Porcine oocytes denuded before maturation can develop to the blastocyst stage if provided a cumulous cell-derived coculture system
The physiological role of cumulus cells (CC) surrounding oocytes is particularly important for normal cytoplasmic maturation of oocytes. However, removal of CC from oocytes is inevitable for some embryo manipulation techniques, such as germinal vesicle (GV) transfer, somatic cell haploidization, and...
Gespeichert in:
| Veröffentlicht in: | Journal of animal science 2010-08, Vol.88 (8), p.2604-2610 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2610 |
|---|---|
| container_issue | 8 |
| container_start_page | 2604 |
| container_title | Journal of animal science |
| container_volume | 88 |
| creator | Zhang, X Miao, Y Zhao, J.-G Spate, L Bennett, M.W Murphy, C.N Schatten, H Prather, R.S |
| description | The physiological role of cumulus cells (CC) surrounding oocytes is particularly important for normal cytoplasmic maturation of oocytes. However, removal of CC from oocytes is inevitable for some embryo manipulation techniques, such as germinal vesicle (GV) transfer, somatic cell haploidization, and oocyte cryopreservation. The present study was designed to determine an optimal method to culture porcine denuded oocytes (DO). The results indicated CC from cumulus-oocyte complexes at the GV stage (GVCC) or at the metaphase II stage, and mural granulosa cells could not improve the maturation of DO. However, GVCC could enhance the development of matured porcine DO after fertilization; the percentage of blastocysts was increased from 1.1 to 17.2% (P < 0.05), and the relative value of the x-axis and y-axis of spindles was also increased (P < 0.05). Coculture with GVCC had no effect on the distribution of mitochondria and cortical granules. The results contribute to our understanding of the mechanisms by which CC promote oocyte maturation and contribute to optimization of protocols for in vitro maturation of DO. |
| doi_str_mv | 10.2527/jas.2009-2714 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_733974866</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733974866</sourcerecordid><originalsourceid>FETCH-LOGICAL-f264t-c314e51b72f61ee7f6c6e348dfdb21e30777f37b31ddbed8b808806be987a2e23</originalsourceid><addsrcrecordid>eNpFkElLBDEQhYMoOi5Hr5qLeGrN0p1kjjK4gaCgnpssFW3p7oxJesCrv9wMjngqqPreq8dD6JiSC9Ywefmh0wUjZF4xSestNKMNaypOBd9GM0IYrZSibA_tp_RBCGXNvNlFe4xwxZQUM_T9FKLtRsAh2K8MCTsYJwcOG_AhAh50nqLOXRix1WO5rqAPS5wDzu-ATa9TLsKUccr6DXDn8TKGVbd20NhOw9SHKWELfV85iN2q7G2wU19cAacihOEQ7XjdJzjazAP0enP9srirHh5v7xdXD5Vnos6V5bSGhhrJvKAA0gsrgNfKeWcYBU6klJ5Lw6lzBpwyiihFhIG5kpoB4wfo_Ne3JPycIOV26NI6mR6hhGwl53NZKyEKebIhJzOAa5exG3T8av9qK8DZBtDJ6t5HPdou_XOcNETV65env5zXodVvsTCvz4xQTqgSkijKfwAvkIgf</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733974866</pqid></control><display><type>article</type><title>Porcine oocytes denuded before maturation can develop to the blastocyst stage if provided a cumulous cell-derived coculture system</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><creator>Zhang, X ; Miao, Y ; Zhao, J.-G ; Spate, L ; Bennett, M.W ; Murphy, C.N ; Schatten, H ; Prather, R.S</creator><creatorcontrib>Zhang, X ; Miao, Y ; Zhao, J.-G ; Spate, L ; Bennett, M.W ; Murphy, C.N ; Schatten, H ; Prather, R.S</creatorcontrib><description>The physiological role of cumulus cells (CC) surrounding oocytes is particularly important for normal cytoplasmic maturation of oocytes. However, removal of CC from oocytes is inevitable for some embryo manipulation techniques, such as germinal vesicle (GV) transfer, somatic cell haploidization, and oocyte cryopreservation. The present study was designed to determine an optimal method to culture porcine denuded oocytes (DO). The results indicated CC from cumulus-oocyte complexes at the GV stage (GVCC) or at the metaphase II stage, and