Chemiluminescence-HPLC assay of phosphatidylcholine hydroperoxide generated by ischemia-reperfusion in the liver of rats

To determine cellular damage due to “oxidative stress”, we developed a sensitive and specific quantitative assay for phosphatidylcholine hydroperoxide (PCOOH) by coupling HPLC with detection of chemiluminescence (CL). The qualitative and quantitative detection limits of PCOOH by this assay were 0.5...

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Veröffentlicht in:	Biochemical pharmacology 1992-12, Vol.44 (12), p.2412-2414
Hauptverfasser:	Takayama, Fusako, Egashira, Toru, Kudo, Yoshikuni, Yamanaka, Yasumitsu
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of complex biological substances Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Blood and biological fluids Chromatography, High Pressure Liquid - methods Free Radicals Fundamental and applied biological sciences. Psychology Infarction - metabolism Liver - blood supply Liver - metabolism Luminescent Measurements Male Phosphatidylcholines - analysis Rats Rats, Wistar Reperfusion - methods Time Factors
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