Rapid detection of resistant tuberculosis by nitrate reductase assay performed in three settings in Brazil
Objectives To evaluate nitrate reductase assay (NRA) efficacy for streptomycin, isoniazid, rifampicin and ethambutol susceptibility testing of Mycobacterium tuberculosis strains. Methods Results were generated by three laboratories: the Instituto Adolfo Lutz (IAL) Mycobacteria Reference Laboratory a...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2009-10, Vol.64 (4), p.794-796 |
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| creator | Shikama, Maria de Lourdes Silva, Regina Ruivo Ferro E. Martins, Maria Conceição Giampaglia, Carmen Maria Saraiva Oliveira, Rosângela Siqueira Silva, Rosmari F. A. M. Silva, Paula Ferro E. da Silva Telles, Maria Alice Martin, Anandi Palomino, Juan Carlos |
| description | Objectives To evaluate nitrate reductase assay (NRA) efficacy for streptomycin, isoniazid, rifampicin and ethambutol susceptibility testing of Mycobacterium tuberculosis strains. Methods Results were generated by three laboratories: the Instituto Adolfo Lutz (IAL) Mycobacteria Reference Laboratory and two IAL Regional Laboratories in Santo André and Sorocaba, São Paulo State, Brazil. One hundred and twenty M. tuberculosis strains were simultaneously tested using NRA and the proportion method (PM), while 117 strains were tested using both NRA and BACTEC MGIT 960 (M960). Results Repeatability analysis of NRA results showed rates of 100% for isoniazid and ethambutol and 97% for streptomycin and rifampicin susceptibility detection, representing substantial agreement. McNemar testing of the data also indicates that NRA and PM, as well as NRA and M960, do not differ significantly. On average, NRA results were available after 10 days. Conclusions The data demonstrate that NRA is reliable for susceptibility testing of isoniazid and rifampicin, the two most important drugs for the treatment of tuberculosis. In addition, the reduction in the time necessary to obtain susceptibility results is of fundamental importance. |
| doi_str_mv | 10.1093/jac/dkp284 |
| format | Article |
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A. M. ; Silva, Paula Ferro E. ; da Silva Telles, Maria Alice ; Martin, Anandi ; Palomino, Juan Carlos</creator><creatorcontrib>Shikama, Maria de Lourdes ; Silva, Regina Ruivo Ferro E. ; Martins, Maria Conceição ; Giampaglia, Carmen Maria Saraiva ; Oliveira, Rosângela Siqueira ; Silva, Rosmari F. A. M. ; Silva, Paula Ferro E. ; da Silva Telles, Maria Alice ; Martin, Anandi ; Palomino, Juan Carlos</creatorcontrib><description>Objectives To evaluate nitrate reductase assay (NRA) efficacy for streptomycin, isoniazid, rifampicin and ethambutol susceptibility testing of Mycobacterium tuberculosis strains. Methods Results were generated by three laboratories: the Instituto Adolfo Lutz (IAL) Mycobacteria Reference Laboratory and two IAL Regional Laboratories in Santo André and Sorocaba, São Paulo State, Brazil. One hundred and twenty M. tuberculosis strains were simultaneously tested using NRA and the proportion method (PM), while 117 strains were tested using both NRA and BACTEC MGIT 960 (M960). Results Repeatability analysis of NRA results showed rates of 100% for isoniazid and ethambutol and 97% for streptomycin and rifampicin susceptibility detection, representing substantial agreement. McNemar testing of the data also indicates that NRA and PM, as well as NRA and M960, do not differ significantly. On average, NRA results were available after 10 days. Conclusions The data demonstrate that NRA is reliable for susceptibility testing of isoniazid and rifampicin, the two most important drugs for the treatment of tuberculosis. In addition, the reduction in the time necessary to obtain susceptibility results is of fundamental importance.