Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues
Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly i...
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| Veröffentlicht in: | Cell biochemistry and function 2009-10, Vol.27 (7), p.440-447 |
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| creator | Toyoda, Mito Matsubara, Yoshinori Lin, Konghua Sugimachi, Keizou Furue, Masutaka |
| description | Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue‐derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31−CD34+CD45−CD90‐CD105−CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such population was observed in OM adipose tissue. On the other hand, CD31−CD34+CD45−CD90−CD105−CD146− population, which is an undefined cell population, were found solely in OM adipose tissue. Overall, the SC adipose tissue contained more ATDCs than OM adipose tissue, while OM adipose tissue contained more blood‐derived cells. Regarding to the inherent properties of fat cell progenitors from the two depots, adipose‐derived stem cells (ADSCs) from SC had higher capacity to differentiate into both adipogenic and osteogenic lineages than those from OM, regardless of that the proliferation rates of ADSCs from both depots were similar. The higher differentiation capacity of ADSCs from SC adipose tissue suggests that SC tissue is more suitable cell source for regenerative medicine than OM adipose tissue. Copyright © 2009 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/cbf.1591 |
| format | Article |
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These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue‐derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31−CD34+CD45−CD90‐CD105−CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such population was observed in OM adipose tissue. On the other hand, CD31−CD34+CD45−CD90−CD105−CD146− population, which is an undefined cell population, were found solely in OM adipose tissue. Overall, the SC adipose tissue contained more ATDCs than OM adipose tissue, while OM adipose tissue contained more blood‐derived cells. Regarding to the inherent properties of fat cell progenitors from the two depots, adipose‐derived stem cells (ADSCs) from SC had higher capacity to differentiate into both adipogenic and osteogenic lineages than those from OM, regardless of that the proliferation rates of ADSCs from both depots were similar. The higher differentiation capacity of ADSCs from SC adipose tissue suggests that SC tissue is more suitable cell source for regenerative medicine than OM adipose tissue. Copyright © 2009 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0263-6484</identifier><identifier>EISSN: 1099-0844</identifier><identifier>DOI: 10.1002/cbf.1591</identifier><identifier>PMID: 19691084</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>adipose derived stem cells ; adipose tissue-derived cells ; Aged ; Cell Differentiation ; Cell Proliferation ; Cell Survival ; Cells, Cultured ; Colony-Forming Units Assay ; Female ; Flow Cytometry ; Humans ; Kinetics ; Male ; metabolic function ; Middle Aged ; omental adipose tissue ; Omentum - cytology ; Osteogenesis ; subcutaneous adipose tissue ; Subcutaneous Fat - cytology</subject><ispartof>Cell biochemistry and function, 2009-10, Vol.27 (7), p.440-447</ispartof><rights>Copyright © 2009 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4241-d1053e558efc7ce68efba49867da298a6dd0e43801542d13bb6c995fd8fe93e73</citedby><cites>FETCH-LOGICAL-c4241-d1053e558efc7ce68efba49867da298a6dd0e43801542d13bb6c995fd8fe93e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbf.1591$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbf.1591$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19691084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toyoda, Mito</creatorcontrib><creatorcontrib>Matsubara, Yoshinori</creatorcontrib><creatorcontrib>Lin, Konghua</creatorcontrib><creatorcontrib>Sugimachi, Keizou</creatorcontrib><creatorcontrib>Furue, Masutaka</creatorcontrib><title>Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues</title><title>Cell biochemistry and function</title><addtitle>Cell Biochem. Funct</addtitle><description>Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue‐derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31−CD34+CD45−CD90‐CD105−CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such population was observed in OM adipose tissue. On the other hand, CD31−CD34+CD45−CD90−CD105−CD146− population, which is an undefined cell population, were found solely in OM adipose tissue. Overall, the SC adipose tissue contained more ATDCs than OM adipose tissue, while OM adipose tissue contained more blood‐derived cells. Regarding to the inherent properties of fat cell progenitors from the two depots, adipose‐derived stem cells (ADSCs) from SC had higher capacity to differentiate into both adipogenic and osteogenic lineages than those from OM, regardless of that the proliferation rates of ADSCs from both depots were similar. The higher differentiation capacity of ADSCs from SC adipose tissue suggests that SC tissue is more suitable cell source for regenerative medicine than OM adipose tissue. Copyright © 2009 John Wiley & Sons, Ltd.