Cloning and characterization of Dorsal homologues in the hemipteran Rhodnius prolixus
Rhodnius prolixus is an ancient haematophagous hemipteran insect capable of mounting a powerful immune response. This response is transcriptionally regulated in part by transcription factors of the Rel/Nuclear Factor kappa B (Rel/NF-κB) family. We have cloned and characterized three members of this...
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| Veröffentlicht in: | Insect molecular biology 2009-10, Vol.18 (5), p.681-689 |
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| creator | Ursic-Bedoya, R Buchhop, J Lowenberger, C |
| description | Rhodnius prolixus is an ancient haematophagous hemipteran insect capable of mounting a powerful immune response. This response is transcriptionally regulated in part by transcription factors of the Rel/Nuclear Factor kappa B (Rel/NF-κB) family. We have cloned and characterized three members of this transcription factor family in this insect. Dorsal 1A is primarily expressed in early developmental stages. In contrast, dorsal 1B and 1C, both differentially spliced products of dorsal 1A, are expressed primarily in the adult fat body in response to septic injury, suggesting their exclusive role in immunity. Additionally, we identified putative κB binding sites in the 5' upstream regions of target genes known to be involved in the innate immune response of insects. |
| doi_str_mv | 10.1111/j.1365-2583.2009.00909.x |
| format | Article |
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This response is transcriptionally regulated in part by transcription factors of the Rel/Nuclear Factor kappa B (Rel/NF-κB) family. We have cloned and characterized three members of this transcription factor family in this insect. Dorsal 1A is primarily expressed in early developmental stages. In contrast, dorsal 1B and 1C, both differentially spliced products of dorsal 1A, are expressed primarily in the adult fat body in response to septic injury, suggesting their exclusive role in immunity. Additionally, we identified putative κB binding sites in the 5' upstream regions of target genes known to be involved in the innate immune response of insects.</description><identifier>ISSN: 0962-1075</identifier><identifier>EISSN: 1365-2583</identifier><identifier>DOI: 10.1111/j.1365-2583.2009.00909.x</identifier><identifier>PMID: 19754745</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Animals ; antimicrobial peptides ; Base Sequence ; Binding Sites ; Cloning, Molecular ; Drosophila Proteins - chemistry ; Gene Expression Profiling ; Gene Expression Regulation ; gene regulation ; Genes, Insect ; insect immunity ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Molecular Sequence Data ; Nuclear Proteins - chemistry ; Phosphoproteins - chemistry ; Recombinant Proteins - metabolism ; Rhodnius - genetics ; Rhodnius prolixus ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Analysis, Protein ; Sequence Homology, Amino Acid ; transcription factor ; Transcription Factors - chemistry ; Transcription Factors - metabolism</subject><ispartof>Insect molecular biology, 2009-10, Vol.18 (5), p.681-689</ispartof><rights>2009 The Authors. 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This response is transcriptionally regulated in part by transcription factors of the Rel/Nuclear Factor kappa B (Rel/NF-κB) family. We have cloned and characterized three members of this transcription factor family in this insect. Dorsal 1A is primarily expressed in early developmental stages. In contrast, dorsal 1B and 1C, both differentially spliced products of dorsal 1A, are expressed primarily in the adult fat body in response to septic injury, suggesting their exclusive role in immunity. Additionally, we identified putative κB binding sites in the 5' upstream regions of target genes known to be involved in the innate immune response of insects.