Enhancement of Embryonic Stem Cell Differentiation Promoted by Avian Chorioallantoic Membranes
Avian chorioallantoic membrane (CAM) has been used as a model to explore angiogenesis and to study the microvasculature of transplanted tissues. Because CAM provides a vascular bed, cells can be implanted, and their development can be monitored and modified. We used the CAM model to study the differ...
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| Veröffentlicht in: | Tissue engineering. Part A 2009-10, Vol.15 (10), p.3193-3200 |
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| creator | Talavera-Adame, Dodanim Dafoe, Donald C. Ng, Tina T. Wachsmann-Hogiu, Sebastian Castillo-Henkel, Carlos Farkas, Daniel L. |
| description | Avian chorioallantoic membrane (CAM) has been used as a model to explore angiogenesis and to study the microvasculature of transplanted tissues. Because CAM provides a vascular bed, cells can be implanted, and their development can be monitored and modified. We used the CAM model to study the differentiation process of embryoid bodies (EBs) derived from mouse embryonic stem cells (ESCs) influenced by the CAM vascular bed. After EBs were incubated in CAM for 5 days, they underwent further differentiation and became tissue masses (TMs) of different morphologies from those that grew outside CAM. Immunohistochemical analysis of TMs demonstrated tissue-specific markers such as neurofilament light, CD34, collagen IV, cardiac myosin heavy chain (MHC), and cardiotin. Differentiated mouse blood vessels stained with anti-CD31 were found within the TMs, as well as blood vessels stained positive for QH1 and QCPN, markers for quail endothelial cells and perinuclear quail antigen, respectively. Quail erythrocytes inside mouse blood vessels suggested a connection between existing quail vessels and blood vessels growing inside the TMs as a result of EB differentiation. Therefore, CAM could be a suitable model to trigger and study the differentiation of EBs in close interaction with surrogate quail blood vessels. |
| doi_str_mv | 10.1089/ten.tea.2009.0024 |
| format | Article |
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Because CAM provides a vascular bed, cells can be implanted, and their development can be monitored and modified. We used the CAM model to study the differentiation process of embryoid bodies (EBs) derived from mouse embryonic stem cells (ESCs) influenced by the CAM vascular bed. After EBs were incubated in CAM for 5 days, they underwent further differentiation and became tissue masses (TMs) of different morphologies from those that grew outside CAM. Immunohistochemical analysis of TMs demonstrated tissue-specific markers such as neurofilament light, CD34, collagen IV, cardiac myosin heavy chain (MHC), and cardiotin. Differentiated mouse blood vessels stained with anti-CD31 were found within the TMs, as well as blood vessels stained positive for QH1 and QCPN, markers for quail endothelial cells and perinuclear quail antigen, respectively. Quail erythrocytes inside mouse blood vessels suggested a connection between existing quail vessels and blood vessels growing inside the TMs as a result of EB differentiation. Therefore, CAM could be a suitable model to trigger and study the differentiation of EBs in close interaction with surrogate quail blood vessels.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2009.0024</identifier><identifier>PMID: 19364272</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Actinin - metabolism ; Animals ; Antigens, CD34 - metabolism ; Birds ; Cardiac Myosins - metabolism ; Cell differentiation ; Cell Differentiation - physiology ; Cell Line ; Cell membranes ; Cellular biology ; Chorioallantoic Membrane - metabolism ; Collagen Type IV - metabolism ; Coturnix ; Embryo, Mammalian - cytology ; Embryo, Mammalian - metabolism ; Embryonic stem cells ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Immunohistochemistry ; Membranes ; Mice ; Original Articles ; Physiological aspects ; Stem cells ; Tissue engineering ; Tissue Engineering - methods</subject><ispartof>Tissue engineering. 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Part A</title><addtitle>Tissue Eng Part A</addtitle><description>Avian chorioallantoic membrane (CAM) has been used as a model to explore angiogenesis and to study the microvasculature of transplanted tissues. Because CAM provides a vascular bed, cells can be implanted, and their development can be monitored and modified. We used the CAM model to study the differentiation process of embryoid bodies (EBs) derived from mouse embryonic stem cells (ESCs) influenced by the CAM vascular bed. After EBs were incubated in CAM for 5 days, they underwent further differentiation and became tissue masses (TMs) of different morphologies from those that grew outside CAM. Immunohistochemical analysis of TMs demonstrated tissue-specific markers such as neurofilament light, CD34, collagen IV, cardiac myosin heavy chain (MHC), and cardiotin. Differentiated mouse blood vessels stained with anti-CD31 were found within the TMs, as well as blood vessels stained positive for QH1 and QCPN, markers for quail endothelial cells and perinuclear quail antigen, respectively. Quail erythrocytes inside mouse blood vessels suggested a connection between existing quail vessels and blood vessels growing inside the TMs as a result of EB differentiation. Therefore, CAM could be a suitable model to trigger and study the differentiation of EBs in close interaction with surrogate quail blood vessels.