Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA
We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that tet (M) and tet (W) are the most prevalent tetracycline...
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| Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2009-09, Vol.15 (3), p.159-166 |
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| creator | Seville, Lorna A. Patterson, Andrea J. Scott, Karen P. Mullany, Peter Quail, Michael A. Parkhill, Julian Ready, Derren Wilson, Michael Spratt, David Roberts, Adam P. |
| description | We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that
tet
(M) and
tet
(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally,
tet
(Q),
tet
(O), and
tet
(O/32/O) have been shown to be common. We have also shown that
erm
(B),
erm
(V), and
erm
(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn
916
integrase in the oral metagenomes and the Tn
4451
and Tn
1549
integrase genes within the fecal metagenomes. |
| doi_str_mv | 10.1089/mdr.2009.0916 |
| format | Article |
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tet
(M) and
tet
(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally,
tet
(Q),
tet
(O), and
tet
(O/32/O) have been shown to be common. We have also shown that
erm
(B),
erm
(V), and
erm
(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn
916
integrase in the oral metagenomes and the Tn
4451
and Tn
1549
integrase genes within the fecal metagenomes.</description><identifier>ISSN: 1076-6294</identifier><identifier>EISSN: 1931-8448</identifier><identifier>DOI: 10.1089/mdr.2009.0916</identifier><identifier>PMID: 19728772</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Adult ; Aged ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Bacteria ; Bacteria - drug effects ; Bacteria - genetics ; Deoxyribonucleic acid ; DNA ; DNA Transposable Elements ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; Drug resistance ; Drug resistance in microorganisms ; Drug Resistance, Bacterial - genetics ; Erythromycin - pharmacology ; Feces - chemistry ; Feces - microbiology ; Female ; Genetic aspects ; Genome, Bacterial ; Genomics ; Health aspects ; Humans ; Male ; Mechanisms ; Middle Aged ; Molecular biology ; Oligonucleotide Array Sequence Analysis ; Saliva - chemistry ; Saliva - microbiology ; Tetracycline Resistance - genetics ; Young Adult</subject><ispartof>Microbial drug resistance (Larchmont, N.Y.), 2009-09, Vol.15 (3), p.159-166</ispartof><rights>2009, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2009 Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2009, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-4bbc533cffee2949c6ff6bd6dd03222cbd26d49880100ef3db550a2ec2ce68c33</citedby><cites>FETCH-LOGICAL-c527t-4bbc533cffee2949c6ff6bd6dd03222cbd26d49880100ef3db550a2ec2ce68c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19728772$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seville, Lorna A.</creatorcontrib><creatorcontrib>Patterson, Andrea J.</creatorcontrib><creatorcontrib>Scott, Karen P.</creatorcontrib><creatorcontrib>Mullany, Peter</creatorcontrib><creatorcontrib>Quail, Michael A.</creatorcontrib><creatorcontrib>Parkhill, Julian</creatorcontrib><creatorcontrib>Ready, Derren</creatorcontrib><creatorcontrib>Wilson, Michael</creatorcontrib><creatorcontrib>Spratt, David</creatorcontrib><creatorcontrib>Roberts, Adam P.</creatorcontrib><title>Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA</title><title>Microbial drug resistance (Larchmont, N.Y.)</title><addtitle>Microb Drug Resist</addtitle><description>We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that
tet
(M) and
tet
(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally,
tet
(Q),
tet
(O), and
tet
(O/32/O) have been shown to be common. We have also shown that
erm
(B),
erm
(V), and
erm
(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn
916
integrase in the oral metagenomes and the Tn
4451
and Tn
1549
integrase genes within the fecal metagenomes.</description><subject>Adult</subject><subject>Aged</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug resistance</subject><subject>Drug resistance in microorganisms</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Erythromycin - pharmacology</subject><subject>Feces - chemistry</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Genome, Bacterial</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Male</subject><subject>Mechanisms</subject><subject>Middle Aged</subject><subject>Molecular biology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Saliva - 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Academic</collection><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seville, Lorna A.</au><au>Patterson, Andrea J.</au><au>Scott, Karen P.</au><au>Mullany, Peter</au><au>Quail, Michael A.</au><au>Parkhill, Julian</au><au>Ready, Derren</au><au>Wilson, Michael</au><au>Spratt, David</au><au>Roberts, Adam P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA</atitle><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle><addtitle>Microb Drug Resist</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>15</volume><issue>3</issue><spage>159</spage><epage>166</epage><pages>159-166</pages><issn>1076-6294</issn><eissn>1931-8448</eissn><abstract>We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that
tet
(M) and
tet
(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally,
tet
(Q),
tet
(O), and
tet
(O/32/O) have been shown to be common. We have also shown that
erm
(B),
erm
(V), and
erm
(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn
916
integrase in the oral metagenomes and the Tn
4451
and Tn
1549
integrase genes within the fecal metagenomes.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>19728772</pmid><doi>10.1089/mdr.2009.0916</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1076-6294 |
| ispartof | Microbial drug resistance (Larchmont, N.Y.), 2009-09, Vol.15 (3), p.159-166 |
| issn | 1076-6294 1931-8448 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_734037795 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Aged Anti-Bacterial Agents - pharmacology Antibiotics Bacteria Bacteria - drug effects Bacteria - genetics Deoxyribonucleic acid DNA DNA Transposable Elements DNA, Bacterial - analysis DNA, Bacterial - genetics Drug resistance Drug resistance in microorganisms Drug Resistance, Bacterial - genetics Erythromycin - pharmacology Feces - chemistry Feces - microbiology Female Genetic aspects Genome, Bacterial Genomics Health aspects Humans Male Mechanisms Middle Aged Molecular biology Oligonucleotide Array Sequence Analysis Saliva - chemistry Saliva - microbiology Tetracycline Resistance - genetics Young Adult |
| title | Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA |
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