Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep ( Ovis aries)
A new species, Cryptosporidium xiaoi, is described from sheep. Oocysts of C. xiaoi, previously identified as the Cryptosporidium bovis-like genotype or as C. bovis from sheep in Spain, Tunisia, United Kingdom, and the United States are recorded as such in GenBank ( EU408314– EU408317, EU327318– EU32...
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| Veröffentlicht in: | Veterinary parasitology 2009-10, Vol.164 (2), p.192-200 |
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| creator | Fayer, Ronald Santín, Mónica |
| description | A new species,
Cryptosporidium xiaoi, is described from sheep. Oocysts of
C. xiaoi, previously identified as the
Cryptosporidium bovis-like genotype or as
C. bovis from sheep in Spain, Tunisia, United Kingdom, and the United States are recorded as such in GenBank (
EU408314–
EU408317,
EU327318–
EU327320,
EF362478,
EF514234,
DQ991389, and
EF158461). Oocysts obtained from naturally infected sheep were infectious for a lamb and oocysts from that lamb were infectious for three other lambs. The prepatent period for
C. xiaoi in these four
Cryptosporidium-naïve lambs was 7–8 days and the patent period was 13–15 days. Oocysts are similar to those of
C. bovis but slightly smaller, measuring 2.94–4.41
μm
×
2.94–4.41
μm (mean
=
3.94
μm
×
3.44
μm) with a length/width shape index of 1.15 (
n
=
25). Oocysts of
C. xioai were not infectious for BALB/c mice,
Bos taurus calves, or
Capra aegagrus hircus kids. Fragments of the SSU-rDNA, HSP-70, and actin genes were amplified by PCR, purified, and PCR products were sequenced. The new species was distinct from all other
Cryptosporidium species as demonstrated by multi-locus analysis of the 3 unlinked loci. Based on morphological, molecular and biological data, this geographically widespread parasite found in
Ovis aries is recognized as a new species and is named
C. xiaoi. |
| doi_str_mv | 10.1016/j.vetpar.2009.05.011 |
| format | Article |
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Cryptosporidium xiaoi, is described from sheep. Oocysts of
C. xiaoi, previously identified as the
Cryptosporidium bovis-like genotype or as
C. bovis from sheep in Spain, Tunisia, United Kingdom, and the United States are recorded as such in GenBank (
EU408314–
EU408317,
EU327318–
EU327320,
EF362478,
EF514234,
DQ991389, and
EF158461). Oocysts obtained from naturally infected sheep were infectious for a lamb and oocysts from that lamb were infectious for three other lambs. The prepatent period for
C. xiaoi in these four
Cryptosporidium-naïve lambs was 7–8 days and the patent period was 13–15 days. Oocysts are similar to those of
C. bovis but slightly smaller, measuring 2.94–4.41
μm
×
2.94–4.41
μm (mean
=
3.94
μm
×
3.44
μm) with a length/width shape index of 1.15 (
n
=
25). Oocysts of
C. xioai were not infectious for BALB/c mice,
Bos taurus calves, or
Capra aegagrus hircus kids. Fragments of the SSU-rDNA, HSP-70, and actin genes were amplified by PCR, purified, and PCR products were sequenced. The new species was distinct from all other
Cryptosporidium species as demonstrated by multi-locus analysis of the 3 unlinked loci. Based on morphological, molecular and biological data, this geographically widespread parasite found in
Ovis aries is recognized as a new species and is named
C. xiaoi.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2009.05.011</identifier><identifier>PMID: 19501967</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Apicomplexa ; Bos taurus ; Capra ; Cattle ; Cattle Diseases ; Cryptosporidiosis ; Cryptosporidiosis - epidemiology ; Cryptosporidiosis - parasitology ; Cryptosporidiosis - veterinary ; Cryptosporidium ; Cryptosporidium - classification ; Cryptosporidium - genetics ; Cryptosporidium - isolation & purification ; Cryptosporidium xiaoi ; Feces - parasitology ; Female ; gene banks ; Goat Diseases - parasitology ; Goats ; lambs ; Male ; Maryland - epidemiology ; Mice ; Molecular ; molecular sequence data ; New species ; nucleotide sequences ; oocysts ; Ovis aries ; Phylogeny ; polymerase chain reaction ; Prevalence ; sequence analysis ; Sheep ; sheep diseases ; Sheep Diseases - epidemiology ; Sheep Diseases - parasitology ; Species