Laboratory investigation and phylogenetic analysis of enteroviruses involved in an aseptic meningitis outbreak in Greece during the summer of 2007
Abstract Background Aseptic meningitis is the most commonly observed CNS infection and is mainly attributed to Non-Polio Enteroviruses (EV). Objective Identification and genetic analysis of the EV involved in the recent aseptic meningitis outbreak which occurred in Greece, during the summer of 2007....
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| Veröffentlicht in: | J Clin Virol 2009-11, Vol.46 (3), p.270-274 |
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| creator | Logotheti, Maria Pogka, Vasiliki Horefti, Elina Papadakos, Konstantinos Giannaki, Maria Pangalis, Anastasia Sgouras, Dionyssios Mentis, Andreas |
| description | Abstract Background Aseptic meningitis is the most commonly observed CNS infection and is mainly attributed to Non-Polio Enteroviruses (EV). Objective Identification and genetic analysis of the EV involved in the recent aseptic meningitis outbreak which occurred in Greece, during the summer of 2007. Study design In total, 213 CSF and faecal samples were examined for EV presence by culture, while enteroviral RNA detection was performed by nucleic acid sequence-based amplification assay (NASBA). EV strains were typed by seroneutralization, as well as nested RT-PCR followed by VP1-2A gene partial sequencing. Phylogenetic analysis was carried out for the identification of the genetic relatedness among the isolated EV strains. Results EV detection rate in CSF and faecal samples was 43.9% and 70.8%, respectively. EV serotyping and VP1 region analysis revealed the predominance of echovirus 4 (ECV4) serotype and the circulation of ECV6, 9, 14, 25, Coxsackie A6, A15, A24 and Coxsackie B1 serotypes. All ECV4 isolates presented a 98.7% similarity in nucleotide sequence, with a Spanish ECV4 strain, isolated during a meningitis outbreak in 2006. Conclusions It is the first time that ECV4 is associated with an aseptic meningitis outbreak in Greece, during which 9 different EV serotypes were co-circulating. All Greek ECV4 isolates were closely related to the Spanish ECV4 strain. Genetic analysis of the VP1 gene can significantly contribute to the revelation of the endemic EV strains circulation pattern and their phylogenetic relationship with enteroviruses involved in epidemics of distant geographical areas at different time periods. |
| doi_str_mv | 10.1016/j.jcv.2009.07.019 |
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Objective Identification and genetic analysis of the EV involved in the recent aseptic meningitis outbreak which occurred in Greece, during the summer of 2007. Study design In total, 213 CSF and faecal samples were examined for EV presence by culture, while enteroviral RNA detection was performed by nucleic acid sequence-based amplification assay (NASBA). EV strains were typed by seroneutralization, as well as nested RT-PCR followed by VP1-2A gene partial sequencing. Phylogenetic analysis was carried out for the identification of the genetic relatedness among the isolated EV strains. Results EV detection rate in CSF and faecal samples was 43.9% and 70.8%, respectively. EV serotyping and VP1 region analysis revealed the predominance of echovirus 4 (ECV4) serotype and the circulation of ECV6, 9, 14, 25, Coxsackie A6, A15, A24 and Coxsackie B1 serotypes. All ECV4 isolates presented a 98.7% similarity in nucleotide sequence, with a Spanish ECV4 strain, isolated during a meningitis outbreak in 2006. Conclusions It is the first time that ECV4 is associated with an aseptic meningitis outbreak in Greece, during which 9 different EV serotypes were co-circulating. All Greek ECV4 isolates were closely related to the Spanish ECV4 strain. Genetic analysis of the VP1 gene can significantly contribute to the revelation of the endemic EV strains circulation pattern and their phylogenetic relationship with enteroviruses involved in epidemics of distant geographical areas at different time periods.