Simultaneous determination of phytoestrogens and key metabolites in breast cancer patients’ urine by liquid chromatography–tandem mass spectrometry
A novel, selective and sensitive liquid chromatography–tandem mass spectrometry method has been developed and validated for the simultaneous determination of phytoestrogens and their key metabolites in human urine in this study. This method includes internal standard (IS) screening, analytical sampl...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2009-12, Vol.50 (5), p.939-946 |
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| container_issue | 5 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Yu, Jing Wu, Qian Qiao, Shanlei Yu, Zeping Jin, Nianzu Yu, Boyang |
| description | A novel, selective and sensitive liquid chromatography–tandem mass spectrometry method has been developed and validated for the simultaneous determination of phytoestrogens and their key metabolites in human urine in this study. This method includes internal standard (IS) screening, analytical sample preparation procedure establishment, and linear range investigation. The analytical sample was extracted by liquid–liquid extraction from urine sample. The phytoestrogens and related key metabolites were separated with Agilent Zorbax Eclipse XDB-C18 chromatographic column using methanol and water as mobile phase. The Quattro premier MICROMASS mass spectrometer in negative ion selected reaction monitoring (SRM) mode using electrospray ionization was applied to detect the phytoestrogens and key metabolites. To validate the developed liquid chromatography–tandem mass spectrometry method, the intra- and inter-day precisions, specificity, sensitivity, reproducibility, and sample detective concentration range were evaluated. This is the first reported phytoestrogens analysis and validation study that demonstrates the feasibility of using liquid chromatography–electrospray ionization mass spectrometry to simultaneously analyze ten analytes including both phytoestrogens and their key metabolites in urine samples collected for epidemiological studies in human. |
| doi_str_mv | 10.1016/j.jpba.2009.06.032 |
| format | Article |
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This method includes internal standard (IS) screening, analytical sample preparation procedure establishment, and linear range investigation. The analytical sample was extracted by liquid–liquid extraction from urine sample. The phytoestrogens and related key metabolites were separated with Agilent Zorbax Eclipse XDB-C18 chromatographic column using methanol and water as mobile phase. The Quattro premier MICROMASS mass spectrometer in negative ion selected reaction monitoring (SRM) mode using electrospray ionization was applied to detect the phytoestrogens and key metabolites. To validate the developed liquid chromatography–tandem mass spectrometry method, the intra- and inter-day precisions, specificity, sensitivity, reproducibility, and sample detective concentration range were evaluated. This is the first reported phytoestrogens analysis and validation study that demonstrates the feasibility of using liquid chromatography–electrospray ionization mass spectrometry to simultaneously analyze ten analytes including both phytoestrogens and their key metabolites in urine samples collected for epidemiological studies in human.</description><identifier>ISSN: 0731-7085</identifier><identifier>EISSN: 1873-264X</identifier><identifier>DOI: 10.1016/j.jpba.2009.06.032</identifier><identifier>PMID: 19595530</identifier><identifier>CODEN: JPBADA</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Breast cancer ; Breast Neoplasms - urine ; Calibration ; Case-Control Studies ; Chemistry, Pharmaceutical - methods ; Chromatography - methods ; Chromatography, Liquid - methods ; Female ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Humans ; Liquid chromatography–tandem mass spectrometry ; Medical sciences ; Methanol - chemistry ; Models, Chemical ; Pharmacology. Drug treatments ; Phytoestrogens ; Phytoestrogens - metabolism ; Phytoestrogens - urine ; Quality Control ; Reproducibility of Results ; Tandem Mass Spectrometry - methods ; Time Factors ; Urine ; Water - chemistry</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2009-12, Vol.50 (5), p.939-946</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-eeab9f49cb18c22c98dbb752e4702678228eed81b13828b8259c5c74661f12ff3</citedby><cites>FETCH-LOGICAL-c416t-eeab9f49cb18c22c98dbb752e4702678228eed81b13828b8259c5c74661f12ff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0731708509003872$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22013365$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19595530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Jing</creatorcontrib><creatorcontrib>Wu, Qian</creatorcontrib><creatorcontrib>Qiao, Shanlei</creatorcontrib><creatorcontrib>Yu, Zeping</creatorcontrib><creatorcontrib>Jin, Nianzu</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><title>Simultaneous determination of phytoestrogens and key metabolites in breast cancer patients’ urine by liquid chromatography–tandem mass spectrometry</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>A novel, selective and sensitive liquid chromatography–tandem mass spectrometry method has been developed and validated for the simultaneous determination of phytoestrogens and their key metabolites in human urine in this study. This method includes internal standard (IS) screening, analytical sample preparation procedure establishment, and linear range investigation. The analytical sample was extracted by liquid–liquid extraction from urine sample. The phytoestrogens and related key metabolites were separated with Agilent Zorbax Eclipse XDB-C18 chromatographic column using methanol and water as mobile phase. The Quattro premier MICROMASS mass spectrometer in negative ion selected reaction monitoring (SRM) mode using electrospray ionization was applied to detect the phytoestrogens and key metabolites. To validate the developed liquid chromatography–tandem mass spectrometry method, the intra- and inter-day precisions, specificity, sensitivity, reproducibility, and sample detective concentration range were evaluated. This is the first reported phytoestrogens analysis and validation study that demonstrates the feasibility of using liquid chromatography–electrospray ionization mass spectrometry to simultaneously analyze ten analytes including both phytoestrogens and their key metabolites in urine samples collected for epidemiological studies in human.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - urine</subject><subject>Calibration</subject><subject>Case-Control Studies</subject><subject>Chemistry, Pharmaceutical - methods</subject><subject>Chromatography - methods</subject><subject>Chromatography, Liquid - methods</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Liquid chromatography–tandem mass spectrometry</subject><subject>Medical sciences</subject><subject>Methanol - chemistry</subject><subject>Models, Chemical</subject><subject>Pharmacology. Drug treatments</subject><subject>Phytoestrogens</subject><subject>Phytoestrogens - metabolism</subject><subject>Phytoestrogens - urine</subject><subject>Quality Control</subject><subject>Reproducibility of Results</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Time Factors</subject><subject>Urine</subject><subject>Water - chemistry</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhi0EotPCC7BA3kBXGXyJHUdiU1XcpEosAImdZTsnrYckTm0HKbu-AwvE6_VJ8GhGsOvKm-_85_j_EHpByZYSKt_strvZmi0jpN0SuSWcPUIbqhpeMVl_f4w2pOG0aogSJ-g0pR0hRNC2fopOaCtaITjZoN9f_LgM2UwQloQ7yBBHP5nsw4RDj-ebNQdIOYZrmBI2U4d_wIpHyMaGwWdI2E_YRjApY2cmBxHPZRqmnO7v_uAl-gmwXfHgbxffYXcTw2hyuI6mRN_f_SqbOxjxaFLCaQZXNpXwuD5DT3ozJHh-fM_Qt_fvvl5-rK4-f_h0eXFVuZrKXAEY2_Z16yxVjjHXqs7aRjCoG8JkoxhTAJ2ilnLFlFVMtE64ppaS9pT1PT9D54fcOYbbpfxUjz45GIZDI7rhNRGqlayQrx8kGS1F85YUkB1AF0NKEXo9Rz-auGpK9F6c3um9OL0Xp4nURVwZenlMX-wI3f-Ro6kCvDoCJjkz9LGU7dM_jjFCOZeicG8PHJTWfnqIOrmiw0HnY6lXd8E_dMdf0bK9Ew</recordid><startdate>20091205</startdate><enddate>20091205</enddate><creator>Yu, Jing</creator><creator>Wu, Qian</creator><creator>Qiao, Shanlei</creator><creator>Yu, Zeping</creator><creator>Jin, Nianzu</creator><creator>Yu, Boyang</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20091205</creationdate><title>Simultaneous determination of phytoestrogens and key metabolites in breast cancer patients’ urine by liquid