Specificity ; Taxonomy ; Transmission</subject><ispartof>Veterinary parasitology, 2009-10, Vol.164 (2), p.192-200</ispartof><rights>2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-cca1b77c00c020b1b0f64b901a3bcf959ca49e899022bf04f94d4c2f2416b853</citedby><cites>FETCH-LOGICAL-c416t-cca1b77c00c020b1b0f64b901a3bcf959ca49e899022bf04f94d4c2f2416b853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304401709003008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19501967$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fayer, Ronald</creatorcontrib><creatorcontrib>Santín, Mónica</creatorcontrib><title>Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep ( Ovis aries)</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>A new species,
Cryptosporidium xiaoi, is described from sheep. Oocysts of
C. xiaoi, previously identified as the
Cryptosporidium bovis-like genotype or as
C. bovis from sheep in Spain, Tunisia, United Kingdom, and the United States are recorded as such in GenBank (
EU408314–
EU408317,
EU327318–
EU327320,
EF362478,
EF514234,
DQ991389, and
EF158461). Oocysts obtained from naturally infected sheep were infectious for a lamb and oocysts from that lamb were infectious for three other lambs. The prepatent period for
C. xiaoi in these four
Cryptosporidium-naïve lambs was 7–8 days and the patent period was 13–15 days. Oocysts are similar to those of
C. bovis but slightly smaller, measuring 2.94–4.41
μm
×
2.94–4.41
μm (mean
=
3.94
μm
×
3.44
μm) with a length/width shape index of 1.15 (
n
=
25). Oocysts of
C. xioai were not infectious for BALB/c mice,
Bos taurus calves, or
Capra aegagrus hircus kids. Fragments of the SSU-rDNA, HSP-70, and actin genes were amplified by PCR, purified, and PCR products were sequenced. The new species was distinct from all other
Cryptosporidium species as demonstrated by multi-locus analysis of the 3 unlinked loci. Based on morphological, molecular and biological data, this geographically widespread parasite found in
Ovis aries is recognized as a new species and is named
C. xiaoi.</description><subject>Animals</subject><subject>Apicomplexa</subject><subject>Bos taurus</subject><subject>Capra</subject><subject>Cattle</subject><subject>Cattle Diseases</subject><subject>Cryptosporidiosis</subject><subject>Cryptosporidiosis - epidemiology</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - classification</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium - isolation & purification</subject><subject>Cryptosporidium xiaoi</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>gene banks</subject><subject>Goat Diseases - parasitology</subject><subject>Goats</subject><subject>lambs</subject><subject>Male</subject><subject>Maryland - epidemiology</subject><subject>Mice</subject><subject>Molecular</subject><subject>molecular sequence data</subject><subject>New species</subject><subject>nucleotide sequences</subject><subject>oocysts</subject><subject>Ovis aries</subject><subject>Phylogeny</subject><subject>polymerase chain reaction</subject><subject>Prevalence</subject><subject>sequence analysis</subject><subject>Sheep</subject><subject>sheep diseases</subject><subject>Sheep Diseases - epidemiology</subject><subject>Sheep Diseases - parasitology</subject><subject>Species Specificity</subject><subject>Taxonomy</subject><subject>Transmission</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEuLFDEURoMoTjv6D0Sz0plFlfcmqUdcCEPjCwZmYbsOqdQtTdPViUl1M_PvraYaxM2s7uZ8h8th7DVCiYD1h215pCnaVAoAXUJVAuITtsK2kYWoKnjKViBBFQqwuWAvct4CgIK6ec4uUFeAum5WbLNOD3EKOYbke38Y-b23wfN9yXMs-dVN9C6McUf39iP_D_W9pWvu9zz_Jor8it8dfeY2ecrXL9mzwe4yvTrfS7b58nmz_lbc3n39vr65LZzCeiqcs9g1jQNwIKDDDoZadRrQys4NutLOKk2t1iBEN4AatOqVE4OY111byUv2ftHGFP4cKE9m9NnRbmf3FA7ZNFJBpZRsZ_Ldo6RAaFDWJ6VaQJdCzokGE5MfbXowCOaU3WzNkt2cshuozJx9nr05-w_dSP2_0bnzDLxdgMEGY38ln83PHwJQzspWti3MxKeFoDnY0VMy2XnaO-p9IjeZPvjHf_gLV1OdoA</recordid><startdate>20091014</startdate><enddate>20091014</enddate><creator>Fayer, Ronald</creator><creator>Santín, Mónica</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20091014</creationdate><title>Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep ( Ovis aries)</title><author>Fayer, Ronald ; Santín, Mónica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-cca1b77c00c020b1b0f64b901a3bcf959ca49e899022bf04f94d4c2f2416b853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Apicomplexa</topic><topic>Bos taurus</topic><topic>Capra</topic><topic>Cattle</topic><topic>Cattle Diseases</topic><topic>Cryptosporidiosis</topic><topic>Cryptosporidiosis - epidemiology</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidiosis - veterinary</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - classification</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium - isolation & purification</topic><topic>Cryptosporidium xiaoi</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>gene banks</topic><topic>Goat Diseases - parasitology</topic><topic>Goats</topic><topic>lambs</topic><topic>Male</topic><topic>Maryland - epidemiology</topic><topic>Mice</topic><topic>Molecular</topic><topic>molecular sequence data</topic><topic>New species</topic><topic>nucleotide sequences</topic><topic>oocysts</topic><topic>Ovis aries</topic><topic>Phylogeny</topic><topic>polymerase chain reaction</topic><topic>Prevalence</topic><topic>sequence analysis</topic><topic>Sheep</topic><topic>sheep diseases</topic><topic>Sheep Diseases - epidemiology</topic><topic>Sheep Diseases - parasitology</topic><topic>Species Specificity</topic><topic>Taxonomy</topic><topic>Transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fayer, Ronald</creatorcontrib><creatorcontrib>Santín, Mónica</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fayer, Ronald</au><au>Santín, Mónica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep ( Ovis aries)</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2009-10-14</date><risdate>2009</risdate><volume>164</volume><issue>2</issue><spage>192</spage><epage>200</epage><pages>192-200</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>A new species,
Cryptosporidium xiaoi, is described from sheep. Oocysts of
C. xiaoi, previously identified as the
Cryptosporidium bovis-like genotype or as
C. bovis from sheep in Spain, Tunisia, United Kingdom, and the United States are recorded as such in GenBank (
EU408314–
EU408317,
EU327318–
EU327320,
EF362478,
EF514234,
DQ991389, and
EF158461). Oocysts obtained from naturally infected sheep were infectious for a lamb and oocysts from that lamb were infectious for three other lambs. The prepatent period for
C. xiaoi in these four
Cryptosporidium-naïve lambs was 7–8 days and the patent period was 13–15 days. Oocysts are similar to those of
C. bovis but slightly smaller, measuring 2.94–4.41
μm
×
2.94–4.41
μm (mean
=
3.94
μm
×
3.44
μm) with a length/width shape index of 1.15 (
n
=
25). Oocysts of
C. xioai were not infectious for BALB/c mice,
Bos taurus calves, or
Capra aegagrus hircus kids. Fragments of the SSU-rDNA, HSP-70, and actin genes were amplified by PCR, purified, and PCR products were sequenced. The new species was distinct from all other
Cryptosporidium species as demonstrated by multi-locus analysis of the 3 unlinked loci. Based on morphological, molecular and biological data, this geographically widespread parasite found in
Ovis aries is recognized as a new species and is named
C. xiaoi.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>19501967</pmid><doi>10.1016/j.vetpar.2009.05.011</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 2009-10, Vol.164 (2), p.192-200 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_734054438 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Apicomplexa Bos taurus Capra Cattle Cattle Diseases Cryptosporidiosis Cryptosporidiosis - epidemiology Cryptosporidiosis - parasitology Cryptosporidiosis - veterinary Cryptosporidium Cryptosporidium - classification Cryptosporidium - genetics Cryptosporidium - isolation & purification Cryptosporidium xiaoi Feces - parasitology Female gene banks Goat Diseases - parasitology Goats lambs Male Maryland - epidemiology Mice Molecular molecular sequence data New species nucleotide sequences oocysts Ovis aries Phylogeny polymerase chain reaction Prevalence sequence analysis Sheep sheep diseases Sheep Diseases - epidemiology Sheep Diseases - parasitology Species Specificity Taxonomy Transmission |
| title | Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep ( Ovis aries) |
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