Optimization of a novel nylon mesh container for human embryo ultrarapid vitrification
Objective To evaluate the efficacy of a nylon mesh container in vitrification of human embryos and to determine the optimal osmotic pressure of the initial thawing solution. Design Retrospective analysis. Setting National Center for Child Health and Development, Tokyo, Japan. Patient(s) Infertile pa...
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| Veröffentlicht in: | Fertility and sterility 2010-05, Vol.93 (7), p.2405-2410 |
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| creator | Nakashima, Akira, M.D Ino, Nao, M.D Kusumi, Maki, M.D., Ph.D Ohgi, Shirei, M.D Ito, Megumu, M.D Horikawa, Takashi, M.D Nakagawa, Koji, M.D., Ph.D Saito, Takakazu, M.D., Ph.D Kamura, Toshiharu, M.D., Ph.D Saito, Hidekazu, M.D., Ph.D |
| description | Objective To evaluate the efficacy of a nylon mesh container in vitrification of human embryos and to determine the optimal osmotic pressure of the initial thawing solution. Design Retrospective analysis. Setting National Center for Child Health and Development, Tokyo, Japan. Patient(s) Infertile patients undergoing either in vitro fertilization or intracytoplasmic sperm injection in our hospital. Intervention(s) Embryos, at the cleavage stage, were cryopreserved using the vitrification method in either a plastic straw or a nylon mesh container. The embryos were thawed using an initial osmotic pressure of either 0.5 M or 1.0 M sucrose with subsequent step-wise dilution. After thawing, the embryos were transferred to the uterus. Main Outcome Measure(s) Survival rate of blastomeres, embryo survival rate, implantation, and pregnancy rates, cancellation rate because of embryo damage. Result(s) Use of nylon mesh and the 1.0 M sucrose thawing solution significantly improved blastomere survival rate (98.0 ± 1.0%, mean ± SEM), pregnancy rate (41.0%) and implantation rate (32.3%). Conclusion(s) Vitrification using a nylon mesh container and subsequent thawing in a 1.0 M sucrose solution is an easy and inexpensive method that improves the reliability of embryo cryopreservation of embryos without adverse effects on clinical outcomes. |
| doi_str_mv | 10.1016/j.fertnstert.2009.01.063 |
| format | Article |
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Design Retrospective analysis. Setting National Center for Child Health and Development, Tokyo, Japan. Patient(s) Infertile patients undergoing either in vitro fertilization or intracytoplasmic sperm injection in our hospital. Intervention(s) Embryos, at the cleavage stage, were cryopreserved using the vitrification method in either a plastic straw or a nylon mesh container. The embryos were thawed using an initial osmotic pressure of either 0.5 M or 1.0 M sucrose with subsequent step-wise dilution. After thawing, the embryos were transferred to the uterus. Main Outcome Measure(s) Survival rate of blastomeres, embryo survival rate, implantation, and pregnancy rates, cancellation rate because of embryo damage. Result(s) Use of nylon mesh and the 1.0 M sucrose thawing solution significantly improved blastomere survival rate (98.0 ± 1.0%, mean ± SEM), pregnancy rate (41.0%) and implantation rate (32.3%). Conclusion(s) Vitrification using a nylon mesh container and subsequent thawing in a 1.0 M sucrose solution is an easy and inexpensive method that improves the reliability of embryo cryopreservation of embryos without adverse effects on clinical outcomes.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2009.01.063</identifier><identifier>PMID: 19230875</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Biological and medical sciences ; Calibration ; Cell Survival - drug effects ; cryopreservation ; Cryopreservation - instrumentation ; Cryopreservation - methods ; Cryopreservation - standards ; Cryoprotective Agents - pharmacology ; Embryo Implantation - drug effects ; Embryo Implantation - physiology ; Embryo, Mammalian ; Female ; Fetal Viability - drug effects ; Gynecology. Andrology. Obstetrics ; Humans ; Internal Medicine ; Male ; Medical sciences ; Models, Biological ; nylon mesh ; Nylons - pharmacology ; Obstetrics and Gynecology ; Plastics - pharmacology ; Pregnancy ; Pregnancy Rate ; Product Packaging ; Retrospective Studies ; Surgical Mesh ; thawing solution ; Time Factors ; Ultrarapid vitrification</subject><ispartof>Fertility and sterility, 2010-05, Vol.93 (7), p.2405-2410</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2010 American Society for Reproductive