Neuropeptide Y inhibits α-MSH release from rat hypothalamic slices through a pertussis toxin-sensitive G protein
The arcuate nucleus of the hypothalamus contains various types of peptidergic neurons. In particular, two distinct populations of neurosecretory neurons containing neuropeptide Y (NPY)- and α-melanocyte-stimulating hormone (α-MSH)-like immunoreactivity have been identified in the arcuate nucleus. Do...
Gespeichert in:
| Veröffentlicht in: | Brain research 1992-11, Vol.596 (1), p.163-168 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 168 |
|---|---|
| container_issue | 1 |
| container_start_page | 163 |
| container_title | Brain research |
| container_volume | 596 |
| creator | Blasquez, Catherine Jégou, Sylvie Bunel, Denis Tranchand Fournier, Alain Vaudry, Hubert |
| description | The arcuate nucleus of the hypothalamus contains various types of peptidergic neurons. In particular, two distinct populations of neurosecretory neurons containing neuropeptide Y (NPY)- and α-melanocyte-stimulating hormone (α-MSH)-like immunoreactivity have been identified in the arcuate nucleus. Double-labeling immunocytochemical data have recently shown that NPY-containing fibers make synaptic contacts with proopiomelanocortin (POMC) immunoreactive neurons. We have thus investigated the possible effect of NPY on the release of α-MSH from rat hypothalamic slices in vitro, using the perifusion technique. NPY significantly inhibited KCl-stimulated α-MSH release in a dose-dependent manner. The inhibitory effect of NPY was mimicked by the Y
2 agonist, NPY-(13-36), while the Y
1 agonist, [Leu
31Pro
34]NPY, was devoid of effect. Pretreatment of hypothalamic slices with pertussis toxin (PTX) blocked the inhibitory effect of NPY, suggesting that the action of NPY on POMC neurons is mediated through a PTX-sensitive G protein. These results support the notion that NPY may play a physiological role in the regulation of α-MSH release from hypothalamic neurons. |
| doi_str_mv | 10.1016/0006-8993(92)91544-O |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73402463</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>000689939291544O</els_id><sourcerecordid>16366283</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-de84e97a10cf4ed850a65259a40fcf492cfa56cbdaea53f3dd33b88f96081bb73</originalsourceid><addsrcrecordid>eNqFkctu1TAQhi0EKofCG4DkBapgEfAtTrxBQlVpkQpnASxYWY49IUa51eNU9LF4kT4TOZyjdger0cx8c9H_E_Kcszeccf2WMaaL2hj5yojXhpdKFdsHZMPrShRaKPaQbO6Qx-QJ4s81ldKwI3LEpVRVVW7I1WdY0jTDnGMA-p3GsYtNzEhvfxefvlzQBD04BNqmaaDJZdrdzFPuXO-G6Cn20QPS3KVp-dFRR2dIeUGMa236FccCYcSY4zXQczqnKUMcn5JHresRnh3iMfn24ezr6UVxuT3_ePr-svCKV7kIUCswlePMtwpCXTKnS1Eap1i7VozwrSu1b4IDV8pWhiBlU9et0azmTVPJY3Ky37vevVoAsx0ieuh7N8K0oK2kYkJp-V-Qa6m1qHeg2oM-TYgJWjunOLh0YzmzO0vsTm-709saYf9aYrfr2IvD_qUZINwP7T1Y-y8PfYfe9W1yo494hymllZRixd7tMVhFu46QLPoIo4cQE_hswxT__ccfk5SqlA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16366283</pqid></control><display><type>article</type><title>Neuropeptide Y inhibits α-MSH release from rat hypothalamic slices through a pertussis toxin-sensitive G protein</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Blasquez, Catherine ; Jégou, Sylvie ; Bunel, Denis Tranchand ; Fournier, Alain ; Vaudry, Hubert</creator><creatorcontrib>Blasquez, Catherine ; Jégou, Sylvie ; Bunel, Denis Tranchand ; Fournier, Alain ; Vaudry, Hubert</creatorcontrib><description>The arcuate nucleus of the hypothalamus contains various types of peptidergic neurons. In particular, two distinct populations of neurosecretory neurons containing neuropeptide Y (NPY)- and α-melanocyte-stimulating hormone (α-MSH)-like immunoreactivity have been identified in the arcuate nucleus. Double-labeling immunocytochemical data have recently shown that NPY-containing fibers make synaptic contacts with proopiomelanocortin (POMC) immunoreactive neurons. We have thus investigated the possible effect of NPY on the release of α-MSH from rat hypothalamic slices in vitro, using the perifusion technique. NPY significantly inhibited KCl-stimulated α-MSH release in a dose-dependent manner. The inhibitory effect of NPY was mimicked by the Y
