Diclofenac inhibits proliferation and differentiation of neural stem cells
Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used in clinical situations as anti-inflammatory, analgesic and antipyretic drugs. However, it is still unknown whether NSAIDs have effects on the development of the central nervous system. In the present study, we investigated the effects of...
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| Veröffentlicht in: | Biochemical pharmacology 2003-07, Vol.66 (2), p.289-295 |
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| container_title | Biochemical pharmacology |
| container_volume | 66 |
| creator | Kudo, Chiho Kori, Maya Matsuzaki, Kiyomi Yamai, Kenshiki Nakajima, Atsushi Shibuya, Atsuhito Niwa, Hitoshi Kamisaki, Yoshinori Wada, Koichiro |
| description | Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used in clinical situations as anti-inflammatory, analgesic and antipyretic drugs. However, it is still unknown whether NSAIDs have effects on the development of the central nervous system. In the present study, we investigated the effects of NSAIDs on neural stem cell (NSC) proliferation and differentiation into neurons. In contrast to aspirin, naproxen, indomethacin and ibuprofen, treatment with diclofenac (10
μM) for 2 days induced the death of NSCs in a concentration-dependent manner. Diclofenac also inhibited the proliferation of NSCs and their differentiation into neurons. Treatment with diclofenac resulted in nuclear condensation (a morphological change due to apoptosis of NSCs) 24
hr after the treatment and activated caspase-3 after 6
hr, indicating that diclofenac may cause apoptosis of neuronal cells
via activation of the caspase cascade. These results suggest that diclofenac may affect the development of the central nervous system. |
| doi_str_mv | 10.1016/S0006-2952(03)00235-1 |
| format | Article |
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μM) for 2 days induced the death of NSCs in a concentration-dependent manner. Diclofenac also inhibited the proliferation of NSCs and their differentiation into neurons. Treatment with diclofenac resulted in nuclear condensation (a morphological change due to apoptosis of NSCs) 24
hr after the treatment and activated caspase-3 after 6
hr, indicating that diclofenac may cause apoptosis of neuronal cells
via activation of the caspase cascade. These results suggest that diclofenac may affect the development of the central nervous system.</description><identifier>ISSN: 0006-2952</identifier><identifier>EISSN: 1873-2968</identifier><identifier>DOI: 10.1016/S0006-2952(03)00235-1</identifier><identifier>PMID: 12826271</identifier><identifier>CODEN: BCPCA6</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Apoptosis ; Biological and medical sciences ; Caspases - metabolism ; Cell Differentiation - drug effects ; Cell Division - drug effects ; Central nervous system ; Diclofenac ; Diclofenac - pharmacology ; Enzyme Activation ; Medical sciences ; Mice ; Neural stem cells ; Neurons - cytology ; Neurons - drug effects ; Nonsteroidal anti-inflammatory drugs ; Stem Cells - cytology ; Stem Cells - drug effects</subject><ispartof>Biochemical pharmacology, 2003-07, Vol.66 (2), p.289-295</ispartof><rights>2003 Elsevier Science Inc.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-da20808d9f09e6cbd650364f0760b9ca3545ccb9f97da5c4a0b26c50eabd5eb63</citedby><cites>FETCH-LOGICAL-c457t-da20808d9f09e6cbd650364f0760b9ca3545ccb9f97da5c4a0b26c50eabd5eb63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-2952(03)00235-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14962237$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12826271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kudo, Chiho</creatorcontrib><creatorcontrib>Kori, Maya</creatorcontrib><creatorcontrib>Matsuzaki, Kiyomi</creatorcontrib><creatorcontrib>Yamai, Kenshiki</creatorcontrib><creatorcontrib>Nakajima, Atsushi</creatorcontrib><creatorcontrib>Shibuya, Atsuhito</creatorcontrib><creatorcontrib>Niwa, Hitoshi</creatorcontrib><creatorcontrib>Kamisaki, Yoshinori</creatorcontrib><creatorcontrib>Wada, Koichiro</creatorcontrib><title>Diclofenac inhibits proliferation and differentiation of neural stem cells</title><title>Biochemical pharmacology</title><addtitle>Biochem Pharmacol</addtitle><description>Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used in clinical situations as anti-inflammatory, analgesic and antipyretic drugs. However, it is still unknown whether NSAIDs have effects on the development of the central nervous system. In the present study, we investigated the effects of NSAIDs on neural stem cell (NSC) proliferation and differentiation into neurons. In contrast to aspirin, naproxen, indomethacin and ibuprofen, treatment with diclofenac (10
μM) for 2 days induced the death of NSCs in a concentration-dependent manner. Diclofenac also inhibited the proliferation of NSCs and their differentiation into neurons. Treatment with diclofenac resulted in nuclear condensation (a morphological change due to apoptosis of NSCs) 24
hr after the treatment and activated caspase-3 after 6
hr, indicating that diclofenac may cause apoptosis of neuronal cells
