Myocardial IL-6 regulation by neurohormones—an in vitro superfusion study
Background: Interleukin-6 (IL-6) is expressed in the myocardium and has been implicated in cell proliferation, negative inotropic effects and myocardial hypertrophy. To determine whether myocardial IL-6 is modified by neurohumoral and immunoregulatory stimuli, we studied the effects of lipopolysacch...
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| Veröffentlicht in: | Brain, behavior, and immunity behavior, and immunity, 2003-08, Vol.17 (4), p.245-250 |
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| creator | Jeron, Andreas Kaiser, Tanja Straub, Rainer H Weil, Joachim Riegger, Günter A.J Muders, Frank |
| description | Background: Interleukin-6 (IL-6) is expressed in the myocardium and has been implicated in cell proliferation, negative inotropic effects and myocardial hypertrophy. To determine whether myocardial IL-6 is modified by neurohumoral and immunoregulatory stimuli, we studied the effects of lipopolysaccharide (LPS), corticosterone (CS), isoproterenol and angiotensin II on myocardial IL-6 secretion in superfused myocardium.
Methods: Slices of rat left ventricular myocardium were superfused in 80
μl chambers for up to 5
h. LPS (1, 50, and 100
μg/ml), CS (10
−7, 10
−6, and 10
−5
M, DSMO as vehicle), isoproterenol (10
−6, 10
−7, and 10
−8
M) and angiotensin II (10
−5, 10
−7, and 10
−9
M) were added to the culture medium at hour 2. IL-6 was measured in the perfusate by ELISA.
Results: Physiological corticosterone concentrations (10
−7
M) resulted in an increase in IL-6 concentration (142%) while high doses of steroid decreased IL-6 significantly (CS 10
−6
M:
88±14%,
p |
| doi_str_mv | 10.1016/S0889-1591(03)00053-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73404307</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0889159103000539</els_id><sourcerecordid>18941279</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-d987489335a8bac7bde40f4e361d008c1d80b0cd30523a1224631595e63a0c8b3</originalsourceid><addsrcrecordid>eNqFkMtOwzAQRS0EoqXwCaCsECwC4zgPe4VQxaOiiAWwthx7CkZpXOykUnd8BF_Il5A-BMuuZhbnztUcQo4pXFCg-eUzcC5imgl6BuwcADIWix3SpyAgTigTu6T_h_TIQQgfK4jyfdKjCe-WJO-Th8eF08obq6poNI7zyONbW6nGujoqF1GNrXfvzk9djeHn61vVka2juW28i0I7Qz9pwxINTWsWh2RvoqqAR5s5IK-3Ny_D-3j8dDcaXo9jzUTSxEbwIuWCsUzxUumiNJjCJEWWUwPANTUcStCGQZYwRZMkzVn3RIY5U6B5yQbkdH135t1ni6GRUxs0VpWq0bVBFiyFlEGxFaRcpDQpRAdma1B7F4LHiZx5O1V-ISnIpW650i2XLiUwuTIpl7mTTUFbTtH8pzZ-O-BqDWDnY27Ry6At1hqN9agbaZzdUvELPyePhg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18941279</pqid></control><display><type>article</type><title>Myocardial IL-6 regulation by neurohormones—an in vitro superfusion study</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Jeron, Andreas ; Kaiser, Tanja ; Straub, Rainer H ; Weil, Joachim ; Riegger, Günter A.J ; Muders, Frank</creator><creatorcontrib>Jeron, Andreas ; Kaiser, Tanja ; Straub, Rainer H ; Weil, Joachim ; Riegger, Günter A.J ; Muders, Frank</creatorcontrib><description>Background: Interleukin-6 (IL-6) is expressed in the myocardium and has been implicated in cell proliferation, negative inotropic effects and myocardial hypertrophy. To determine whether myocardial IL-6 is modified by neurohumoral and immunoregulatory stimuli, we studied the effects of lipopolysaccharide (LPS), corticosterone (CS), isoproterenol and angiotensin II on myocardial IL-6 secretion in superfused myocardium.
Methods: Slices of rat left ventricular myocardium were superfused in 80
μl chambers for up to 5
h. LPS (1, 50, and 100
μg/ml), CS (10
−7, 10
−6, and 10
−5
M, DSMO as vehicle), isoproterenol (10
−6, 10
−7, and 10
−8
M) and angiotensin II (10
−5, 10
−7, and 10
−9
M) were added to the culture medium at hour 2. IL-6 was measured in the perfusate by ELISA.
Results: Physiological corticosterone concentrations (10
−7
M) resulted in an increase in IL-6 concentration (142%) while high doses of steroid decreased IL-6 significantly (CS 10
−6
M:
88±14%,
p<.05
; CS 10
−5:
91±9%,
p<.05
) after 5
h. Left ventricular IL-6 secretion was significantly stimulated by LPS 50
μg/ml (3262±1684% vs. CTRL: 116±34%,
p<.01). Isoproterenol treatment increased in IL-6 secretion compared to controls with and without CS, while angiotensin II reduced IL-6 concentration only in combination with CS.
