Effects of varicocele upon the expression of apoptosis-related proteins
Summary Varicocele‐associated apoptosis has been recognised as a cause of male infertility. Thus, we assessed the expression of somatic apoptosis‐related proteins (the typical protein‐dependent apoptosis markers) in ejaculated sperm plasma from both patients with varicocele and normal donors. We eva...
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| Veröffentlicht in: | Andrologia 2010-08, Vol.42 (4), p.225-230 |
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| creator | Chang, F.-W. Sun, G.-H. Cheng, Y.-Y. Chen, I.-C. Chien, H.-H. Wu, G.-J. |
| description | Summary
Varicocele‐associated apoptosis has been recognised as a cause of male infertility. Thus, we assessed the expression of somatic apoptosis‐related proteins (the typical protein‐dependent apoptosis markers) in ejaculated sperm plasma from both patients with varicocele and normal donors. We evaluated the relationships between certain apoptosis‐related proteins and normal semen quality. Semen samples were obtained from 25 patients with varicocele and from 10 normal fertile controls. These samples were compared using computer‐assisted semen analysis for motion parameters and manual analysis for morphology, and were also assayed for apoptosis‐related protein activation including caspase‐3, poly‐ACP‐ribose polymerase (PARP), the Bcl‐2 family (Bcl‐2, Bak) and p53 by means of immunoblot analysis. PARP, Bak and p53 were expressed substantially more in the sperm cells of the varicocele group when compared with the normal group (P |
| doi_str_mv | 10.1111/j.1439-0272.2009.00981.x |
| format | Article |
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Varicocele‐associated apoptosis has been recognised as a cause of male infertility. Thus, we assessed the expression of somatic apoptosis‐related proteins (the typical protein‐dependent apoptosis markers) in ejaculated sperm plasma from both patients with varicocele and normal donors. We evaluated the relationships between certain apoptosis‐related proteins and normal semen quality. Semen samples were obtained from 25 patients with varicocele and from 10 normal fertile controls. These samples were compared using computer‐assisted semen analysis for motion parameters and manual analysis for morphology, and were also assayed for apoptosis‐related protein activation including caspase‐3, poly‐ACP‐ribose polymerase (PARP), the Bcl‐2 family (Bcl‐2, Bak) and p53 by means of immunoblot analysis. PARP, Bak and p53 were expressed substantially more in the sperm cells of the varicocele group when compared with the normal group (P < 0.05). The expression of caspase‐3 and Bcl‐2 did not appear to differ between these two study groups. An increased expression of PARP, Bak and p53 for varicocele‐afflicted individuals indicated an increased participation by these agents in the regulating of apoptosis in the ejaculated semen from patients with varicocele, suggesting that certain protein‐development apoptotic mechanisms might originate in the cytoplasmic droplet or within mitochondria of spermatocytes and then might function within the nucleus of the cell.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1111/j.1439-0272.2009.00981.x</identifier><identifier>PMID: 20629644</identifier><identifier>CODEN: ANDRDQ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Apoptosis ; Apoptosis - physiology ; apoptosis-related proteins ; bcl-2 Homologous Antagonist-Killer Protein - biosynthesis ; Biological and medical sciences ; Caspase 3 - biosynthesis ; Ejaculation - physiology ; Gene Expression ; Gynecology. Andrology. Obstetrics ; Humans ; Infertility, Male - metabolism ; Male ; Male genital diseases ; Medical sciences ; Non tumoral diseases ; Poly(ADP-ribose) Polymerases - biosynthesis ; Proto-Oncogene Proteins c-bcl-2 - biosynthesis ; Semen Analysis ; Spermatozoa - metabolism ; Tumor Suppressor Protein p53 - biosynthesis ; varicocele ; Varicocele - physiopathology</subject><ispartof>Andrologia, 2010-08, Vol.42 (4), p.225-230</ispartof><rights>2010 Blackwell Verlag GmbH</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4361-e71ff74e6631a744466ab1775d10e0ef55c726c57fa40cd11c9de20e5da2621c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0272.2009.00981.