Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma
Bovio I M, Al‐Quran S Z, Rosser C J, Algood C B, Drew P A & Allan R W (2010) Histopathology 56, 951–956 Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma Aims: To prospectively evaluate the utility of smoothelin immunohistochemic...
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| creator | Bovio, Ian M Al-Quran, Samer Z Rosser, Charles J Algood, Chester B Drew, Peter A Allan, Robert W |
| description | Bovio I M, Al‐Quran S Z, Rosser C J, Algood C B, Drew P A & Allan R W
(2010) Histopathology 56, 951–956
Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma
Aims: To prospectively evaluate the utility of smoothelin immunohistochemical expression for the evaluation of muscularis propria (MP) in diagnostic transurethral resection of bladder tumour (TURBT) specimens and cystectomies.
Methods and results: Smoothelin immunohistochemistry was performed on a total of 26 TURBT and cystectomy specimens. All but two cases (24/26) demonstrated strong (3+) or moderate (2+) immunoreactivity of the MP with smoothelin. Muscularis mucosae (MM) never displayed strong (3+) reactivity, and in only one case did the MM have moderate (2+) reactivity; in this case the MP had strong (3+) reactivity. MM intensity mirrored the intensity of reactivity of blood vessels in all cases (26/26). Using moderate or strong immunoreactivity as a cut‐off, smoothelin had a sensitivity of 92% for detecting MP and a specificity of 97% for distinguishing between MP and MM. In all unequivocal MP‐invasive and lamina proporia‐invasive cases by haematoxylin and eosin (H&E), smoothelin immunohistochemistry confirmed the original light microscopic diagnosis. In four cases in which there was equivocal MP involvement by H&E, smoothelin helped establish MP invasion.
Conclusions: Smoothelin immunohistochemistry has diagnostic utility in the evaluation of MP invasion in urothelial carcinoma. Smoothelin could be used as an adjunct to traditional H&E‐stained light microscopy and may help reduce the number of equivocal diagnoses. |
| doi_str_mv | 10.1111/j.1365-2559.2010.03575.x |
| format | Article |
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(2010) Histopathology 56, 951–956
Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma
Aims: To prospectively evaluate the utility of smoothelin immunohistochemical expression for the evaluation of muscularis propria (MP) in diagnostic transurethral resection of bladder tumour (TURBT) specimens and cystectomies.
Methods and results: Smoothelin immunohistochemistry was performed on a total of 26 TURBT and cystectomy specimens. All but two cases (24/26) demonstrated strong (3+) or moderate (2+) immunoreactivity of the MP with smoothelin. Muscularis mucosae (MM) never displayed strong (3+) reactivity, and in only one case did the MM have moderate (2+) reactivity; in this case the MP had strong (3+) reactivity. MM intensity mirrored the intensity of reactivity of blood vessels in all cases (26/26). Using moderate or strong immunoreactivity as a cut‐off, smoothelin had a sensitivity of 92% for detecting MP and a specificity of 97% for distinguishing between MP and MM. In all unequivocal MP‐invasive and lamina proporia‐invasive cases by haematoxylin and eosin (H&E), smoothelin immunohistochemistry confirmed the original light microscopic diagnosis. In four cases in which there was equivocal MP involvement by H&E, smoothelin helped establish MP invasion.
Conclusions: Smoothelin immunohistochemistry has diagnostic utility in the evaluation of MP invasion in urothelial carcinoma. Smoothelin could be used as an adjunct to traditional H&E‐stained light microscopy and may help reduce the number of equivocal diagnoses.</description><identifier>ISSN: 0309-0167</identifier><identifier>EISSN: 1365-2559</identifier><identifier>DOI: 10.1111/j.1365-2559.2010.03575.x</identifier><identifier>PMID: 20636795</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Aged ; Biological and medical sciences ; Carcinoma - metabolism ; Carcinoma - pathology ; Carcinoma - surgery ; Cystectomy ; Cytoskeletal Proteins - metabolism ; Female ; human ; Humans ; Immunohistochemistry ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Middle Aged ; Mucous Membrane - metabolism ; Mucous Membrane - pathology ; Muscle Proteins - metabolism ; muscularis propria ; Neoplasm Staging ; Nephrology. Urinary tract diseases ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; smoothelin protein ; staging ; Tumors of the urinary system ; Urinary Bladder - metabolism ; Urinary Bladder - pathology ; Urinary Bladder - surgery ; urinary bladder cancer ; Urinary Bladder Neoplasms - metabolism ; Urinary Bladder Neoplasms - pathology ; Urinary Bladder Neoplasms - surgery ; Urinary system involvement in other diseases. Miscellaneous ; Urinary tract. Prostate gland ; Urothelium - metabolism ; Urothelium - pathology</subject><ispartof>Histopathology, 2010-06, Vol.56 (7), p.951-956</ispartof><rights>2010 Blackwell Publishing Limited</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4365-1e06a1a73772658a9fda4f94c904f15713c4e299153108a55a0473207e7c99d73</citedby><cites>FETCH-LOGICAL-c4365-1e06a1a73772658a9fda4f94c904f15713c4e299153108a55a0473207e7c99d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2559.2010.03575.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2559.2010.03575.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22885771$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20636795$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bovio, Ian M</creatorcontrib><creatorcontrib>Al-Quran, Samer Z</creatorcontrib><creatorcontrib>Rosser, Charles J</creatorcontrib><creatorcontrib>Algood, Chester B</creatorcontrib><creatorcontrib>Drew, Peter A</creatorcontrib><creatorcontrib>Allan, Robert W</creatorcontrib><title>Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma</title><title>Histopathology</title><addtitle>Histopathology</addtitle><description>Bovio I M, Al‐Quran S Z, Rosser C J, Algood C B, Drew P A & Allan R W
(2010) Histopathology 56, 951–956
Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma
Aims: To prospectively evaluate the utility of smoothelin immunohistochemical expression for the evaluation of muscularis propria (MP) in diagnostic transurethral resection of bladder tumour (TURBT) specimens and cystectomies.