Conclusion: Myocardial IL-6 secretion is modulated by physiological concentrations of corticosterone or angiotensin II and can be induced by LPS or isoproterenol, indicating a tight regulation of this cytokine. Suppression of cytokine expression within the heart might be a potential therapeutic goal in the treatment of various cardiovascular diseases.</description><identifier>ISSN: 0889-1591</identifier><identifier>EISSN: 1090-2139</identifier><identifier>DOI: 10.1016/S0889-1591(03)00053-9</identifier><identifier>PMID: 12831826</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>angiotensin II ; Angiotensin II - physiology ; Animals ; Cardiotonic Agents - pharmacology ; Corticosterone - physiology ; Cytokines ; Dose-Response Relationship, Drug ; IL-6 ; Immunoregulation ; Interleukin-6 - secretion ; isoproterenol ; Isoproterenol - pharmacology ; Lipopolysaccharides - pharmacology ; Male ; Myocardium ; Myocardium - metabolism ; Organ Culture Techniques ; Perfusion ; Rats ; Rats, Wistar ; Superfusion</subject><ispartof>Brain, behavior, and immunity, 2003-08, Vol.17 (4), p.245-250</ispartof><rights>2003 Elsevier Science (USA)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-d987489335a8bac7bde40f4e361d008c1d80b0cd30523a1224631595e63a0c8b3</citedby><cites>FETCH-LOGICAL-c392t-d987489335a8bac7bde40f4e361d008c1d80b0cd30523a1224631595e63a0c8b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0889-1591(03)00053-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12831826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jeron, Andreas</creatorcontrib><creatorcontrib>Kaiser, Tanja</creatorcontrib><creatorcontrib>Straub, Rainer H</creatorcontrib><creatorcontrib>Weil, Joachim</creatorcontrib><creatorcontrib>Riegger, Günter A.J</creatorcontrib><creatorcontrib>Muders, Frank</creatorcontrib><title>Myocardial IL-6 regulation by neurohormones—an in vitro superfusion study</title><title>Brain, behavior, and immunity</title><addtitle>Brain Behav Immun</addtitle><description>Background: Interleukin-6 (IL-6) is expressed in the myocardium and has been implicated in cell proliferation, negative inotropic effects and myocardial hypertrophy. To determine whether myocardial IL-6 is modified by neurohumoral and immunoregulatory stimuli, we studied the effects of lipopolysaccharide (LPS), corticosterone (CS), isoproterenol and angiotensin II on myocardial IL-6 secretion in superfused myocardium.
Methods: Slices of rat left ventricular myocardium were superfused in 80
μl chambers for up to 5
h. LPS (1, 50, and 100
μg/ml), CS (10
−7, 10
−6, and 10
−5
M, DSMO as vehicle), isoproterenol (10
−6, 10
−7, and 10
−8
M) and angiotensin II (10
−5, 10
−7, and 10
−9
M) were added to the culture medium at hour 2. IL-6 was measured in the perfusate by ELISA.
Results: Physiological corticosterone concentrations (10
−7
M) resulted in an increase in IL-6 concentration (142%) while high doses of steroid decreased IL-6 significantly (CS 10
−6
M:
88±14%,
p<.05
; CS 10
−5:
91±9%,
p<.05
) after 5
h. Left ventricular IL-6 secretion was significantly stimulated by LPS 50
μg/ml (3262±1684% vs. CTRL: 116±34%,
p<.01). Isoproterenol treatment increased in IL-6 secretion compared to controls with and without CS, while angiotensin II reduced IL-6 concentration only in combination with CS.
Conclusion: Myocardial IL-6 secretion is modulated by physiological concentrations of corticosterone or angiotensin II and can be induced by LPS or isoproterenol, indicating a tight regulation of this cytokine. Suppression of cytokine expression within the heart might be a potential therapeutic goal in the treatment of various cardiovascular diseases.</description><subject>angiotensin II</subject><subject>Angiotensin II - physiology</subject><subject>Animals</subject><subject>Cardiotonic Agents - pharmacology</subject><subject>Corticosterone - physiology</subject><subject>Cytokines</subject><subject>Dose-Response Relationship, Drug</subject><subject>IL-6</subject><subject>Immunoregulation</subject><subject>Interleukin-6 - secretion</subject><subject>isoproterenol</subject><subject>Isoproterenol - pharmacology</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>Myocardium</subject><subject>Myocardium - metabolism</subject><subject>Organ Culture Techniques</subject><subject>Perfusion</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Superfusion</subject><issn>0889-1591</issn><issn>1090-2139</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EoqXwCaCsECwC4zgPe4VQxaOiiAWwthx7CkZpXOykUnd8BF_Il5A-BMuuZhbnztUcQo4pXFCg-eUzcC5imgl6BuwcADIWix3SpyAgTigTu6T_h_TIQQgfK4jyfdKjCe-WJO-Th8eF08obq6poNI7zyONbW6nGujoqF1GNrXfvzk9djeHn61vVka2juW28i0I7Qz9pwxINTWsWh2RvoqqAR5s5IK-3Ny_D-3j8dDcaXo9jzUTSxEbwIuWCsUzxUumiNJjCJEWWUwPANTUcStCGQZYwRZMkzVn3RIY5U6B5yQbkdH135t1ni6GRUxs0VpWq0bVBFiyFlEGxFaRcpDQpRAdma1B7F4LHiZx5O1V-ISnIpW650i2XLiUwuTIpl7mTTUFbTtH8pzZ-O-BqDWDnY27Ry6At1hqN9agbaZzdUvELPyePhg</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Jeron, Andreas</creator><creator>Kaiser, Tanja</creator><creator>Straub, Rainer