Induction of specific and flavivirus—Cross-reactive CTLs by immunization with a single dengue virus-derived CTL epitope peptide
Specificities of cytotoxic T lymphocyte (CTL) effector cells induced in BALB/c mouse by immunization with the single modified CTL epitope peptide derived from NS3 of dengue virus types 1 and 3, or that of dengue virus types 2 and 4 were examined. The effector cells included CTLs specific for the epi...
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| Veröffentlicht in: | Virus research 2009-09, Vol.144 (1), p.188-194 |
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| creator | Masaki, Hideyuki Fujii, Yoshiki Wakasa-Morimoto, Chiaki Toyosaki-Maeda, Tomoko Irimajiri, Kiyohiro Tomura, Takanori T. Kurane, Ichiro |
| description | Specificities of cytotoxic T lymphocyte (CTL) effector cells induced in BALB/c mouse by immunization with the single modified CTL epitope peptide derived from NS3 of dengue virus types 1 and 3, or that of dengue virus types 2 and 4 were examined. The effector cells included CTLs specific for the epitope peptide used for immunization and those cross-reactive to epitope peptides of other flaviviruses. A CTL clone, 2F7, was established from the effector cells. The clone 2F7 was specific for the epitope peptide used for immunization. Recognition by the effector cells was remarkably impaired by amino acid substitutions at positions 3, 5, and 6 of the epitope peptides. These results indicate that immunization with a single CTL epitope peptide of dengue viruses induces serotype-specific CTLs as well as CTLs cross-reactive to the other flaviviruses, and that the a.a. residues at positions 3, 5, and 6 are critical for cross-reaction. |
| doi_str_mv | 10.1016/j.virusres.2009.04.024 |
| format | Article |
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The effector cells included CTLs specific for the epitope peptide used for immunization and those cross-reactive to epitope peptides of other flaviviruses. A CTL clone, 2F7, was established from the effector cells. The clone 2F7 was specific for the epitope peptide used for immunization. Recognition by the effector cells was remarkably impaired by amino acid substitutions at positions 3, 5, and 6 of the epitope peptides. These results indicate that immunization with a single CTL epitope peptide of dengue viruses induces serotype-specific CTLs as well as CTLs cross-reactive to the other flaviviruses, and that the a.a. residues at positions 3, 5, and 6 are critical for cross-reaction.</description><subject>Amino Acid Substitution - genetics</subject><subject>Amino Acid Substitution - immunology</subject><subject>Animals</subject><subject>Cross Reactions</subject><subject>Cross-reactive</subject><subject>CTL</subject><subject>Dengue virus</subject><subject>Dengue Virus - immunology</subject><subject>Epitope</subject><subject>Epitopes, T-Lymphocyte - genetics</subject><subject>Epitopes, T-Lymphocyte - immunology</subject><subject>Female</subject><subject>Flavivirus</subject><subject>Immunization</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Peptide</subject><subject>RNA Helicases - genetics</subject><subject>RNA Helicases - immunology</subject><subject>Serine Endopeptidases - genetics</subject><subject>Serine Endopeptidases - immunology</subject><subject>Serotype-specific</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Viral Nonstructural Proteins - genetics</subject><subject>Viral Nonstructural Proteins - immunology</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcluFDEURS1ERJokvxB5BasqPKVs70AthkgtsUnWlst-Dm7VhF3VKFnBP_CFfAnuAbGD1duce590D0LXlNSU0ObNtt7FtOQEuWaE6JqImjDxDK2okqySQrPnaFVAVVFJ2Dl6mfOWENJw2bxA51QLJrlgK_TjdvCLm-M44DHgPIGLITpsB49DZ3fx8OXX95_rNOZcJbCF3QFe320ybh9x7PtliE_2UPAtzl-wxTkODx1gD8PDAvhQUHlIJeb3OQxTnMcJ8ATTHD1corNguwxXp3uB7j-8v1t_qjafP96u320qx7WaqxtLGHPBcqmDUF42N8KqoJ1t26CFdy21QoJuuVJMtZQHB1p6sBpaTYIi_AK9PvZOafy6QJ5NH7ODrrMDjEs2ZQ_Cy26qkK_-STIiNaeaF7A5gm6_ToJgphR7mx4NJWavyWzNH01mr8kQYYqmErw-fVjaHvzf2MlLAd4eASiL7CIkk12EwYGPCdxs_Bj_9-M3is2r0A</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Masaki, Hideyuki</creator><creator>Fujii, Yoshiki</creator><creator>Wakasa-Morimoto, Chiaki</creator><creator>Toyosaki-Maeda, Tomoko</creator><creator>Irimajiri, Kiyohiro</creator><creator>Tomura, Takanori T.