Phylogenetic and metabolic diversity of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-transforming bacteria in strictly anaerobic mixed cultures enriched on RDX as nitrogen source
Five obligate anaerobes that were most closely related to Clostridium bifermentans, Clostridium celerecrescens, Clostridium saccharolyticum, Clostridium butyricum and Desulfovibrio desulfuricans by their 16S rRNA genes sequences were isolated from enrichment cultures using hexahydro-1,3,5-trinitro-1...
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| Veröffentlicht in: | FEMS microbiology ecology 2003-11, Vol.46 (2), p.189-196 |
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| creator | Zhao, Jian-Shen Spain, Jim Hawari, Jalal |
| description | Five obligate anaerobes that were most closely related to
Clostridium bifermentans,
Clostridium celerecrescens,
Clostridium saccharolyticum,
Clostridium butyricum and
Desulfovibrio desulfuricans by their 16S rRNA genes sequences were isolated from enrichment cultures using hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) as a nitrogen source. The above isolates transformed RDX at rates of 24.0, 5.4, 6.2, 2.5, 5.5 μmol h
−1 g (dry weight) of cells
−1, respectively, to nitrite, formaldehyde, methanol, and nitrous oxide. The present results indicate that clostridia are major strains responsible for RDX removal, and all isolates seemed to mainly transform RDX via its initial reduction to MNX and subsequent denitration. Since clostridia are commonly present in soil, we suggest that they may contribute to the removal of RDX in the subsurface (anoxic) soil. |
| doi_str_mv | 10.1016/S0168-6496(03)00216-2 |
| format | Article |
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Clostridium bifermentans,
Clostridium celerecrescens,
Clostridium saccharolyticum,
Clostridium butyricum and
Desulfovibrio desulfuricans by their 16S rRNA genes sequences were isolated from enrichment cultures using hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) as a nitrogen source. The above isolates transformed RDX at rates of 24.0, 5.4, 6.2, 2.5, 5.5 μmol h
−1 g (dry weight) of cells
−1, respectively, to nitrite, formaldehyde, methanol, and nitrous oxide. The present results indicate that clostridia are major strains responsible for RDX removal, and all isolates seemed to mainly transform RDX via its initial reduction to MNX and subsequent denitration. Since clostridia are commonly present in soil, we suggest that they may contribute to the removal of RDX in the subsurface (anoxic) soil.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1016/S0168-6496(03)00216-2</identifier><identifier>PMID: 19719572</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Agronomy. Soil science and plant productions ; Biodegradation of pollutants ; Biological and medical sciences ; Biotechnology ; Clostridium ; Clostridium bifermentans ; Denitration ; Desulfovibrio ; Desulfovibrio desulfuricans ; Economic plant physiology ; Enrichment culture ; Environment and pollution ; formaldehyde ; Fundamental and applied biological sciences. Psychology ; hexahydro-1,3,5-trinitro-1,3,5-triazine ; Industrial applications and implications. Economical aspects ; methanol ; Nitramine explosive ; Nitroso compound ; nitrous oxide ; Phylogeny ; RDX biotransformation ; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><ispartof>FEMS microbiology ecology, 2003-11, Vol.46 (2), p.189-196</ispartof><rights>2003 Federation of European Microbiological Societies</rights><rights>2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. 2003</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6259-5ba029177dfdfa64aeb662d590deeeb4cb260a07aefd85118801a5ee0f939cb03</citedby><cites>FETCH-LOGICAL-c6259-5ba029177dfdfa64aeb662d590deeeb4cb260a07aefd85118801a5ee0f939cb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0168-6496%2803%2900216-2$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0168-6496%2803%2900216-2$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15208919$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19719572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Jian-Shen</creatorcontrib><creatorcontrib>Spain, Jim</creatorcontrib><creatorcontrib>Hawari, Jalal</creatorcontrib><title>Phylogenetic and metabolic diversity of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-transforming bacteria in strictly anaerobic mixed cultures enriched on RDX as nitrogen source</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Five obligate anaerobes that were most closely related to
Clostridium bifermentans,
Clostridium celerecrescens,
Clostridium saccharolyticum,
Clostridium butyricum and
Desulfovibrio desulfuricans by their 16S rRNA genes sequences were isolated from enrichment cultures using hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) as a nitrogen source. The above isolates transformed RDX at rates of 24.0, 5.4, 6.2, 2.5, 5.5 μmol h
−1 g (dry weight) of cells
