Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter
As the main structural protein of oil body,OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulati...
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| Veröffentlicht in: | Science China. Life sciences 2009-09, Vol.52 (9), p.854-863 |
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| creator | Che, NanYing Yang, Yang Li, YanDong Wang, LiLi Huang, Ping Gao, Yin An, ChengCai |
| description | As the main structural protein of oil body,OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here,we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN,responsible for seed specificity and LEC2 activation,was determined by 5’-deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays,mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity,while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore,we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity. |
| doi_str_mv | 10.1007/s11427-009-0119-z |
| format | Article |
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OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here,we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN,responsible for seed specificity and LEC2 activation,was determined by 5’-deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays,mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity,while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore,we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity.</description><identifier>ISSN: 1674-7305</identifier><identifier>ISSN: 1006-9305</identifier><identifier>EISSN: 1869-1889</identifier><identifier>EISSN: 1862-2798</identifier><identifier>DOI: 10.1007/s11427-009-0119-z</identifier><identifier>PMID: 19802745</identifier><language>eng</language><publisher>Beijing: Science China Press</publisher><subject>Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Base Sequence ; Binding Sites - genetics ; Biomedical and Life Sciences ; DNA Primers - genetics ; DNA, Plant - genetics ; DNA, Plant - metabolism ; domain ; element ; expression ; factor ; gene ; Gene Expression Regulation, Plant ; Genes, Plant ; LEC2 ; Life Sciences ; Molecular Sequence Data ; OLEOSIN ; Plants, Genetically Modified ; Promoter Regions, Genetic ; Protein Binding ; seed-specific ; Sequence Deletion ; Sequence Homology, Nucleic Acid ; transcription ; Transcription Factors - metabolism</subject><ispartof>Science China. Life sciences, 2009-09, Vol.52 (9), p.854-863</ispartof><rights>Science in China Press and Springer Berlin Heidelberg 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-7f7d8e4c91f0bf76ecc42ad5469c69574f6801672c6d6150809f15adb0c9e3053</citedby><cites>FETCH-LOGICAL-c398t-7f7d8e4c91f0bf76ecc42ad5469c69574f6801672c6d6150809f15adb0c9e3053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/60112X/60112X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19802745$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Che, NanYing</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Li, YanDong</creatorcontrib><creatorcontrib>Wang, LiLi</creatorcontrib><creatorcontrib>Huang, Ping</creatorcontrib><creatorcontrib>Gao, Yin</creatorcontrib><creatorcontrib>An, ChengCai</creatorcontrib><title>Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter</title><title>Science China. Life sciences</title><addtitle>SCI CHINA SER C</addtitle><addtitle>Science China: Life Science</addtitle><description>As the main structural protein of oil body,OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here,we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN,responsible for seed specificity and LEC2 activation,was determined by 5’-deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays,mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity,while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore,we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>DNA Primers - genetics</subject><subject>DNA, Plant - genetics</subject><subject>DNA, Plant - metabolism</subject><subject>domain</subject><subject>element</subject><subject>expression</subject><subject>factor</subject><subject>gene</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant</subject><subject>LEC2</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>OLEOSIN</subject><subject>Plants, Genetically Modified</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>seed-specific</subject><subject>Sequence Deletion</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>transcription</subject><subject>Transcription Factors - metabolism</subject><issn>1674-7305</issn><issn>1006-9305</issn><issn>1869-1889</issn><issn>1862-2798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kctOGzEUhi3UqiDgAbpBVrvoasDXsb1EUQpIUSMVuujKmniOg1FmnNgzvfD0eJRIVCzqzbGt7_zn8iP0kZJLSoi6ypQKpipCTEUoNdXzETqhujYV1dq8K_daiUpxIo_Rec5PpBzOCVPqAzqmRpebkCeonXsfXIB-wIv5jOHGDeFXM4TY4-jxcjFf3t99w_BnmyDn6TfBbgzlgYffEfcQ1o-rmEK_xt9_YthAV5QyLlwoYZtiFwdIZ-i9bzYZzg_xFP34On-Y3VaL5c3d7HpROW70UCmvWg3CGerJyqsanBOsaaWojauNVMLXmpSxmKvbmkqiifFUNu2KOANlUH6Kvux1S-HdCHmwXcgONpumhzhmq7ggSio6kZ_ekE9xTH1pzjImpNKCiQLRPeRSzDmBt9sUuib9tZTYyQO798AWD-zkgX0uORcH4XHVQfuacdh4AdgeyNtpbZBeK_9P9fOhk8fYr3cl759WCBdacyX5C5u7nJg</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Che, NanYing</creator><creator>Yang, Yang</creator><creator>Li, YanDong</creator><creator>Wang, LiLi</creator><creator>Huang, Ping</creator><creator>Gao, Yin</creator><creator>An, ChengCai</creator><general>Science China Press</general><general>Springer Nature B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20090901</creationdate><title>Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter</title><author>Che, NanYing ; Yang, Yang ; Li, YanDong ; Wang, LiLi ; Huang, Ping ; Gao, Yin ; An, ChengCai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-7f7d8e4c91f0bf76ecc42ad5469c69574f6801672c6d6150809f15adb0c9e3053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Binding Sites - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>DNA Primers - genetics</topic><topic>DNA, Plant - genetics</topic><topic>DNA, Plant - metabolism</topic><topic>domain</topic><topic>element</topic><topic>expression</topic><topic>factor</topic><topic>gene</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes, Plant</topic><topic>LEC2</topic><topic>Life Sciences</topic><topic>Molecular Sequence Data</topic><topic>OLEOSIN</topic><topic>Plants, Genetically Modified</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>seed-specific</topic><topic>Sequence Deletion</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>transcription</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Che, NanYing</creatorcontrib><creatorcontrib>Yang, Yang</creatorcontrib><creatorcontrib>Li, YanDong</creatorcontrib><creatorcontrib>Wang, LiLi</creatorcontrib><creatorcontrib>Huang, Ping</creatorcontrib><creatorcontrib>Gao, Yin</creatorcontrib><creatorcontrib>An, ChengCai</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Science China. Life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Che, NanYing</au><au>Yang, Yang</au><au>Li, YanDong</au><au>Wang, LiLi</au><au>Huang, Ping</au><au>Gao, Yin</au><au>An, ChengCai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter</atitle><jtitle>Science China. Life sciences</jtitle><stitle>SCI CHINA SER C</stitle><addtitle>Science China: Life Science</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>52</volume><issue>9</issue><spage>854</spage><epage>863</epage><pages>854-863</pages><issn>1674-7305</issn><issn>1006-9305</issn><eissn>1869-1889</eissn><eissn>1862-2798</eissn><abstract>As the main structural protein of oil body,OLEOSIN is highly expressed only during seed development. OLEOSIN promoter is a very useful tool for seed-specific gene engineering and seed bioreactor designing. The B3 domain transcription factor leafy cotyledon2 (LEC2) plays an important role in regulating seed development and seed-specific gene expression. Here,we first report how seed-specific B3 domain transcription factor leafy cotyledon2 (LEC2) efficiently activates OLEOSIN expression. The central promoter region of OLEOSIN,responsible for seed specificity and LEC2 activation,was determined by 5’-deletion analysis. Binding experiments in yeast cells and electrophoretic mobility shift assays showed that LEC2 specifically bound to two conserved RY elements in this region. In transient expression assays,mutation in either RY element dramatically reduced LEC2 activation of OLEOSIN promoter activity,while double mutation abolished it. Analysis of the distribution of RY elements in seed-specific genes activated by LEC2 also supported the idea that genes containing neighboring RY elements responded strongly to LEC2 activation. Therefore,we conclude that two neighboring RY elements are essential for efficient LEC2 activation of OLEOSIN expression. These findings will help us better utilize seed-specific promoter activity.</abstract><cop>Beijing</cop><pub>Science China Press</pub><pmid>19802745</pmid><doi>10.1007/s11427-009-0119-z</doi><tpages>10</tpages></addata></record> |
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| subjects | Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Base Sequence Binding Sites - genetics Biomedical and Life Sciences DNA Primers - genetics DNA, Plant - genetics DNA, Plant - metabolism domain element expression factor gene Gene Expression Regulation, Plant Genes, Plant LEC2 Life Sciences Molecular Sequence Data OLEOSIN Plants, Genetically Modified Promoter Regions, Genetic Protein Binding seed-specific Sequence Deletion Sequence Homology, Nucleic Acid transcription Transcription Factors - metabolism |
| title | Efficient LEC2 activation of OLEOSIN expression requires two neighboring RY elements on its promoter |
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