Distribution of the 3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived Actinobacteria

Abstract During the course of our screening program to isolate isoprenoids from marine Actinobacteria, 523 actinobacterial strains were isolated from 18 marine sponges, a tunicate, and two marine sediments. These strains belonged to 21 different genera, but most were members of Streptomyces, Nocardi...

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Veröffentlicht in:FEMS microbiology letters 2010-03, Vol.304 (1), p.89-96
Hauptverfasser: Khan, Shams Tabrez, Izumikawa, Miho, Motohashi, Keiichiro, Mukai, Akira, Takagi, Motoki, Shin-Ya, Kazuo
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container_title FEMS microbiology letters
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creator Khan, Shams Tabrez
Izumikawa, Miho
Motohashi, Keiichiro
Mukai, Akira
Takagi, Motoki
Shin-Ya, Kazuo
description Abstract During the course of our screening program to isolate isoprenoids from marine Actinobacteria, 523 actinobacterial strains were isolated from 18 marine sponges, a tunicate, and two marine sediments. These strains belonged to 21 different genera, but most were members of Streptomyces, Nocardia, Rhodococcus, and Micromonospora. Some Actinobacteria have been reported to use the mevalonate pathway for the production of isoprenoids as secondary metabolites. Therefore, we investigated whether these strains possessed the 3-hydroxyl-3-methylglutaryl coenzyme A reductase (hmgr) gene, which indicates the presence of the mevalonate pathway. As a result, six strains belonging to the genera Streptomyces (SpC080624SC-11, SpA080624GE-02, and Sp080513GE-23), Nocardia (Sp080513SC-18), and Micromonospora (Se080624GE-07 and SpC080624GE-05) were found to possess the hmgr gene, and these genes were highly similar to hmgr genes in isoprenoid biosynthetic gene clusters. Among the six strains, the two strains SpC080624SC-11 and SpA080624GE-02 produced the novel isoprenoids, JBIR-46, -47, and -48, which consisted of phenazine chromophores, and Sp080513GE-23 produced a known isoprenoid, fumaquinone. Furthermore, these compounds showed cytotoxic activity against human acute myelogenous leukemia HL-60 cells.
doi_str_mv 10.1111/j.1574-6968.2009.01886.x
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These strains belonged to 21 different genera, but most were members of Streptomyces, Nocardia, Rhodococcus, and Micromonospora. Some Actinobacteria have been reported to use the mevalonate pathway for the production of isoprenoids as secondary metabolites. Therefore, we investigated whether these strains possessed the 3-hydroxyl-3-methylglutaryl coenzyme A reductase (hmgr) gene, which indicates the presence of the mevalonate pathway. As a result, six strains belonging to the genera Streptomyces (SpC080624SC-11, SpA080624GE-02, and Sp080513GE-23), Nocardia (Sp080513SC-18), and Micromonospora (Se080624GE-07 and SpC080624GE-05) were found to possess the hmgr gene, and these genes were highly similar to hmgr genes in isoprenoid biosynthetic gene clusters. Among the six strains, the two strains SpC080624SC-11 and SpA080624GE-02 produced the novel isoprenoids, JBIR-46, -47, and -48, which consisted of phenazine chromophores, and Sp080513GE-23 produced a known isoprenoid, fumaquinone. 