Universal Primer-Multiplex PCR Approach for Simultaneous Detection of Escherichia coli, Listeria monocytogenes, and Salmonella spp. in Food Samples

Escherichia coli, Listeria monocytogenes, and Salmonella spp. are 3 kinds of the most important food-borne human pathogens. Traditional microbiological analysis is labor-intensive, time-consuming, and easily contaminated, thus producing false positive signals; it also involves much subjectivity judg...

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Veröffentlicht in:	Journal of food science 2009-10, Vol.74 (8), p.M446-M452
Hauptverfasser:	Yuan, Yanfang, Xu, Wentao, Zhai, Zhifang, Shi, Hui, Luo, Yunbo, Chen, Zhuojun, Huang, Kunlun
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals bacterial contamination Biological and medical sciences Chemical reactions Dairy Products - microbiology DNA Primers E coli Eggs - microbiology Escherichia coli Escherichia coli - isolation & purification food analysis food contamination Food contamination & poisoning Food industries Food Microbiology food pathogens Food safety Food science Foodborne Diseases - prevention & control Fundamental and applied biological sciences. Psychology General aspects Limit of Detection Listeria monocytogenes Listeria monocytogenes - isolation & purification Meat - microbiology Methods of analysis, processing and quality control, regulation, standards microbial detection Microbiology Pathogens polymerase chain reaction Polymerase Chain Reaction - methods rapid methods Salmonella Salmonella - isolation & purification Salmonella spp simultaneous detection universal primer-multiplex PCR universal primer-multiplex polymerase chain reaction
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