mural granulosa cells could not improve the maturation of DO. However, GVCC could enhance the development of matured porcine DO after fertilization; the percentage of blastocysts was increased from 1.1 to 17.2% (P < 0.05), and the relative value of the x-axis and y-axis of spindles was also increased (P < 0.05). Coculture with GVCC had no effect on the distribution of mitochondria and cortical granules. The results contribute to our understanding of the mechanisms by which CC promote oocyte maturation and contribute to optimization of protocols for in vitro maturation of DO.</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>DOI: 10.2527/jas.2009-2714</identifier><identifier>PMID: 20382876</identifier><language>eng</language><publisher>Champaign, IL: American Society of Animal Science</publisher><subject>Animal productions ; Animals ; Biological and medical sciences ; blastocyst ; Blastocyst - physiology ; cell cycle ; coculture ; Coculture Techniques - methods ; cultured cells ; cumulus cells ; Cumulus Cells - physiology ; cumulus oophorus ; cytoplasm ; embryogenesis ; Female ; fertilization (reproduction) ; Fertilization in Vitro - veterinary ; food animals ; Fundamental and applied biological sciences. Psychology ; granulosa cells ; Granulosa Cells - physiology ; in vitro studies ; laboratory techniques ; Metaphase - physiology ; Microscopy, Fluorescence - veterinary ; mitochondria ; Mitochondria - physiology ; oocyte maturation ; oocytes ; Oocytes - growth & development ; Oocytes - physiology ; Oocytes - ultrastructure ; optimization ; Spindle Apparatus - physiology ; swine ; Terrestrial animal productions ; Vertebrates</subject><ispartof>Journal of animal science, 2010-08, Vol.88 (8), p.2604-2610</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23050842$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20382876$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, X</creatorcontrib><creatorcontrib>Miao, Y</creatorcontrib><creatorcontrib>Zhao, J.-G</creatorcontrib><creatorcontrib>Spate, L</creatorcontrib><creatorcontrib>Bennett, M.W</creatorcontrib><creatorcontrib>Murphy, C.N</creatorcontrib><creatorcontrib>Schatten, H</creatorcontrib><creatorcontrib>Prather, R.S</creatorcontrib><title>Porcine oocytes denuded before maturation can develop to the blastocyst stage if provided a cumulous cell-derived coculture system</title><title>Journal of animal science</title><addtitle>J Anim Sci</addtitle><description>The physiological role of cumulus cells (CC) surrounding oocytes is particularly important for normal cytoplasmic maturation of oocytes. However, removal of CC from oocytes is inevitable for some embryo manipulation techniques, such as germinal vesicle (GV) transfer, somatic cell haploidization, and oocyte cryopreservation. The present study was designed to determine an optimal method to culture porcine denuded oocytes (DO). The results indicated CC from cumulus-oocyte complexes at the GV stage (GVCC) or at the metaphase II stage, and mural granulosa cells could not improve the maturation of DO. However, GVCC could enhance the development of matured porcine DO after fertilization; the percentage of blastocysts was increased from 1.1 to 17.2% (P < 0.05), and the relative value of the x-axis and y-axis of spindles was also increased (P < 0.05). Coculture with GVCC had no effect on the distribution of mitochondria and cortical granules. The results contribute to our understanding of the mechanisms by which CC promote oocyte maturation and contribute to optimization of protocols for in vitro maturation of DO.