Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice
Tyrosine site-specific recombinases (SSRs) including Cre and FLP are essential tools for DNA and genome engineering. Cre has long been recognized as the best SSR for genome engineering, particularly in mice. Obtaining another SSR that is as good as Cre will be a valuable addition to the genomic tool...
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| Veröffentlicht in: | Disease models & mechanisms 2009-09, Vol.2 (9-10), p.508-515 |
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| container_title | Disease models & mechanisms |
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| creator | Anastassiadis, Konstantinos Fu, Jun Patsch, Christoph Hu, Shengbiao Weidlich, Stefanie Duerschke, Kristin Buchholz, Frank Edenhofer, Frank Stewart, A Francis |
| description | Tyrosine site-specific recombinases (SSRs) including Cre and FLP are essential tools for DNA and genome engineering. Cre has long been recognized as the best SSR for genome engineering, particularly in mice. Obtaining another SSR that is as good as Cre will be a valuable addition to the genomic toolbox. To this end, we have developed and validated reagents for the Dre-rox system. These include an Escherichia coli-inducible expression vector based on the temperature-sensitive pSC101 plasmid, a mammalian expression vector based on the CAGGs promoter, a rox-lacZ reporter embryonic stem (ES) cell line based on targeting at the Rosa26 locus, the accompanying Rosa26-rox reporter mouse line, and a CAGGs-Dre deleter mouse line. We also show that a Dre-progesterone receptor shows good ligand-responsive induction properties. Furthermore, we show that there is no crossover recombination between Cre-rox or Dre-loxP. Hence, we add another set of efficient tools to the genomic toolbox, which will enable the development of more sophisticated mouse models for the analysis of gene function and disease. |
| doi_str_mv | 10.1242/dmm.003087 |
| format | Article |
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Cre has long been recognized as the best SSR for genome engineering, particularly in mice. Obtaining another SSR that is as good as Cre will be a valuable addition to the genomic toolbox. To this end, we have developed and validated reagents for the Dre-rox system. These include an Escherichia coli-inducible expression vector based on the temperature-sensitive pSC101 plasmid, a mammalian expression vector based on the CAGGs promoter, a rox-lacZ reporter embryonic stem (ES) cell line based on targeting at the Rosa26 locus, the accompanying Rosa26-rox reporter mouse line, and a CAGGs-Dre deleter mouse line. We also show that a Dre-progesterone receptor shows good ligand-responsive induction properties. Furthermore, we show that there is no crossover recombination between Cre-rox or Dre-loxP. Hence, we add another set of efficient tools to the genomic toolbox, which will enable the development of more sophisticated mouse models for the analysis of gene function and disease.</description><identifier>ISSN: 1754-8403</identifier><identifier>EISSN: 1754-8411</identifier><identifier>DOI: 10.1242/dmm.003087</identifier><identifier>PMID: 19692579</identifier><language>eng</language><publisher>England: The Company of Biologists Ltd</publisher><subject>Animals ; Artificial chromosomes ; Base Sequence ; Cell Line ; Cloning ; Cytomegalovirus ; DNA Nucleotidyltransferases - metabolism ; E coli ; Efficiency ; Embryonic Stem Cells - drug effects ; Embryonic Stem Cells - metabolism ; Engineering ; Escherichia coli - enzymology ; Escherichia coli Proteins - metabolism ; Gene expression ; Gene Targeting ; Genes, Reporter ; Genome editing ; Genomes ; Integrases - metabolism ; Ligands ; Mice ; Mifepristone - pharmacology ; Molecular Sequence Data ; Plasmids ; Plasmids - genetics ; Progesterone - metabolism ; Prokaryotic Cells - drug effects ; Prokaryotic Cells - metabolism ; Proteins - metabolism ; Recombinant Fusion Proteins - metabolism ; Recombinases - metabolism ; Recombination, Genetic - drug effects ; Recombination, Genetic - genetics ; Reproducibility of Results ; RNA, Untranslated</subject><ispartof>Disease models & mechanisms, 2009-09, Vol.2 (9-10), p.508-515</ispartof><rights>2009. 