Isozyme-specific modes of activation of CTP: Phosphorylcholine cytidylyltransferase in Arabidopsis thaliana at low temperature
Arabidopsis thaliana increases cellular phosphatidylcholine (PC) content during cold acclimation by up-regulating PC biosynthesis. The A. thaliana genes CCT1 and CCT2 encode CTP:phosphorylcholine cytidylyltransferases (CCTs; EC 2.7.7.15), which regulate PC biosynthesis via the CDP-choline pathway. W...
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| creator | Inatsugi, R.(Tokyo Univ. (Japan)) Kawai, H Yamaoka, Y Yu, Y Sekiguchi, A Nakamura, M Nishida, I |
| description | Arabidopsis thaliana increases cellular phosphatidylcholine (PC) content during cold acclimation by up-regulating PC biosynthesis. The A. thaliana genes CCT1 and CCT2 encode CTP:phosphorylcholine cytidylyltransferases (CCTs; EC 2.7.7.15), which regulate PC biosynthesis via the CDP-choline pathway. We isolated the T-DNA-tagged knockout mutants cct1 and cct2 of A. thaliana (Wassilevskaja; WS). CCT activity in cct1 and cct2 plants accounted for 29 and 79% to the cellular CCT activity of WS plants, respectively. When plants were exposed to 2degC for 7 d, CCT activity increased in both cct1 and cct2 plants, and immunoblot analyses revealed that cct1 contained an increased level of CCT2 protein whereas cct2 exhibited little increase in CCT1 level. For each mutant grown at 23degC, CCT activity was mainly enriched in the particulate (15,000 x g pellet) and microsomal (150,000 x g pellet) fractions from rosette leaf homogenates. After exposure to cold, the particulate and microsomal fractions of cct1 plants had higher total CCT activity due to increased levels of CCT2; in contrast, the levels of CCT1 in cct2 plants remained unchanged in particulate and microsomal fractions despite a significant increase in the total CCT activity. We conclude that the CDP-choline pathway of A. thaliana is up-regulated at low temperature via isogene-specific modes: enhanced expression of CCT2 and post-translational activation/inactivation of CCT1 in membranes. PC levels were similarly maintained in both mutants and WS plants after 14d at 2degC, suggesting that either of the CCT genes is sufficient for PC biosynthesis at low temperature. |
| doi_str_mv | 10.1093/pcp/pcp115 |
| format | Article |
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(Japan)) ; Kawai, H ; Yamaoka, Y ; Yu, Y ; Sekiguchi, A ; Nakamura, M ; Nishida, I</creator><creatorcontrib>Inatsugi, R.(Tokyo Univ. (Japan)) ; Kawai, H ; Yamaoka, Y ; Yu, Y ; Sekiguchi, A ; Nakamura, M ; Nishida, I</creatorcontrib><description>Arabidopsis thaliana increases cellular phosphatidylcholine (PC) content during cold acclimation by up-regulating PC biosynthesis. The A. thaliana genes CCT1 and CCT2 encode CTP:phosphorylcholine cytidylyltransferases (CCTs; EC 2.7.7.15), which regulate PC biosynthesis via the CDP-choline pathway. We isolated the T-DNA-tagged knockout mutants cct1 and cct2 of A. thaliana (Wassilevskaja; WS). CCT activity in cct1 and cct2 plants accounted for 29 and 79% to the cellular CCT activity of WS plants, respectively. When plants were exposed to 2degC for 7 d, CCT activity increased in both cct1 and cct2 plants, and immunoblot analyses revealed that cct1 contained an increased level of CCT2 protein whereas cct2 exhibited little increase in CCT1 level. For each mutant grown at 23degC, CCT activity was mainly enriched in the particulate (15,000 x g pellet) and microsomal (150,000 x g pellet) fractions from rosette leaf homogenates. After exposure to cold, the particulate and microsomal fractions of cct1 plants had higher total CCT activity due to increased levels of CCT2; in contrast, the levels of CCT1 in cct2 plants remained unchanged in particulate and microsomal fractions despite a significant increase in the total CCT activity. We conclude that the CDP-choline pathway of A. thaliana is up-regulated at low temperature via isogene-specific modes: enhanced expression of CCT2 and post-translational activation/inactivation of CCT1 in membranes. PC levels were similarly maintained in both mutants and WS plants after 14d at 2degC, suggesting that either of the CCT genes is sufficient for PC biosynthesis at low temperature.