</description><subject>Animal productions</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>blastocyst</subject><subject>Blastocyst - physiology</subject><subject>cell cycle</subject><subject>coculture</subject><subject>Coculture Techniques - methods</subject><subject>cultured cells</subject><subject>cumulus cells</subject><subject>Cumulus Cells - physiology</subject><subject>cumulus oophorus</subject><subject>cytoplasm</subject><subject>embryogenesis</subject><subject>Female</subject><subject>fertilization (reproduction)</subject><subject>Fertilization in Vitro - veterinary</subject><subject>food animals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>granulosa cells</subject><subject>Granulosa Cells - physiology</subject><subject>in vitro studies</subject><subject>laboratory techniques</subject><subject>Metaphase - physiology</subject><subject>Microscopy, Fluorescence - veterinary</subject><subject>mitochondria</subject><subject>Mitochondria - physiology</subject><subject>oocyte maturation</subject><subject>oocytes</subject><subject>Oocytes - growth & development</subject><subject>Oocytes - physiology</subject><subject>Oocytes - ultrastructure</subject><subject>optimization</subject><subject>Spindle Apparatus - physiology</subject><subject>swine</subject><subject>Terrestrial animal productions</subject><subject>Vertebrates</subject><issn>0021-8812</issn><issn>1525-3163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkElLBDEQhYMoOi5Hr5qLeGrN0p1kjjK4gaCgnpssFW3p7oxJesCrv9wMjngqqPreq8dD6JiSC9Ywefmh0wUjZF4xSestNKMNaypOBd9GM0IYrZSibA_tp_RBCGXNvNlFe4xwxZQUM_T9FKLtRsAh2K8MCTsYJwcOG_AhAh50nqLOXRix1WO5rqAPS5wDzu-ATa9TLsKUccr6DXDn8TKGVbd20NhOw9SHKWELfV85iN2q7G2wU19cAacihOEQ7XjdJzjazAP0enP9srirHh5v7xdXD5Vnos6V5bSGhhrJvKAA0gsrgNfKeWcYBU6klJ5Lw6lzBpwyiihFhIG5kpoB4wfo_Ne3JPycIOV26NI6mR6hhGwl53NZKyEKebIhJzOAa5exG3T8av9qK8DZBtDJ6t5HPdou_XOcNETV65env5zXodVvsTCvz4xQTqgSkijKfwAvkIgf</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Zhang, X</creator><creator>Miao, Y</creator><creator>Zhao, J.-G</creator><creator>Spate, L</creator><creator>Bennett, M.W</creator><creator>Murphy, C.N</creator><creator>Schatten, H</creator><creator>Prather, R.S</creator><general>American Society of Animal Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20100801</creationdate><title>Porcine oocytes denuded before maturation can develop to the blastocyst stage if provided a cumulous cell-derived coculture system</title><author>Zhang, X ; Miao, Y ; Zhao, J.-G ; Spate, L ; Bennett, M.W ; Murphy, C.N ; Schatten, H ; Prather, R.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f264t-c314e51b72f61ee7f6c6e348dfdb21e30777f37b31ddbed8b808806be987a2e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animal productions</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>blastocyst</topic><topic>Blastocyst - physiology</topic><topic>cell cycle</topic><topic>coculture</topic><topic>Coculture Techniques - methods</topic><topic>cultured cells</topic><topic>cumulus cells</topic><topic>Cumulus Cells - physiology</topic><topic>cumulus oophorus</topic><topic>cytoplasm</topic><topic>embryogenesis</topic><topic>Female</topic><topic>fertilization (reproduction)</topic><topic>Fertilization in Vitro - veterinary</topic><topic>food animals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>granulosa cells</topic><topic>Granulosa Cells - physiology</topic><topic>in vitro studies</topic><topic>laboratory techniques</topic><topic>Metaphase - physiology</topic><topic>Microscopy, Fluorescence - veterinary</topic><topic>mitochondria</topic><topic>Mitochondria - physiology</topic><topic>oocyte maturation</topic><topic>oocytes</topic><topic>Oocytes - growth & development</topic><topic>Oocytes - physiology</topic><topic>Oocytes - ultrastructure</topic><topic>optimization</topic><topic>Spindle Apparatus - physiology</topic><topic>swine</topic><topic>Terrestrial animal productions</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, X</creatorcontrib><creatorcontrib>Miao, Y</creatorcontrib><creatorcontrib>Zhao, J.