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pharmacology</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Plasmids - genetics</subject><subject>Progesterone - metabolism</subject><subject>Prokaryotic Cells - drug effects</subject><subject>Prokaryotic Cells - metabolism</subject><subject>Proteins - metabolism</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Recombinases - metabolism</subject><subject>Recombination, Genetic - drug effects</subject><subject>Recombination, Genetic - genetics</subject><subject>Reproducibility of Results</subject><subject>RNA, Untranslated</subject><issn>1754-8403</issn><issn>1754-8411</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkUtLAzEUhYMotlY3_gAJuBCkU_OazGQptT6g4EbXIZNJbOpkpiYzi_57U1pUXN3D5buHyzkAXGI0w4SRu9r7GUIUlcURGOMiZ1nJMD7-0YiOwFmMa4Q4Kak4BSMsuCB5IcagewgGBqM7X7lWRTOFjfs0cB6SchEquHIfq2YLjbVOO9P2MLreZHFjtEubv6fQtXAxg7pr3BR65b1qnGqhNk2TjNoaeqfNOTixqonm4jAn4P1x8TZ_zpavTy_z-2WmGWZ9JlAlCMI5yiuBiLC65sJUrKTWCmIVYtwqgQVTqNKs0DkpqKBclbXNc8FRRSfgZu-7Cd3XYGIvvYu7V1RruiHKgqZYOGYikdf_yHU3hDY9JwkXvCwSkyfqdk_p0MUYjJWb4LwKW4mR3LUgUwty30KCrw6WQ-VN_YseYqffefOAqg</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Anastassiadis, Konstantinos</creator><creator>Fu, Jun</creator><creator>Patsch, Christoph</creator><creator>Hu, Shengbiao</creator><creator>Weidlich, Stefanie</creator><creator>Duerschke, Kristin</creator><creator>Buchholz, Frank</creator><creator>Edenhofer, Frank</creator><creator>Stewart, A Francis</creator><general>The Company of Biologists Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20090901</creationdate><title>Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice</title><author>Anastassiadis, Konstantinos ; Fu, Jun ; Patsch, Christoph ; Hu, Shengbiao ; Weidlich, Stefanie ; Duerschke, Kristin ; Buchholz, Frank ; Edenhofer, Frank ; Stewart, A Francis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-90b9201505b9029fcd69eb483ff92fa046fa9194a0bc47c5273936a8df55960b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Artificial chromosomes</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>Cloning</topic><topic>Cytomegalovirus</topic><topic>DNA Nucleotidyltransferases - 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Academic</collection><jtitle>Disease models & mechanisms</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anastassiadis, Konstantinos</au><au>Fu, Jun</au><au>Patsch, Christoph</au><au>Hu, Shengbiao</au><au>Weidlich, Stefanie</au><au>Duerschke, Kristin</au><au>Buchholz, Frank</au><au>Edenhofer, Frank</au><au>Stewart, A Francis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice</atitle><jtitle>Disease models & mechanisms</jtitle><addtitle>Dis Model Mech</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>2</volume><issue>9-10</issue><spage>508</spage><epage>515</epage><pages>508-515</pages><issn>1754-8403</issn><eissn>1754-8411</eissn><abstract>Tyrosine site-specific recombinases (SSRs) including Cre and FLP are essential tools for DNA and genome engineering. Cre has long been recognized as the best SSR for genome engineering, particularly in mice. Obtaining another SSR that is as good as Cre will be a valuable addition to the genomic toolbox. To this end, we have developed and validated reagents for the Dre-rox system. These include an Escherichia coli-inducible expression vector based on the temperature-sensitive pSC101 plasmid, a mammalian expression vector based on the CAGGs promoter, a rox-lacZ reporter embryonic stem (ES) cell line based on targeting at the Rosa26 locus, the accompanying Rosa26-rox reporter mouse line, and a CAGGs-Dre deleter mouse line. We also show that a Dre-progesterone receptor shows good ligand-responsive induction properties. Furthermore, we show that there is no crossover recombination between Cre-rox or Dre-loxP. Hence, we add another set of efficient tools to the genomic toolbox, which will enable the development of more sophisticated mouse models for the analysis of gene function and disease.</abstract><cop>England</cop><pub>The Company of Biologists Ltd</pub><pmid>19692579</pmid><doi>10.1242/dmm.003087</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Artificial chromosomes Base Sequence Cell Line Cloning Cytomegalovirus DNA Nucleotidyltransferases - metabolism E coli Efficiency Embryonic Stem Cells - drug effects Embryonic Stem Cells - metabolism Engineering Escherichia coli - enzymology Escherichia coli Proteins - metabolism Gene expression Gene Targeting Genes, Reporter Genome editing Genomes Integrases - metabolism Ligands Mice Mifepristone - pharmacology Molecular Sequence Data Plasmids Plasmids - genetics Progesterone - metabolism Prokaryotic Cells - drug effects Prokaryotic Cells - metabolism Proteins - metabolism Recombinant Fusion Proteins - metabolism Recombinases - metabolism Recombination, Genetic - drug effects Recombination, Genetic - genetics Reproducibility of Results RNA, Untranslated |
| title | Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice |
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