A high-throughput test to detect C.E.R.A. doping in blood
C.E.R.A., a continuous erythropoietin receptor activator, is a new third-generation erythropoiesis-stimulating agent (ESA) that has recently been linked with abuse in endurance sports. In order to combat this new form of doping, we examined an enzyme-linked immunosorbent assay (ELISA) designed to de...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2009-12, Vol.50 (5), p.954-958 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
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| creator | Lamon, Séverine Giraud, Sylvain Egli, Léonie Smolander, Jessica Jarsch, Michael Stubenrauch, Kay-Gunnar Hellwig, Alice Saugy, Martial Robinson, Neil |
| description | C.E.R.A., a continuous erythropoietin receptor activator, is a new third-generation erythropoiesis-stimulating agent (ESA) that has recently been linked with abuse in endurance sports. In order to combat this new form of doping, we examined an enzyme-linked immunosorbent assay (ELISA) designed to detect the presence of C.E.R.A. in serum samples. The performance of the assay was evaluated using a pilot excretion study that involved six subjects receiving C.E.R.A. Validation data demonstrated an excellent reproducibility and ensured the applicability of the assay for anti-doping purposes. To maximize the chances of detecting the drug in serum samples, we propose the use of this specific ELISA test as a high-throughput screening method, combined with a classic isoelectric focusing test as a confirmatory assay. This strategy should make C.E.R.A. abuse relatively easy to detect, thereby preventing the future use of this drug as a doping agent. |
| doi_str_mv | 10.1016/j.jpba.2009.06.038 |
| format | Article |
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In order to combat this new form of doping, we examined an enzyme-linked immunosorbent assay (ELISA) designed to detect the presence of C.E.R.A. in serum samples. The performance of the assay was evaluated using a pilot excretion study that involved six subjects receiving C.E.R.A. Validation data demonstrated an excellent reproducibility and ensured the applicability of the assay for anti-doping purposes. To maximize the chances of detecting the drug in serum samples, we propose the use of this specific ELISA test as a high-throughput screening method, combined with a classic isoelectric focusing test as a confirmatory assay. 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Drug treatments ; Polyethylene Glycols - analysis ; Recombinant Proteins - chemistry ; Reproducibility of Results ; ROC Curve ; Sensitivity and Specificity ; Substance Abuse Detection - methods</subject><ispartof>Journal of pharmaceutical and biomedical analysis, 2009-12, Vol.50 (5), p.954-958</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-cb25f86f70051b9085430986da37989fad66d77f1bcc1cce74221f181316277e3</citedby><cites>FETCH-LOGICAL-c385t-cb25f86f70051b9085430986da37989fad66d77f1bcc1cce74221f181316277e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jpba.2009.06.038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22013367$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19625154$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lamon, Séverine</creatorcontrib><creatorcontrib>Giraud, Sylvain</creatorcontrib><creatorcontrib>Egli, Léonie</creatorcontrib><creatorcontrib>Smolander, Jessica</creatorcontrib><creatorcontrib>Jarsch, Michael</creatorcontrib><creatorcontrib>Stubenrauch, Kay-Gunnar</creatorcontrib><creatorcontrib>Hellwig, Alice</creatorcontrib><creatorcontrib>Saugy, Martial</creatorcontrib><creatorcontrib>Robinson, Neil</creatorcontrib><title>A high-throughput test to detect C.E.R.A. doping in blood</title><title>Journal of pharmaceutical and biomedical analysis</title><addtitle>J Pharm Biomed Anal</addtitle><description>C.E.R.A., a continuous erythropoietin receptor activator, is a new third-generation erythropoiesis-stimulating agent (ESA) that has recently been linked with abuse in endurance sports. In order to combat this new form of doping, we examined an enzyme-linked immunosorbent assay (ELISA) designed to detect the presence of C.E.R.A. in serum samples. The performance of the assay was evaluated using a pilot excretion study that involved six subjects receiving C.E.R.A. Validation data demonstrated an excellent reproducibility and ensured the applicability of the assay for anti-doping purposes. To maximize the chances of detecting the drug in serum samples, we propose the use of this specific ELISA test as a high-throughput screening method, combined with a classic isoelectric focusing test as a confirmatory assay. This strategy should make C.E.R.A. abuse relatively easy to detect, thereby preventing the future use of this drug as a doping agent.