-G</creatorcontrib><creatorcontrib>Spate, L</creatorcontrib><creatorcontrib>Bennett, M.W</creatorcontrib><creatorcontrib>Murphy, C.N</creatorcontrib><creatorcontrib>Schatten, H</creatorcontrib><creatorcontrib>Prather, R.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, X</au><au>Miao, Y</au><au>Zhao, J.-G</au><au>Spate, L</au><au>Bennett, M.W</au><au>Murphy, C.N</au><au>Schatten, H</au><au>Prather, R.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porcine oocytes denuded before maturation can develop to the blastocyst stage if provided a cumulous cell-derived coculture system</atitle><jtitle>Journal of animal science</jtitle><addtitle>J Anim Sci</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>88</volume><issue>8</issue><spage>2604</spage><epage>2610</epage><pages>2604-2610</pages><issn>0021-8812</issn><eissn>1525-3163</eissn><abstract>The physiological role of cumulus cells (CC) surrounding oocytes is particularly important for normal cytoplasmic maturation of oocytes. However, removal of CC from oocytes is inevitable for some embryo manipulation techniques, such as germinal vesicle (GV) transfer, somatic cell haploidization, and oocyte cryopreservation. The present study was designed to determine an optimal method to culture porcine denuded oocytes (DO). The results indicated CC from cumulus-oocyte complexes at the GV stage (GVCC) or at the metaphase II stage, and mural granulosa cells could not improve the maturation of DO. However, GVCC could enhance the development of matured porcine DO after fertilization; the percentage of blastocysts was increased from 1.1 to 17.2% (P < 0.05), and the relative value of the x-axis and y-axis of spindles was also increased (P < 0.05). Coculture with GVCC had no effect on the distribution of mitochondria and cortical granules. The results contribute to our understanding of the mechanisms by which CC promote oocyte maturation and contribute to optimization of protocols for in vitro maturation of DO.</abstract><cop>Champaign, IL</cop><pub>American Society of Animal Science</pub><pmid>20382876</pmid><doi>10.2527/jas.2009-2714</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8812 |
| ispartof | Journal of animal science, 2010-08, Vol.88 (8), p.2604-2610 |
| issn | 0021-8812 1525-3163 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733974866 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Animal productions Animals Biological and medical sciences blastocyst Blastocyst - physiology cell cycle coculture Coculture Techniques - methods cultured cells cumulus cells Cumulus Cells - physiology cumulus oophorus cytoplasm embryogenesis Female fertilization (reproduction) Fertilization in Vitro - veterinary food animals Fundamental and applied biological sciences. Psychology granulosa cells Granulosa Cells - physiology in vitro studies laboratory techniques Metaphase - physiology Microscopy, Fluorescence - veterinary mitochondria Mitochondria - physiology oocyte maturation oocytes Oocytes - growth & development Oocytes - physiology Oocytes - ultrastructure optimization Spindle Apparatus - physiology swine Terrestrial animal productions Vertebrates |
| title | Porcine oocytes denuded before maturation can develop to the blastocyst stage if provided a cumulous cell-derived coculture system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T13%3A14%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Porcine%20oocytes%20denuded%20before%20maturation%20can%20develop%20to%20the%20blastocyst%20stage%20if%20provided%20a%20cumulous%20cell-derived%20coculture%20system&rft.jtitle=Journal%20of%20animal%20science&rft.au=Zhang,%20X&rft.date=2010-08-01&rft.volume=88&rft.issue=8&rft.spage=2604&rft.epage=2610&rft.pages=2604-2610&rft.issn=0021-8812&rft.eissn=1525-3163&rft_id=info:doi/10.2527/jas.2009-2714&rft_dat=%3Cproquest_pubme%3E733974866%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733974866&rft_id=info:pmid/20382876&rfr_iscdi=true |