Quantification of the Bla(CMY-2) in Feces from Beef Feedlot Cattle Administered Three Different Doses of Ceftiofur in a Longitudinal Controlled Field Trial

The objective of this longitudinal controlled trial was to quantitatively compare carriage of a gene encoding for ceftiofur-resistance (bla(CMY-2)), standardized to a reference gene (16SrRNA), among total community DNA extracted from fecal samples collected from cattle treated with three different d...

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Veröffentlicht in:	Foodborne pathogens and disease 2009-10, Vol.6 (8), p.917-924
Hauptverfasser:	Alali, W.Q, Scott, H.M, Norby, B, Gebreyes, W, Loneragan, G.H
Format:	Artikel
Sprache:	eng
Schlagworte:	animal breeding Animal Husbandry - methods Animals Anti-Bacterial Agents - administration & dosage Anti-Bacterial Agents - pharmacology antibiotic resistance antibiotics Bacteria - genetics Bacteria - growth & development beef cattle beta-Lactamases - analysis beta-Lactamases - genetics Carrier State - diagnosis Carrier State - drug therapy Carrier State - veterinary Cattle - microbiology cattle breeds cattle housing ceftiofur Cephalosporin Resistance - genetics Cephalosporins - administration & dosage Cephalosporins - pharmacology community DNA DNA DNA, Bacterial - genetics DNA, Bacterial - isolation & purification dosage dose response feces Feces - microbiology feedlots field experimentation gene banks gene copy genetic resistance Longitudinal Studies Male molecular sequence data pens Polymerase Chain Reaction quantitative analysis rearing Rectum - microbiology reference genes resistance mechanisms RNA, Ribosomal, 16S - analysis RNA, Ribosomal, 16S - genetics selection index selection pressure Selection, Genetic - drug effects steers Time Factors
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