Medicine</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c574t-f76ab81f1582b93279fc66412495b7e5dfef7a9cec95690656970de1b46193723</citedby><cites>FETCH-LOGICAL-c574t-f76ab81f1582b93279fc66412495b7e5dfef7a9cec95690656970de1b46193723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fertnstert.2009.01.063$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22805470$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19230875$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakashima, Akira, M.D</creatorcontrib><creatorcontrib>Ino, Nao, M.D</creatorcontrib><creatorcontrib>Kusumi, Maki, M.D., Ph.D</creatorcontrib><creatorcontrib>Ohgi, Shirei, M.D</creatorcontrib><creatorcontrib>Ito, Megumu, M.D</creatorcontrib><creatorcontrib>Horikawa, Takashi, M.D</creatorcontrib><creatorcontrib>Nakagawa, Koji, M.D., Ph.D</creatorcontrib><creatorcontrib>Saito, Takakazu, M.D., Ph.D</creatorcontrib><creatorcontrib>Kamura, Toshiharu, M.D., Ph.D</creatorcontrib><creatorcontrib>Saito, Hidekazu, M.D., Ph.D</creatorcontrib><title>Optimization of a novel nylon mesh container for human embryo ultrarapid vitrification</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective To evaluate the efficacy of a nylon mesh container in vitrification of human embryos and to determine the optimal osmotic pressure of the initial thawing solution. Design Retrospective analysis. Setting National Center for Child Health and Development, Tokyo, Japan. Patient(s) Infertile patients undergoing either in vitro fertilization or intracytoplasmic sperm injection in our hospital. Intervention(s) Embryos, at the cleavage stage, were cryopreserved using the vitrification method in either a plastic straw or a nylon mesh container. The embryos were thawed using an initial osmotic pressure of either 0.5 M or 1.0 M sucrose with subsequent step-wise dilution. After thawing, the embryos were transferred to the uterus. Main Outcome Measure(s) Survival rate of blastomeres, embryo survival rate, implantation, and pregnancy rates, cancellation rate because of embryo damage. Result(s) Use of nylon mesh and the 1.0 M sucrose thawing solution significantly improved blastomere survival rate (98.0 ± 1.0%, mean ± SEM), pregnancy rate (41.0%) and implantation rate (32.3%). Conclusion(s) Vitrification using a nylon mesh container and subsequent thawing in a 1.0 M sucrose solution is an easy and inexpensive method that improves the reliability of embryo cryopreservation of embryos without adverse effects on clinical outcomes.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Cell Survival - drug effects</subject><subject>cryopreservation</subject><subject>Cryopreservation - instrumentation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - standards</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Embryo Implantation - drug effects</subject><subject>Embryo Implantation - physiology</subject><subject>Embryo, Mammalian</subject><subject>Female</subject><subject>Fetal Viability - drug effects</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Models, Biological</subject><subject>nylon mesh</subject><subject>Nylons - pharmacology</subject><subject>Obstetrics and Gynecology</subject><subject>Plastics - pharmacology</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Product Packaging</subject><subject>Retrospective Studies</subject><subject>Surgical Mesh</subject><subject>thawing solution</subject><subject>Time Factors</subject><subject>Ultrarapid vitrification</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2LFDEQhhtR3NnVvyC5iKduK-lO0rkIuqgrLOzBj2tIpytsxu5kTLoHZn-9GWdwwZOXSghPvRUeqqoIhYYCFW-3jcO0hLyU2jAA1QBtQLRPqg3lXNRc8PZptQGgvAbWs4vqMuctAAgq2fPqgirWQi_5pvpxt1v87B_M4mMg0RFDQtzjRMJhKg8z5ntiY1iMD5iIi4ncr7MJBOchHSJZpyWZZHZ-JHu_JO-8_ZP0onrmzJTx5fm8qr5_-vjt-qa-vfv85fr9bW257JbaSWGGnjrKezaolknlrBAdZZ3ig0Q-OnTSKItWcaFAlCJhRDp0gqpWsvaqenPK3aX4a8W86Nlni9NkAsY1a9l2wDoOqpD9ibQp5pzQ6V3ys0kHTUEfpeqtfpSqj1I1UF2kltZX5yHrMOP42Hi2WIDXZ8BkayaXTLA-_-UY64F3Egr34cRhUbL3mHS2HoPF0Se0ix6j_5_fvPsnxE4-FO_TTzxg3sY1haJcU52ZBv31uATHHQBVbrQX7W-MpLBF</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Nakashima, Akira, M.D</creator><creator>Ino, Nao, M.D</creator><creator>Kusumi, Maki, M.D., Ph.D</creator><creator>Ohgi, Shirei, M.D</creator><creator>Ito, Megumu, M.D</creator><creator>Horikawa, Takashi, M.D</creator><creator>Nakagawa, Koji, M.D., Ph.D</creator><creator>Saito, Takakazu, M.D., Ph.D</creator><creator>Kamura, Toshiharu, M.D., Ph.D</creator><creator>Saito, Hidekazu, M.D., Ph.