNA is controlled by the salt concentration in the immobilization solution. The FSN capping layer inhibits effectively the nonspecific adsorption of nucleobases on the NPs but allows for rapid attachment of the thiolated ssDNA through the sulfur−gold linkage, which may lead to an upright orientation of the immobilized ssDNA. The maximum loading density of the ssDNA obtained in 1.0 M NaCl is sensitive to several factors, such as the type of the spacer, the length of the ssDNA strands, and the NP size. The hybridization behavior of the ssDNA−NP conjugates with complementary ssDNA in solution was examined. For the conjugates of 13 nm-diameter NPs, the hybridization efficiency can reach ∼60% when the surface density of the ssDNA on the gold NP is lower than ∼15 pmol/cm2. As the ssDNA probes are more densely packed on the NPs, the hybridization efficiency drops consequently.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>19751052</pmid><doi>10.1021/ac901459v</doi><tpages>6</tpages></addata></record> |
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| subjects | Adsorption Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Deoxyribonucleic acid DNA Dna, deoxyribonucleoproteins DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics Fundamental and applied biological sciences. Psychology Gold Gold - chemistry Hybridization Metal Nanoparticles - chemistry Nanoparticles Nucleic Acid Hybridization Nucleic acids Organic Chemicals - chemistry Sulfur - chemistry Surface Properties Surface-Active Agents - chemistry |
| title | Facile and Controllable Loading of Single-Stranded DNA on Gold Nanoparticles |
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