CB1 cannabinoid receptor expression is regulated by glucose and feeding in rat pancreatic islets

Endocannabinoid system is involved in food intake and energy balance. Beside the hypothalamus, pancreatic islet also expresses CB1 cannabinoid receptor, however little is known about its physiological role and regulation. Since gene expression of many specific proteins of the islet depends on the co...

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Veröffentlicht in:Regulatory peptides 2010-08, Vol.163 (1), p.81-87
Hauptverfasser: Vilches-Flores, Alonso, Delgado-Buenrostro, Norma Laura, Navarrete-Vázquez, Gabriel, Villalobos-Molina, Rafael
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container_issue 1
container_start_page 81
container_title Regulatory peptides
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creator Vilches-Flores, Alonso
Delgado-Buenrostro, Norma Laura
Navarrete-Vázquez, Gabriel
Villalobos-Molina, Rafael
description Endocannabinoid system is involved in food intake and energy balance. Beside the hypothalamus, pancreatic islet also expresses CB1 cannabinoid receptor, however little is known about its physiological role and regulation. Since gene expression of many specific proteins of the islet depends on the concentration of glucose, we studied CB1 receptor expression in response to fasting and feeding. Whole pancreas or islets were isolated from food-deprived adult Wistar rats, with or without a previous 1.5 g/kg glucose oral-intake. CB1, insulin and glucagon expressions were analyzed by confocal immunofluorescence and PCR. In vitro, rat islets were cultured at different glucose concentrations, in the presence of anandamide, or with Rimonabant analog BAR-1. CB1, insulin, glucagon, glucokinase, and PDX-1 expression were determined by real-time RT-PCR, and insulin secretion and islet content by ELISA. CB1 expression in pancreatic islets is upregulated during food restriction, and decreases in response to glucose intake or feeding. In cultured islets, 16 mmol/l glucose, BAR-1, and anandamide at low glucose reduced CB1 mRNA. Insulin, glucagon, glucokinase and PDX-1 expression increased in islets treated with anandamide at low glucose, while BAR-1 modified PDX-1 and glucagon mRNA at high glucose. Basal insulin secretion and insulin content in islets increased with anandamide, but not the glucose-stimulated response. Our results suggest that the endocannabinoid system has an important role in gene expression on islets and its close relationship with glucose response.
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Psychiatry</subject><subject>Psychopharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptor, Cannabinoid, CB1 - genetics</subject><subject>Receptor, Cannabinoid, CB1 - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rimonabant analog</subject><subject>Vertebrates: endocrinology</subject><issn>0167-0115</issn><issn>1873-1686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi1ERZfCP0DIF8QpiyeOneSCRFflQ6rUC5zNxB6vvMo6wU4Q_fe43QVunEYaPe87o4exVyC2IEC_O2wT7Weat7UoK9FsBcgnbANdKyvQnX7KNgVrKwGgLtnznA9CgGpb-Yxd1qJRoHSzYd9318AtxohDiFNwPJGleZkSp19zopzDFHnIZb1fR1zI8eGe78fVTpk4Rsc9kQtxz0PkCRc-Y7SJcAm2pEZa8gt24XHM9PI8r9i3jzdfd5-r27tPX3Yfbisre1gqVAJsrbXSXiKBwNY3qFUPWqC2A9TW-9q1Umsn684OCqRznaCmJ_S2E_KKvT31zmn6sVJezDFkS-OIkaY1m1bKvlOlsZDNibRpyjmRN3MKR0z3BoR5UGsO5qTWPKg1ojFFbYm9Ph9YhyO5v6E_Lgvw5gxgtjj6VFSE_I-r-7aHx_vvTxwVHT8DJZNtoGiLxyJ_MW4K___kN3qtmaM</recordid><startdate>20100809</startdate><enddate>20100809</enddate><creator>Vilches-Flores, Alonso</creator><creator>Delgado-Buenrostro, Norma Laura</creator><creator>Navarrete-Vázquez, Gabriel</creator><creator>Villalobos-Molina, Rafael</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100809</creationdate><title>CB1 cannabinoid receptor expression is regulated by glucose and feeding in rat pancreatic islets</title><author>Vilches-Flores, Alonso ; Delgado-Buenrostro, Norma Laura ; Navarrete-Vázquez, Gabriel ; Villalobos-Molina, Rafael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-a501c26656f3ae10a7f4a659160a6cb12cff2d7366d328cb513dd80e49eafc803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Eating</topic><topic>Endocannabinoid system</topic><topic>Fasting</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose</topic><topic>Glucose - administration &amp; dosage</topic><topic>Glucose - pharmacology</topic><topic>Islets of Langerhans - drug effects</topic><topic>Islets of Langerhans - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neuropharmacology</topic><topic>Pancreatic islets</topic><topic>Pharmacology. Drug treatments</topic><topic>Psychodysleptics: hallucinogen</topic><topic>Psychology. Psychoanalysis. 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subjects Animals
Biological and medical sciences
Eating
Endocannabinoid system
Fasting
Fundamental and applied biological sciences. Psychology
Glucose
Glucose - administration & dosage
Glucose - pharmacology
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Male
Medical sciences
Neuropharmacology
Pancreatic islets
Pharmacology. Drug treatments
Psychodysleptics: hallucinogen
Psychology. Psychoanalysis. Psychiatry
Psychopharmacology
Rats
Rats, Wistar
Receptor, Cannabinoid, CB1 - genetics
Receptor, Cannabinoid, CB1 - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Rimonabant analog
Vertebrates: endocrinology
title CB1 cannabinoid receptor expression is regulated by glucose and feeding in rat pancreatic islets
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