Self-Assembling Protein Arrays Using Electronic Semiconductor Microchips and in Vitro Translation
Protein arrays will greatly accelerate research and development in medical and biological sciences. We have used cell-free protein biosynthesis and a parallel immobilization strategy for producing protein biochips. We demonstrate a model two-protein microarray using luciferase and green fluorescent...
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| Veröffentlicht in: | Journal of proteome research 2003-05, Vol.2 (3), p.313-319 |
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| container_end_page | 319 |
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| container_title | Journal of proteome research |
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| creator | Oleinikov, Andrew V Gray, Matthew D Zhao, Jun Montgomery, Donald D Ghindilis, Andrey L Dill, Kilian |
| description | Protein arrays will greatly accelerate research and development in medical and biological sciences. We have used cell-free protein biosynthesis and a parallel immobilization strategy for producing protein biochips. We demonstrate a model two-protein microarray using luciferase and green fluorescent protein, both expressed in a cell-free system and specifically immobilized on CombiMatrix semiconductor oligonucleotide microarrays. This demonstration provides evidence for the appropriate folding, activity, robust presentation, and efficient flexible detection of proteins on the microscale. Keywords: semiconductor microchip • protein microarray • in vitro translation • self-assembly • fluorescence • electrochemical detection. |
| doi_str_mv | 10.1021/pr0300011 |
| format | Article |
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Keywords: semiconductor microchip • protein microarray • in vitro translation • self-assembly • fluorescence • electrochemical detection.</description><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Humans</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Array Analysis - methods</subject><subject>Protein Biosynthesis</subject><subject>Semiconductors</subject><subject>Staining and Labeling</subject><issn>1535-3893</issn><issn>1535-3907</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE9PwzAMxSMEYmNw4AugXEDiUHCadmmP0zT-SEMgbeNapakHmdqkJO1h355MG3DhYlvW7z3Zj5BLBncMYnbfOuAAwNgRGbKUpxHPQRz_zFnOB-TM-00gUgH8lAxYnLEkFjAkcoH1Opp4j01Za_NB35ztUBs6cU5uPV353XJWo-qcNVrRBTZaWVP1qrOOvmjlrPrUrafSVDTo3nUA6dJJ42vZaWvOycla1h4vDn1EVg-z5fQpmr8-Pk8n80hyIboozWWCoEqm4kryHBXEKeYCJVRYMlBYZqHwBBKRinzMIU9FIPMs6GJEyUfkZu_bOvvVo--KRnuFdS0N2t4XImjjcTIO4O0eDKd773BdtE430m0LBsUuz-I3z8BeHUz7ssHqjzwEGIDrPSCVLza2dyb8-I_RN4bIfK4</recordid><startdate>20030501</startdate><enddate>20030501</enddate><creator>Oleinikov, Andrew V</creator><creator>Gray, Matthew D</creator><creator>Zhao, Jun</creator><creator>Montgomery, Donald D</creator><creator>Ghindilis, Andrey L</creator><creator>Dill, Kilian</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030501</creationdate><title>Self-Assembling Protein Arrays Using Electronic Semiconductor Microchips and in Vitro Translation</title><author>Oleinikov, Andrew V ; Gray, Matthew D ; Zhao, Jun ; Montgomery, Donald D ; Ghindilis, Andrey L ; Dill, Kilian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a377t-59a4e0cb1c2da39ec025e97ea0deb10ceb80ce340475796309572da989a42eea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Humans</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Polymerase Chain Reaction</topic><topic>Protein Array Analysis - methods</topic><topic>Protein Biosynthesis</topic><topic>Semiconductors</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oleinikov, Andrew V</creatorcontrib><creatorcontrib>Gray, Matthew D</creatorcontrib><creatorcontrib>Zhao, Jun</creatorcontrib><creatorcontrib>Montgomery, Donald D</creatorcontrib><creatorcontrib>Ghindilis, Andrey L</creatorcontrib><creatorcontrib>Dill, Kilian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of proteome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oleinikov, Andrew V</au><au>Gray, Matthew D</au><au>Zhao, Jun</au><au>Montgomery, Donald D</au><au>Ghindilis, Andrey L</au><au>Dill, Kilian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Self-Assembling Protein Arrays Using Electronic Semiconductor Microchips and in Vitro Translation</atitle><jtitle>Journal of proteome research</jtitle><addtitle>J. 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| ispartof | Journal of proteome research, 2003-05, Vol.2 (3), p.313-319 |
| issn | 1535-3893 1535-3907 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Fluorescent Antibody Technique, Indirect Humans Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Protein Array Analysis - methods Protein Biosynthesis Semiconductors Staining and Labeling |
| title | Self-Assembling Protein Arrays Using Electronic Semiconductor Microchips and in Vitro Translation |
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