Angiotensin II induces EMMPRIN expression in THP-1 macrophages via the NF-kappaB pathway
Recent studies on atherosclerosis showed that an inducer of MMPs, EMMPRIN, is highly expressed in human atheromas. This suggested the important role of EMMPRIN in the stability of atherosclerotic plaques. Angiotensin II, one of the main functional peptides in the renin-angiotensin system, is involve...
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| Veröffentlicht in: | Regulatory peptides 2010-08, Vol.163 (1-3), p.88-95 |
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| creator | Yang, Li-xia Liu, Hong Guo, Rui-wei Ye, Jinshan Wang, Xian-mei Qi, Feng Guo, Chuan-ming Liang, Xing |
| description | Recent studies on atherosclerosis showed that an inducer of MMPs, EMMPRIN, is highly expressed in human atheromas. This suggested the important role of EMMPRIN in the stability of atherosclerotic plaques. Angiotensin II, one of the main functional peptides in the renin-angiotensin system, is involved in the advancement of atherosclerosis. We evaluated the effect of angiotensin II on EMMPRIN expression in THP-1 macrophages, and postulated the potential mechanisms underlying its effects.
We established THP-1 macrophages using PMA. The effect of angII on EMMPRIN expression in THP-1 macrophages was then investigated. Results from analyses of RT-PCR and western blotting showed that angII could upregulate EMMPRIN expression. This was mediated via the AT1R, but not the AT2R. The NF-kappaB inhibitor PDTC and P65 RNAi treatment could suppress the effect of angII on EMMPRIN, suggesting the involvement of the NF-kappaB pathway. A gelatin zymography assay showed that MMP-9 activity was related to EMMPRIN expression.
AngII upregulates the expression of EMMPRIN. NF-kappaB is the critical factor involved in the upregulation of EMMPRIN induced by angII. |
| doi_str_mv | 10.1016/j.regpep.2010.04.012 |
| format | Article |
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We established THP-1 macrophages using PMA. The effect of angII on EMMPRIN expression in THP-1 macrophages was then investigated. Results from analyses of RT-PCR and western blotting showed that angII could upregulate EMMPRIN expression. This was mediated via the AT1R, but not the AT2R. The NF-kappaB inhibitor PDTC and P65 RNAi treatment could suppress the effect of angII on EMMPRIN, suggesting the involvement of the NF-kappaB pathway. A gelatin zymography assay showed that MMP-9 activity was related to EMMPRIN expression.
AngII upregulates the expression of EMMPRIN. NF-kappaB is the critical factor involved in the upregulation of EMMPRIN induced by angII.</description><identifier>EISSN: 1873-1686</identifier><identifier>DOI: 10.1016/j.regpep.2010.04.012</identifier><identifier>PMID: 20451562</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Angiotensin II - pharmacology ; Basigin - genetics ; Basigin - metabolism ; Humans ; Macrophages - drug effects ; Macrophages - metabolism ; Matrix Metalloproteinase 9 - metabolism ; NF-kappa B - metabolism ; Tumor Cells, Cultured ; Up-Regulation - drug effects</subject><ispartof>Regulatory peptides, 2010-08, Vol.163 (1-3), p.88-95</ispartof><rights>Copyright (c) 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20451562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Li-xia</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Guo, Rui-wei</creatorcontrib><creatorcontrib>Ye, Jinshan</creatorcontrib><creatorcontrib>Wang, Xian-mei</creatorcontrib><creatorcontrib>Qi, Feng</creatorcontrib><creatorcontrib>Guo, Chuan-ming</creatorcontrib><creatorcontrib>Liang, Xing</creatorcontrib><title>Angiotensin II induces EMMPRIN expression in THP-1 macrophages via the NF-kappaB pathway</title><title>Regulatory peptides</title><addtitle>Regul Pept</addtitle><description>Recent studies on atherosclerosis showed that an inducer of MMPs, EMMPRIN, is highly expressed in human atheromas. This suggested the important role of EMMPRIN in the stability of atherosclerotic plaques. Angiotensin II, one of the main functional peptides in the renin-angiotensin system, is involved in the advancement of atherosclerosis. We evaluated the effect of angiotensin II on EMMPRIN expression in THP-1 macrophages, and postulated the potential mechanisms underlying its effects.
We established THP-1 macrophages using PMA. The effect of angII on EMMPRIN expression in THP-1 macrophages was then investigated. Results from analyses of RT-PCR and western blotting showed that angII could upregulate EMMPRIN expression. This was mediated via the AT1R, but not the AT2R. The NF-kappaB inhibitor PDTC and P65 RNAi treatment could suppress the effect of angII on EMMPRIN, suggesting the involvement of the NF-kappaB pathway. A gelatin zymography assay showed that MMP-9 activity was related to EMMPRIN expression.
