Effects of Reconstituted Collagen Matrix on Fates of Mouse Embryonic Stem Cells Before and After Induction for Chondrogenic Differentiation
Embryonic stem (ES) cells are pluripotent cells with great potential in regenerative medicine. However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) ce...
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| Veröffentlicht in: | Tissue engineering. Part A 2009-10, Vol.15 (10), p.371-3085 |
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| creator | Yeung, Chiu W. Cheah, Kathryn Chan, Danny Chan, Barbara P. |
| description | Embryonic stem (ES) cells are pluripotent cells with great potential in regenerative medicine. However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) cells before and after induction for chondrogenic differentiation. Specifically, mES cells were encapsulated and cultured in 3D collagen microspheres and exposed to induction signals at different time points. Growth characteristics and differentiation status of mES cells were then evaluated. Collagen microspheres provided a suitable microenvironment supporting mES cell growth and maintaining their undifferentiated status for certain period of time. At later time points, the proportion of undifferentiated mES cells gradually decreased, accompanied by increasing proportions of mesenchymal progenitor cells. This suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Moreover, a lower initial collagen monomer concentration facilitated the differentiation of mES cells into chondrogenic lineages, while induction at a later time point associated with a more advanced stage of chondrogenic differentiation. This indicates that both the initial collagen concentration and the time to induce differentiation significantly affected the fates of mES cells. This study contributes to future development of ES cell–based therapies. |
| doi_str_mv | 10.1089/ten.tea.2008.0661 |
| format | Article |
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However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) cells before and after induction for chondrogenic differentiation. Specifically, mES cells were encapsulated and cultured in 3D collagen microspheres and exposed to induction signals at different time points. Growth characteristics and differentiation status of mES cells were then evaluated. Collagen microspheres provided a suitable microenvironment supporting mES cell growth and maintaining their undifferentiated status for certain period of time. At later time points, the proportion of undifferentiated mES cells gradually decreased, accompanied by increasing proportions of mesenchymal progenitor cells. This suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Moreover, a lower initial collagen monomer concentration facilitated the differentiation of mES cells into chondrogenic lineages, while induction at a later time point associated with a more advanced stage of chondrogenic differentiation. This indicates that both the initial collagen concentration and the time to induce differentiation significantly affected the fates of mES cells. This study contributes to future development of ES cell–based therapies.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2008.0661</identifier><identifier>PMID: 19331579</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Cartilage cells ; Cell differentiation ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Cellular biology ; Chondrogenesis ; Collagen ; Collagen - metabolism ; Collagen - pharmacology ; Embryonic stem cells ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - drug effects ; Extracellular Matrix - metabolism ; Flow Cytometry ; Immunohistochemistry ; Mice ; Microspheres ; Original Articles ; Physiological aspects ; Rodents ; Signal transduction ; SOX9 Transcription Factor - metabolism ; Stem cells ; Tissue engineering ; Tissue Engineering - methods</subject><ispartof>Tissue engineering. 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Part A</title><addtitle>Tissue Eng Part A</addtitle><description>Embryonic stem (ES) cells are pluripotent cells with great potential in regenerative medicine. However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) cells before and after induction for chondrogenic differentiation. Specifically, mES cells were encapsulated and cultured in 3D collagen microspheres and exposed to induction signals at different time points. Growth characteristics and differentiation status of mES cells were then evaluated. Collagen microspheres provided a suitable microenvironment supporting mES cell growth and maintaining their undifferentiated status for certain period of time. At later time points, the proportion of undifferentiated mES cells gradually decreased, accompanied by increasing proportions of mesenchymal progenitor cells. This suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Moreover, a lower initial collagen monomer concentration facilitated the differentiation of mES cells into chondrogenic lineages, while induction at a later time point associated with a more advanced stage of chondrogenic differentiation. This indicates that both the initial collagen concentration and the time to induce differentiation significantly affected the fates of mES cells. This study contributes to future development of ES cell–based therapies.