Distribution of the 3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived Actinobacteria
Abstract During the course of our screening program to isolate isoprenoids from marine Actinobacteria, 523 actinobacterial strains were isolated from 18 marine sponges, a tunicate, and two marine sediments. These strains belonged to 21 different genera, but most were members of Streptomyces, Nocardi...
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| Veröffentlicht in: | FEMS microbiology letters 2010-03, Vol.304 (1), p.89-96 |
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| creator | Khan, Shams Tabrez Izumikawa, Miho Motohashi, Keiichiro Mukai, Akira Takagi, Motoki Shin-Ya, Kazuo |
| description | Abstract
During the course of our screening program to isolate isoprenoids from marine Actinobacteria, 523 actinobacterial strains were isolated from 18 marine sponges, a tunicate, and two marine sediments. These strains belonged to 21 different genera, but most were members of Streptomyces, Nocardia, Rhodococcus, and Micromonospora. Some Actinobacteria have been reported to use the mevalonate pathway for the production of isoprenoids as secondary metabolites. Therefore, we investigated whether these strains possessed the 3-hydroxyl-3-methylglutaryl coenzyme A reductase (hmgr) gene, which indicates the presence of the mevalonate pathway. As a result, six strains belonging to the genera Streptomyces (SpC080624SC-11, SpA080624GE-02, and Sp080513GE-23), Nocardia (Sp080513SC-18), and Micromonospora (Se080624GE-07 and SpC080624GE-05) were found to possess the hmgr gene, and these genes were highly similar to hmgr genes in isoprenoid biosynthetic gene clusters. Among the six strains, the two strains SpC080624SC-11 and SpA080624GE-02 produced the novel isoprenoids, JBIR-46, -47, and -48, which consisted of phenazine chromophores, and Sp080513GE-23 produced a known isoprenoid, fumaquinone. Furthermore, these compounds showed cytotoxic activity against human acute myelogenous leukemia HL-60 cells. |
| doi_str_mv | 10.1111/j.1574-6968.2009.01886.x |
| format | Article |
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During the course of our screening program to isolate isoprenoids from marine Actinobacteria, 523 actinobacterial strains were isolated from 18 marine sponges, a tunicate, and two marine sediments. These strains belonged to 21 different genera, but most were members of Streptomyces, Nocardia, Rhodococcus, and Micromonospora. Some Actinobacteria have been reported to use the mevalonate pathway for the production of isoprenoids as secondary metabolites. Therefore, we investigated whether these strains possessed the 3-hydroxyl-3-methylglutaryl coenzyme A reductase (hmgr) gene, which indicates the presence of the mevalonate pathway. As a result, six strains belonging to the genera Streptomyces (SpC080624SC-11, SpA080624GE-02, and Sp080513GE-23), Nocardia (Sp080513SC-18), and Micromonospora (Se080624GE-07 and SpC080624GE-05) were found to possess the hmgr gene, and these genes were highly similar to hmgr genes in isoprenoid biosynthetic gene clusters. Among the six strains, the two strains SpC080624SC-11 and SpA080624GE-02 produced the novel isoprenoids, JBIR-46, -47, and -48, which consisted of phenazine chromophores, and Sp080513GE-23 produced a known isoprenoid, fumaquinone. Furthermore, these compounds showed cytotoxic activity against human acute myelogenous leukemia HL-60 cells.