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Psychology ; Gene clusters ; Genes ; Geologic Sediments - microbiology ; HL-60 Cells - drug effects ; Humans ; Hydroxymethylglutaryl CoA Reductases - genetics ; Hydroxymethylglutaryl CoA Reductases - metabolism ; Hydroxymethylglutaryl-CoA reductase ; isoprenoid ; Japan ; Leukemia ; marine Actinobacteria ; Marine Biology ; Marine sediments ; marine sponge ; Metabolism. Enzymes ; Metabolites ; Mevalonate pathway ; Mevalonic acid ; Microbial ecology ; Microbiology ; Micromonospora ; Molecular Sequence Data ; Myeloid leukemia ; Nocardia ; Phenazine ; Phylogeny ; Porifera - microbiology ; Reductases ; Rhodococcus ; Secondary metabolites ; Sediments ; Sequence Analysis, DNA ; Statins ; Streptomyces ; Terpenes ; Terpenes - chemistry ; Terpenes - metabolism ; Terpenes - pharmacology ; Urochordata - microbiology ; Various environments (extraatmospheric space, air, water)</subject><ispartof>FEMS microbiology letters, 2010-03, Vol.304 (1), p.89-96</ispartof><rights>2009 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved 2009</rights><rights>2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2015 INIST-CNRS</rights><rights>2009 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. 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These strains belonged to 21 different genera, but most were members of Streptomyces, Nocardia, Rhodococcus, and Micromonospora. Some Actinobacteria have been reported to use the mevalonate pathway for the production of isoprenoids as secondary metabolites. Therefore, we investigated whether these strains possessed the 3-hydroxyl-3-methylglutaryl coenzyme A reductase (hmgr) gene, which indicates the presence of the mevalonate pathway. As a result, six strains belonging to the genera Streptomyces (SpC080624SC-11, SpA080624GE-02, and Sp080513GE-23), Nocardia (Sp080513SC-18), and Micromonospora (Se080624GE-07 and SpC080624GE-05) were found to possess the hmgr gene, and these genes were highly similar to hmgr genes in isoprenoid biosynthetic gene clusters. Among the six strains, the two strains SpC080624SC-11 and SpA080624GE-02 produced the novel isoprenoids, JBIR-46, -47, and -48, which consisted of phenazine chromophores, and Sp080513GE-23 produced a known isoprenoid, fumaquinone. Furthermore, these compounds showed cytotoxic activity against human acute myelogenous leukemia HL-60 cells.</description><subject>3‐hydroxy‐3‐methylglutaryl CoA reductases</subject><subject>Actinobacteria</subject><subject>Actinobacteria - classification</subject><subject>Actinobacteria - enzymology</subject><subject>Actinobacteria - genetics</subject><subject>Actinobacteria - isolation &amp; purification</subject><subject>Acute myeloid leukemia</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chromophores</subject><subject>Coenzyme A</subject><subject>Cytotoxicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene clusters</subject><subject>Genes</subject><subject>Geologic Sediments - microbiology</subject><subject>HL-60 Cells - drug effects</subject><subject>Humans</subject><subject>Hydroxymethylglutaryl CoA Reductases - genetics</subject><subject>Hydroxymethylglutaryl CoA Reductases - metabolism</subject><subject>Hydroxymethylglutaryl-CoA reductase</subject><subject>isoprenoid</subject><subject>Japan</subject><subject>Leukemia</subject><subject>marine Actinobacteria</subject><subject>Marine Biology</subject><subject>Marine sediments</subject><subject>marine sponge</subject><subject>Metabolism. Enzymes</subject><subject>Metabolites</subject><subject>Mevalonate pathway</subject><subject>Mevalonic acid</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Micromonospora</subject><subject>Molecular Sequence Data</subject><subject>Myeloid leukemia</subject><subject>Nocardia</subject><subject>Phenazine</subject><subject>Phylogeny</subject><subject>Porifera - microbiology</subject><subject>Reductases</subject><subject>Rhodococcus</subject><subject>Secondary metabolites</subject><subject>Sediments</subject><subject>Sequence