2 agonist, NPY-(13-36), while the Y
1 agonist, [Leu
31Pro
34]NPY, was devoid of effect. Pretreatment of hypothalamic slices with pertussis toxin (PTX) blocked the inhibitory effect of NPY, suggesting that the action of NPY on POMC neurons is mediated through a PTX-sensitive G protein. These results support the notion that NPY may play a physiological role in the regulation of α-MSH release from hypothalamic neurons.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(92)91544-O</identifier><identifier>PMID: 1334775</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>alpha-MSH - antagonists & inhibitors ; alpha-MSH - metabolism ; Animals ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; GTP-Binding Proteins - physiology ; Hormones and neuropeptides. Regulation ; Hypothalamus ; Hypothalamus - cytology ; Hypothalamus - metabolism ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; In Vitro Techniques ; Male ; Melanocyte-stimulating hormone ; Neurons - metabolism ; Neurons - physiology ; Neuropeptide release ; Neuropeptide Y ; Neuropeptide Y - pharmacology ; Neuropeptide Y - physiology ; Pertussis Toxin ; Potassium Chloride - pharmacology ; Pro-Opiomelanocortin - metabolism ; Proopiomelanocortin-containing neuron ; Rats ; Rats, Wistar ; Vertebrates: endocrinology ; Virulence Factors, Bordetella - pharmacology</subject><ispartof>Brain research, 1992-11, Vol.596 (1), p.163-168</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-de84e97a10cf4ed850a65259a40fcf492cfa56cbdaea53f3dd33b88f96081bb73</citedby><cites>FETCH-LOGICAL-c417t-de84e97a10cf4ed850a65259a40fcf492cfa56cbdaea53f3dd33b88f96081bb73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/000689939291544O$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4464332$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1334775$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blasquez, Catherine</creatorcontrib><creatorcontrib>Jégou, Sylvie</creatorcontrib><creatorcontrib>Bunel, Denis Tranchand</creatorcontrib><creatorcontrib>Fournier, Alain</creatorcontrib><creatorcontrib>Vaudry, Hubert</creatorcontrib><title>Neuropeptide Y inhibits α-MSH release from rat hypothalamic slices through a pertussis toxin-sensitive G protein</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The arcuate nucleus of the hypothalamus contains various types of peptidergic neurons. In particular, two distinct populations of neurosecretory neurons containing neuropeptide Y (NPY)- and α-melanocyte-stimulating hormone (α-MSH)-like immunoreactivity have been identified in the arcuate nucleus. Double-labeling immunocytochemical data have recently shown that NPY-containing fibers make synaptic contacts with proopiomelanocortin (POMC) immunoreactive neurons. We have thus investigated the possible effect of NPY on the release of α-MSH from rat hypothalamic slices in vitro, using the perifusion technique. NPY significantly inhibited KCl-stimulated α-MSH release in a dose-dependent manner. The inhibitory effect of NPY was mimicked by the Y
2 agonist, NPY-(13-36), while the Y
1 agonist, [Leu
31Pro