AngII upregulates the expression of EMMPRIN. NF-kappaB is the critical factor involved in the upregulation of EMMPRIN induced by angII.</description><subject>Angiotensin II - pharmacology</subject><subject>Basigin - genetics</subject><subject>Basigin - metabolism</subject><subject>Humans</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - metabolism</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>NF-kappa B - metabolism</subject><subject>Tumor Cells, Cultured</subject><subject>Up-Regulation - drug effects</subject><issn>1873-1686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1PwkAYhDcmRhD9B8bszVPrfnS77REJSBNAYjh4a166b6FI27Xbivx7m4inSWaeTDJDyANnPmc8fD74De4sWl-w3mKBz7i4IkMeaenxMAoH5Na5A2NcaS1vyECwQHEViiH5GFe7om6xckVFk4QWlekydHS6XK7fkxXFH9ugc0Vd9RHdzNcepyVkTW33sOu57wJou0e6mnmfYC28UAvt_gTnO3Kdw9Hh_UVHZDObbiZzb_H2mkzGC88qJTwZ5DGiFkplcYBShFEOeRhGXGwFy4wMBAetM64yZrYsj40yRnENHIzRoCM5Ik9_tbapvzp0bVoWLsPjESqsO5dqKeOoX8p78vFCdtsSTWqbooTmnP5_IX8BKVFfgQ</recordid><startdate>20100809</startdate><enddate>20100809</enddate><creator>Yang, Li-xia</creator><creator>Liu, Hong</creator><creator>Guo, Rui-wei</creator><creator>Ye, Jinshan</creator><creator>Wang, Xian-mei</creator><creator>Qi, Feng</creator><creator>Guo, Chuan-ming</creator><creator>Liang, Xing</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20100809</creationdate><title>Angiotensin II induces EMMPRIN expression in THP-1 macrophages via the NF-kappaB pathway</title><author>Yang, Li-xia ; Liu, Hong ; Guo, Rui-wei ; Ye, Jinshan ; Wang, Xian-mei ; Qi, Feng ; Guo, Chuan-ming ; Liang, Xing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p552-34f9ee7255c94e3268faf66812b20cd3421a77c15c0db0f9d5dd517a1add7a783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Angiotensin II - pharmacology</topic><topic>Basigin - genetics</topic><topic>Basigin - metabolism</topic><topic>Humans</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - metabolism</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>NF-kappa B - metabolism</topic><topic>Tumor Cells, Cultured</topic><topic>Up-Regulation - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Li-xia</creatorcontrib><creatorcontrib>Liu, Hong</creatorcontrib><creatorcontrib>Guo, Rui-wei</creatorcontrib><creatorcontrib>Ye, Jinshan</creatorcontrib><creatorcontrib>Wang, Xian-mei</creatorcontrib><creatorcontrib>Qi, Feng</creatorcontrib><creatorcontrib>Guo, Chuan-ming</creatorcontrib><creatorcontrib>Liang, Xing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Regulatory peptides</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Li-xia</au><au>Liu, Hong</au><au>Guo, Rui-wei</au><au>Ye, Jinshan</au><au>Wang, Xian-mei</au><au>Qi, Feng</au><au>Guo, Chuan-ming</au><au>Liang, Xing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiotensin II induces EMMPRIN expression in THP-1 macrophages via the NF-kappaB pathway</atitle><jtitle>Regulatory peptides</jtitle><addtitle>Regul Pept</addtitle><date>2010-08-09</date><risdate>2010</risdate><volume>163</volume><issue>1-3</issue><spage>88</spage><epage>95</epage><pages>88-95</pages><eissn>1873-1686</eissn><abstract>Recent studies on atherosclerosis showed that an inducer of MMPs, EMMPRIN, is highly expressed in human atheromas. This suggested the important role of EMMPRIN in the stability of atherosclerotic plaques. Angiotensin II, one of the main functional peptides in the renin-angiotensin system, is involved in the advancement of atherosclerosis. We evaluated the effect of angiotensin II on EMMPRIN expression in THP-1 macrophages, and postulated the potential mechanisms underlying its effects.
We established THP-1 macrophages using PMA. The effect of angII on EMMPRIN expression in THP-1 macrophages was then investigated. Results from analyses of RT-PCR and western blotting showed that angII could upregulate EMMPRIN expression. This was mediated via the AT1R, but not the AT2R. The NF-kappaB inhibitor PDTC and P65 RNAi treatment could suppress the effect of angII on EMMPRIN, suggesting the involvement of the NF-kappaB pathway. A gelatin zymography assay showed that MMP-9 activity was related to EMMPRIN expression.
AngII upregulates the expression of EMMPRIN. NF-kappaB is the critical factor involved in the upregulation of EMMPRIN induced by angII.</abstract><cop>Netherlands</cop><pmid>20451562</pmid><doi>10.1016/j.regpep.2010.04.012</doi><tpages>8</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Angiotensin II - pharmacology Basigin - genetics Basigin - metabolism Humans Macrophages - drug effects Macrophages - metabolism Matrix Metalloproteinase 9 - metabolism NF-kappa B - metabolism Tumor Cells, Cultured Up-Regulation - drug effects |
| title | Angiotensin II induces EMMPRIN expression in THP-1 macrophages via the NF-kappaB pathway |
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