NCAM expression induces neurogenesis in vivo
Neural cell adhesion molecule (NCAM) plays an important role during neural development and in the adult brain, whereby most functions of NCAM have been ascribed to its unique polysialic acid (PSA) modification. Recently we presented evidence suggesting that expression of NCAM in vivo interferes with...
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| Veröffentlicht in: | The European journal of neuroscience 2009-10, Vol.30 (7), p.1209-1218 |
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| creator | Boutin, Camille Schmitz, Brigitte Cremer, Harold Diestel, Simone |
| description | Neural cell adhesion molecule (NCAM) plays an important role during neural development and in the adult brain, whereby most functions of NCAM have been ascribed to its unique polysialic acid (PSA) modification. Recently we presented evidence suggesting that expression of NCAM in vivo interferes with the maintenance of forebrain neuronal stem cells. We here aimed at investigating the fate of cells generated from NCAM‐overexpressing stem cells in postnatal mouse brain and at elucidating the functional domains of NCAM mediating this effect. We show that ectopic expression of the NCAM140 isoform in radial glia and type C cells induces an increase in cell proliferation and consequently the presence of additional neuronal type A cells in the rostral migratory stream. A mutant NCAM protein comprising only fibronectin type III repeats and immunoglobulin‐like domain 5 was sufficient to induce this effect. Furthermore, we show that the neurogenic effect is independent of PSA, as transgenic NCAM is not polysialylated in radial glia and type C cells. These results suggest that heterophilic interactions of NCAM with other components of the cell membrane must be involved. |
| doi_str_mv | 10.1111/j.1460-9568.2009.06928.x |
| format | Article |
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Recently we presented evidence suggesting that expression of NCAM in vivo interferes with the maintenance of forebrain neuronal stem cells. We here aimed at investigating the fate of cells generated from NCAM‐overexpressing stem cells in postnatal mouse brain and at elucidating the functional domains of NCAM mediating this effect. We show that ectopic expression of the NCAM140 isoform in radial glia and type C cells induces an increase in cell proliferation and consequently the presence of additional neuronal type A cells in the rostral migratory stream. A mutant NCAM protein comprising only fibronectin type III repeats and immunoglobulin‐like domain 5 was sufficient to induce this effect. Furthermore, we show that the neurogenic effect is independent of PSA, as transgenic NCAM is not polysialylated in radial glia and type C cells. 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Recently we presented evidence suggesting that expression of NCAM in vivo interferes with the maintenance of forebrain neuronal stem cells. We here aimed at investigating the fate of cells generated from NCAM‐overexpressing stem cells in postnatal mouse brain and at elucidating the functional domains of NCAM mediating this effect. We show that ectopic expression of the NCAM140 isoform in radial glia and type C cells induces an increase in cell proliferation and consequently the presence of additional neuronal type A cells in the rostral migratory stream. A mutant NCAM protein comprising only fibronectin type III repeats and immunoglobulin‐like domain 5 was sufficient to induce this effect. Furthermore, we show that the neurogenic effect is independent of PSA, as transgenic NCAM is not polysialylated in radial glia and type C cells. These results suggest that heterophilic interactions of NCAM with other components of the cell membrane must be involved.</description><subject>Animals</subject><subject>Brain - physiology</subject><subject>Cell Adhesion Molecules, Neuronal - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>heterophilic NCAM interactions</subject><subject>Humans</subject><subject>in vivo electroporation of postnatal mice</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Mice, Knockout</subject><subject>Mice, Transgenic</subject><subject>Mutation</subject><subject>Neural Cell Adhesion Molecules - chemistry</subject><subject>Neural Cell Adhesion Molecules - genetics</subject><subject>Neural Cell Adhesion Molecules - metabolism</subject><subject>Neurogenesis - physiology</subject><subject>Neuroglia - physiology</subject><subject>Neurons - physiology</subject><subject>olfactory neurogenesis</subject><subject>Protein Isoforms - metabolism</subject><subject>PSA-NCAM</subject><subject>Rats</subject><subject>Stem