−1, respectively, to nitrite, formaldehyde, methanol, and nitrous oxide. The present results indicate that clostridia are major strains responsible for RDX removal, and all isolates seemed to mainly transform RDX via its initial reduction to MNX and subsequent denitration. Since clostridia are commonly present in soil, we suggest that they may contribute to the removal of RDX in the subsurface (anoxic) soil.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biodegradation of pollutants</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Clostridium</subject><subject>Clostridium bifermentans</subject><subject>Denitration</subject><subject>Desulfovibrio</subject><subject>Desulfovibrio desulfuricans</subject><subject>Economic plant physiology</subject><subject>Enrichment culture</subject><subject>Environment and pollution</subject><subject>formaldehyde</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hexahydro-1,3,5-trinitro-1,3,5-triazine</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>methanol</subject><subject>Nitramine explosive</subject><subject>Nitroso compound</subject><subject>nitrous oxide</subject><subject>Phylogeny</subject><subject>RDX biotransformation</subject><subject>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqNkd1u1DAQhS0EosvCI4B8Q2mlBmwnTuIrhEpLKxWB-JG4sxx70jVK7MVOSsNr8YI4uysKEgJubI39nTn2HIQeUvKUElo-e5-WOisLUR6Q_JAQRsuM3UILyqsiK0VBb6PFT2QP3YvxMyGU5wW5i_aoqKjgFVug729XU-cvwcFgNVbO4B4G1fguVcZeQYh2mLBv8Qqu1WoywWf0KD_i2RCss8OvpfpmHeCDdy8_HaZSudj60Ft3iRulB0j32DocE6iHbkpWCoJvkk1vr8FgPXbDGCBicIlYpRPvcOqFVcQbo_RGHP0YNNxHd1rVRXiw25fo4-nJh-Oz7OLNq_PjFxeZLhkXGW8UYYJWlWlNq8pCQVOWzHBBDAA0hW5YSRSpFLSm5pTWNaGKA5BW5EI3JF-iJ9u-6-C_jBAH2duooeuUAz9GWaVhMjYPdYn2_0pSkWKhtEog34I6-BgDtHIdbK_CJCmRc65yk6ucQ5Mkl5tcJUu6RzuDsenB3Kh2QSbg8Q5QUauuTfPXNt5wnJFaUJE4seW-2g6m_3OXpyevaT1ryVbrx_Wfldlvymx-1vOtBFJOVxaCjNqC02BsAD1I4-0_fv4DozjgNg</recordid><startdate>200311</startdate><enddate>200311</enddate><creator>Zhao, Jian-Shen</creator><creator>Spain, Jim</creator><creator>Hawari, Jalal</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200311</creationdate><title>Phylogenetic and metabolic diversity of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-transforming bacteria in strictly anaerobic mixed cultures enriched on RDX as nitrogen source</title><author>Zhao, Jian-Shen ; Spain, Jim ; Hawari, Jalal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6259-5ba029177dfdfa64aeb662d590deeeb4cb260a07aefd85118801a5ee0f939cb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biodegradation of pollutants</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Clostridium</topic><topic>Clostridium bifermentans</topic><topic>Denitration</topic><topic>Desulfovibrio</topic><topic>Desulfovibrio desulfuricans</topic><topic>Economic plant physiology</topic><topic>Enrichment culture</topic><topic>Environment and pollution</topic><topic>formaldehyde</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hexahydro-1,3,5-trinitro-1,3,5-triazine</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>methanol</topic><topic>Nitramine explosive</topic><topic>Nitroso compound</topic><topic>nitrous oxide</topic><topic>Phylogeny</topic><topic>RDX biotransformation</topic><topic>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Jian-Shen</creatorcontrib><creatorcontrib>Spain, Jim</creatorcontrib><creatorcontrib>Hawari, Jalal</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Jian-Shen</au><au>Spain, Jim</au><au>Hawari, Jalal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phylogenetic and metabolic diversity of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-transforming bacteria in strictly anaerobic mixed cultures enriched on RDX as nitrogen source</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2003-11</date><risdate>2003</risdate><volume>46</volume><issue>2</issue><spage>189</spage><epage>196</epage><pages>189-196</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>Five obligate anaerobes that were most closely related to
Clostridium bifermentans,
Clostridium celerecrescens,
Clostridium saccharolyticum,
Clostridium butyricum and
Desulfovibrio desulfuricans by their 16S rRNA genes sequences were isolated from enrichment cultures using hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) as a nitrogen source. The above isolates transformed RDX at rates of 24.0, 5.4, 6.2, 2.5, 5.5 μmol h
−1 g (dry weight) of cells
−1, respectively, to nitrite, formaldehyde, methanol, and nitrous oxide. The present results indicate that clostridia are major strains responsible for RDX removal, and all isolates seemed to mainly transform RDX via its initial reduction to MNX and subsequent denitration. Since clostridia are commonly present in soil, we suggest that they may contribute to the removal of RDX in the subsurface (anoxic) soil.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>19719572</pmid><doi>10.1016/S0168-6496(03)00216-2</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | FEMS microbiology ecology, 2003-11, Vol.46 (2), p.189-196 |
| issn | 0168-6496 1574-6941 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_734022015 |
| source | Wiley Online Library Journals Frontfile Complete; Oxford Journals Open Access Collection; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Agronomy. Soil science and plant productions Biodegradation of pollutants Biological and medical sciences Biotechnology Clostridium Clostridium bifermentans Denitration Desulfovibrio Desulfovibrio desulfuricans Economic plant physiology Enrichment culture Environment and pollution formaldehyde Fundamental and applied biological sciences. Psychology hexahydro-1,3,5-trinitro-1,3,5-triazine Industrial applications and implications. Economical aspects methanol Nitramine explosive Nitroso compound nitrous oxide Phylogeny RDX biotransformation Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) |
| title | Phylogenetic and metabolic diversity of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-transforming bacteria in strictly anaerobic mixed cultures enriched on RDX as nitrogen source |
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