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>antimicrobial peptides</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Cloning, Molecular</subject><subject>Drosophila Proteins - chemistry</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>gene regulation</subject><subject>Genes, Insect</subject><subject>insect immunity</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - chemistry</subject><subject>Phosphoproteins - chemistry</subject><subject>Recombinant Proteins - metabolism</subject><subject>Rhodnius - genetics</subject><subject>Rhodnius prolixus</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Analysis, Protein</subject><subject>Sequence Homology, Amino Acid</subject><subject>transcription factor</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - metabolism</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1v0zAUhi0EYmXwF8DiAq5S7Dj-krgZHYxJ65AYhUvLSZzGJbGLnYiOX4-zVEPiAmHJPpb8vOfDLwAQoyVO681uiQmjWU4FWeYIyWXa6Tw8AIv7h4dggSTLM4w4PQFPYtwhhIRk8jE4wZLTghd0ATarzjvrtlC7GlatDroaTLC_9GC9g76B5z5E3cHW977z29FEaB0cWgNb09t9YrWDn1tfOztGuA--s4cxPgWPGt1F8-wYT8Hmw_svq4_Z1aeLy9XZVVYVgstMSqlrWha8pGXDJUEUSZL6bRjN6dSilAJxWVJaISkb1mhc5Jo3oiaaUMzIKXg9502Ff6TeBtXbWJmu0874MSpOClQIKnEiX_2TzJEgmIkJfPkXuPNjcGmKxBQCFeiurpihKvgYg2nUPtheh1uFkZocUjs1GaEmI9TkkLpzSB2S9Pkx_1j2pv4jPFqSgLcz8NN25va_E6vL9bt0SfJslts4mMO9XIfvinHCqfp2faG-snWO19fn6ibxL2a-0V7pbbBRbW5yhEmak8v0feQ3V1C1eg</recordid><startdate>200910</startdate><enddate>200910</enddate><creator>Ursic-Bedoya, R</creator><creator>Buchhop, J</creator><creator>Lowenberger, C</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200910</creationdate><title>Cloning and characterization of Dorsal homologues in the hemipteran Rhodnius prolixus</title><author>Ursic-Bedoya, R ; Buchhop, J ; Lowenberger, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4879-999ad5b47b5bf79305093107f65259754998079b55c099f6fa142a7f8d3a35163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>antimicrobial peptides</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Cloning, Molecular</topic><topic>Drosophila Proteins - chemistry</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>gene regulation</topic><topic>Genes, Insect</topic><topic>insect immunity</topic><topic>Insect Proteins - chemistry</topic><topic>Insect Proteins - genetics</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins - chemistry</topic><topic>Phosphoproteins - chemistry</topic><topic>Recombinant Proteins - metabolism</topic><topic>Rhodnius - genetics</topic><topic>Rhodnius prolixus</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Analysis, Protein</topic><topic>Sequence Homology, Amino Acid</topic><topic>transcription factor</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ursic-Bedoya, R</creatorcontrib><creatorcontrib>Buchhop, J</creatorcontrib><creatorcontrib>Lowenberger, C</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ursic-Bedoya, R</au><au>Buchhop, J</au><au>Lowenberger, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of Dorsal homologues in the hemipteran Rhodnius prolixus</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2009-10</date><risdate>2009</risdate><volume>18</volume><issue>5</issue><spage>681</spage><epage>689</epage><pages>681-689</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>Rhodnius prolixus is an ancient haematophagous hemipteran insect capable of mounting a powerful immune response. This response is transcriptionally regulated in part by transcription factors of the Rel/Nuclear Factor kappa B (Rel/NF-κB) family. We have cloned and characterized three members of this transcription factor family in this insect. Dorsal 1A is primarily expressed in early developmental stages. In contrast, dorsal 1B and 1C, both differentially spliced products of dorsal 1A, are expressed primarily in the adult fat body in response to septic injury, suggesting their exclusive role in immunity. Additionally, we identified putative κB binding sites in the 5' upstream regions of target genes known to be involved in the innate immune response of insects.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19754745</pmid><doi>10.1111/j.1365-2583.2009.00909.x</doi><tpages>9</tpages></addata></record> |
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| subjects | Amino Acid Sequence Animals antimicrobial peptides Base Sequence Binding Sites Cloning, Molecular Drosophila Proteins - chemistry Gene Expression Profiling Gene Expression Regulation gene regulation Genes, Insect insect immunity Insect Proteins - chemistry Insect Proteins - genetics Molecular Sequence Data Nuclear Proteins - chemistry Phosphoproteins - chemistry Recombinant Proteins - metabolism Rhodnius - genetics Rhodnius prolixus RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Analysis, Protein Sequence Homology, Amino Acid transcription factor Transcription Factors - chemistry Transcription Factors - metabolism |
| title | Cloning and characterization of Dorsal homologues in the hemipteran Rhodnius prolixus |
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