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100501</creationdate><title>Optimization of a novel nylon mesh container for human embryo ultrarapid vitrification</title><author>Nakashima, Akira, M.D ; Ino, Nao, M.D ; Kusumi, Maki, M.D., Ph.D ; Ohgi, Shirei, M.D ; Ito, Megumu, M.D ; Horikawa, Takashi, M.D ; Nakagawa, Koji, M.D., Ph.D ; Saito, Takakazu, M.D., Ph.D ; Kamura, Toshiharu, M.D., Ph.D ; Saito, Hidekazu, M.D., Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c574t-f76ab81f1582b93279fc66412495b7e5dfef7a9cec95690656970de1b46193723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Cell Survival - drug effects</topic><topic>cryopreservation</topic><topic>Cryopreservation - instrumentation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - standards</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Embryo Implantation - drug effects</topic><topic>Embryo Implantation - physiology</topic><topic>Embryo, Mammalian</topic><topic>Female</topic><topic>Fetal Viability - drug effects</topic><topic>Gynecology. 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Obstetrics</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Models, Biological</topic><topic>nylon mesh</topic><topic>Nylons - pharmacology</topic><topic>Obstetrics and Gynecology</topic><topic>Plastics - pharmacology</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Product Packaging</topic><topic>Retrospective Studies</topic><topic>Surgical Mesh</topic><topic>thawing solution</topic><topic>Time Factors</topic><topic>Ultrarapid vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakashima, Akira, M.D</creatorcontrib><creatorcontrib>Ino, Nao, M.D</creatorcontrib><creatorcontrib>Kusumi, Maki, M.D., Ph.D</creatorcontrib><creatorcontrib>Ohgi, Shirei, M.D</creatorcontrib><creatorcontrib>Ito, Megumu, M.D</creatorcontrib><creatorcontrib>Horikawa, Takashi, M.D</creatorcontrib><creatorcontrib>Nakagawa, Koji, M.D., Ph.D</creatorcontrib><creatorcontrib>Saito, Takakazu, M.D., Ph.D</creatorcontrib><creatorcontrib>Kamura, Toshiharu, M.D., Ph.D</creatorcontrib><creatorcontrib>Saito, Hidekazu, M.D., Ph.D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakashima, Akira, M.D</au><au>Ino, Nao, M.D</au><au>Kusumi, Maki, M.D., Ph.D</au><au>Ohgi, Shirei, M.D</au><au>Ito, Megumu, M.D</au><au>Horikawa, Takashi, M.D</au><au>Nakagawa, Koji, M.D., Ph.D</au><au>Saito, Takakazu, M.D., Ph.D</au><au>Kamura, Toshiharu, M.D., Ph.D</au><au>Saito, Hidekazu, M.D., Ph.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of a novel nylon mesh container for human embryo ultrarapid vitrification</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>93</volume><issue>7</issue><spage>2405</spage><epage>2410</epage><pages>2405-2410</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective To evaluate the efficacy of a nylon mesh container in vitrification of human embryos and to determine the optimal osmotic pressure of the initial thawing solution. Design Retrospective analysis. Setting National Center for Child Health and Development, Tokyo, Japan. Patient(s) Infertile patients undergoing either in vitro fertilization or intracytoplasmic sperm injection in our hospital. Intervention(s) Embryos, at the cleavage stage, were cryopreserved using the vitrification method in either a plastic straw or a nylon mesh container. The embryos were thawed using an initial osmotic pressure of either 0.5 M or 1.0 M sucrose with subsequent step-wise dilution. After thawing, the embryos were transferred to the uterus. Main Outcome Measure(s) Survival rate of blastomeres, embryo survival rate, implantation, and pregnancy rates, cancellation rate because of embryo damage. Result(s) Use of nylon mesh and the 1.0 M sucrose thawing solution significantly improved blastomere survival rate (98.0 ± 1.0%, mean ± SEM), pregnancy rate (41.0%) and implantation rate (32.3%). Conclusion(s) Vitrification using a nylon mesh container and subsequent thawing in a 1.0 M sucrose solution is an easy and inexpensive method that improves the reliability of embryo cryopreservation of embryos without adverse effects on clinical outcomes.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>19230875</pmid><doi>10.1016/j.fertnstert.2009.01.063</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adult Biological and medical sciences Calibration Cell Survival - drug effects cryopreservation Cryopreservation - instrumentation Cryopreservation - methods Cryopreservation - standards Cryoprotective Agents - pharmacology Embryo Implantation - drug effects Embryo Implantation - physiology Embryo, Mammalian Female Fetal Viability - drug effects Gynecology. Andrology. Obstetrics Humans Internal Medicine Male Medical sciences Models, Biological nylon mesh Nylons - pharmacology Obstetrics and Gynecology Plastics - pharmacology Pregnancy Pregnancy Rate Product Packaging Retrospective Studies Surgical Mesh thawing solution Time Factors Ultrarapid vitrification |
| title | Optimization of a novel nylon mesh container for human embryo ultrarapid vitrification |
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