H</creator><creator>Weil, Joachim</creator><creator>Riegger, Günter A.J</creator><creator>Muders, Frank</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>Myocardial IL-6 regulation by neurohormones—an in vitro superfusion study</title><author>Jeron, Andreas ; Kaiser, Tanja ; Straub, Rainer H ; Weil, Joachim ; Riegger, Günter A.J ; Muders, Frank</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-d987489335a8bac7bde40f4e361d008c1d80b0cd30523a1224631595e63a0c8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>angiotensin II</topic><topic>Angiotensin II - physiology</topic><topic>Animals</topic><topic>Cardiotonic Agents - pharmacology</topic><topic>Corticosterone - physiology</topic><topic>Cytokines</topic><topic>Dose-Response Relationship, Drug</topic><topic>IL-6</topic><topic>Immunoregulation</topic><topic>Interleukin-6 - secretion</topic><topic>isoproterenol</topic><topic>Isoproterenol - pharmacology</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>Myocardium</topic><topic>Myocardium - metabolism</topic><topic>Organ Culture Techniques</topic><topic>Perfusion</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Superfusion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jeron, Andreas</creatorcontrib><creatorcontrib>Kaiser, Tanja</creatorcontrib><creatorcontrib>Straub, Rainer H</creatorcontrib><creatorcontrib>Weil, Joachim</creatorcontrib><creatorcontrib>Riegger, Günter A.J</creatorcontrib><creatorcontrib>Muders, Frank</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain, behavior, and immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jeron, Andreas</au><au>Kaiser, Tanja</au><au>Straub, Rainer H</au><au>Weil, Joachim</au><au>Riegger, Günter A.J</au><au>Muders, Frank</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Myocardial IL-6 regulation by neurohormones—an in vitro superfusion study</atitle><jtitle>Brain, behavior, and immunity</jtitle><addtitle>Brain Behav Immun</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>17</volume><issue>4</issue><spage>245</spage><epage>250</epage><pages>245-250</pages><issn>0889-1591</issn><eissn>1090-2139</eissn><abstract>Background: Interleukin-6 (IL-6) is expressed in the myocardium and has been implicated in cell proliferation, negative inotropic effects and myocardial hypertrophy. To determine whether myocardial IL-6 is modified by neurohumoral and immunoregulatory stimuli, we studied the effects of lipopolysaccharide (LPS), corticosterone (CS), isoproterenol and angiotensin II on myocardial IL-6 secretion in superfused myocardium.
Methods: Slices of rat left ventricular myocardium were superfused in 80
μl chambers for up to 5
h. LPS (1, 50, and 100
μg/ml), CS (10
−7, 10
−6, and 10
−5
M, DSMO as vehicle), isoproterenol (10
−6, 10
−7, and 10
−8
M) and angiotensin II (10
−5, 10
−7, and 10
−9
M) were added to the culture medium at hour 2. IL-6 was measured in the perfusate by ELISA.
Results: Physiological corticosterone concentrations (10
−7
M) resulted in an increase in IL-6 concentration (142%) while high doses of steroid decreased IL-6 significantly (CS 10
−6
M:
88±14%,
p<.05
; CS 10
−5:
91±9%,
p<.05
) after 5
h. Left ventricular IL-6 secretion was significantly stimulated by LPS 50
μg/ml (3262±1684% vs. CTRL: 116±34%,
p<.01). Isoproterenol treatment increased in IL-6 secretion compared to controls with and without CS, while angiotensin II reduced IL-6 concentration only in combination with CS.
Conclusion: Myocardial IL-6 secretion is modulated by physiological concentrations of corticosterone or angiotensin II and can be induced by LPS or isoproterenol, indicating a tight regulation of this cytokine. Suppression of cytokine expression within the heart might be a potential therapeutic goal in the treatment of various cardiovascular diseases.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>12831826</pmid><doi>10.1016/S0889-1591(03)00053-9</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0889-1591 |
| ispartof | Brain, behavior, and immunity, 2003-08, Vol.17 (4), p.245-250 |
| issn | 0889-1591 1090-2139 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73404307 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | angiotensin II Angiotensin II - physiology Animals Cardiotonic Agents - pharmacology Corticosterone - physiology Cytokines Dose-Response Relationship, Drug IL-6 Immunoregulation Interleukin-6 - secretion isoproterenol Isoproterenol - pharmacology Lipopolysaccharides - pharmacology Male Myocardium Myocardium - metabolism Organ Culture Techniques Perfusion Rats Rats, Wistar Superfusion |
| title | Myocardial IL-6 regulation by neurohormones—an in vitro superfusion study |
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