P2X₇ pre-synaptic receptors in adult rat cerebrocortical nerve terminals: a role in ATP-induced glutamate release
Although growing evidence suggests that extracellular ATP might play roles in the control of astrocyte/neuron crosstalk in the CNS by acting on P2X₇ receptors, it is still unclear whether neuronal functions can be attributed to P2X₇ receptors. In the present paper, we investigate the location, pharm...
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| Veröffentlicht in: | Journal of neurochemistry 2008-06, Vol.105 (6), p.2330-2342 |
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| creator | Marcoli, Manuela Cervetto, Chiara Paluzzi, Paola Guarnieri, Stefania Alloisio, Susanna Thellung, Stefano Nobile, Mario Maura, Guido |
| description | Although growing evidence suggests that extracellular ATP might play roles in the control of astrocyte/neuron crosstalk in the CNS by acting on P2X₇ receptors, it is still unclear whether neuronal functions can be attributed to P2X₇ receptors. In the present paper, we investigate the location, pharmacological profile, and function of P2X₇ receptors on cerebrocortical nerve terminals freshly prepared from adult rats, by measuring glutamate release and calcium accumulation. The preparation chosen (purified synaptosomes) ensures negligible contamination of non-neuronal cells and allows exposure of 'nude' release-regulating pre-synaptic receptors. To confirm the results obtained, we also carried out specific experiments on human embryonic kidney 293 cells which had been stably transfected with rat P2X₇ receptors. Together, our findings suggest that (i) P2X₇ receptors are present in a subpopulation of adult rat cerebrocortical nerve terminals; (ii) P2X₇ receptors are localized on glutamatergic nerve terminals; (iii) P2X₇ receptors play a significant role in ATP-evoked glutamate efflux, which involves Ca²⁺-dependent vesicular release; and (iv) the P2X₇ receptor itself constitutes a significant Ca²⁺-independent mode of exit for glutamate. |
| doi_str_mv | 10.1111/j.1471-4159.2008.05322.x |
| format | Article |
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In the present paper, we investigate the location, pharmacological profile, and function of P2X₇ receptors on cerebrocortical nerve terminals freshly prepared from adult rats, by measuring glutamate release and calcium accumulation. The preparation chosen (purified synaptosomes) ensures negligible contamination of non-neuronal cells and allows exposure of 'nude' release-regulating pre-synaptic receptors. To confirm the results obtained, we also carried out specific experiments on human embryonic kidney 293 cells which had been stably transfected with rat P2X₇ receptors. Together, our findings suggest that (i) P2X₇ receptors are present in a subpopulation of adult rat cerebrocortical nerve terminals; (ii) P2X₇ receptors are localized on glutamatergic nerve terminals; (iii) P2X₇ receptors play a significant role in ATP-evoked glutamate efflux, which involves Ca²⁺-dependent vesicular release; and (iv) the P2X₇ receptor itself constitutes a significant Ca²⁺-independent mode of exit for glutamate.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.2008.05322.x</identifier><identifier>PMID: 18315565</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Adenosine triphosphatase ; Adenosine Triphosphate - physiology ; Age Factors ; Animals ; Biochemistry ; Biological and medical sciences ; Brain ; Cell Line ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Cerebral Cortex - secretion ; cerebrocortical synaptosomes ; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases ; Fundamental and applied biological sciences. Psychology ; glutamate release ; Glutamic Acid - secretion ; human embryonic kidney 293 cells ; Humans ; Male ; Medical sciences ; Neurology ; P2X7 receptors ; P2X₇ receptors ; Presynaptic Terminals - secretion ; Rats ; Rats, Sprague-Dawley ; Receptors, Purinergic P2 - physiology ; Receptors, Purinergic P2X7 ; Rodents ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neurochemistry, 2008-06, Vol.105 (6), p.2330-2342</ispartof><rights>2008 The Authors. 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Together, our findings suggest that (i) P2X₇ receptors are present in a subpopulation of adult rat cerebrocortical nerve terminals; (ii) P2X₇ receptors are localized on glutamatergic nerve terminals; (iii) P2X₇ receptors play a significant role in ATP-evoked glutamate efflux, which involves Ca²⁺-dependent vesicular release; and (iv) the P2X₇ receptor itself constitutes a significant Ca²⁺-independent mode of exit for glutamate.</description><subject>Adenosine triphosphatase</subject><subject>Adenosine Triphosphate - physiology</subject><subject>Age Factors</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Brain</subject><subject>Cell Line</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Cerebral Cortex - secretion</subject><subject>cerebrocortical synaptosomes</subject><subject>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>glutamate release</subject><subject>Glutamic Acid - secretion</subject><subject>human embryonic kidney 293 cells</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neurology</subject><subject>P2X7 receptors</subject><subject>P2X₇ receptors</subject><subject>Presynaptic Terminals - secretion</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Purinergic P2 - physiology</subject><subject>Receptors, Purinergic