</description><identifier>ISSN: 1386-6532</identifier><identifier>EISSN: 1873-5967</identifier><identifier>DOI: 10.1016/j.jcv.2009.07.019</identifier><identifier>PMID: 19699142</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Allergy and Immunology ; Aseptic meningitis ; Biological and medical sciences ; Child ; Child, Preschool ; Disease Outbreaks ; Echovirus ; Echovirus 4 ; Enterovirus ; Enterovirus - classification ; Enterovirus - genetics ; Enterovirus Infections ; Enterovirus Infections - cerebrospinal fluid ; Enterovirus Infections - epidemiology ; Enterovirus Infections - immunology ; Enterovirus Infections - virology ; Feces ; Feces - virology ; Female ; Fundamental and applied biological sciences. Psychology ; Greece ; Greece - epidemiology ; Human viral diseases ; Humans ; Infectious Disease ; Infectious diseases ; Life Sciences ; Male ; Medical sciences ; Meningitis, Aseptic ; Meningitis, Aseptic - cerebrospinal fluid ; Meningitis, Aseptic - epidemiology ; Meningitis, Aseptic - immunology ; Meningitis, Aseptic - virology ; Microbiology ; Microbiology and Parasitology ; Miscellaneous ; NASBA ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral ; RNA, Viral - analysis ; RNA, Viral - cerebrospinal fluid ; Self-Sustained Sequence Replication ; Self-Sustained Sequence Replication - methods ; Serotyping ; Viral diseases ; Virology ; VP1</subject><ispartof>J Clin Virol, 2009-11, Vol.46 (3), p.270-274</ispartof><rights>Elsevier B.V.</rights><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-a74e7362cd45bc19ed3e9d543a6f09b1a3674488ea23a5fba2ced9819df5ea1e3</citedby><cites>FETCH-LOGICAL-c506t-a74e7362cd45bc19ed3e9d543a6f09b1a3674488ea23a5fba2ced9819df5ea1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jcv.2009.07.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22102444$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19699142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://riip.hal.science/pasteur-00734685$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Logotheti, Maria</creatorcontrib><creatorcontrib>Pogka, Vasiliki</creatorcontrib><creatorcontrib>Horefti, Elina</creatorcontrib><creatorcontrib>Papadakos, Konstantinos</creatorcontrib><creatorcontrib>Giannaki, Maria</creatorcontrib><creatorcontrib>Pangalis, Anastasia</creatorcontrib><creatorcontrib>Sgouras, Dionyssios</creatorcontrib><creatorcontrib>Mentis, Andreas</creatorcontrib><title>Laboratory investigation and phylogenetic analysis of enteroviruses involved in an aseptic meningitis outbreak in Greece during the summer of 2007</title><title>J Clin Virol</title><addtitle>J Clin Virol</addtitle><description>Abstract Background Aseptic meningitis is the most commonly observed CNS infection and is mainly attributed to Non-Polio Enteroviruses (EV). Objective Identification and genetic analysis of the EV involved in the recent aseptic meningitis outbreak which occurred in Greece, during the summer of 2007. Study design In total, 213 CSF and faecal samples were examined for EV presence by culture, while enteroviral RNA detection was performed by nucleic acid sequence-based amplification assay (NASBA). EV strains were typed by seroneutralization, as well as nested RT-PCR followed by VP1-2A gene partial sequencing. Phylogenetic analysis was carried out for the identification of the genetic relatedness among the isolated EV strains. Results EV detection rate in CSF and faecal samples was 43.9% and 70.8%, respectively. EV serotyping and VP1 region analysis revealed the predominance of echovirus 4 (ECV4) serotype and the circulation of ECV6, 9, 14, 25, Coxsackie A6, A15, A24 and Coxsackie B1 serotypes. All ECV4 isolates presented a 98.7% similarity in nucleotide sequence, with a Spanish ECV4 strain, isolated during a meningitis outbreak in 2006. Conclusions It is the first time that ECV4 is associated with an aseptic meningitis outbreak in Greece, during which 9 different EV serotypes were co-circulating. All Greek ECV4 isolates were closely related to the Spanish ECV4 strain. Genetic analysis of the VP1 gene can significantly contribute to the revelation of the endemic EV strains circulation pattern and their phylogenetic relationship with enteroviruses involved in epidemics of distant geographical areas at different time periods.