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2009.01886.x</identifier><identifier>PMID: 20067528</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>3‐hydroxy‐3‐methylglutaryl CoA reductases ; Actinobacteria ; Actinobacteria - classification ; Actinobacteria - enzymology ; Actinobacteria - genetics ; Actinobacteria - isolation & purification ; Acute myeloid leukemia ; Animal, plant and microbial ecology ; Animals ; Bacteriology ; Biological and medical sciences ; Chromophores ; Coenzyme A ; Cytotoxicity ; Fundamental and applied biological sciences. Psychology ; Gene clusters ; Genes ; Geologic Sediments - microbiology ; HL-60 Cells - drug effects ; Humans ; Hydroxymethylglutaryl CoA Reductases - genetics ; Hydroxymethylglutaryl CoA Reductases - metabolism ; Hydroxymethylglutaryl-CoA reductase ; isoprenoid ; Japan ; Leukemia ; marine Actinobacteria ; Marine Biology ; Marine sediments ; marine sponge ; Metabolism. Enzymes ; Metabolites ; Mevalonate pathway ; Mevalonic acid ; Microbial ecology ; Microbiology ; Micromonospora ; Molecular Sequence Data ; Myeloid leukemia ; Nocardia ; Phenazine ; Phylogeny ; Porifera - microbiology ; Reductases ; Rhodococcus ; Secondary metabolites ; Sediments ; Sequence Analysis, DNA ; Statins ; Streptomyces ; Terpenes ; Terpenes - chemistry ; Terpenes - metabolism ; Terpenes - pharmacology ; Urochordata - microbiology ; Various environments (extraatmospheric space, air, water)</subject><ispartof>FEMS microbiology letters, 2010-03, Vol.304 (1), p.89-96</ispartof><rights>2009 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved 2009</rights><rights>2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2015 INIST-CNRS</rights><rights>2009 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5996-d8e1f63666f55932ca1e5ee8a4e8a2b604a555f35d747ed420b1c64b357d55063</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2009.01886.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2009.01886.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22363485$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20067528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Shams Tabrez</creatorcontrib><creatorcontrib>Izumikawa, Miho</creatorcontrib><creatorcontrib>Motohashi, Keiichiro</creatorcontrib><creatorcontrib>Mukai, Akira</creatorcontrib><creatorcontrib>Takagi, Motoki</creatorcontrib><creatorcontrib>Shin-Ya, Kazuo</creatorcontrib><title>Distribution of the 3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived Actinobacteria</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
During the course of our screening program to isolate isoprenoids from marine Actinobacteria, 523 actinobacterial strains were isolated from 18 marine sponges, a tunicate, and two marine sediments. These strains belonged to 21 different genera, but most were members of Streptomyces, Nocardia, Rhodococcus, and Micromonospora. Some Actinobacteria have been reported to use the mevalonate pathway for the production of isoprenoids as secondary metabolites. Therefore, we investigated whether these strains possessed the 3-hydroxyl-3-methylglutaryl coenzyme A reductase (hmgr) gene, which indicates the presence of the mevalonate pathway. As a result, six strains belonging to the genera Streptomyces (SpC080624SC-11, SpA080624GE-02, and Sp080513GE-23), Nocardia (Sp080513SC-18), and Micromonospora (Se080624GE-07 and SpC080624GE-05) were found to possess the hmgr gene, and these genes were highly similar to hmgr genes in isoprenoid biosynthetic gene clusters. Among the six strains, the two strains SpC080624SC-11 and SpA080624GE-02 produced the novel isoprenoids, JBIR-46, -47, and -48, which consisted of phenazine chromophores, and Sp080513GE-23 produced a known isoprenoid, fumaquinone. Furthermore, these compounds showed cytotoxic activity against human acute myelogenous leukemia HL-60 cells.</description><subject>3‐hydroxy‐3‐methylglutaryl CoA reductases</subject><subject>Actinobacteria</subject><subject>Actinobacteria - classification</subject><subject>Actinobacteria - enzymology</subject><subject>Actinobacteria - genetics</subject><subject>Actinobacteria - isolation & purification</subject><subject>Acute myeloid leukemia</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chromophores</subject><subject>Coenzyme A</subject><subject>Cytotoxicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene clusters</subject><subject>Genes</subject><subject>Geologic Sediments - microbiology</subject><subject>HL-60 Cells - drug effects</subject><subject>Humans</subject><subject>Hydroxymethylglutaryl CoA