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcp115</identifier><identifier>PMID: 19667100</identifier><language>eng</language><publisher>Japan: Oxford University Press</publisher><subject>ADN ; Amino Acid Sequence ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; ARABIDOPSIS THALIANA ; ARN ; CDP–choline pathway ; Cell Membrane - metabolism ; Choline-Phosphate Cytidylyltransferase - genetics ; Choline-Phosphate Cytidylyltransferase - metabolism ; Cold acclimation ; Cold Temperature ; DNA ; DNA, Bacterial - genetics ; DNA, Plant - genetics ; Gene Expression Regulation, Plant ; Gene Knockout Techniques ; ISOENZIMAS ; ISOENZYME ; ISOENZYMES ; Isoenzymes - genetics ; Isoenzymes - metabolism ; LIPIDE ; LIPIDOS ; LIPIDS ; Membrane expansion ; Molecular Sequence Data ; MUTANT ; MUTANTES ; MUTANTS ; Phosphati-dylcholine ; Post-translational regulation ; PROTEINAS ; PROTEINE ; PROTEINS ; RNA ; TEMPERATURA ; TEMPERATURE</subject><ispartof>Plant and cell physiology, 2009-10, Vol.50 (10), p.1727-1735</ispartof><rights>The Author 2009. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-a7f5853de62c8573b1aa10335800995bd16dd2ddb84c548b33e0583a02c0f2b63</citedby><cites>FETCH-LOGICAL-c504t-a7f5853de62c8573b1aa10335800995bd16dd2ddb84c548b33e0583a02c0f2b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19667100$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inatsugi, R.(Tokyo Univ. (Japan))</creatorcontrib><creatorcontrib>Kawai, H</creatorcontrib><creatorcontrib>Yamaoka, Y</creatorcontrib><creatorcontrib>Yu, Y</creatorcontrib><creatorcontrib>Sekiguchi, A</creatorcontrib><creatorcontrib>Nakamura, M</creatorcontrib><creatorcontrib>Nishida, I</creatorcontrib><title>Isozyme-specific modes of activation of CTP: Phosphorylcholine cytidylyltransferase in Arabidopsis thaliana at low temperature</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>Arabidopsis thaliana increases cellular phosphatidylcholine (PC) content during cold acclimation by up-regulating PC biosynthesis. The A. thaliana genes CCT1 and CCT2 encode CTP:phosphorylcholine cytidylyltransferases (CCTs; EC 2.7.7.15), which regulate PC biosynthesis via the CDP-choline pathway. We isolated the T-DNA-tagged knockout mutants cct1 and cct2 of A. thaliana (Wassilevskaja; WS). CCT activity in cct1 and cct2 plants accounted for 29 and 79% to the cellular CCT activity of WS plants, respectively. When plants were exposed to 2degC for 7 d, CCT activity increased in both cct1 and cct2 plants, and immunoblot analyses revealed that cct1 contained an increased level of CCT2 protein whereas cct2 exhibited little increase in CCT1 level. For each mutant grown at 23degC, CCT activity was mainly enriched in the particulate (15,000 x g pellet) and microsomal (150,000 x g pellet) fractions from rosette leaf homogenates. After exposure to cold, the particulate and microsomal fractions of cct1 plants had higher total CCT activity due to increased levels of CCT2; in contrast, the levels of CCT1 in cct2 plants remained unchanged in particulate and microsomal fractions despite a significant increase in the total CCT activity. We conclude that the CDP-choline pathway of A. thaliana is up-regulated at low temperature via isogene-specific modes: enhanced expression of CCT2 and post-translational activation/inactivation of CCT1 in membranes. PC levels were similarly maintained in both mutants and WS plants after 14d at 2degC, suggesting that either of the CCT genes is sufficient for PC biosynthesis at low temperature.</description><subject>ADN</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>ARABIDOPSIS THALIANA</subject><subject>ARN</subject><subject>CDP–choline pathway</subject><subject>Cell Membrane - metabolism</subject><subject>Choline-Phosphate Cytidylyltransferase - genetics</subject><subject>Choline-Phosphate Cytidylyltransferase - metabolism</subject><subject>Cold acclimation</subject><subject>Cold Temperature</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Plant - genetics</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Knockout Techniques</subject><subject>ISOENZIMAS</subject><subject>ISOENZYME</subject><subject>ISOENZYMES</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>LIPIDE</subject><subject>LIPIDOS</subject><subject>LIPIDS</subject><subject>Membrane expansion</subject><subject>Molecular Sequence Data</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>Phosphati-dylcholine</subject><subject>Post-translational