34]NPY, was devoid of effect. Pretreatment of hypothalamic slices with pertussis toxin (PTX) blocked the inhibitory effect of NPY, suggesting that the action of NPY on POMC neurons is mediated through a PTX-sensitive G protein. These results support the notion that NPY may play a physiological role in the regulation of α-MSH release from hypothalamic neurons.</description><subject>alpha-MSH - antagonists & inhibitors</subject><subject>alpha-MSH - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GTP-Binding Proteins - physiology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus</subject><subject>Hypothalamus - cytology</subject><subject>Hypothalamus - metabolism</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Melanocyte-stimulating hormone</subject><subject>Neurons - metabolism</subject><subject>Neurons - physiology</subject><subject>Neuropeptide release</subject><subject>Neuropeptide Y</subject><subject>Neuropeptide Y - pharmacology</subject><subject>Neuropeptide Y - physiology</subject><subject>Pertussis Toxin</subject><subject>Potassium Chloride - pharmacology</subject><subject>Pro-Opiomelanocortin - metabolism</subject><subject>Proopiomelanocortin-containing neuron</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vertebrates: endocrinology</subject><subject>Virulence Factors, Bordetella - pharmacology</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctu1TAQhi0EKofCG4DkBapgEfAtTrxBQlVpkQpnASxYWY49IUa51eNU9LF4kT4TOZyjdger0cx8c9H_E_Kcszeccf2WMaaL2hj5yojXhpdKFdsHZMPrShRaKPaQbO6Qx-QJ4s81ldKwI3LEpVRVVW7I1WdY0jTDnGMA-p3GsYtNzEhvfxefvlzQBD04BNqmaaDJZdrdzFPuXO-G6Cn20QPS3KVp-dFRR2dIeUGMa236FccCYcSY4zXQczqnKUMcn5JHresRnh3iMfn24ezr6UVxuT3_ePr-svCKV7kIUCswlePMtwpCXTKnS1Eap1i7VozwrSu1b4IDV8pWhiBlU9et0azmTVPJY3Ky37vevVoAsx0ieuh7N8K0oK2kYkJp-V-Qa6m1qHeg2oM-TYgJWjunOLh0YzmzO0vsTm-709saYf9aYrfr2IvD_qUZINwP7T1Y-y8PfYfe9W1yo494hymllZRixd7tMVhFu46QLPoIo4cQE_hswxT__ccfk5SqlA</recordid><startdate>19921120</startdate><enddate>19921120</enddate><creator>Blasquez, Catherine</creator><creator>Jégou, Sylvie</creator><creator>Bunel, Denis Tranchand</creator><creator>Fournier, Alain</creator><creator>Vaudry, Hubert</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19921120</creationdate><title>Neuropeptide Y inhibits α-MSH release from rat hypothalamic slices through a pertussis toxin-sensitive G protein</title><author>Blasquez, Catherine ; Jégou, Sylvie ; Bunel, Denis Tranchand ; Fournier, Alain ; Vaudry, Hubert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-de84e97a10cf4ed850a65259a40fcf492cfa56cbdaea53f3dd33b88f96081bb73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>alpha-MSH - antagonists & inhibitors</topic><topic>alpha-MSH - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GTP-Binding Proteins - physiology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus</topic><topic>Hypothalamus - cytology</topic><topic>Hypothalamus - metabolism</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Melanocyte-stimulating hormone</topic><topic>Neurons - metabolism</topic><topic>Neurons - physiology</topic><topic>Neuropeptide release</topic><topic>Neuropeptide Y</topic><topic>Neuropeptide Y - pharmacology</topic><topic>Neuropeptide Y - physiology</topic><topic>Pertussis Toxin</topic><topic>Potassium Chloride - pharmacology</topic><topic>Pro-Opiomelanocortin - metabolism</topic><topic>Proopiomelanocortin-containing neuron</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vertebrates: endocrinology</topic><topic>Virulence Factors, Bordetella - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blasquez, Catherine</creatorcontrib><creatorcontrib>Jégou, Sylvie</creatorcontrib><creatorcontrib>Bunel, Denis