Comparison of enzyme immobilisation methods for potentiometric phosphate biosensors

The development of two phosphate biosensors is described and compared for potentiometric detection of phosphate. Purine nucleoside phosphorylase (PNP) and xanthine oxidase (XOD) were co-immobilised by chemical cross-linking with glutaraldehyde (GLA) and bovine serum albumin (BSA), and via entrapment...

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Veröffentlicht in:	Biosensors & bioelectronics 2009-10, Vol.25 (2), p.406-410
Hauptverfasser:	Lawal, A.T., Adeloju, S.B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biosensing Techniques - instrumentation Biosensor Biosensors Biotechnology Electrochemistry - instrumentation Electrodes Enzyme Activation Enzymes, Immobilized - chemistry Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology General aspects Immobilisation methods Immobilization techniques Methods. Procedures. Technologies Phosphate Phosphates - analysis Phosphates - chemistry Polypyrrole Potentiometric detection Purine-Nucleoside Phosphorylase - chemistry Reproducibility of Results Sensitivity and Specificity Transducers Various methods and equipments Xanthine Oxidase - chemistry
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