</description><subject>adipose derived stem cells</subject><subject>adipose tissue-derived cells</subject><subject>Aged</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Colony-Forming Units Assay</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Male</subject><subject>metabolic function</subject><subject>Middle Aged</subject><subject>omental adipose tissue</subject><subject>Omentum - cytology</subject><subject>Osteogenesis</subject><subject>subcutaneous adipose tissue</subject><subject>Subcutaneous Fat - cytology</subject><issn>0263-6484</issn><issn>1099-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10EtL5TAYBuAgI3q8gL9Auhs31aRJ02Y5U7xy0I0XcBPS5CtG2-ZM0nr79eZ4igOCq4_Aw5uXF6E9gg8JxtmRrptDkguyhmYEC5HikrFfaIYzTlPOSraJtkJ4xBgLTvEG2iSCCxLRDIXqQXmlB_D2XQ3W9YnqTaJdt1Dehvh0TaKMXbgAyWBDGCE10T5DRNC2IWm865KHsVN9EsZaj4PqwY3hM8Z10A-q_RYQdtB6o9oAu9PdRjcnx9fVWTq_Oj2v_sxTzTJGUkNwTiHPS2h0oYHHWysmSl4YlYlScWMwMFpikrPMEFrXXAuRN6ZsQFAo6Db6vcpdePcv_jvIzoZl61VFWVCGORd8KQ9WUnsXgodGLrztlH-TBMvlwjIuLJcLR7o_hY51B-Y_nCaNIF2BF9vC249Bsvp7MgVO3oYBXr-88k8yNityeXd5Ku8vb-_ovLqQ9_QDWEKWuQ</recordid><startdate>200910</startdate><enddate>200910</enddate><creator>Toyoda, Mito</creator><creator>Matsubara, Yoshinori</creator><creator>Lin, Konghua</creator><creator>Sugimachi, Keizou</creator><creator>Furue, Masutaka</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200910</creationdate><title>Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues</title><author>Toyoda, Mito ; Matsubara, Yoshinori ; Lin, Konghua ; Sugimachi, Keizou ; Furue, Masutaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4241-d1053e558efc7ce68efba49867da298a6dd0e43801542d13bb6c995fd8fe93e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>adipose derived stem cells</topic><topic>adipose tissue-derived cells</topic><topic>Aged</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Colony-Forming Units Assay</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Male</topic><topic>metabolic function</topic><topic>Middle Aged</topic><topic>omental adipose tissue</topic><topic>Omentum - cytology</topic><topic>Osteogenesis</topic><topic>subcutaneous adipose tissue</topic><topic>Subcutaneous Fat - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Toyoda, Mito</creatorcontrib><creatorcontrib>Matsubara, Yoshinori</creatorcontrib><creatorcontrib>Lin, Konghua</creatorcontrib><creatorcontrib>Sugimachi, Keizou</creatorcontrib><creatorcontrib>Furue, Masutaka</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biochemistry and function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Toyoda, Mito</au><au>Matsubara, Yoshinori</au><au>Lin, Konghua</au><au>Sugimachi, Keizou</au><au>Furue, Masutaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues</atitle><jtitle>Cell biochemistry and function</jtitle><addtitle>Cell Biochem. Funct</addtitle><date>2009-10</date><risdate>2009</risdate><volume>27</volume><issue>7</issue><spage>440</spage><epage>447</epage><pages>440-447</pages><issn>0263-6484</issn><eissn>1099-0844</eissn><abstract>Different fat depots contribute differently to disease and function. These differences may be due to the regional variation in cell types and inherent properties of fat cell progenitors. To address the differences of cell types in the adipose tissue from different depots, the phenotypes of freshly isolated adipose tissue‐derived cells (ATDCs) from subcutaneous (SC) and omental (OM) adipose tissues were compared using flow cytometry. Our results showed that CD31−CD34+CD45−CD90‐CD105−CD146+ population, containing vascular smooth muscle cells and pericytes, was specifically defined in the SC adipose tissue while no such population was observed in OM adipose tissue. On the other hand, CD31−CD34+CD45−CD90−CD105−CD146− population, which is an undefined cell population, were found solely in OM adipose tissue. Overall, the SC adipose tissue contained more ATDCs than OM adipose tissue, while OM adipose tissue contained more blood‐derived cells. Regarding to the inherent properties of fat cell progenitors from the two depots, adipose‐derived stem cells (ADSCs) from SC had higher capacity to differentiate into both adipogenic and osteogenic lineages than those from OM, regardless of that the proliferation rates of ADSCs from both depots were similar. The higher differentiation capacity of ADSCs from SC adipose tissue suggests that SC tissue is more suitable cell source for regenerative medicine than OM adipose tissue. Copyright © 2009 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>19691084</pmid><doi>10.1002/cbf.1591</doi><tpages>8</tpages></addata></record> |
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| subjects | adipose derived stem cells adipose tissue-derived cells Aged Cell Differentiation Cell Proliferation Cell Survival Cells, Cultured Colony-Forming Units Assay Female Flow Cytometry Humans Kinetics Male metabolic function Middle Aged omental adipose tissue Omentum - cytology Osteogenesis subcutaneous adipose tissue Subcutaneous Fat - cytology |
| title | Characterization and comparison of adipose tissue-derived cells from human subcutaneous and omental adipose tissues |
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