Tranchand</creatorcontrib><creatorcontrib>Fournier, Alain</creatorcontrib><creatorcontrib>Vaudry, Hubert</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blasquez, Catherine</au><au>Jégou, Sylvie</au><au>Bunel, Denis Tranchand</au><au>Fournier, Alain</au><au>Vaudry, Hubert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuropeptide Y inhibits α-MSH release from rat hypothalamic slices through a pertussis toxin-sensitive G protein</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1992-11-20</date><risdate>1992</risdate><volume>596</volume><issue>1</issue><spage>163</spage><epage>168</epage><pages>163-168</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The arcuate nucleus of the hypothalamus contains various types of peptidergic neurons. In particular, two distinct populations of neurosecretory neurons containing neuropeptide Y (NPY)- and α-melanocyte-stimulating hormone (α-MSH)-like immunoreactivity have been identified in the arcuate nucleus. Double-labeling immunocytochemical data have recently shown that NPY-containing fibers make synaptic contacts with proopiomelanocortin (POMC) immunoreactive neurons. We have thus investigated the possible effect of NPY on the release of α-MSH from rat hypothalamic slices in vitro, using the perifusion technique. NPY significantly inhibited KCl-stimulated α-MSH release in a dose-dependent manner. The inhibitory effect of NPY was mimicked by the Y
2 agonist, NPY-(13-36), while the Y
1 agonist, [Leu
31Pro
34]NPY, was devoid of effect. Pretreatment of hypothalamic slices with pertussis toxin (PTX) blocked the inhibitory effect of NPY, suggesting that the action of NPY on POMC neurons is mediated through a PTX-sensitive G protein. These results support the notion that NPY may play a physiological role in the regulation of α-MSH release from hypothalamic neurons.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>1334775</pmid><doi>10.1016/0006-8993(92)91544-O</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-8993 |
| ispartof | Brain research, 1992-11, Vol.596 (1), p.163-168 |
| issn | 0006-8993 1872-6240 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73402463 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | alpha-MSH - antagonists & inhibitors alpha-MSH - metabolism Animals Biological and medical sciences Fundamental and applied biological sciences. Psychology GTP-Binding Proteins - physiology Hormones and neuropeptides. Regulation Hypothalamus Hypothalamus - cytology Hypothalamus - metabolism Hypothalamus. Hypophysis. Epiphysis. Urophysis In Vitro Techniques Male Melanocyte-stimulating hormone Neurons - metabolism Neurons - physiology Neuropeptide release Neuropeptide Y Neuropeptide Y - pharmacology Neuropeptide Y - physiology Pertussis Toxin Potassium Chloride - pharmacology Pro-Opiomelanocortin - metabolism Proopiomelanocortin-containing neuron Rats Rats, Wistar Vertebrates: endocrinology Virulence Factors, Bordetella - pharmacology |
| title | Neuropeptide Y inhibits α-MSH release from rat hypothalamic slices through a pertussis toxin-sensitive G protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T21%3A43%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neuropeptide%20Y%20inhibits%20%CE%B1-MSH%20release%20from%20rat%20hypothalamic%20slices%20through%20a%20pertussis%20toxin-sensitive%20G%20protein&rft.jtitle=Brain%20research&rft.au=Blasquez,%20Catherine&rft.date=1992-11-20&rft.volume=596&rft.issue=1&rft.spage=163&rft.epage=168&rft.pages=163-168&rft.issn=0006-8993&rft.eissn=1872-6240&rft.coden=BRREAP&rft_id=info:doi/10.1016/0006-8993(92)91544-O&rft_dat=%3Cproquest_cross%3E16366283%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16366283&rft_id=info:pmid/1334775&rft_els_id=000689939291544O&rfr_iscdi=true |