</description><subject>Actinin - metabolism</subject><subject>Animals</subject><subject>Antigens, CD34 - metabolism</subject><subject>Birds</subject><subject>Cardiac Myosins - metabolism</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Cell membranes</subject><subject>Cellular biology</subject><subject>Chorioallantoic Membrane - metabolism</subject><subject>Collagen Type IV - metabolism</subject><subject>Coturnix</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Immunohistochemistry</subject><subject>Membranes</subject><subject>Mice</subject><subject>Original Articles</subject><subject>Physiological aspects</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkUuLFDEUhQtxcB76A9xI3DirLvOqpLJs2h4VRhRUcGVIpW6cDFXJTJIe6H9vim4UXAwSLgn3fudww2malwS3BPfqbYHQFjAtxVi1GFP-pDkjiskVY92Pp3_enJw25znfYiywkPJZc1oHglNJz5qf23BjgoUZQkHRoe08pH0M3qKvBWa0gWlC77xzkCrgTfExoC8pzrHAiIY9Wj94E9DmJiYfzTSZUGLVfoJqYwLk582JM1OGF8f7ovl-tf22-bC6_vz-42Z9vbKc87IaFFGKOi65UGx0rB8cMQwT4dzABio61hPWjXY0tBOyY7yTjIjRWDv2uGOEXTSXB9-7FO93kIuefbawLARxl7VkHMu-w7KSbx4lKSGMc4or-Pof8DbuUqi_0L1iRHElKtMemF9mAu2DiyUZW88Is7cxgPO1v6a8x4oLSauAHAQ2xZwTOH2X_GzSXhOsl0x1zbSW0Uumesm0al4dF9kNM4x_FccQKyAPwNI2IUweBkjlP6x_A31Gr_Y</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Talavera-Adame, Dodanim</creator><creator>Dafoe, Donald C.</creator><creator>Ng, Tina T.</creator><creator>Wachsmann-Hogiu, Sebastian</creator><creator>Castillo-Henkel, Carlos</creator><creator>Farkas, Daniel L.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Enhancement of Embryonic Stem Cell Differentiation Promoted by Avian Chorioallantoic Membranes</title><author>Talavera-Adame, Dodanim ; Dafoe, Donald C. ; Ng, Tina T. ; Wachsmann-Hogiu, Sebastian ; Castillo-Henkel, Carlos ; Farkas, Daniel L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-b91992f474693df38bf1a3016ffb3b26538135dcda256753457316daccd805313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Actinin - metabolism</topic><topic>Animals</topic><topic>Antigens, CD34 - metabolism</topic><topic>Birds</topic><topic>Cardiac Myosins - metabolism</topic><topic>Cell differentiation</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Cell membranes</topic><topic>Cellular biology</topic><topic>Chorioallantoic Membrane - metabolism</topic><topic>Collagen Type IV - metabolism</topic><topic>Coturnix</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Embryonic stem cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - metabolism</topic><topic>Immunohistochemistry</topic><topic>Membranes</topic><topic>Mice</topic><topic>Original Articles</topic><topic>Physiological aspects</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Talavera-Adame, Dodanim</creatorcontrib><creatorcontrib>Dafoe, Donald C.</creatorcontrib><creatorcontrib>Ng, Tina T.</creatorcontrib><creatorcontrib>Wachsmann-Hogiu, Sebastian</creatorcontrib><creatorcontrib>Castillo-Henkel, Carlos</creatorcontrib><creatorcontrib>Farkas, Daniel L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Tissue engineering. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Talavera-Adame, Dodanim</au><au>Dafoe, Donald C.</au><au>Ng, Tina T.</au><au>Wachsmann-Hogiu, Sebastian</au><au>Castillo-Henkel, Carlos</au><au>Farkas, Daniel L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of Embryonic Stem Cell Differentiation Promoted by Avian Chorioallantoic Membranes</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>15</volume><issue>10</issue><spage>3193</spage><epage>3200</epage><pages>3193-3200</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>Avian chorioallantoic membrane (CAM) has been used as a model to explore angiogenesis and to study the microvasculature of transplanted tissues. Because CAM provides a vascular bed, cells can be implanted, and their development can be monitored and modified. We used the CAM model to study the differentiation process of embryoid bodies (EBs) derived from mouse embryonic stem cells (ESCs) influenced by the CAM vascular bed. After EBs were incubated in CAM for 5 days, they underwent further differentiation and became tissue masses (TMs) of different morphologies from those that grew outside CAM. Immunohistochemical analysis of TMs demonstrated tissue-specific markers such as neurofilament light, CD34, collagen IV, cardiac myosin heavy chain (MHC), and cardiotin. Differentiated mouse blood vessels stained with anti-CD31 were found within the TMs, as well as blood vessels stained positive for QH1 and QCPN, markers for quail endothelial cells and perinuclear quail antigen, respectively. Quail erythrocytes inside mouse blood vessels suggested a connection between existing quail vessels and blood vessels growing inside the TMs as a result of EB differentiation. Therefore, CAM could be a suitable model to trigger and study the differentiation of EBs in close interaction with surrogate quail blood vessels.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>19364272</pmid><doi>10.1089/ten.tea.2009.0024</doi><tpages>8</tpages></addata></record> |
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| subjects | Actinin - metabolism Animals Antigens, CD34 - metabolism Birds Cardiac Myosins - metabolism Cell differentiation Cell Differentiation - physiology Cell Line Cell membranes Cellular biology Chorioallantoic Membrane - metabolism Collagen Type IV - metabolism Coturnix Embryo, Mammalian - cytology Embryo, Mammalian - metabolism Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Immunohistochemistry Membranes Mice Original Articles Physiological aspects Stem cells Tissue engineering Tissue Engineering - methods |
| title | Enhancement of Embryonic Stem Cell Differentiation Promoted by Avian Chorioallantoic Membranes |
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