Forensic Imaging-Guided Recovery of Nuclear DNA from the Spinal Cord
: Our objective is to document the recovery of DNA from the spinal cord or surrounding dura mater in 11 cases of severely burned human remains. Radiographs established that portions of charred tissue contained spine segments. Multidetector computed tomography (MDCT) revealed that each spine specime...
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| Veröffentlicht in: | Journal of forensic sciences 2009-09, Vol.54 (5), p.1123-1126 |
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| container_title | Journal of forensic sciences |
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| creator | Theodore Harcke, H. Monaghan, Timothy Yee, Nicole Finelli, Louis |
| description | : Our objective is to document the recovery of DNA from the spinal cord or surrounding dura mater in 11 cases of severely burned human remains. Radiographs established that portions of charred tissue contained spine segments. Multidetector computed tomography (MDCT) revealed that each spine specimen contained an intact spinal cord remnant. A full DNA profile was obtained from seven specimens using spinal cord dura mater in six specimens and spinal cord medulla in one specimen. A partial profile was obtained from four specimens (spinal cord dura mater, 2; spinal cord medulla, 2). Bone and muscle surrounding the spinal cord appear to insulate nucleic acid containing tissue from critical thermal degradation. The spinal cord, which is easily identified by MDCT examination of remains and easily recovered at the postmortem examination, can be a source of DNA with extraction yields comparable with other tissue sources. Specimens of dura mater are preferable as processing time is faster than bone. |
| doi_str_mv | 10.1111/j.1556-4029.2009.01114.x |
| format | Article |
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Radiographs established that portions of charred tissue contained spine segments. Multidetector computed tomography (MDCT) revealed that each spine specimen contained an intact spinal cord remnant. A full DNA profile was obtained from seven specimens using spinal cord dura mater in six specimens and spinal cord medulla in one specimen. A partial profile was obtained from four specimens (spinal cord dura mater, 2; spinal cord medulla, 2). Bone and muscle surrounding the spinal cord appear to insulate nucleic acid containing tissue from critical thermal degradation. The spinal cord, which is easily identified by MDCT examination of remains and easily recovered at the postmortem examination, can be a source of DNA with extraction yields comparable with other tissue sources. 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No claim to original U.S. government works</rights><rights>Copyright American Society for Testing and Materials Sep 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4644-8da55e498ae0bd69e41334bcea228e70aec62451b1c5c17566710b10e3299ead3</citedby><cites>FETCH-LOGICAL-c4644-8da55e498ae0bd69e41334bcea228e70aec62451b1c5c17566710b10e3299ead3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1556-4029.2009.01114.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1556-4029.2009.01114.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19686394$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Theodore Harcke, H.</creatorcontrib><creatorcontrib>Monaghan, Timothy</creatorcontrib><creatorcontrib>Yee, Nicole</creatorcontrib><creatorcontrib>Finelli, Louis</creatorcontrib><title>Forensic Imaging-Guided Recovery of Nuclear DNA from the Spinal Cord</title><title>Journal of forensic sciences</title><addtitle>J Forensic Sci</addtitle><description>: Our objective is to document the recovery of DNA from the spinal cord or surrounding dura mater in 11 cases of severely burned human remains. Radiographs established that portions of charred tissue contained spine segments. Multidetector computed tomography (MDCT) revealed that each spine specimen contained an intact spinal cord remnant. A full DNA profile was obtained from seven specimens using spinal cord dura mater in six specimens and spinal cord medulla in one specimen. A partial profile was obtained from four specimens (spinal cord dura mater, 2; spinal cord medulla, 2). Bone and muscle surrounding the spinal cord appear to insulate nucleic acid containing tissue from critical thermal degradation. The spinal cord, which is easily identified by MDCT examination of remains and easily recovered at the postmortem examination, can be a source of DNA with extraction yields comparable with other tissue sources. Specimens of dura mater are preferable as processing time is faster than bone.