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0272.2009.00981.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23005089$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20629644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, F.-W.</creatorcontrib><creatorcontrib>Sun, G.-H.</creatorcontrib><creatorcontrib>Cheng, Y.-Y.</creatorcontrib><creatorcontrib>Chen, I.-C.</creatorcontrib><creatorcontrib>Chien, H.-H.</creatorcontrib><creatorcontrib>Wu, G.-J.</creatorcontrib><title>Effects of varicocele upon the expression of apoptosis-related proteins</title><title>Andrologia</title><addtitle>Andrologia</addtitle><description>Summary
Varicocele‐associated apoptosis has been recognised as a cause of male infertility. Thus, we assessed the expression of somatic apoptosis‐related proteins (the typical protein‐dependent apoptosis markers) in ejaculated sperm plasma from both patients with varicocele and normal donors. We evaluated the relationships between certain apoptosis‐related proteins and normal semen quality. Semen samples were obtained from 25 patients with varicocele and from 10 normal fertile controls. These samples were compared using computer‐assisted semen analysis for motion parameters and manual analysis for morphology, and were also assayed for apoptosis‐related protein activation including caspase‐3, poly‐ACP‐ribose polymerase (PARP), the Bcl‐2 family (Bcl‐2, Bak) and p53 by means of immunoblot analysis. PARP, Bak and p53 were expressed substantially more in the sperm cells of the varicocele group when compared with the normal group (P < 0.05). The expression of caspase‐3 and Bcl‐2 did not appear to differ between these two study groups. An increased expression of PARP, Bak and p53 for varicocele‐afflicted individuals indicated an increased participation by these agents in the regulating of apoptosis in the ejaculated semen from patients with varicocele, suggesting that certain protein‐development apoptotic mechanisms might originate in the cytoplasmic droplet or within mitochondria of spermatocytes and then might function within the nucleus of the cell.</description><subject>Adult</subject><subject>Apoptosis</subject><subject>Apoptosis - physiology</subject><subject>apoptosis-related proteins</subject><subject>bcl-2 Homologous Antagonist-Killer Protein - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Caspase 3 - biosynthesis</subject><subject>Ejaculation - physiology</subject><subject>Gene Expression</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Infertility, Male - metabolism</subject><subject>Male</subject><subject>Male genital diseases</subject><subject>Medical sciences</subject><subject>Non tumoral diseases</subject><subject>Poly(ADP-ribose) Polymerases - biosynthesis</subject><subject>Proto-Oncogene Proteins c-bcl-2 - biosynthesis</subject><subject>Semen Analysis</subject><subject>Spermatozoa - metabolism</subject><subject>Tumor Suppressor Protein p53 - biosynthesis</subject><subject>varicocele</subject><subject>Varicocele - physiopathology</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1LAzEQhoMoWtS_IHsRT7tOvrvgRfyogtaLorcQsxNM3XbXzVbrvze1tQ6EJMzzTibzEpJRKGiK00lBBS9zYJoVDKAs0hrSYrFFBpvENhkAB54Lqco9chjjBFIIqbUQu2SPgWKlEmJARlfeo-tj1vjs03bBNQ5rzOZtM8v6N8xw0XYYY0jXRNi2afsmhph3WNseq6ztmh7DLB6QHW_riIfrfZ88XV89Xtzkdw-j24vzu9wJrmiOmnqvBSrFqU29CKXsK9VaVhQQ0EvpNFNOam8FuIpSV1bIAGVlmWLU8X1ysqqbHv6YY-zNNMTUcm1n2Myj0ZyXQ05BJfJoTc5fp1iZtgtT232bv78n4HgN2Ohs7Ts7cyH-cxxAwrBM3NmK-wo1fm_yFMzSDjMxy6mb5dTN0g7za4dZmPPxZTokeb6Sh9jjYiO33btRmmtpnscjw59Bj9k9Ny_8Byt_i1U</recordid><startdate>201008</startdate><enddate>201008</enddate><creator>Chang, F.-W.</creator><creator>Sun, G.-H.</creator><creator>Cheng, Y.-Y.</creator><creator>Chen, I.-C.</creator><creator>Chien, H.-H.</creator><creator>Wu, G.-J.</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201008</creationdate><title>Effects of varicocele upon the expression of apoptosis-related proteins</title><author>Chang, F.-W. ; Sun, G.-H. ; Cheng, Y.-Y. ; Chen, I.