via activation of the caspase cascade. These results suggest that diclofenac may affect the development of the central nervous system.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Caspases - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Central nervous system</subject><subject>Diclofenac</subject><subject>Diclofenac - pharmacology</subject><subject>Enzyme Activation</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Neural stem cells</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Nonsteroidal anti-inflammatory drugs</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><issn>0006-2952</issn><issn>1873-2968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMoun78BKUXRQ_VSdKkzUnEb1nwoJ5Dmkww0k016Qr-e7vuokdPwwzPzLw8hOxTOKVA5dkTAMiSKcGOgZ8AMC5KukYmtKn5OJbNOpn8IltkO-e3RdtIukm2KGuYZDWdkIerYLveYzS2CPE1tGHIxXvqu-AxmSH0sTDRFS74scc4hOWs90XEeTJdkQecFRa7Lu-SDW-6jHurukNebq6fL-_K6ePt_eXFtLSVqIfSGQYNNE55UCht66QALisPtYRWWcNFJaxtlVe1M8JWBlomrQA0rRPYSr5DjpZ3x5gfc8yDnoW8SGAi9vOsa86VapgYQbEEbepzTuj1ewozk740Bb2QqH8k6oUhDVz_SNR03DtYPZi3M3R_WytrI3C4Aky2pvPJRBvyH1cpyRivR-58yeGo4zNg0tkGjBZdSGgH7frwT5RvrhGO9Q</recordid><startdate>20030715</startdate><enddate>20030715</enddate><creator>Kudo, Chiho</creator><creator>Kori, Maya</creator><creator>Matsuzaki, Kiyomi</creator><creator>Yamai, Kenshiki</creator><creator>Nakajima, Atsushi</creator><creator>Shibuya, Atsuhito</creator><creator>Niwa, Hitoshi</creator><creator>Kamisaki, Yoshinori</creator><creator>Wada, Koichiro</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030715</creationdate><title>Diclofenac inhibits proliferation and differentiation of neural stem cells</title><author>Kudo, Chiho ; Kori, Maya ; Matsuzaki, Kiyomi ; Yamai, Kenshiki ; Nakajima, Atsushi ; Shibuya, Atsuhito ; Niwa, Hitoshi ; Kamisaki, Yoshinori ; Wada, Koichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-da20808d9f09e6cbd650364f0760b9ca3545ccb9f97da5c4a0b26c50eabd5eb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Caspases - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Central nervous system</topic><topic>Diclofenac</topic><topic>Diclofenac - pharmacology</topic><topic>Enzyme Activation</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Neural stem cells</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Nonsteroidal anti-inflammatory drugs</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kudo, Chiho</creatorcontrib><creatorcontrib>Kori, Maya</creatorcontrib><creatorcontrib>Matsuzaki, Kiyomi</creatorcontrib><creatorcontrib>Yamai, Kenshiki</creatorcontrib><creatorcontrib>Nakajima, Atsushi</creatorcontrib><creatorcontrib>Shibuya, Atsuhito</creatorcontrib><creatorcontrib>Niwa, Hitoshi</creatorcontrib><creatorcontrib>Kamisaki, Yoshinori</creatorcontrib><creatorcontrib>Wada, Koichiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kudo, Chiho</au><au>Kori, Maya</au><au>Matsuzaki, Kiyomi</au><au>Yamai, Kenshiki</au><au>Nakajima, Atsushi</au><au>Shibuya, Atsuhito</au><au>Niwa, Hitoshi</au><au>Kamisaki, Yoshinori</au><au>Wada, Koichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diclofenac inhibits proliferation and differentiation of neural stem cells</atitle><jtitle>Biochemical pharmacology</jtitle><addtitle>Biochem Pharmacol</addtitle><date>2003-07-15</date><risdate>2003</risdate><volume>66</volume><issue>2</issue><spage>289</spage><epage>295</epage><pages>289-295</pages><issn>0006-2952</issn><eissn>1873-2968</eissn><coden>BCPCA6</coden><abstract>Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used in clinical situations as anti-inflammatory, analgesic and antipyretic drugs. However, it is still unknown whether NSAIDs have effects on the development of the central nervous system. In the present study, we investigated the effects of NSAIDs on neural stem cell (NSC) proliferation and differentiation into neurons. In contrast to aspirin, naproxen, indomethacin and ibuprofen, treatment with diclofenac (10
μM) for 2 days induced the death of NSCs in a concentration-dependent manner. Diclofenac also inhibited the proliferation of NSCs and their differentiation into neurons. Treatment with diclofenac resulted in nuclear condensation (a morphological change due to apoptosis of NSCs) 24
hr after the treatment and activated caspase-3 after 6
hr, indicating that diclofenac may cause apoptosis of neuronal cells
via activation of the caspase cascade. These results suggest that diclofenac may affect the development of the central nervous system.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>12826271</pmid><doi>10.1016/S0006-2952(03)00235-1</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical pharmacology, 2003-07, Vol.66 (2), p.289-295 |
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| subjects | Animals Anti-Inflammatory Agents, Non-Steroidal - pharmacology Apoptosis Biological and medical sciences Caspases - metabolism Cell Differentiation - drug effects Cell Division - drug effects Central nervous system Diclofenac Diclofenac - pharmacology Enzyme Activation Medical sciences Mice Neural stem cells Neurons - cytology Neurons - drug effects Nonsteroidal anti-inflammatory drugs Stem Cells - cytology Stem Cells - drug effects |
| title | Diclofenac inhibits proliferation and differentiation of neural stem cells |
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