</description><subject>Bones</subject><subject>burns</subject><subject>Burns - pathology</subject><subject>computed tomography</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - isolation & purification</subject><subject>DNA Fingerprinting - methods</subject><subject>Dura Mater - pathology</subject><subject>forensic imaging</subject><subject>Forensic medicine</subject><subject>Forensic Pathology</subject><subject>forensic science</subject><subject>Human remains</subject><subject>Humans</subject><subject>nuclear DNA</subject><subject>Spinal cord</subject><subject>Spinal Cord - pathology</subject><subject>Tomography</subject><subject>Tomography, X-Ray Computed - methods</subject><subject>virtual autopsy</subject><issn>0022-1198</issn><issn>1556-4029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1uEzEUhS0EomnhFZDFAlYz-H_sBYsqJaEoSiUoYml5PDdlwkwc7AxN3h5PExWJBcKba_l-50g-ByFMSUnzebcuqZSqEISZkhFiSpJfRbl_giaPi6doQghjBaVGn6HzlNaEEEUVfY7OqFFacSMm6GoWImxS6_F17-7azV0xH9oGGvwZfPgF8YDDCi8H34GL-Gp5iVcx9Hj3HfCXbbtxHZ6G2LxAz1auS_DyNC_Q19mH2-nHYnEzv55eLgovlBCFbpyUIIx2QOpGGRCUc1F7cIxpqIgDr5iQtKZeelpJpSpKakqAM2PANfwCvT36bmP4OUDa2b5NHrrObSAMyVZcEK4Nl5l880-SEc2ZfgBf_wWuwxDzxzKTUzKcGpEhfYR8DClFWNltbHsXD5YSOxZi13bM3Y6527EQ-1CI3Wfpq5P_UPfQ_BGeGsjA-yNw33Zw-G9j-2l2M96yvjjq27SD_aPexR9WVbyS9ttynmE6u12IhWX8N_EYpVA</recordid><startdate>200909</startdate><enddate>200909</enddate><creator>Theodore Harcke, H.</creator><creator>Monaghan, Timothy</creator><creator>Yee, Nicole</creator><creator>Finelli, Louis</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K7.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>200909</creationdate><title>Forensic Imaging-Guided Recovery of Nuclear DNA from the Spinal Cord</title><author>Theodore Harcke, H. ; Monaghan, Timothy ; Yee, Nicole ; Finelli, Louis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4644-8da55e498ae0bd69e41334bcea228e70aec62451b1c5c17566710b10e3299ead3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Bones</topic><topic>burns</topic><topic>Burns - pathology</topic><topic>computed tomography</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - isolation & purification</topic><topic>DNA Fingerprinting - methods</topic><topic>Dura Mater - pathology</topic><topic>forensic imaging</topic><topic>Forensic medicine</topic><topic>Forensic Pathology</topic><topic>forensic science</topic><topic>Human remains</topic><topic>Humans</topic><topic>nuclear DNA</topic><topic>Spinal cord</topic><topic>Spinal Cord - pathology</topic><topic>Tomography</topic><topic>Tomography, X-Ray Computed - methods</topic><topic>virtual autopsy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Theodore Harcke, H.</creatorcontrib><creatorcontrib>Monaghan, Timothy</creatorcontrib><creatorcontrib>Yee, Nicole</creatorcontrib><creatorcontrib>Finelli, Louis</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Theodore Harcke, H.</au><au>Monaghan, Timothy</au><au>Yee, Nicole</au><au>Finelli, Louis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Forensic Imaging-Guided Recovery of Nuclear DNA from the Spinal Cord</atitle><jtitle>Journal of forensic sciences</jtitle><addtitle>J Forensic Sci</addtitle><date>2009-09</date><risdate>2009</risdate><volume>54</volume><issue>5</issue><spage>1123</spage><epage>1126</epage><pages>1123-1126</pages><issn>0022-1198</issn><eissn>1556-4029</eissn><coden>JFSCAS</coden><abstract>: Our objective is to document the recovery of DNA from the spinal cord or surrounding dura mater in 11 cases of severely burned human remains. Radiographs established that portions of charred tissue contained spine segments. Multidetector computed tomography (MDCT) revealed that each spine specimen contained an intact spinal cord remnant. A full DNA profile was obtained from seven specimens using spinal cord dura mater in six specimens and spinal cord medulla in one specimen. A partial profile was obtained from four specimens (spinal cord dura mater, 2; spinal cord medulla, 2). Bone and muscle surrounding the spinal cord appear to insulate nucleic acid containing tissue from critical thermal degradation. The spinal cord, which is easily identified by MDCT examination of remains and easily recovered at the postmortem examination, can be a source of DNA with extraction yields comparable with other tissue sources. Specimens of dura mater are preferable as processing time is faster than bone.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19686394</pmid><doi>10.1111/j.1556-4029.2009.01114.x</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of forensic sciences, 2009-09, Vol.54 (5), p.1123-1126 |
| issn | 0022-1198 1556-4029 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Bones burns Burns - pathology computed tomography Deoxyribonucleic acid DNA DNA - isolation & purification DNA Fingerprinting - methods Dura Mater - pathology forensic imaging Forensic medicine Forensic Pathology forensic science Human remains Humans nuclear DNA Spinal cord Spinal Cord - pathology Tomography Tomography, X-Ray Computed - methods virtual autopsy |
| title | Forensic Imaging-Guided Recovery of Nuclear DNA from the Spinal Cord |
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