</description><subject>Allergy and Immunology</subject><subject>Aseptic meningitis</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Disease Outbreaks</subject><subject>Echovirus</subject><subject>Echovirus 4</subject><subject>Enterovirus</subject><subject>Enterovirus - classification</subject><subject>Enterovirus - genetics</subject><subject>Enterovirus Infections</subject><subject>Enterovirus Infections - cerebrospinal fluid</subject><subject>Enterovirus Infections - epidemiology</subject><subject>Enterovirus Infections - immunology</subject><subject>Enterovirus Infections - virology</subject><subject>Feces</subject><subject>Feces - virology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Greece</subject><subject>Greece - epidemiology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Meningitis, Aseptic</subject><subject>Meningitis, Aseptic - cerebrospinal fluid</subject><subject>Meningitis, Aseptic - epidemiology</subject><subject>Meningitis, Aseptic - immunology</subject><subject>Meningitis, Aseptic - virology</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Miscellaneous</subject><subject>NASBA</subject><subject>Phylogeny</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - cerebrospinal fluid</subject><subject>Self-Sustained Sequence Replication</subject><subject>Self-Sustained Sequence Replication - methods</subject><subject>Serotyping</subject><subject>Viral diseases</subject><subject>Virology</subject><subject>VP1</subject><issn>1386-6532</issn><issn>1873-5967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ks1u1DAUhSMEoqXwAGxQNsAqg_8Sx0KqVFXQIo3EAlhbN87NjKdJPNhOpLwGT4yjGRWJRVe27O9cH99zs-wtJRtKaPXpsDmYecMIURsiN4SqZ9klrSUvSlXJ52nP66qoSs4uslchHAihJRfyZXZBVaUUFewy-7OFxnmIzi-5HWcM0e4gWjfmMLb5cb_0bocjRmvSAfRLsCF3XY5jRO9m66eAYRW6fsY2bRKVQ8DjKhhwtOPOxlUyxcYjPKzEnUc0mLeTT7d53GMepmFAv9ZNf5Gvsxcd9AHfnNer7NfXLz9v74vt97tvtzfbwpSkigVIgZJXzLSibAxV2HJUbSk4VB1RDQVeSSHqGoFxKLsGmMFW1VS1XYlAkV9lxanuHnp99HYAv2gHVt_fbPURQsTJ6-SHi6ouZ5r4jyf-6N3vKXVKDzYY7HsY0U1BJ5BIJWqeyA9PkoyoarWWQHoCjXcheOwefVCi14j1QaeI9RqxJlKniJPm3bn41AzY_lOcM03A-zMAwUDfeRiNDY8cY5QwIUTiPp84TE2eLXodjMUxdcl6NFG3zj5p4_o_tentaNODD7hgOLjJp3kJmurANNE_1llcR5EoQrikhP8F1sbbcA</recordid><startdate>20091101</startdate><enddate>20091101</enddate><creator>Logotheti, Maria</creator><creator>Pogka, Vasiliki</creator><creator>Horefti, Elina</creator><creator>Papadakos, Konstantinos</creator><creator>Giannaki, Maria</creator><creator>Pangalis, Anastasia</creator><creator>Sgouras, Dionyssios</creator><creator>Mentis, Andreas</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20091101</creationdate><title>Laboratory investigation and phylogenetic analysis of enteroviruses involved in an aseptic meningitis outbreak in Greece during the summer of 2007</title><author>Logotheti, Maria ; Pogka, Vasiliki ; Horefti, Elina ; Papadakos, Konstantinos ; Giannaki, Maria ; Pangalis, Anastasia ; Sgouras, Dionyssios ; Mentis, Andreas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-a74e7362cd45bc19ed3e9d543a6f09b1a3674488ea23a5fba2ced9819df5ea1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Allergy and Immunology</topic><topic>Aseptic meningitis</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Disease Outbreaks</topic><topic>Echovirus</topic><topic>Echovirus 4</topic><topic>Enterovirus</topic><topic>Enterovirus - classification</topic><topic>Enterovirus - genetics</topic><topic>Enterovirus Infections</topic><topic>Enterovirus Infections - cerebrospinal fluid</topic><topic>Enterovirus Infections - epidemiology</topic><topic>Enterovirus Infections - immunology</topic><topic>Enterovirus Infections - virology</topic><topic>Feces</topic><topic>Feces - virology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Greece</topic><topic>Greece - epidemiology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Meningitis, Aseptic</topic><topic>Meningitis, Aseptic - cerebrospinal fluid</topic><topic>Meningitis, Aseptic - epidemiology</topic><topic>Meningitis, Aseptic - immunology</topic><topic>Meningitis, Aseptic - virology</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Miscellaneous</topic><topic>NASBA</topic><topic>Phylogeny</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral</topic><topic>RNA, Viral - analysis</topic><topic>RNA, Viral - cerebrospinal fluid</topic><topic>Self-Sustained Sequence Replication</topic><topic>Self-Sustained Sequence Replication - methods</topic><topic>Serotyping</topic><topic>Viral