Methods and results: Smoothelin immunohistochemistry was performed on a total of 26 TURBT and cystectomy specimens. All but two cases (24/26) demonstrated strong (3+) or moderate (2+) immunoreactivity of the MP with smoothelin. Muscularis mucosae (MM) never displayed strong (3+) reactivity, and in only one case did the MM have moderate (2+) reactivity; in this case the MP had strong (3+) reactivity. MM intensity mirrored the intensity of reactivity of blood vessels in all cases (26/26). Using moderate or strong immunoreactivity as a cut‐off, smoothelin had a sensitivity of 92% for detecting MP and a specificity of 97% for distinguishing between MP and MM. In all unequivocal MP‐invasive and lamina proporia‐invasive cases by haematoxylin and eosin (H&E), smoothelin immunohistochemistry confirmed the original light microscopic diagnosis. In four cases in which there was equivocal MP involvement by H&E, smoothelin helped establish MP invasion.
Conclusions: Smoothelin immunohistochemistry has diagnostic utility in the evaluation of MP invasion in urothelial carcinoma. Smoothelin could be used as an adjunct to traditional H&E‐stained light microscopy and may help reduce the number of equivocal diagnoses.</description><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Carcinoma - metabolism</subject><subject>Carcinoma - pathology</subject><subject>Carcinoma - surgery</subject><subject>Cystectomy</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>Female</subject><subject>human</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mucous Membrane - metabolism</subject><subject>Mucous Membrane - pathology</subject><subject>Muscle Proteins - metabolism</subject><subject>muscularis propria</subject><subject>Neoplasm Staging</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>smoothelin protein</subject><subject>staging</subject><subject>Tumors of the urinary system</subject><subject>Urinary Bladder - metabolism</subject><subject>Urinary Bladder - pathology</subject><subject>Urinary Bladder - surgery</subject><subject>urinary bladder cancer</subject><subject>Urinary Bladder Neoplasms - metabolism</subject><subject>Urinary Bladder Neoplasms - pathology</subject><subject>Urinary Bladder Neoplasms - surgery</subject><subject>Urinary system involvement in other diseases. Miscellaneous</subject><subject>Urinary tract. Prostate gland</subject><subject>Urothelium - metabolism</subject><subject>Urothelium - pathology</subject><issn>0309-0167</issn><issn>1365-2559</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV9vFCEUxYnR2HXtVzC8GJ9mhWEYhgcfTNN_yUbTtLaP5JZhXFYGKiy6--1l3HV9LS-XwDmXe34ghClZ0LI-rheUtbyqOZeLmpRTwrjgi-0LNDtevEQzwoisCG3FCXqT0poQKlhdv0YnNWlZKySfoXg7hrBZGWc9tuOYfVjZtAl6ZcZS4w7bhAHnZIbsMPTr7PUGDyFiSMmkZP13POaks4NYlE8xPEUL2PpfkGwoLT1-dND3JmINUVsfRniLXg3gkjk91Dn6dnF-d3ZVLb9eXp99Xla6mTJQQ1qgIJgQdcs7kEMPzSAbLUkzUC4o042ppaScUdIB50CaEo8II7SUvWBz9GHft0z1M5u0USWSNs6BNyEnJRiTnZQNK8pur9QxpBTNoEqKEeJOUaIm4GqtJq5q4qom4OovcLUt1neHR_LjaPqj8R_hInh_EEDS4IYIXtv0X1d3HRclzBx92ut-W2d2zx5AXV3fTrvir_b-8m1me_RD_KHawpCrhy-X6v5uebO8v-CKsz_20KyT</recordid><startdate>201006</startdate><enddate>201006</enddate><creator>Bovio, Ian M</creator><creator>Al-Quran, Samer Z</creator><creator>Rosser, Charles J</creator><creator>Algood, Chester B</creator><creator>Drew, Peter A</creator><creator>Allan, Robert W</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201006</creationdate><title>Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma</title><author>Bovio, Ian M ; Al-Quran, Samer Z ; Rosser, Charles J ; Algood, Chester B ; Drew, Peter A ; Allan, Robert W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4365-1e06a1a73772658a9fda4f94c904f15713c4e299153108a55a0473207e7c99d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Carcinoma - metabolism</topic><topic>Carcinoma - pathology</topic><topic>Carcinoma - surgery</topic><topic>Cystectomy</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>Female</topic><topic>human</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mucous Membrane - metabolism</topic><topic>Mucous Membrane - pathology</topic><topic>Muscle Proteins - metabolism</topic><topic>muscularis propria</topic><topic>Neoplasm Staging</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>smoothelin protein</topic><topic>staging</topic><topic>Tumors