regulation</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>RNA</subject><subject>TEMPERATURA</subject><subject>TEMPERATURE</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90c-L1DAUB_Agiju7evGu5CKCUH1pJk27t2Vwf7niCCuKl_CapE60bWqSqvXg326HDnrzEN578OF7-IaQRwxeMKj4y0EP-8eYuENWbC1ZVoHgd8kKgOcZyJIdkeMYvwDMO4f75IhVRSEZwIr8vor-19TZLA5Wu8Zp2nljI_UNRZ3cd0zO9_trc7s9pdudj8POh6nVO9-63lI9JWemdmpTwD42NmC01PX0LGDtjB-iizTtsHXYI8VEW_-DJtsNM0xjsA_IvQbbaB8e5gl5f_7qdnOZ3by9uNqc3WRawDplKBtRCm5sketSSF4zRAacixKgqkRtWGFMbkxdrrVYlzXnFkTJEXINTV4X_IQ8W3KH4L-NNibVuaht22Jv_RiV5GuQFRd7-XyROvgYg23UEFyHYVIM1L5uNVetlrpn_OQQO9adNf_ood8ZPF2AH4f_B2WLczHZn38lhq-qkFwKdfnxk3rz4WJTXr_O1bvZP158g17h5-Ciut7mcxnzF1dS8j_zQKMK</recordid><startdate>200910</startdate><enddate>200910</enddate><creator>Inatsugi, R.(Tokyo Univ. 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(Japan))</creatorcontrib><creatorcontrib>Kawai, H</creatorcontrib><creatorcontrib>Yamaoka, Y</creatorcontrib><creatorcontrib>Yu, Y</creatorcontrib><creatorcontrib>Sekiguchi, A</creatorcontrib><creatorcontrib>Nakamura, M</creatorcontrib><creatorcontrib>Nishida, I</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inatsugi, R.(Tokyo Univ. (Japan))</au><au>Kawai, H</au><au>Yamaoka, Y</au><au>Yu, Y</au><au>Sekiguchi, A</au><au>Nakamura, M</au><au>Nishida, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isozyme-specific modes of activation of CTP: Phosphorylcholine cytidylyltransferase in Arabidopsis thaliana at low temperature</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2009-10</date><risdate>2009</risdate><volume>50</volume><issue>10</issue><spage>1727</spage><epage>1735</epage><pages>1727-1735</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Arabidopsis thaliana increases cellular phosphatidylcholine (PC) content during cold acclimation by up-regulating PC biosynthesis. The A. thaliana genes CCT1 and CCT2 encode CTP:phosphorylcholine cytidylyltransferases (CCTs; EC 2.7.7.15), which regulate PC biosynthesis via the CDP-choline pathway. We isolated the T-DNA-tagged knockout mutants cct1 and cct2 of A. thaliana (Wassilevskaja; WS). CCT activity in cct1 and cct2 plants accounted for 29 and 79% to the cellular CCT activity of WS plants, respectively. When plants were exposed to 2degC for 7 d, CCT activity increased in both cct1 and cct2 plants, and immunoblot analyses revealed that cct1 contained an increased level of CCT2 protein whereas cct2 exhibited little increase in CCT1 level. For each mutant grown at 23degC, CCT activity was mainly enriched in the particulate (15,000 x g pellet) and microsomal (150,000 x g pellet) fractions from rosette leaf homogenates. After exposure to cold, the particulate and microsomal fractions of cct1 plants had higher total CCT activity due to increased levels of CCT2; in contrast, the levels of CCT1 in cct2 plants remained unchanged in particulate and microsomal fractions despite a significant increase in the total CCT activity. We conclude that the CDP-choline pathway of A. thaliana is up-regulated at low temperature via isogene-specific modes: enhanced expression of CCT2 and post-translational activation/inactivation of CCT1 in membranes. PC levels were similarly maintained in both mutants and WS plants after 14d at 2degC, suggesting that either of the CCT genes is sufficient for PC biosynthesis at low temperature.</abstract><cop>Japan</cop><pub>Oxford University Press</pub><pmid>19667100</pmid><doi>10.1093/pcp/pcp115</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | ADN Amino Acid Sequence Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism ARABIDOPSIS THALIANA ARN CDP–choline pathway Cell Membrane - metabolism Choline-Phosphate Cytidylyltransferase - genetics Choline-Phosphate Cytidylyltransferase - metabolism Cold acclimation Cold Temperature DNA DNA, Bacterial - genetics DNA, Plant - genetics Gene Expression Regulation, Plant Gene Knockout Techniques ISOENZIMAS ISOENZYME ISOENZYMES Isoenzymes - genetics Isoenzymes - metabolism LIPIDE LIPIDOS LIPIDS Membrane expansion Molecular Sequence Data MUTANT MUTANTES MUTANTS Phosphati-dylcholine Post-translational regulation PROTEINAS PROTEINE PROTEINS RNA TEMPERATURA TEMPERATURE |
| title | Isozyme-specific modes of activation of CTP: Phosphorylcholine cytidylyltransferase in Arabidopsis thaliana at low temperature |
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