</description><subject>Adult</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Blood doping</subject><subject>C.E.R.A</subject><subject>Doping in Sports</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Erythropoietin</subject><subject>Erythropoietin - analysis</subject><subject>Erythropoietin - blood</subject><subject>Erythropoietin - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Isoelectric Focusing - methods</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Polyethylene Glycols - analysis</subject><subject>Recombinant Proteins - chemistry</subject><subject>Reproducibility of Results</subject><subject>ROC Curve</subject><subject>Sensitivity and Specificity</subject><subject>Substance Abuse Detection - methods</subject><issn>0731-7085</issn><issn>1873-264X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEURYMoWqt_wIXMRlxNzMdMkgE3pdQPKAii4C5kkkybMp2MyYzgvzelRXdu3tuc-97lAHCFEcQIs7sN3PS1ggShCiIGERVHYIIFpzlhxccxmCBOcc6RKM_AeYwbhFCJq-IUnOGKkRKXxQRUs2ztVut8WAc_rtb9OGSDjWn4zNjB6iGbwwV8hTOYGd-7bpW5Lqtb780FOGlUG-3lYU_B-8Pibf6UL18en-ezZa6pKIdc16RsBGv47nldpTIFRZVgRlFeiapRhjHDeYNrrbHWlheE4AYLTDEjnFs6Bbf7u33wn2PqJrcuatu2qrN-jJLTApWCCZZIsid18DEG28g-uK0K3xIjuTMmN3JnTO6MScRkMpZC14fzY7215i9yUJSAmwOgolZtE1SnXfzlCEGYUsYTd7_nbJLx5WyQUTvbaWtcSB6l8e6_Hj_0NIZJ</recordid><startdate>20091205</startdate><enddate>20091205</enddate><creator>Lamon, Séverine</creator><creator>Giraud, Sylvain</creator><creator>Egli, Léonie</creator><creator>Smolander, Jessica</creator><creator>Jarsch, Michael</creator><creator>Stubenrauch, Kay-Gunnar</creator><creator>Hellwig, Alice</creator><creator>Saugy, Martial</creator><creator>Robinson, Neil</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091205</creationdate><title>A high-throughput test to detect C.E.R.A. doping in blood</title><author>Lamon, Séverine ; Giraud, Sylvain ; Egli, Léonie ; Smolander, Jessica ; Jarsch, Michael ; Stubenrauch, Kay-Gunnar ; Hellwig, Alice ; Saugy, Martial ; Robinson, Neil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-cb25f86f70051b9085430986da37989fad66d77f1bcc1cce74221f181316277e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Blood doping</topic><topic>C.E.R.A</topic><topic>Doping in Sports</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Erythropoietin</topic><topic>Erythropoietin - analysis</topic><topic>Erythropoietin - blood</topic><topic>Erythropoietin - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Isoelectric Focusing - methods</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Polyethylene Glycols - analysis</topic><topic>Recombinant Proteins - chemistry</topic><topic>Reproducibility of Results</topic><topic>ROC Curve</topic><topic>Sensitivity and Specificity</topic><topic>Substance Abuse Detection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lamon, Séverine</creatorcontrib><creatorcontrib>Giraud, Sylvain</creatorcontrib><creatorcontrib>Egli, Léonie</creatorcontrib><creatorcontrib>Smolander, Jessica</creatorcontrib><creatorcontrib>Jarsch, Michael</creatorcontrib><creatorcontrib>Stubenrauch, Kay-Gunnar</creatorcontrib><creatorcontrib>Hellwig, Alice</creatorcontrib><creatorcontrib>Saugy, Martial</creatorcontrib><creatorcontrib>Robinson, Neil</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lamon, Séverine</au><au>Giraud, Sylvain</au><au>Egli, Léonie</au><au>Smolander, Jessica</au><au>Jarsch, Michael</au><au>Stubenrauch, Kay-Gunnar</au><au>Hellwig, Alice</au><au>Saugy, Martial</au><au>Robinson, Neil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A high-throughput test to detect C.E.R.A. doping in blood</atitle><jtitle>Journal of pharmaceutical and biomedical analysis</jtitle><addtitle>J Pharm Biomed Anal</addtitle><date>2009-12-05</date><risdate>2009</risdate><volume>50</volume><issue>5</issue><spage>954</spage><epage>958</epage><pages>954-958</pages><issn>0731-7085</issn><eissn>1873-264X</eissn><coden>JPBADA</coden><abstract>C.E.R.A., a continuous erythropoietin receptor activator, is a new third-generation erythropoiesis-stimulating agent (ESA) that has recently been linked with abuse in endurance sports. In order to combat this new form of doping, we examined an enzyme-linked immunosorbent assay (ELISA) designed to detect the presence of C.E.R.A. in serum samples. The performance of the assay was evaluated using a pilot excretion study that involved six subjects receiving C.E.R.A. Validation data demonstrated an excellent reproducibility and ensured the applicability of the assay for anti-doping purposes. To maximize the chances of detecting the drug in serum samples, we propose the use of this specific ELISA test as a high-throughput screening method, combined with a classic isoelectric focusing test as a confirmatory assay. This strategy should make C.E.R.A. abuse relatively easy to detect, thereby preventing the future use of this drug as a doping agent.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19625154</pmid><doi>10.1016/j.jpba.2009.06.038</doi><tpages>5</tpages></addata></record> |
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| subjects | Adult Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Blood doping C.E.R.A Doping in Sports ELISA Enzyme-Linked Immunosorbent Assay - methods Erythropoietin Erythropoietin - analysis Erythropoietin - blood Erythropoietin - metabolism Fundamental and applied biological sciences. Psychology General pharmacology Humans Isoelectric Focusing - methods Male Medical sciences Pharmacology. Drug treatments Polyethylene Glycols - analysis Recombinant Proteins - chemistry Reproducibility of Results ROC Curve Sensitivity and Specificity Substance Abuse Detection - methods |
| title | A high-throughput test to detect C.E.R.A. doping in blood |
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