Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A, G:G:A:A, and A:A:A:A tetrads
Replacement of two to four guanines by adenines in the human telomere DNA repeat dG3(TTAG3)3 did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and...
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| Veröffentlicht in: | Biopolymers 2010-10, Vol.93 (10), p.880-886 |
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| creator | Sagi, Janos Renčiuk, Daniel Tomaško, Martin Vorlíčková, Michaela |
| description | Replacement of two to four guanines by adenines in the human telomere DNA repeat dG3(TTAG3)3 did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and PAGE in both Na+ and K+ solutions. Thermodynamic data showed that, in Na+ solution, the dG3(TTAG3)3 quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn‐guanosines. In physiological K+ solution, the highest destabilization was caused by the 4A tetrad. In K+, only the unmodified dG3(TTAG3)3 quadruplex rearranged into a K+‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010. |
| doi_str_mv | 10.1002/bip.21481 |
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Thermodynamic data showed that, in Na+ solution, the dG3(TTAG3)3 quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn‐guanosines. In physiological K+ solution, the highest destabilization was caused by the 4A tetrad. In K+, only the unmodified dG3(TTAG3)3 quadruplex rearranged into a K+‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010.</description><identifier>ISSN: 0006-3525</identifier><identifier>EISSN: 1097-0282</identifier><identifier>DOI: 10.1002/bip.21481</identifier><identifier>PMID: 20564052</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>adenine for guanine substitution ; Base Sequence ; CD spectroscopy ; Circular Dichroism ; Dimethyl Sulfoxide - metabolism ; DMS methylation ; DNA - chemistry ; DNA - genetics ; Electrophoresis, Polyacrylamide Gel ; G-Quadruplexes ; human telomere quadruplex ; Humans ; Molecular Sequence Data ; Nucleic Acid Denaturation ; Potassium ; Sodium ; Solutions ; Telomere - chemistry ; Telomere - genetics ; Temperature ; thermal stability ; thermodynamic parameters</subject><ispartof>Biopolymers, 2010-10, Vol.93 (10), p.880-886</ispartof><rights>Copyright © 2010 Wiley Periodicals, Inc.</rights><rights>Copyright (c) 2010 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3621-28f45b577a67cb3a2026299002fd0e9ca6d56b5e862598531bd4dde529268e2e3</citedby><cites>FETCH-LOGICAL-c3621-28f45b577a67cb3a2026299002fd0e9ca6d56b5e862598531bd4dde529268e2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbip.21481$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbip.21481$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20564052$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sagi, Janos</creatorcontrib><creatorcontrib>Renčiuk, Daniel</creatorcontrib><creatorcontrib>Tomaško, Martin</creatorcontrib><creatorcontrib>Vorlíčková, Michaela</creatorcontrib><title>Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A, G:G:A:A, and A:A:A:A tetrads</title><title>Biopolymers</title><addtitle>Biopolymers</addtitle><description>Replacement of two to four guanines by adenines in the human telomere DNA repeat dG3(TTAG3)3 did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and PAGE in both Na+ and K+ solutions. Thermodynamic data showed that, in Na+ solution, the dG3(TTAG3)3 quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn‐guanosines. In physiological K+ solution, the highest destabilization was caused by the 4A tetrad. In K+, only the unmodified dG3(TTAG3)3 quadruplex rearranged into a K+‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010.