of the urinary system</topic><topic>Urinary Bladder - metabolism</topic><topic>Urinary Bladder - pathology</topic><topic>Urinary Bladder - surgery</topic><topic>urinary bladder cancer</topic><topic>Urinary Bladder Neoplasms - metabolism</topic><topic>Urinary Bladder Neoplasms - pathology</topic><topic>Urinary Bladder Neoplasms - surgery</topic><topic>Urinary system involvement in other diseases. Miscellaneous</topic><topic>Urinary tract. Prostate gland</topic><topic>Urothelium - metabolism</topic><topic>Urothelium - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bovio, Ian M</creatorcontrib><creatorcontrib>Al-Quran, Samer Z</creatorcontrib><creatorcontrib>Rosser, Charles J</creatorcontrib><creatorcontrib>Algood, Chester B</creatorcontrib><creatorcontrib>Drew, Peter A</creatorcontrib><creatorcontrib>Allan, Robert W</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Histopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bovio, Ian M</au><au>Al-Quran, Samer Z</au><au>Rosser, Charles J</au><au>Algood, Chester B</au><au>Drew, Peter A</au><au>Allan, Robert W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma</atitle><jtitle>Histopathology</jtitle><addtitle>Histopathology</addtitle><date>2010-06</date><risdate>2010</risdate><volume>56</volume><issue>7</issue><spage>951</spage><epage>956</epage><pages>951-956</pages><issn>0309-0167</issn><eissn>1365-2559</eissn><abstract>Bovio I M, Al‐Quran S Z, Rosser C J, Algood C B, Drew P A & Allan R W
(2010) Histopathology 56, 951–956
Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma
Aims: To prospectively evaluate the utility of smoothelin immunohistochemical expression for the evaluation of muscularis propria (MP) in diagnostic transurethral resection of bladder tumour (TURBT) specimens and cystectomies.
Methods and results: Smoothelin immunohistochemistry was performed on a total of 26 TURBT and cystectomy specimens. All but two cases (24/26) demonstrated strong (3+) or moderate (2+) immunoreactivity of the MP with smoothelin. Muscularis mucosae (MM) never displayed strong (3+) reactivity, and in only one case did the MM have moderate (2+) reactivity; in this case the MP had strong (3+) reactivity. MM intensity mirrored the intensity of reactivity of blood vessels in all cases (26/26). Using moderate or strong immunoreactivity as a cut‐off, smoothelin had a sensitivity of 92% for detecting MP and a specificity of 97% for distinguishing between MP and MM. In all unequivocal MP‐invasive and lamina proporia‐invasive cases by haematoxylin and eosin (H&E), smoothelin immunohistochemistry confirmed the original light microscopic diagnosis. In four cases in which there was equivocal MP involvement by H&E, smoothelin helped establish MP invasion.
Conclusions: Smoothelin immunohistochemistry has diagnostic utility in the evaluation of MP invasion in urothelial carcinoma. Smoothelin could be used as an adjunct to traditional H&E‐stained light microscopy and may help reduce the number of equivocal diagnoses.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20636795</pmid><doi>10.1111/j.1365-2559.2010.03575.x</doi><tpages>6</tpages></addata></record> |
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| subjects | Aged Biological and medical sciences Carcinoma - metabolism Carcinoma - pathology Carcinoma - surgery Cystectomy Cytoskeletal Proteins - metabolism Female human Humans Immunohistochemistry Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Middle Aged Mucous Membrane - metabolism Mucous Membrane - pathology Muscle Proteins - metabolism muscularis propria Neoplasm Staging Nephrology. Urinary tract diseases Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques smoothelin protein staging Tumors of the urinary system Urinary Bladder - metabolism Urinary Bladder - pathology Urinary Bladder - surgery urinary bladder cancer Urinary Bladder Neoplasms - metabolism Urinary Bladder Neoplasms - pathology Urinary Bladder Neoplasms - surgery Urinary system involvement in other diseases. Miscellaneous Urinary tract. Prostate gland Urothelium - metabolism Urothelium - pathology |
| title | Smoothelin immunohistochemistry is a useful adjunct for assessing muscularis propria invasion in bladder carcinoma |
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