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkp284</identifier><identifier>PMID: 19671589</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Antibiotics ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antitubercular Agents - pharmacology ; Bacterial diseases ; Biological and medical sciences ; Brazil ; Comparative studies ; Drug resistance ; drug susceptibility testing ; Human bacterial diseases ; Humans ; Infectious diseases ; M. tuberculosis diagnostic ; Medical sciences ; MGIT 960 ; Microbial Sensitivity Tests - methods ; Mycobacterium tuberculosis - drug effects ; Mycobacterium tuberculosis - enzymology ; Nitrate Reductase - metabolism ; NRA ; Pharmacology. Drug treatments ; proportion method ; Public health ; Reproducibility of Results ; Time Factors ; Tuberculosis ; Tuberculosis and atypical mycobacterial infections ; Tuberculosis, Multidrug-Resistant - diagnosis ; Tuberculosis, Multidrug-Resistant - microbiology</subject><ispartof>Journal of antimicrobial chemotherapy, 2009-10, Vol.64 (4), p.794-796</ispartof><rights>The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org 2009</rights><rights>2009 INIST-CNRS</rights><rights>The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-32a3b038c32089778737fd4cabd7c72bac4c2271ed18b40fdcc68005c01ac4ba3</citedby><cites>FETCH-LOGICAL-c447t-32a3b038c32089778737fd4cabd7c72bac4c2271ed18b40fdcc68005c01ac4ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21960719$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19671589$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shikama, Maria de Lourdes</creatorcontrib><creatorcontrib>Silva, Regina Ruivo Ferro E.</creatorcontrib><creatorcontrib>Martins, Maria Conceição</creatorcontrib><creatorcontrib>Giampaglia, Carmen Maria Saraiva</creatorcontrib><creatorcontrib>Oliveira, Rosângela Siqueira</creatorcontrib><creatorcontrib>Silva, Rosmari F. A. M.</creatorcontrib><creatorcontrib>Silva, Paula Ferro E.</creatorcontrib><creatorcontrib>da Silva Telles, Maria Alice</creatorcontrib><creatorcontrib>Martin, Anandi</creatorcontrib><creatorcontrib>Palomino, Juan Carlos</creatorcontrib><title>Rapid detection of resistant tuberculosis by nitrate reductase assay performed in three settings in Brazil</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Objectives To evaluate nitrate reductase assay (NRA) efficacy for streptomycin, isoniazid, rifampicin and ethambutol susceptibility testing of Mycobacterium tuberculosis strains. Methods Results were generated by three laboratories: the Instituto Adolfo Lutz (IAL) Mycobacteria Reference Laboratory and two IAL Regional Laboratories in Santo André and Sorocaba, São Paulo State, Brazil. One hundred and twenty M. tuberculosis strains were simultaneously tested using NRA and the proportion method (PM), while 117 strains were tested using both NRA and BACTEC MGIT 960 (M960). Results Repeatability analysis of NRA results showed rates of 100% for isoniazid and ethambutol and 97% for streptomycin and rifampicin susceptibility detection, representing substantial agreement. McNemar testing of the data also indicates that NRA and PM, as well as NRA and M960, do not differ significantly. On average, NRA results were available after 10 days. Conclusions The data demonstrate that NRA is reliable for susceptibility testing of isoniazid and rifampicin, the two most important drugs for the treatment of tuberculosis. In addition, the reduction in the time necessary to obtain susceptibility results is of fundamental importance.</description><subject>Antibiotics</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antitubercular Agents - pharmacology</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Brazil</subject><subject>Comparative studies</subject><subject>Drug resistance</subject><subject>drug susceptibility testing</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>M. tuberculosis diagnostic</subject><subject>Medical sciences</subject><subject>MGIT 960</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - enzymology</subject><subject>Nitrate Reductase - metabolism</subject><subject>NRA</subject><subject>Pharmacology. Drug treatments</subject><subject>proportion method</subject><subject>Public health</subject><subject>Reproducibility of Results</subject><subject>Time Factors</subject><subject>Tuberculosis</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Tuberculosis, Multidrug-Resistant - diagnosis</subject><subject>Tuberculosis, Multidrug-Resistant - microbiology</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90V1rFDEUgOEgit1Wb_wBEgQpCGNPPmaSuaxFrXRV8APEm3AmyWi2szNjkgHXX2_KLlW88Cpw8pCEN4Q8YvCcQSvONmjP3PXMtbxDVkw2UHFo2V2yAgF1pWQtjshxShsAaOpG3ydHrG0Uq3W7IpsPOAdHnc_e5jCNdOpp9CmkjGOmeel8tMswlQHtdnQMOWL2RbjFZkyeYkq4o7OP_RS33tEw0vw9ek-TzzmM39LN5EXEX2F4QO71OCT_8LCekM-vXn66uKzW71-_uThfV1ZKlSvBUXQgtBUcdKuUVkL1TlrsnLKKd2il5Vwx75juJPTO2kYD1BZY2epQnJDT_blznH4sPmWzDcn6YcDRT0sySkiQXDBZ5JN_5GZa4lgeZzhTJZFuoaBne2TjlFL0vZlj2GLcGQbmpr8p_c2-f8GPDycuXcnxhx6CF_D0ADBZHPqIow3p1vECQbG_3LTM_7-w2rvyY_7nrcR4bZoSrjaXX74aeXUl36p3a_NR_AaSF6tZ</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Shikama, Maria de Lourdes</creator><creator>Silva, Regina Ruivo Ferro E.