Purification, immobilization, and characterization of nattokinase on PHB nanoparticles
In this study, nattokinase was purified from Bacillus subtilis using ion exchange chromatography and immobilized upon polyhydroxybutyrate (PHB) nanoparticles. A novel strain isolated from industrial dairy waste was found to synthesize polyhydroxyalkanoates (PHA) and the strain was identified as Brev...
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| Veröffentlicht in: | Bioresource technology 2009-12, Vol.100 (24), p.6644-6646 |
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| creator | Deepak, Venkataraman Ram Kumar Pandian, Suresh babu Kalishwaralal, Kalimuthu Gurunathan, Sangiliyandi |
| description | In this study, nattokinase was purified from
Bacillus
subtilis using ion exchange chromatography and immobilized upon polyhydroxybutyrate (PHB) nanoparticles. A novel strain isolated from industrial dairy waste was found to synthesize polyhydroxyalkanoates (PHA) and the strain was identified as
Brevibacterium
casei SRKP2. PHA granules were extracted from 48
h culture and the FT-IR analysis characterized them as PHB, a natural biopolymer from
B.
casei. Nanoprecipitation by solvent displacement technique was used to synthesize PHB nanoparticles. PHB nanoparticles were characterized using transmission electron microscopy and particle size ranged from 100–125
nm. Immobilization of nattokinase upon PHB nanoparticles resulted in a 20% increase in the enzyme activity. Immobilization also contributed to the enhanced stability of the enzyme. Moreover, the activity was completely retained on storage at 4
°C for 25
days. The method has proven to be highly simple and can be implemented to other enzymes also. |
| doi_str_mv | 10.1016/j.biortech.2009.06.057 |
| format | Article |
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Bacillus
subtilis using ion exchange chromatography and immobilized upon polyhydroxybutyrate (PHB) nanoparticles. A novel strain isolated from industrial dairy waste was found to synthesize polyhydroxyalkanoates (PHA) and the strain was identified as
Brevibacterium
casei SRKP2. PHA granules were extracted from 48
h culture and the FT-IR analysis characterized them as PHB, a natural biopolymer from
B.
casei. Nanoprecipitation by solvent displacement technique was used to synthesize PHB nanoparticles. PHB nanoparticles were characterized using transmission electron microscopy and particle size ranged from 100–125
nm. Immobilization of nattokinase upon PHB nanoparticles resulted in a 20% increase in the enzyme activity. Immobilization also contributed to the enhanced stability of the enzyme. Moreover, the activity was completely retained on storage at 4
°C for 25
days. The method has proven to be highly simple and can be implemented to other enzymes also.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2009.06.057</identifier><identifier>PMID: 19608412</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Bacillus subtilis ; Bacillus subtilis - enzymology ; Biological and medical sciences ; Brevibacterium ; Electrophoresis, Polyacrylamide Gel ; Enzyme Stability ; Enzymes, Immobilized - metabolism ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Immobilization ; Nanoparticles - chemistry ; Nattokinase ; PHB nanoparticles ; Polyesters - chemistry ; Polyhydroxyalkanoates - chemistry ; Polyhydroxybutyrate ; Subtilisins - isolation & purification ; Temperature</subject><ispartof>Bioresource technology, 2009-12, Vol.100 (24), p.6644-6646</ispartof><rights>2009 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-467dbfff9bf8c814785b89b88defe47511e12d83d08c1f1d56b52890bde08edc3</citedby><cites>FETCH-LOGICAL-c452t-467dbfff9bf8c814785b89b88defe47511e12d83d08c1f1d56b52890bde08edc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2009.06.057$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21980887$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19608412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deepak, Venkataraman</creatorcontrib><creatorcontrib>Ram Kumar Pandian, Suresh babu</creatorcontrib><creatorcontrib>Kalishwaralal, Kalimuthu</creatorcontrib><creatorcontrib>Gurunathan, Sangiliyandi</creatorcontrib><title>Purification, immobilization, and characterization of nattokinase on PHB nanoparticles</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>In this study, nattokinase was purified from
Bacillus
subtilis using ion exchange chromatography and immobilized upon polyhydroxybutyrate (PHB) nanoparticles. A novel strain isolated from industrial dairy waste was found to synthesize polyhydroxyalkanoates (PHA) and the strain was identified as
Brevibacterium
casei SRKP2. PHA granules were extracted from 48
h culture and the FT-IR analysis characterized them as PHB, a natural biopolymer from
B.