</description><subject>Animals</subject><subject>Cartilage cells</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Chondrogenesis</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Collagen - pharmacology</subject><subject>Embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - drug effects</subject><subject>Extracellular Matrix - metabolism</subject><subject>Flow Cytometry</subject><subject>Immunohistochemistry</subject><subject>Mice</subject><subject>Microspheres</subject><subject>Original Articles</subject><subject>Physiological aspects</subject><subject>Rodents</subject><subject>Signal transduction</subject><subject>SOX9 Transcription Factor - metabolism</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkc9u1DAQxiMEomXhAbggc4HTBv9JHOe4hG2p1AoJeuAWOfG4uErsYjsSfQZemkl3BRIHhC3L1szvG3v8FcVLRktGVfsugy8z6JJTqkoqJXtUnLJWNFsh6q-Pf58rdlI8S-mWUkll0zwtTjAhWN20p8XPvbUw5kSCJZ9hDD5ll5cMhnRhmvQNeHKlc3Q_SPDkTGd4IK_CkoDs5yHeB-9G8iXDTDqYpkTegw0RiPaG7GyGSC68WcbsUI4J0n0L3sSAdVH2weHlEXx2egWeF0-snhK8OO6b4vpsf9193F5-Or_odpfbsWY0b9nQSjW0vNVtY6hgzADXIIANI7dqVEJRbisrpBmklkNlOIaV0rZqB6hBbIq3h7J3MXxfIOV-dmnEx2sP2FffiIo2quYcyTf_JDljnDLJEHz9F3gbluixiV61glcCx6YoD8yNnqB33oYc9YjTwOzw48E6jO94pWhbSUlRwA6CMYaUItj-LrpZx_ue0X71v0f_cel-9b9f_UfNq-NDlmEG80dxNByB5gCsYe395GCAmP-j9C-xwsEP</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Yeung, Chiu W.</creator><creator>Cheah, Kathryn</creator><creator>Chan, Danny</creator><creator>Chan, Barbara P.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Effects of Reconstituted Collagen Matrix on Fates of Mouse Embryonic Stem Cells Before and After Induction for Chondrogenic Differentiation</title><author>Yeung, Chiu W. ; Cheah, Kathryn ; Chan, Danny ; Chan, Barbara P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-1b968b929a97d0311de2ae3e1bc2f8c83802f4f36db6a6b4d22f888af49be5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Cartilage cells</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Cellular biology</topic><topic>Chondrogenesis</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Collagen - pharmacology</topic><topic>Embryonic stem cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - drug effects</topic><topic>Extracellular Matrix - metabolism</topic><topic>Flow Cytometry</topic><topic>Immunohistochemistry</topic><topic>Mice</topic><topic>Microspheres</topic><topic>Original Articles</topic><topic>Physiological aspects</topic><topic>Rodents</topic><topic>Signal transduction</topic><topic>SOX9 Transcription Factor - metabolism</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yeung, Chiu W.</creatorcontrib><creatorcontrib>Cheah, Kathryn</creatorcontrib><creatorcontrib>Chan, Danny</creatorcontrib><creatorcontrib>Chan, Barbara P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Tissue engineering. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yeung, Chiu W.</au><au>Cheah, Kathryn</au><au>Chan, Danny</au><au>Chan, Barbara P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Reconstituted Collagen Matrix on Fates of Mouse Embryonic Stem Cells Before and After Induction for Chondrogenic Differentiation</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>15</volume><issue>10</issue><spage>371</spage><epage>3085</epage><pages>371-3085</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>Embryonic stem (ES) cells are pluripotent cells with great potential in regenerative medicine. However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) cells before and after induction for chondrogenic differentiation. Specifically, mES cells were encapsulated and cultured in 3D collagen microspheres and exposed to induction signals at different time points. Growth characteristics and differentiation status of mES cells were then evaluated. Collagen microspheres provided a suitable microenvironment supporting mES cell growth and maintaining their undifferentiated status for certain period of time. At later time points, the proportion of undifferentiated mES cells gradually decreased, accompanied by increasing proportions of mesenchymal progenitor cells. This suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Moreover, a lower initial collagen monomer concentration facilitated the differentiation of mES cells into chondrogenic lineages, while induction at a later time point associated with a more advanced stage of chondrogenic differentiation. This indicates that both the initial collagen concentration and the time to induce differentiation significantly affected the fates of mES cells. This study contributes to future development of ES cell–based therapies.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>19331579</pmid><doi>10.1089/ten.tea.2008.0661</doi><tpages>2715</tpages></addata></record> |
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| subjects | Animals Cartilage cells Cell differentiation Cell Differentiation - drug effects Cell Differentiation - physiology Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Cellular biology Chondrogenesis Collagen Collagen - metabolism Collagen - pharmacology Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - drug effects Extracellular Matrix - metabolism Flow Cytometry Immunohistochemistry Mice Microspheres Original Articles Physiological aspects Rodents Signal transduction SOX9 Transcription Factor - metabolism Stem cells Tissue engineering Tissue Engineering - methods |
| title | Effects of Reconstituted Collagen Matrix on Fates of Mouse Embryonic Stem Cells Before and After Induction for Chondrogenic Differentiation |
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