Reductases - genetics</subject><subject>Hydroxymethylglutaryl CoA Reductases - metabolism</subject><subject>Hydroxymethylglutaryl-CoA reductase</subject><subject>isoprenoid</subject><subject>Japan</subject><subject>Leukemia</subject><subject>marine Actinobacteria</subject><subject>Marine Biology</subject><subject>Marine sediments</subject><subject>marine sponge</subject><subject>Metabolism. Enzymes</subject><subject>Metabolites</subject><subject>Mevalonate pathway</subject><subject>Mevalonic acid</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Micromonospora</subject><subject>Molecular Sequence Data</subject><subject>Myeloid leukemia</subject><subject>Nocardia</subject><subject>Phenazine</subject><subject>Phylogeny</subject><subject>Porifera - microbiology</subject><subject>Reductases</subject><subject>Rhodococcus</subject><subject>Secondary metabolites</subject><subject>Sediments</subject><subject>Sequence Analysis, DNA</subject><subject>Statins</subject><subject>Streptomyces</subject><subject>Terpenes</subject><subject>Terpenes - chemistry</subject><subject>Terpenes - metabolism</subject><subject>Terpenes - pharmacology</subject><subject>Urochordata - microbiology</subject><subject>Various environments (extraatmospheric space, air, water)</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl-P1CAUxRujccfVr2BIjPrUCqX86YMPk3VXTcb4os-EltsdJi2M0K5Tn_eDS51xTTQaSQiE8zsnF7hZhgguSBqvdgVhosp5zWVRYlwXmEjJi8O9bHUn3M9WmAqZE1yLs-xRjDuMcVVi_jA7Sx4uWClX2e0bG8dgm2m03iHfoXELiObb2QR_mPuc5gOM27m_7qdRh7lHrQf3bR4ArVEAM7WjjoCuwQHSziAb_T6A89agffCLvMRahwYdrIPcQLA3YNA6Cc43uh3TgX6cPeh0H-HJaT3PPl9dfrp4l28-vn1_sd7kLatrnhsJpOOUc94xVtOy1QQYgNRVmmXDcaUZYx1lRlQCTLprQ1peNZQJwxjm9Dx7ecxNtX2ZII5qsLGFvtcO_BSVoBXGUooqkS_-SZaE4ZLI-j_AqqaixAl89hu481Nw6bqqpKk2UguyxD09UVMzgFH7YNPLzernhyXg-QnQsdV9F7RrbfzFlZTTSrLEvT5yX20P851OsFoaSO3U0idq6ZMlu1Y_Gkgd1NWHzbJLfnr0-2n_F3f-h5t-B2lzyEk</recordid><startdate>201003</startdate><enddate>201003</enddate><creator>Khan, Shams Tabrez</creator><creator>Izumikawa, Miho</creator><creator>Motohashi, Keiichiro</creator><creator>Mukai, Akira</creator><creator>Takagi, Motoki</creator><creator>Shin-Ya, Kazuo</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>201003</creationdate><title>Distribution of the 3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived Actinobacteria</title><author>Khan, Shams Tabrez ; Izumikawa, Miho ; Motohashi, Keiichiro ; Mukai, Akira ; Takagi, Motoki ; Shin-Ya, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5996-d8e1f63666f55932ca1e5ee8a4e8a2b604a555f35d747ed420b1c64b357d55063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>3‐hydroxy‐3‐methylglutaryl CoA reductases</topic><topic>Actinobacteria</topic><topic>Actinobacteria - classification</topic><topic>Actinobacteria - enzymology</topic><topic>Actinobacteria - genetics</topic><topic>Actinobacteria - isolation & purification</topic><topic>Acute myeloid leukemia</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chromophores</topic><topic>Coenzyme A</topic><topic>Cytotoxicity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene clusters</topic><topic>Genes</topic><topic>Geologic Sediments - microbiology</topic><topic>HL-60 Cells - drug effects</topic><topic>Humans</topic><topic>Hydroxymethylglutaryl CoA Reductases - genetics</topic><topic>Hydroxymethylglutaryl CoA Reductases - metabolism</topic><topic>Hydroxymethylglutaryl-CoA reductase</topic><topic>isoprenoid</topic><topic>Japan</topic><topic>Leukemia</topic><topic>marine Actinobacteria</topic><topic>Marine Biology</topic><topic>Marine sediments</topic><topic>marine sponge</topic><topic>Metabolism. Enzymes</topic><topic>Metabolites</topic><topic>Mevalonate pathway</topic><topic>Mevalonic acid</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Micromonospora</topic><topic>Molecular Sequence Data</topic><topic>Myeloid leukemia</topic><topic>Nocardia</topic><topic>Phenazine</topic><topic>Phylogeny</topic><topic>Porifera - microbiology</topic><topic>Reductases</topic><topic>Rhodococcus</topic><topic>Secondary