-C. ; Chien, H.-H. ; Wu, G.-J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4361-e71ff74e6631a744466ab1775d10e0ef55c726c57fa40cd11c9de20e5da2621c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Apoptosis</topic><topic>Apoptosis - physiology</topic><topic>apoptosis-related proteins</topic><topic>bcl-2 Homologous Antagonist-Killer Protein - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Caspase 3 - biosynthesis</topic><topic>Ejaculation - physiology</topic><topic>Gene Expression</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Infertility, Male - metabolism</topic><topic>Male</topic><topic>Male genital diseases</topic><topic>Medical sciences</topic><topic>Non tumoral diseases</topic><topic>Poly(ADP-ribose) Polymerases - biosynthesis</topic><topic>Proto-Oncogene Proteins c-bcl-2 - biosynthesis</topic><topic>Semen Analysis</topic><topic>Spermatozoa - metabolism</topic><topic>Tumor Suppressor Protein p53 - biosynthesis</topic><topic>varicocele</topic><topic>Varicocele - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, F.-W.</creatorcontrib><creatorcontrib>Sun, G.-H.</creatorcontrib><creatorcontrib>Cheng, Y.-Y.</creatorcontrib><creatorcontrib>Chen, I.-C.</creatorcontrib><creatorcontrib>Chien, H.-H.</creatorcontrib><creatorcontrib>Wu, G.-J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, F.-W.</au><au>Sun, G.-H.</au><au>Cheng, Y.-Y.</au><au>Chen, I.-C.</au><au>Chien, H.-H.</au><au>Wu, G.-J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of varicocele upon the expression of apoptosis-related proteins</atitle><jtitle>Andrologia</jtitle><addtitle>Andrologia</addtitle><date>2010-08</date><risdate>2010</risdate><volume>42</volume><issue>4</issue><spage>225</spage><epage>230</epage><pages>225-230</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><coden>ANDRDQ</coden><abstract>Summary
Varicocele‐associated apoptosis has been recognised as a cause of male infertility. Thus, we assessed the expression of somatic apoptosis‐related proteins (the typical protein‐dependent apoptosis markers) in ejaculated sperm plasma from both patients with varicocele and normal donors. We evaluated the relationships between certain apoptosis‐related proteins and normal semen quality. Semen samples were obtained from 25 patients with varicocele and from 10 normal fertile controls. These samples were compared using computer‐assisted semen analysis for motion parameters and manual analysis for morphology, and were also assayed for apoptosis‐related protein activation including caspase‐3, poly‐ACP‐ribose polymerase (PARP), the Bcl‐2 family (Bcl‐2, Bak) and p53 by means of immunoblot analysis. PARP, Bak and p53 were expressed substantially more in the sperm cells of the varicocele group when compared with the normal group (P < 0.05). The expression of caspase‐3 and Bcl‐2 did not appear to differ between these two study groups. An increased expression of PARP, Bak and p53 for varicocele‐afflicted individuals indicated an increased participation by these agents in the regulating of apoptosis in the ejaculated semen from patients with varicocele, suggesting that certain protein‐development apoptotic mechanisms might originate in the cytoplasmic droplet or within mitochondria of spermatocytes and then might function within the nucleus of the cell.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20629644</pmid><doi>10.1111/j.1439-0272.2009.00981.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Apoptosis Apoptosis - physiology apoptosis-related proteins bcl-2 Homologous Antagonist-Killer Protein - biosynthesis Biological and medical sciences Caspase 3 - biosynthesis Ejaculation - physiology Gene Expression Gynecology. Andrology. Obstetrics Humans Infertility, Male - metabolism Male Male genital diseases Medical sciences Non tumoral diseases Poly(ADP-ribose) Polymerases - biosynthesis Proto-Oncogene Proteins c-bcl-2 - biosynthesis Semen Analysis Spermatozoa - metabolism Tumor Suppressor Protein p53 - biosynthesis varicocele Varicocele - physiopathology |
| title | Effects of varicocele upon the expression of apoptosis-related proteins |
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