role for the DNA-damage checkpoint kinase Chk1 in the virulence program of the fungus Ustilago maydis
During induction of the virulence program in the phytopathogenic fungus Ustilago maydis, the cell cycle is arrested on the plant surface and it is not resumed until the fungus enters the plant. The mechanism of this cell cycle arrest is unknown, but it is thought that it is necessary for the correct...
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| Veröffentlicht in: | Journal of cell science 2009-11, Vol.122 (22), p.4130-4140 |
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| container_title | Journal of cell science |
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| creator | Mielnichuk, Natalia Sgarlata, Cecilia Pérez-Martín, José |
| description | During induction of the virulence program in the phytopathogenic fungus Ustilago maydis, the cell cycle is arrested on the plant surface and it is not resumed until the fungus enters the plant. The mechanism of this cell cycle arrest is unknown, but it is thought that it is necessary for the correct implementation of the virulence program. Here, we show that this arrest takes place in the G2 phase, as a result of an increase in the inhibitory phosphorylation of the catalytic subunit of the mitotic cyclin-dependent kinase Cdk1. Sequestration in the cytoplasm of the Cdc25 phosphatase seems to be one of the reasons for the increase in inhibitory phosphorylation. Strikingly, we also report the DNA-damage checkpoint kinase Chk1 appears to be involved in this process. Our results support the emerging idea that checkpoint kinases have roles other than in the DNA-damage response, by virtue of their ability to interact with the cell cycle machinery. |
| doi_str_mv | 10.1242/jcs.052233 |
| format | Article |
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| ispartof | Journal of cell science, 2009-11, Vol.122 (22), p.4130-4140 |
| issn | 0021-9533 1477-9137 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Catalytic Domain - physiology CDC2 Protein Kinase - genetics CDC2 Protein Kinase - metabolism cdc25 Phosphatases - genetics cdc25 Phosphatases - metabolism Checkpoint Kinase 1 Cyclins - metabolism Cytoplasm - metabolism Down-Regulation - physiology Fungal Proteins - genetics Fungal Proteins - metabolism G2 Phase - physiology Genetic Engineering Phosphorylation - physiology Protein Kinases - genetics Protein Kinases - metabolism Signal Transduction - physiology Ustilago - cytology Ustilago - metabolism Ustilago - pathogenicity |
| title | role for the DNA-damage checkpoint kinase Chk1 in the virulence program of the fungus Ustilago maydis |
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