casei. Nanoprecipitation by solvent displacement technique was used to synthesize PHB nanoparticles. PHB nanoparticles were characterized using transmission electron microscopy and particle size ranged from 100–125
nm. Immobilization of nattokinase upon PHB nanoparticles resulted in a 20% increase in the enzyme activity. Immobilization also contributed to the enhanced stability of the enzyme. Moreover, the activity was completely retained on storage at 4
°C for 25
days. The method has proven to be highly simple and can be implemented to other enzymes also.</description><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - enzymology</subject><subject>Biological and medical sciences</subject><subject>Brevibacterium</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme Stability</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immobilization</subject><subject>Nanoparticles - chemistry</subject><subject>Nattokinase</subject><subject>PHB nanoparticles</subject><subject>Polyesters - chemistry</subject><subject>Polyhydroxyalkanoates - chemistry</subject><subject>Polyhydroxybutyrate</subject><subject>Subtilisins - isolation & purification</subject><subject>Temperature</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi0EotvCK5RcoBcSxk7iTG5ABRSpEpWgXC3HHlMvSbzYWSR4erzaADc4Wf79zfjXx9g5h4oDly-21eBDXMjcVQKgr0BW0Hb32IZjV5ei7-R9toFeQomtaE7YaUpbAKh5Jx6yE54fsOFiwz7f7KN33ujFh_l54acpDH70P9e7nm1h7nTUZqG4pkVwxayXJXz1s05U5OTm6nWO5rDTcfFmpPSIPXB6TPR4Pc_Y7ds3ny6vyusP795fvrouTdOKpWxkZwfnXD84NMibDtsB-wHRkqOmazknLizWFtBwx20rh1ZgD4MlQLKmPmMXx727GL7tKS1q8snQOOqZwj6prm6A9x20mXz2T1IAcl4DZlAeQRNDSpGc2kU_6fhDcVAH92qrfrtXB_cKpMru8-D5-sN-mMj-HVtlZ-DpCuhk9Oiino1PfzjBewTEw6InR87poPSXmJnbjwJyOy4RpTh0fHkkKLv97imqZDzNhqyPZBZlg_9f21-9RbAV</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Deepak, Venkataraman</creator><creator>Ram Kumar Pandian, Suresh babu</creator><creator>Kalishwaralal, Kalimuthu</creator><creator>Gurunathan, Sangiliyandi</creator><general>Elsevier Ltd</general><general>[New York, NY]: Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20091201</creationdate><title>Purification, immobilization, and characterization of nattokinase on PHB nanoparticles</title><author>Deepak, Venkataraman ; Ram Kumar Pandian, Suresh babu ; Kalishwaralal, Kalimuthu ; Gurunathan, Sangiliyandi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-467dbfff9bf8c814785b89b88defe47511e12d83d08c1f1d56b52890bde08edc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - enzymology</topic><topic>Biological and medical sciences</topic><topic>Brevibacterium</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme Stability</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immobilization</topic><topic>Nanoparticles - chemistry</topic><topic>Nattokinase</topic><topic>PHB nanoparticles</topic><topic>Polyesters - chemistry</topic><topic>Polyhydroxyalkanoates - chemistry</topic><topic>Polyhydroxybutyrate</topic><topic>Subtilisins - isolation & purification</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deepak, Venkataraman</creatorcontrib><creatorcontrib>Ram Kumar Pandian, Suresh babu</creatorcontrib><creatorcontrib>Kalishwaralal, Kalimuthu</creatorcontrib><creatorcontrib>Gurunathan, Sangiliyandi</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deepak, Venkataraman</au><au>Ram Kumar Pandian, Suresh babu</au><au>Kalishwaralal, Kalimuthu</au><au>Gurunathan, Sangiliyandi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification, immobilization, and characterization of nattokinase on PHB nanoparticles</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>100</volume><issue>24</issue><spage>6644</spage><epage>6646</epage><pages>6644-6646</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>In this study, nattokinase was purified from
Bacillus
subtilis using ion exchange chromatography and immobilized upon polyhydroxybutyrate (PHB) nanoparticles. A novel strain isolated from industrial dairy waste was found to synthesize polyhydroxyalkanoates (PHA) and the strain was identified as
Brevibacterium
casei SRKP2. PHA granules were extracted from 48
h culture and the FT-IR analysis characterized them as PHB, a natural biopolymer from
B.
casei. Nanoprecipitation by solvent displacement technique was used to synthesize PHB nanoparticles. PHB nanoparticles were characterized using transmission electron microscopy and particle size ranged from 100–125
nm. Immobilization of nattokinase upon PHB nanoparticles resulted in a 20% increase in the enzyme activity. Immobilization also contributed to the enhanced stability of the enzyme. Moreover, the activity was completely retained on storage at 4
°C for 25
days. The method has proven to be highly simple and can be implemented to other enzymes also.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19608412</pmid><doi>10.1016/j.biortech.2009.06.057</doi><tpages>3</tpages></addata></record> |
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| subjects | Bacillus subtilis Bacillus subtilis - enzymology Biological and medical sciences Brevibacterium Electrophoresis, Polyacrylamide Gel Enzyme Stability Enzymes, Immobilized - metabolism Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Immobilization Nanoparticles - chemistry Nattokinase PHB nanoparticles Polyesters - chemistry Polyhydroxyalkanoates - chemistry Polyhydroxybutyrate Subtilisins - isolation & purification Temperature |
| title | Purification, immobilization, and characterization of nattokinase on PHB nanoparticles |
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