Molecular analysis of bacterial microbiota in the gut of the termite Reticulitermes speratus (Isoptera; Rhinotermitidae)
The molecular diversity and community structure of bacteria from the gut of the termite Reticulitermes speratus were analyzed by the sequencing of near-full-length 16S rRNA genes, amplified by polymerase chain reaction. The results of the analysis of 1344 clones indicated a predominance of spirochet...
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| Veröffentlicht in: | FEMS microbiology ecology 2003-05, Vol.44 (2), p.231-242 |
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| creator | Hongoh, Yuichi Ohkuma, Moriya Kudo, Toshiaki |
| description | The molecular diversity and community structure of bacteria from the gut of the termite
Reticulitermes speratus were analyzed by the sequencing of near-full-length 16S rRNA genes, amplified by polymerase chain reaction. The results of the analysis of 1344 clones indicated a predominance of spirochetes in the gut. Spirochetal clones accounted for approximately half of the analyzed clones. The clones related to
Bacteroides, Clostridia, and the candidate division Termite Group I each accounted for approximately 5–15% of the analyzed clones. The rest were comprised of Proteobacteria, Actinobacteria,
Mycoplasma and others. Using the criterion of 97% sequence identity, the clones were sorted into 268 phylotypes, including 100 clostridial, 61 spirochetal and 31
Bacteroides-related phylotypes. More than 90% of the phylotypes were found for the first time, and some constituted monophyletic clusters with sequences recovered from the gut of other termite species. |
| doi_str_mv | 10.1016/S0168-6496(03)00026-6 |
| format | Article |
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Reticulitermes speratus were analyzed by the sequencing of near-full-length 16S rRNA genes, amplified by polymerase chain reaction. The results of the analysis of 1344 clones indicated a predominance of spirochetes in the gut. Spirochetal clones accounted for approximately half of the analyzed clones. The clones related to
Bacteroides, Clostridia, and the candidate division Termite Group I each accounted for approximately 5–15% of the analyzed clones. The rest were comprised of Proteobacteria, Actinobacteria,
Mycoplasma and others. Using the criterion of 97% sequence identity, the clones were sorted into 268 phylotypes, including 100 clostridial, 61 spirochetal and 31
Bacteroides-related phylotypes. More than 90% of the phylotypes were found for the first time, and some constituted monophyletic clusters with sequences recovered from the gut of other termite species.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1016/S0168-6496(03)00026-6</identifier><identifier>PMID: 19719640</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Actinobacteria ; Animal, plant and microbial ecology ; bacteria ; Bacteroides ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; clones ; Clostridium ; community structure ; digestive system ; Diversity ; Fundamental and applied biological sciences. Psychology ; genes ; Genetics ; Gut bacterium ; intestinal microorganisms ; Microbial ecology ; Mission oriented research ; monophyly ; Mycoplasma ; Normal microflora of man and animals. Rumen ; phylotype ; polymerase chain reaction ; Proteobacteria ; Reticulitermes speratus ; ribosomal RNA ; sequence analysis ; species diversity ; Spirochaetales ; Symbiosis ; Termite</subject><ispartof>FEMS microbiology ecology, 2003-05, Vol.44 (2), p.231-242</ispartof><rights>2003 Federation of European Microbiological Societies</rights><rights>2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 2003</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6511-977d740371ef805abb016a67215e05f1b0aa29ee8667b673dbbf38d3b33786693</citedby><cites>FETCH-LOGICAL-c6511-977d740371ef805abb016a67215e05f1b0aa29ee8667b673dbbf38d3b33786693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0168-6496%2803%2900026-6$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0168-6496%2803%2900026-6$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14687121$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19719640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hongoh, Yuichi</creatorcontrib><creatorcontrib>Ohkuma, Moriya</creatorcontrib><creatorcontrib>Kudo, Toshiaki</creatorcontrib><title>Molecular analysis of bacterial microbiota in the gut of the termite Reticulitermes speratus (Isoptera; Rhinotermitidae)</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>The molecular diversity and community structure of bacteria from the gut of the termite
Reticulitermes speratus were analyzed by the sequencing of near-full-length 16S rRNA genes, amplified by polymerase chain reaction. The results of the analysis of 1344 clones indicated a predominance of spirochetes in the gut. Spirochetal clones accounted for approximately half of the analyzed clones. The clones related to
Bacteroides, Clostridia, and the candidate division Termite Group I each accounted for approximately 5–15% of the analyzed clones. The rest were comprised of Proteobacteria, Actinobacteria,
Mycoplasma and others. Using the criterion of 97% sequence identity, the clones were sorted into 268 phylotypes, including 100 clostridial, 61 spirochetal and 31