</creator><creator>Kurane, Ichiro</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20090901</creationdate><title>Induction of specific and flavivirus—Cross-reactive CTLs by immunization with a single dengue virus-derived CTL epitope peptide</title><author>Masaki, Hideyuki ; Fujii, Yoshiki ; Wakasa-Morimoto, Chiaki ; Toyosaki-Maeda, Tomoko ; Irimajiri, Kiyohiro ; Tomura, Takanori T. ; Kurane, Ichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-5a022cfa379f48d7654a8f9cabbf94dcb1a47e9b38828b13fce97dea9eb90f803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amino Acid Substitution - genetics</topic><topic>Amino Acid Substitution - immunology</topic><topic>Animals</topic><topic>Cross Reactions</topic><topic>Cross-reactive</topic><topic>CTL</topic><topic>Dengue virus</topic><topic>Dengue Virus - immunology</topic><topic>Epitope</topic><topic>Epitopes, T-Lymphocyte - genetics</topic><topic>Epitopes, T-Lymphocyte - immunology</topic><topic>Female</topic><topic>Flavivirus</topic><topic>Immunization</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Peptide</topic><topic>RNA Helicases - genetics</topic><topic>RNA Helicases - immunology</topic><topic>Serine Endopeptidases - genetics</topic><topic>Serine Endopeptidases - immunology</topic><topic>Serotype-specific</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Viral Nonstructural Proteins - genetics</topic><topic>Viral Nonstructural Proteins - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Masaki, Hideyuki</creatorcontrib><creatorcontrib>Fujii, Yoshiki</creatorcontrib><creatorcontrib>Wakasa-Morimoto, Chiaki</creatorcontrib><creatorcontrib>Toyosaki-Maeda, Tomoko</creatorcontrib><creatorcontrib>Irimajiri, Kiyohiro</creatorcontrib><creatorcontrib>Tomura, Takanori T.</creatorcontrib><creatorcontrib>Kurane, Ichiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masaki, Hideyuki</au><au>Fujii, Yoshiki</au><au>Wakasa-Morimoto, Chiaki</au><au>Toyosaki-Maeda, Tomoko</au><au>Irimajiri, Kiyohiro</au><au>Tomura, Takanori T.</au><au>Kurane, Ichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of specific and flavivirus—Cross-reactive CTLs by immunization with a single dengue virus-derived CTL epitope peptide</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>144</volume><issue>1</issue><spage>188</spage><epage>194</epage><pages>188-194</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>Specificities of cytotoxic T lymphocyte (CTL) effector cells induced in BALB/c mouse by immunization with the single modified CTL epitope peptide derived from NS3 of dengue virus types 1 and 3, or that of dengue virus types 2 and 4 were examined. The effector cells included CTLs specific for the epitope peptide used for immunization and those cross-reactive to epitope peptides of other flaviviruses. A CTL clone, 2F7, was established from the effector cells. The clone 2F7 was specific for the epitope peptide used for immunization. Recognition by the effector cells was remarkably impaired by amino acid substitutions at positions 3, 5, and 6 of the epitope peptides. These results indicate that immunization with a single CTL epitope peptide of dengue viruses induces serotype-specific CTLs as well as CTLs cross-reactive to the other flaviviruses, and that the a.a. residues at positions 3, 5, and 6 are critical for cross-reaction.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>19427342</pmid><doi>10.1016/j.virusres.2009.04.024</doi><tpages>7</tpages></addata></record> |
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| subjects | Amino Acid Substitution - genetics Amino Acid Substitution - immunology Animals Cross Reactions Cross-reactive CTL Dengue virus Dengue Virus - immunology Epitope Epitopes, T-Lymphocyte - genetics Epitopes, T-Lymphocyte - immunology Female Flavivirus Immunization Mice Mice, Inbred BALB C Peptide RNA Helicases - genetics RNA Helicases - immunology Serine Endopeptidases - genetics Serine Endopeptidases - immunology Serotype-specific T-Lymphocytes, Cytotoxic - immunology Viral Nonstructural Proteins - genetics Viral Nonstructural Proteins - immunology |
| title | Induction of specific and flavivirus—Cross-reactive CTLs by immunization with a single dengue virus-derived CTL epitope peptide |
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