diseases</topic><topic>Virology</topic><topic>VP1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Logotheti, Maria</creatorcontrib><creatorcontrib>Pogka, Vasiliki</creatorcontrib><creatorcontrib>Horefti, Elina</creatorcontrib><creatorcontrib>Papadakos, Konstantinos</creatorcontrib><creatorcontrib>Giannaki, Maria</creatorcontrib><creatorcontrib>Pangalis, Anastasia</creatorcontrib><creatorcontrib>Sgouras, Dionyssios</creatorcontrib><creatorcontrib>Mentis, Andreas</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>J Clin Virol</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Logotheti, Maria</au><au>Pogka, Vasiliki</au><au>Horefti, Elina</au><au>Papadakos, Konstantinos</au><au>Giannaki, Maria</au><au>Pangalis, Anastasia</au><au>Sgouras, Dionyssios</au><au>Mentis, Andreas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laboratory investigation and phylogenetic analysis of enteroviruses involved in an aseptic meningitis outbreak in Greece during the summer of 2007</atitle><jtitle>J Clin Virol</jtitle><addtitle>J Clin Virol</addtitle><date>2009-11-01</date><risdate>2009</risdate><volume>46</volume><issue>3</issue><spage>270</spage><epage>274</epage><pages>270-274</pages><issn>1386-6532</issn><eissn>1873-5967</eissn><abstract>Abstract Background Aseptic meningitis is the most commonly observed CNS infection and is mainly attributed to Non-Polio Enteroviruses (EV). Objective Identification and genetic analysis of the EV involved in the recent aseptic meningitis outbreak which occurred in Greece, during the summer of 2007. Study design In total, 213 CSF and faecal samples were examined for EV presence by culture, while enteroviral RNA detection was performed by nucleic acid sequence-based amplification assay (NASBA). EV strains were typed by seroneutralization, as well as nested RT-PCR followed by VP1-2A gene partial sequencing. Phylogenetic analysis was carried out for the identification of the genetic relatedness among the isolated EV strains. Results EV detection rate in CSF and faecal samples was 43.9% and 70.8%, respectively. EV serotyping and VP1 region analysis revealed the predominance of echovirus 4 (ECV4) serotype and the circulation of ECV6, 9, 14, 25, Coxsackie A6, A15, A24 and Coxsackie B1 serotypes. All ECV4 isolates presented a 98.7% similarity in nucleotide sequence, with a Spanish ECV4 strain, isolated during a meningitis outbreak in 2006. Conclusions It is the first time that ECV4 is associated with an aseptic meningitis outbreak in Greece, during which 9 different EV serotypes were co-circulating. All Greek ECV4 isolates were closely related to the Spanish ECV4 strain. Genetic analysis of the VP1 gene can significantly contribute to the revelation of the endemic EV strains circulation pattern and their phylogenetic relationship with enteroviruses involved in epidemics of distant geographical areas at different time periods.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19699142</pmid><doi>10.1016/j.jcv.2009.07.019</doi><tpages>5</tpages></addata></record> |
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| ispartof | J Clin Virol, 2009-11, Vol.46 (3), p.270-274 |
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| subjects | Allergy and Immunology Aseptic meningitis Biological and medical sciences Child Child, Preschool Disease Outbreaks Echovirus Echovirus 4 Enterovirus Enterovirus - classification Enterovirus - genetics Enterovirus Infections Enterovirus Infections - cerebrospinal fluid Enterovirus Infections - epidemiology Enterovirus Infections - immunology Enterovirus Infections - virology Feces Feces - virology Female Fundamental and applied biological sciences. Psychology Greece Greece - epidemiology Human viral diseases Humans Infectious Disease Infectious diseases Life Sciences Male Medical sciences Meningitis, Aseptic Meningitis, Aseptic - cerebrospinal fluid Meningitis, Aseptic - epidemiology Meningitis, Aseptic - immunology Meningitis, Aseptic - virology Microbiology Microbiology and Parasitology Miscellaneous NASBA Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Viral RNA, Viral - analysis RNA, Viral - cerebrospinal fluid Self-Sustained Sequence Replication Self-Sustained Sequence Replication - methods Serotyping Viral diseases Virology VP1 |
| title | Laboratory investigation and phylogenetic analysis of enteroviruses involved in an aseptic meningitis outbreak in Greece during the summer of 2007 |
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