Cell Niche - physiology</subject><subject>Stem Cells - physiology</subject><issn>0953-816X</issn><issn>1460-9568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1PwkAQhjdGI4j-BdOTXmzdj3Y_Dh4IQdAAJkYj8bIp26kpQotdiuXfuxWiN-NcdrLzvDPJg5BHcEBcXc8DEnLsq4jLgGKsAswVlUF9gNo_g0PUxipiviR82kIn1s4xxpKH0TFqESWkjARuo6tJrzv2oF6VYG1W5F6WJ5UB6-VQlcUb5GAz6z69TbYpTtFRGi8snO3fDnq-7T_1hv7oYXDX6458E0ohfaqMUdQkIQkxF5ImMpYJ5wozkzIgEkhqODM0lTSNcWJmlCTYyJiBm0TxjHXQ5W7vqiw-KrBrvcysgcUizqGorBYsxEJQwh158SdJCYkcShwod6ApC2tLSPWqzJZxudUE68apnutGnW7U6cap_naqaxc939-oZktIfoN7iQ642QGf2QK2_16s-_eTpnN5f5fP7Brqn3xcvmsumIj0y2Sg1TQcv_LHoR6yL--Bk68</recordid><startdate>200910</startdate><enddate>200910</enddate><creator>Boutin, Camille</creator><creator>Schmitz, Brigitte</creator><creator>Cremer, Harold</creator><creator>Diestel, Simone</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200910</creationdate><title>NCAM expression induces neurogenesis in vivo</title><author>Boutin, Camille ; Schmitz, Brigitte ; Cremer, Harold ; Diestel, Simone</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4878-29cc92cd41406782d8a8d66903cf3e18e1fc63c2f82fa0dcb21d0c8a3ee1f5ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Brain - physiology</topic><topic>Cell Adhesion Molecules, Neuronal - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>heterophilic NCAM interactions</topic><topic>Humans</topic><topic>in vivo electroporation of postnatal mice</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Mice, Knockout</topic><topic>Mice, Transgenic</topic><topic>Mutation</topic><topic>Neural Cell Adhesion Molecules - chemistry</topic><topic>Neural Cell Adhesion Molecules - genetics</topic><topic>Neural Cell Adhesion Molecules - metabolism</topic><topic>Neurogenesis - physiology</topic><topic>Neuroglia - physiology</topic><topic>Neurons - physiology</topic><topic>olfactory neurogenesis</topic><topic>Protein Isoforms - metabolism</topic><topic>PSA-NCAM</topic><topic>Rats</topic><topic>Stem Cell Niche - physiology</topic><topic>Stem Cells - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boutin, Camille</creatorcontrib><creatorcontrib>Schmitz, Brigitte</creatorcontrib><creatorcontrib>Cremer, Harold</creatorcontrib><creatorcontrib>Diestel, Simone</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The European journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boutin, Camille</au><au>Schmitz, Brigitte</au><au>Cremer, Harold</au><au>Diestel, Simone</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NCAM expression induces neurogenesis in vivo</atitle><jtitle>The European journal of neuroscience</jtitle><addtitle>Eur J Neurosci</addtitle><date>2009-10</date><risdate>2009</risdate><volume>30</volume><issue>7</issue><spage>1209</spage><epage>1218</epage><pages>1209-1218</pages><issn>0953-816X</issn><eissn>1460-9568</eissn><abstract>Neural cell adhesion molecule (NCAM) plays an important role during neural development and in the adult brain, whereby most functions of NCAM have been ascribed to its unique polysialic acid (PSA) modification. Recently we presented evidence suggesting that expression of NCAM in vivo interferes with the maintenance of forebrain neuronal stem cells. We here aimed at investigating the fate of cells generated from NCAM‐overexpressing stem cells in postnatal mouse brain and at elucidating the functional domains of NCAM mediating this effect. We show that ectopic expression of the NCAM140 isoform in radial glia and type C cells induces an increase in cell proliferation and consequently the presence of additional neuronal type A cells in the rostral migratory stream. A mutant NCAM protein comprising only fibronectin type III repeats and immunoglobulin‐like domain 5 was sufficient to induce this effect. Furthermore, we show that the neurogenic effect is independent of PSA, as transgenic NCAM is not polysialylated in radial glia and type C cells. 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| subjects | Animals Brain - physiology Cell Adhesion Molecules, Neuronal - metabolism Cell Line, Tumor Cell Proliferation heterophilic NCAM interactions Humans in vivo electroporation of postnatal mice Mice Mice, Inbred Strains Mice, Knockout Mice, Transgenic Mutation Neural Cell Adhesion Molecules - chemistry Neural Cell Adhesion Molecules - genetics Neural Cell Adhesion Molecules - metabolism Neurogenesis - physiology Neuroglia - physiology Neurons - physiology olfactory neurogenesis Protein Isoforms - metabolism PSA-NCAM Rats Stem Cell Niche - physiology Stem Cells - physiology |
| title | NCAM expression induces neurogenesis in vivo |
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