</description><subject>adenine for guanine substitution</subject><subject>Base Sequence</subject><subject>CD spectroscopy</subject><subject>Circular Dichroism</subject><subject>Dimethyl Sulfoxide - metabolism</subject><subject>DMS methylation</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>G-Quadruplexes</subject><subject>human telomere quadruplex</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Denaturation</subject><subject>Potassium</subject><subject>Sodium</subject><subject>Solutions</subject><subject>Telomere - chemistry</subject><subject>Telomere - genetics</subject><subject>Temperature</subject><subject>thermal stability</subject><subject>thermodynamic parameters</subject><issn>0006-3525</issn><issn>1097-0282</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1P4zAQhi0EWkp3D_yBlW8IiZSxHTtJb6VAQapg-RJHy4knbHbzUexEwL_HUOCGRta8h2ceyS8huwwmDIAf5tVqwlmcsg0yYpAlEfCUb5IRAKhISC63yY73_wDiWDD4QbY5SBWD5CNirwZj3bCq8Rk97Ur6d2hMS3usuwYd0uOLGTWtqbsHTy366qFFS_uOFl3bm6qli-lsGt5BCG8xBNNaOpu-T9D0zlj_k2yVpvb462OPyd3pye38LFpeLs7ns2VUCMVZxNMylrlMEqOSIheGA1c8y8IPSwuYFUZZqXKJqeIyS6VguY2tRckzrlLkKMZkb-1due5xQN_rpvIF1rVpsRu8ToTI0gxSFsj9NVm4znuHpV65qjHuRTPQb53q0Kl-7zSwvz-sQ96g_SI_SwzA4Rp4qmp8-d6kj87_fCqj9UXle3z-ujDuv1aJSKS-v1hotrxWNyCu9a14BQl6i20</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Sagi, Janos</creator><creator>Renčiuk, Daniel</creator><creator>Tomaško, Martin</creator><creator>Vorlíčková, Michaela</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201010</creationdate><title>Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A, G:G:A:A, and A:A:A:A tetrads</title><author>Sagi, Janos ; Renčiuk, Daniel ; Tomaško, Martin ; Vorlíčková, Michaela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3621-28f45b577a67cb3a2026299002fd0e9ca6d56b5e862598531bd4dde529268e2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>adenine for guanine substitution</topic><topic>Base Sequence</topic><topic>CD spectroscopy</topic><topic>Circular Dichroism</topic><topic>Dimethyl Sulfoxide - metabolism</topic><topic>DMS methylation</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>G-Quadruplexes</topic><topic>human telomere quadruplex</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Denaturation</topic><topic>Potassium</topic><topic>Sodium</topic><topic>Solutions</topic><topic>Telomere - chemistry</topic><topic>Telomere - genetics</topic><topic>Temperature</topic><topic>thermal stability</topic><topic>thermodynamic parameters</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sagi, Janos</creatorcontrib><creatorcontrib>Renčiuk, Daniel</creatorcontrib><creatorcontrib>Tomaško, Martin</creatorcontrib><creatorcontrib>Vorlíčková, Michaela</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biopolymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sagi, Janos</au><au>Renčiuk, Daniel</au><au>Tomaško, Martin</au><au>Vorlíčková, Michaela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A, G:G:A:A, and A:A:A:A tetrads</atitle><jtitle>Biopolymers</jtitle><addtitle>Biopolymers</addtitle><date>2010-10</date><risdate>2010</risdate><volume>93</volume><issue>10</issue><spage>880</spage><epage>886</epage><pages>880-886</pages><issn>0006-3525</issn><eissn>1097-0282</eissn><abstract>Replacement of two to four guanines by adenines in the human telomere DNA repeat dG3(TTAG3)3 did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and PAGE in both Na+ and K+ solutions. Thermodynamic data showed that, in Na+ solution, the dG3(TTAG3)3 quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn‐guanosines. In physiological K+ solution, the highest destabilization was caused by the 4A tetrad. In K+, only the unmodified dG3(TTAG3)3 quadruplex rearranged into a K+‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>20564052</pmid><doi>10.1002/bip.21481</doi><tpages>7</tpages></addata></record> |
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| subjects | adenine for guanine substitution Base Sequence CD spectroscopy Circular Dichroism Dimethyl Sulfoxide - metabolism DMS methylation DNA - chemistry DNA - genetics Electrophoresis, Polyacrylamide Gel G-Quadruplexes human telomere quadruplex Humans Molecular Sequence Data Nucleic Acid Denaturation Potassium Sodium Solutions Telomere - chemistry Telomere - genetics Temperature thermal stability thermodynamic parameters |
| title | Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A, G:G:A:A, and A:A:A:A tetrads |
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