metabolites</topic><topic>Sediments</topic><topic>Sequence Analysis, DNA</topic><topic>Statins</topic><topic>Streptomyces</topic><topic>Terpenes</topic><topic>Terpenes - chemistry</topic><topic>Terpenes - metabolism</topic><topic>Terpenes - pharmacology</topic><topic>Urochordata - microbiology</topic><topic>Various environments (extraatmospheric space, air, water)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Shams Tabrez</creatorcontrib><creatorcontrib>Izumikawa, Miho</creatorcontrib><creatorcontrib>Motohashi, Keiichiro</creatorcontrib><creatorcontrib>Mukai, Akira</creatorcontrib><creatorcontrib>Takagi, Motoki</creatorcontrib><creatorcontrib>Shin-Ya, Kazuo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Shams Tabrez</au><au>Izumikawa, Miho</au><au>Motohashi, Keiichiro</au><au>Mukai, Akira</au><au>Takagi, Motoki</au><au>Shin-Ya, Kazuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of the 3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived Actinobacteria</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2010-03</date><risdate>2010</risdate><volume>304</volume><issue>1</issue><spage>89</spage><epage>96</epage><pages>89-96</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
During the course of our screening program to isolate isoprenoids from marine Actinobacteria, 523 actinobacterial strains were isolated from 18 marine sponges, a tunicate, and two marine sediments. These strains belonged to 21 different genera, but most were members of Streptomyces, Nocardia, Rhodococcus, and Micromonospora. Some Actinobacteria have been reported to use the mevalonate pathway for the production of isoprenoids as secondary metabolites. Therefore, we investigated whether these strains possessed the 3-hydroxyl-3-methylglutaryl coenzyme A reductase (hmgr) gene, which indicates the presence of the mevalonate pathway. As a result, six strains belonging to the genera Streptomyces (SpC080624SC-11, SpA080624GE-02, and Sp080513GE-23), Nocardia (Sp080513SC-18), and Micromonospora (Se080624GE-07 and SpC080624GE-05) were found to possess the hmgr gene, and these genes were highly similar to hmgr genes in isoprenoid biosynthetic gene clusters. Among the six strains, the two strains SpC080624SC-11 and SpA080624GE-02 produced the novel isoprenoids, JBIR-46, -47, and -48, which consisted of phenazine chromophores, and Sp080513GE-23 produced a known isoprenoid, fumaquinone. Furthermore, these compounds showed cytotoxic activity against human acute myelogenous leukemia HL-60 cells.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20067528</pmid><doi>10.1111/j.1574-6968.2009.01886.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3‐hydroxy‐3‐methylglutaryl CoA reductases Actinobacteria Actinobacteria - classification Actinobacteria - enzymology Actinobacteria - genetics Actinobacteria - isolation & purification Acute myeloid leukemia Animal, plant and microbial ecology Animals Bacteriology Biological and medical sciences Chromophores Coenzyme A Cytotoxicity Fundamental and applied biological sciences. Psychology Gene clusters Genes Geologic Sediments - microbiology HL-60 Cells - drug effects Humans Hydroxymethylglutaryl CoA Reductases - genetics Hydroxymethylglutaryl CoA Reductases - metabolism Hydroxymethylglutaryl-CoA reductase isoprenoid Japan Leukemia marine Actinobacteria Marine Biology Marine sediments marine sponge Metabolism. Enzymes Metabolites Mevalonate pathway Mevalonic acid Microbial ecology Microbiology Micromonospora Molecular Sequence Data Myeloid leukemia Nocardia Phenazine Phylogeny Porifera - microbiology Reductases Rhodococcus Secondary metabolites Sediments Sequence Analysis, DNA Statins Streptomyces Terpenes Terpenes - chemistry Terpenes - metabolism Terpenes - pharmacology Urochordata - microbiology Various environments (extraatmospheric space, air, water) |
| title | Distribution of the 3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived Actinobacteria |
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