</creator><creator>Martins, Maria Conceição</creator><creator>Giampaglia, Carmen Maria Saraiva</creator><creator>Oliveira, Rosângela Siqueira</creator><creator>Silva, Rosmari F. 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M.</creator><creator>Silva, Paula Ferro E.</creator><creator>da Silva Telles, Maria Alice</creator><creator>Martin, Anandi</creator><creator>Palomino, Juan Carlos</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Rapid detection of resistant tuberculosis by nitrate reductase assay performed in three settings in Brazil</title><author>Shikama, Maria de Lourdes ; Silva, Regina Ruivo Ferro E. ; Martins, Maria Conceição ; Giampaglia, Carmen Maria Saraiva ; Oliveira, Rosângela Siqueira ; Silva, Rosmari F. 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Drug treatments</topic><topic>proportion method</topic><topic>Public health</topic><topic>Reproducibility of Results</topic><topic>Time Factors</topic><topic>Tuberculosis</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><topic>Tuberculosis, Multidrug-Resistant - diagnosis</topic><topic>Tuberculosis, Multidrug-Resistant - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shikama, Maria de Lourdes</creatorcontrib><creatorcontrib>Silva, Regina Ruivo Ferro E.</creatorcontrib><creatorcontrib>Martins, Maria Conceição</creatorcontrib><creatorcontrib>Giampaglia, Carmen Maria Saraiva</creatorcontrib><creatorcontrib>Oliveira, Rosângela Siqueira</creatorcontrib><creatorcontrib>Silva, Rosmari F. A. 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A. M.</au><au>Silva, Paula Ferro E.</au><au>da Silva Telles, Maria Alice</au><au>Martin, Anandi</au><au>Palomino, Juan Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of resistant tuberculosis by nitrate reductase assay performed in three settings in Brazil</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>64</volume><issue>4</issue><spage>794</spage><epage>796</epage><pages>794-796</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Objectives To evaluate nitrate reductase assay (NRA) efficacy for streptomycin, isoniazid, rifampicin and ethambutol susceptibility testing of Mycobacterium tuberculosis strains. Methods Results were generated by three laboratories: the Instituto Adolfo Lutz (IAL) Mycobacteria Reference Laboratory and two IAL Regional Laboratories in Santo André and Sorocaba, São Paulo State, Brazil. One hundred and twenty M. tuberculosis strains were simultaneously tested using NRA and the proportion method (PM), while 117 strains were tested using both NRA and BACTEC MGIT 960 (M960). Results Repeatability analysis of NRA results showed rates of 100% for isoniazid and ethambutol and 97% for streptomycin and rifampicin susceptibility detection, representing substantial agreement. McNemar testing of the data also indicates that NRA and PM, as well as NRA and M960, do not differ significantly. On average, NRA results were available after 10 days. Conclusions The data demonstrate that NRA is reliable for susceptibility testing of isoniazid and rifampicin, the two most important drugs for the treatment of tuberculosis. In addition, the reduction in the time necessary to obtain susceptibility results is of fundamental importance.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>19671589</pmid><doi>10.1093/jac/dkp284</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibiotics Antibiotics. Antiinfectious agents. Antiparasitic agents Antitubercular Agents - pharmacology Bacterial diseases Biological and medical sciences Brazil Comparative studies Drug resistance drug susceptibility testing Human bacterial diseases Humans Infectious diseases M. tuberculosis diagnostic Medical sciences MGIT 960 Microbial Sensitivity Tests - methods Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - enzymology Nitrate Reductase - metabolism NRA Pharmacology. Drug treatments proportion method Public health Reproducibility of Results Time Factors Tuberculosis Tuberculosis and atypical mycobacterial infections Tuberculosis, Multidrug-Resistant - diagnosis Tuberculosis, Multidrug-Resistant - microbiology |
| title | Rapid detection of resistant tuberculosis by nitrate reductase assay performed in three settings in Brazil |
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