P2X7</subject><subject>Rodents</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkcuKFTEQhoMoznH0FTQI4qrbyq3TLbgYDl4ZdMAZcBfSSfXQh76ZdOucrRvf0ycx7TmOYDYJ1Pf_laqfEMogZ-m82OVMapZJpqqcA5Q5KMF5fnOHbG4Ld8kGgPNMgOQn5EGMOwBWyILdJyesFEypQm1IvOBffv34SaeAWdwPdppbRwM6nOYxRNoO1Pqlm2mwM3UYsA6jG0OCbEcHDN-Qzhj6drBdfEktDWOHq-js8iJrB7849PS6W2bb2xmTb4c24kNyr0k8Pjrep-TqzevL7bvs_NPb99uz86wRuuAZCl06J5m3lXZcSe1E7Z1Q6MsijVIht02qlHUNWFaKMVAaUSjvpfS-4OKUPD_4TmH8umCcTd9Gh11nBxyXaLSQAFqxKpFP_yN34xLWoQyHQilWMJ2gx0doqXv0Zgptb8Pe_F1mAp4dARvTfppgB9fGW46DBMXL9V-vDtz3tsP9Px8wa7hmZ9YMzZqhWcM1f8I1N-bDx-36SvonB31jR2OvQ-px9ZkDEwAVcKiU-A0UsaDk</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Marcoli, Manuela</creator><creator>Cervetto, Chiara</creator><creator>Paluzzi, Paola</creator><creator>Guarnieri, Stefania</creator><creator>Alloisio, Susanna</creator><creator>Thellung, Stefano</creator><creator>Nobile, Mario</creator><creator>Maura, Guido</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200806</creationdate><title>P2X₇ pre-synaptic receptors in adult rat cerebrocortical nerve terminals: a role in ATP-induced glutamate release</title><author>Marcoli, Manuela ; Cervetto, Chiara ; Paluzzi, Paola ; Guarnieri, Stefania ; Alloisio, Susanna ; Thellung, Stefano ; Nobile, Mario ; Maura, Guido</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f3762-e378cc41da97c2547c3bdc35ed860019e2af97c8bb0e89511057ee35dd44dd623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adenosine triphosphatase</topic><topic>Adenosine Triphosphate - physiology</topic><topic>Age Factors</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Brain</topic><topic>Cell Line</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Cerebral Cortex - secretion</topic><topic>cerebrocortical synaptosomes</topic><topic>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>glutamate release</topic><topic>Glutamic Acid - secretion</topic><topic>human embryonic kidney 293 cells</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neurology</topic><topic>P2X7 receptors</topic><topic>P2X₇ receptors</topic><topic>Presynaptic Terminals - secretion</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Purinergic P2 - physiology</topic><topic>Receptors, Purinergic P2X7</topic><topic>Rodents</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marcoli, Manuela</creatorcontrib><creatorcontrib>Cervetto, Chiara</creatorcontrib><creatorcontrib>Paluzzi, Paola</creatorcontrib><creatorcontrib>Guarnieri, Stefania</creatorcontrib><creatorcontrib>Alloisio, Susanna</creatorcontrib><creatorcontrib>Thellung, Stefano</creatorcontrib><creatorcontrib>Nobile, Mario</creatorcontrib><creatorcontrib>Maura, Guido</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marcoli, Manuela</au><au>Cervetto, Chiara</au><au>Paluzzi, Paola</au><au>Guarnieri, Stefania</au><au>Alloisio, Susanna</au><au>Thellung, Stefano</au><au>Nobile, Mario</au><au>Maura, Guido</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>P2X₇ pre-synaptic receptors in adult rat cerebrocortical nerve terminals: a role in ATP-induced glutamate release</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>2008-06</date><risdate>2008</risdate><volume>105</volume><issue>6</issue><spage>2330</spage><epage>2342</epage><pages>2330-2342</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>Although growing evidence suggests that extracellular ATP might play roles in the control of astrocyte/neuron crosstalk in the CNS by acting on P2X₇ receptors, it is still unclear whether neuronal functions can be attributed to P2X₇ receptors. In the present paper, we investigate the location, pharmacological profile, and function of P2X₇ receptors on cerebrocortical nerve terminals freshly prepared from adult rats, by measuring glutamate release and calcium accumulation. The preparation chosen (purified synaptosomes) ensures negligible contamination of non-neuronal cells and allows exposure of 'nude' release-regulating pre-synaptic receptors. To confirm the results obtained, we also carried out specific experiments on human embryonic kidney 293 cells which had been stably transfected with rat P2X₇ receptors. Together, our findings suggest that (i) P2X₇ receptors are present in a subpopulation of adult rat cerebrocortical nerve terminals; (ii) P2X₇ receptors are localized on glutamatergic nerve terminals; (iii) P2X₇ receptors play a significant role in ATP-evoked glutamate efflux, which involves Ca²⁺-dependent vesicular release; and (iv) the P2X₇ receptor itself constitutes a significant Ca²⁺-independent mode of exit for glutamate.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18315565</pmid><doi>10.1111/j.1471-4159.2008.05322.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenosine triphosphatase Adenosine Triphosphate - physiology Age Factors Animals Biochemistry Biological and medical sciences Brain Cell Line Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Cerebral Cortex - secretion cerebrocortical synaptosomes Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Fundamental and applied biological sciences. Psychology glutamate release Glutamic Acid - secretion human embryonic kidney 293 cells Humans Male Medical sciences Neurology P2X7 receptors P2X₇ receptors Presynaptic Terminals - secretion Rats Rats, Sprague-Dawley Receptors, Purinergic P2 - physiology Receptors, Purinergic P2X7 Rodents Vertebrates: nervous system and sense organs |
| title | P2X₇ pre-synaptic receptors in adult rat cerebrocortical nerve terminals: a role in ATP-induced glutamate release |
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