Analysis, DNA</subject><subject>Statins</subject><subject>Streptomyces</subject><subject>Terpenes</subject><subject>Terpenes - chemistry</subject><subject>Terpenes - metabolism</subject><subject>Terpenes - pharmacology</subject><subject>Urochordata - microbiology</subject><subject>Various environments (extraatmospheric space, air, water)</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl-P1CAUxRujccfVr2BIjPrUCqX86YMPk3VXTcb4os-EltsdJi2M0K5Tn_eDS51xTTQaSQiE8zsnF7hZhgguSBqvdgVhosp5zWVRYlwXmEjJi8O9bHUn3M9WmAqZE1yLs-xRjDuMcVVi_jA7Sx4uWClX2e0bG8dgm2m03iHfoXELiObb2QR_mPuc5gOM27m_7qdRh7lHrQf3bR4ArVEAM7WjjoCuwQHSziAb_T6A89agffCLvMRahwYdrIPcQLA3YNA6Cc43uh3TgX6cPeh0H-HJaT3PPl9dfrp4l28-vn1_sd7kLatrnhsJpOOUc94xVtOy1QQYgNRVmmXDcaUZYx1lRlQCTLprQ1peNZQJwxjm9Dx7ecxNtX2ZII5qsLGFvtcO_BSVoBXGUooqkS_-SZaE4ZLI-j_AqqaixAl89hu481Nw6bqqpKk2UguyxD09UVMzgFH7YNPLzernhyXg-QnQsdV9F7RrbfzFlZTTSrLEvT5yX20P851OsFoaSO3U0idq6ZMlu1Y_Gkgd1NWHzbJLfnr0-2n_F3f-h5t-B2lzyEk</recordid><startdate>201003</startdate><enddate>201003</enddate><creator>Khan, Shams Tabrez</creator><creator>Izumikawa, Miho</creator><creator>Motohashi, Keiichiro</creator><creator>Mukai, Akira</creator><creator>Takagi, Motoki</creator><creator>Shin-Ya, Kazuo</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>201003</creationdate><title>Distribution of the 3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived Actinobacteria</title><author>Khan, Shams Tabrez ; Izumikawa, Miho ; Motohashi, Keiichiro ; Mukai, Akira ; Takagi, Motoki ; Shin-Ya, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5996-d8e1f63666f55932ca1e5ee8a4e8a2b604a555f35d747ed420b1c64b357d55063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>3‐hydroxy‐3‐methylglutaryl CoA reductases</topic><topic>Actinobacteria</topic><topic>Actinobacteria - classification</topic><topic>Actinobacteria - enzymology</topic><topic>Actinobacteria - genetics</topic><topic>Actinobacteria - isolation &amp; purification</topic><topic>Acute myeloid leukemia</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chromophores</topic><topic>Coenzyme A</topic><topic>Cytotoxicity</topic><topic>Fundamental and applied biological sciences. 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source MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current)
subjects 3‐hydroxy‐3‐methylglutaryl CoA reductases
Actinobacteria
Actinobacteria - classification
Actinobacteria - enzymology
Actinobacteria - genetics
Actinobacteria - isolation & purification
Acute myeloid leukemia
Animal, plant and microbial ecology
Animals
Bacteriology
Biological and medical sciences
Chromophores
Coenzyme A
Cytotoxicity
Fundamental and applied biological sciences. Psychology
Gene clusters
Genes
Geologic Sediments - microbiology
HL-60 Cells - drug effects
Humans
Hydroxymethylglutaryl CoA Reductases - genetics
Hydroxymethylglutaryl CoA Reductases - metabolism
Hydroxymethylglutaryl-CoA reductase
isoprenoid
Japan
Leukemia
marine Actinobacteria
Marine Biology
Marine sediments
marine sponge
Metabolism. Enzymes
Metabolites
Mevalonate pathway
Mevalonic acid
Microbial ecology
Microbiology
Micromonospora
Molecular Sequence Data
Myeloid leukemia
Nocardia
Phenazine
Phylogeny
Porifera - microbiology
Reductases
Rhodococcus
Secondary metabolites
Sediments
Sequence Analysis, DNA
Statins
Streptomyces
Terpenes
Terpenes - chemistry
Terpenes - metabolism
Terpenes - pharmacology
Urochordata - microbiology
Various environments (extraatmospheric space, air, water)
title Distribution of the 3-hydroxyl-3-methylglutaryl coenzyme A reductase gene and isoprenoid production in marine-derived Actinobacteria
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