chromatography–tandem mass spectrometry</title><author>Yu, Jing ; Wu, Qian ; Qiao, Shanlei ; Yu, Zeping ; Jin, Nianzu ; Yu, Boyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-eeab9f49cb18c22c98dbb752e4702678228eed81b13828b8259c5c74661f12ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - urine</topic><topic>Calibration</topic><topic>Case-Control Studies</topic><topic>Chemistry, Pharmaceutical - methods</topic><topic>Chromatography - methods</topic><topic>Chromatography, Liquid - methods</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Liquid chromatography–tandem mass spectrometry</topic><topic>Medical sciences</topic><topic>Methanol - chemistry</topic><topic>Models, Chemical</topic><topic>Pharmacology. Drug treatments</topic><topic>Phytoestrogens</topic><topic>Phytoestrogens - metabolism</topic><topic>Phytoestrogens - urine</topic><topic>Quality Control</topic><topic>Reproducibility of Results</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Time Factors</topic><topic>Urine</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Jing</creatorcontrib><creatorcontrib>Wu, Qian</creatorcontrib><creatorcontrib>Qiao, Shanlei</creatorcontrib><creatorcontrib>Yu, Zeping</creatorcontrib><creatorcontrib>Jin, Nianzu</creatorcontrib><creatorcontrib>Yu, Boyang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Jing</au><au>Wu, Qian</au><au>Qiao, Shanlei</au><au>Yu, Zeping</au><au>Jin, Nianzu</au><au>Yu, Boyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of phytoestrogens and key metabolites in breast cancer patients’ urine by liquid chromatography–tandem mass spectrometry</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2009-12-05</date><risdate>2009</risdate><volume>50</volume><issue>5</issue><spage>939</spage><epage>946</epage><pages>939-946</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>A novel, selective and sensitive liquid chromatography–tandem mass spectrometry method has been developed and validated for the simultaneous determination of phytoestrogens and their key metabolites in human urine in this study. This method includes internal standard (IS) screening, analytical sample preparation procedure establishment, and linear range investigation. The analytical sample was extracted by liquid–liquid extraction from urine sample. The phytoestrogens and related key metabolites were separated with Agilent Zorbax Eclipse XDB-C18 chromatographic column using methanol and water as mobile phase. The Quattro premier MICROMASS mass spectrometer in negative ion selected reaction monitoring (SRM) mode using electrospray ionization was applied to detect the phytoestrogens and key metabolites. To validate the developed liquid chromatography–tandem mass spectrometry method, the intra- and inter-day precisions, specificity, sensitivity, reproducibility, and sample detective concentration range were evaluated. This is the first reported phytoestrogens analysis and validation study that demonstrates the feasibility of using liquid chromatography–electrospray ionization mass spectrometry to simultaneously analyze ten analytes including both phytoestrogens and their key metabolites in urine samples collected for epidemiological studies in human.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19595530</pmid><doi>10.1016/j.jpba.2009.06.032</doi><tpages>8</tpages></addata></record> |
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| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Breast cancer Breast Neoplasms - urine Calibration Case-Control Studies Chemistry, Pharmaceutical - methods Chromatography - methods Chromatography, Liquid - methods Female Fundamental and applied biological sciences. Psychology General pharmacology Humans Liquid chromatography–tandem mass spectrometry Medical sciences Methanol - chemistry Models, Chemical Pharmacology. Drug treatments Phytoestrogens Phytoestrogens - metabolism Phytoestrogens - urine Quality Control Reproducibility of Results Tandem Mass Spectrometry - methods Time Factors Urine Water - chemistry |
| title | Simultaneous determination of phytoestrogens and key metabolites in breast cancer patients’ urine by liquid chromatography–tandem mass spectrometry |
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