Bacteroides-related phylotypes. More than 90% of the phylotypes were found for the first time, and some constituted monophyletic clusters with sequences recovered from the gut of other termite species.</description><subject>Actinobacteria</subject><subject>Animal, plant and microbial ecology</subject><subject>bacteria</subject><subject>Bacteroides</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>clones</subject><subject>Clostridium</subject><subject>community structure</subject><subject>digestive system</subject><subject>Diversity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genetics</subject><subject>Gut bacterium</subject><subject>intestinal microorganisms</subject><subject>Microbial ecology</subject><subject>Mission oriented research</subject><subject>monophyly</subject><subject>Mycoplasma</subject><subject>Normal microflora of man and animals. Rumen</subject><subject>phylotype</subject><subject>polymerase chain reaction</subject><subject>Proteobacteria</subject><subject>Reticulitermes speratus</subject><subject>ribosomal RNA</subject><subject>sequence analysis</subject><subject>species diversity</subject><subject>Spirochaetales</subject><subject>Symbiosis</subject><subject>Termite</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqNks1u1TAQhS0EopfCIwDeAO0iYMeJnYgFQlULlVohtXRtjZ1Ja5TEwU6A-_Y4zRUVEgI2_hl9Z2Y8x4Q85ew1Z1y-uUxLlcmilgdMHDLGcpnJe2TDS1Vksi74fbL5heyRRzF-YYyXomAPyR6vFa9lwTbkx7nv0M4dBAoDdNvoIvUtNWAnDA462jsbvHF-AuoGOt0gvZ6nBVmOiendhPQCJ5eSuOWOkcYRA0xzpAen0Y8pCG_pxY0b_Mq7BvDwMXnQQhfxyW7fJ1cnx5-PPmZnnz6cHr0_y6wsOc9qpRpVMKE4thUrwZj0KJAq5yWysuWGAeQ1YiWlMlKJxphWVI0wQqgUq8U-ebXmHYP_OmOcdO-ixa6DAf0ctUoTybmqVCJf_pXklcqLgi9guYJpMjEGbPUYXA9hqznTizn61hy9TF4zoW_N0TLpnu0KzKbH5k61cyMBL3YARAtdG2CwLt5xhawUz3ni6pX77jrc_l91fXJ8notFy1atn8c_K7PflNnS9_NV0oLXcB1SS1eXeULTh-JVrUQi3q0EJie_OQw6WoeDxcYFtJNuvPvHbH4CzFDTmw</recordid><startdate>200305</startdate><enddate>200305</enddate><creator>Hongoh, Yuichi</creator><creator>Ohkuma, Moriya</creator><creator>Kudo, Toshiaki</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>200305</creationdate><title>Molecular analysis of bacterial microbiota in the gut of the termite Reticulitermes speratus (Isoptera; Rhinotermitidae)</title><author>Hongoh, Yuichi ; Ohkuma, Moriya ; Kudo, Toshiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6511-977d740371ef805abb016a67215e05f1b0aa29ee8667b673dbbf38d3b33786693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Actinobacteria</topic><topic>Animal, plant and microbial ecology</topic><topic>bacteria</topic><topic>Bacteroides</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>clones</topic><topic>Clostridium</topic><topic>community structure</topic><topic>digestive system</topic><topic>Diversity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genetics</topic><topic>Gut bacterium</topic><topic>intestinal microorganisms</topic><topic>Microbial ecology</topic><topic>Mission oriented research</topic><topic>monophyly</topic><topic>Mycoplasma</topic><topic>Normal microflora of man and animals. Rumen</topic><topic>phylotype</topic><topic>polymerase chain reaction</topic><topic>Proteobacteria</topic><topic>Reticulitermes speratus</topic><topic>ribosomal RNA</topic><topic>sequence analysis</topic><topic>species diversity</topic><topic>Spirochaetales</topic><topic>Symbiosis</topic><topic>Termite</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hongoh, Yuichi</creatorcontrib><creatorcontrib>Ohkuma, Moriya</creatorcontrib><creatorcontrib>Kudo, Toshiaki</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hongoh, Yuichi</au><au>Ohkuma, Moriya</au><au>Kudo, Toshiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular analysis of bacterial microbiota in the gut of the termite Reticulitermes speratus (Isoptera; Rhinotermitidae)</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2003-05</date><risdate>2003</risdate><volume>44</volume><issue>2</issue><spage>231</spage><epage>242</epage><pages>231-242</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>The molecular diversity and community structure of bacteria from the gut of the termite
Reticulitermes speratus were analyzed by the sequencing of near-full-length 16S rRNA genes, amplified by polymerase chain reaction. The results of the analysis of 1344 clones indicated a predominance of spirochetes in the gut. Spirochetal clones accounted for approximately half of the analyzed clones. The clones related to
Bacteroides, Clostridia, and the candidate division Termite Group I each accounted for approximately 5–15% of the analyzed clones. The rest were comprised of Proteobacteria, Actinobacteria,
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| source | Oxford Journals Open Access Collection; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Actinobacteria Animal, plant and microbial ecology bacteria Bacteroides Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology clones Clostridium community structure digestive system Diversity Fundamental and applied biological sciences. Psychology genes Genetics Gut bacterium intestinal microorganisms Microbial ecology Mission oriented research monophyly Mycoplasma Normal microflora of man and animals. Rumen phylotype polymerase chain reaction Proteobacteria Reticulitermes speratus ribosomal RNA sequence analysis species diversity Spirochaetales Symbiosis Termite |
| title | Molecular analysis of bacterial microbiota in the gut of the termite Reticulitermes speratus (Isoptera; Rhinotermitidae) |
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