Serological analysis of Helicobacter hepaticus infection in patients with biliary and pancreatic diseases
Background and Aims: Infection with Helicobacter hepaticus has been associated with development of hepatocellular carcinoma and gallstones in animal models. In humans, however, the association of H. hepaticus infection with biliary and pancreatic diseases has not been elucidated. The aim of this st...
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| Veröffentlicht in: | Journal of gastroenterology and hepatology 2010-05, Vol.25 (s1), p.S86-S89 |
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| container_title | Journal of gastroenterology and hepatology |
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| creator | Shimoyama, Tadashi Takahashi, Ryoki Abe, Daijiro Mizuki, Ichiro Endo, Tetsu Fukuda, Shinsaku |
| description | Background and Aims: Infection with Helicobacter hepaticus has been associated with development of hepatocellular carcinoma and gallstones in animal models. In humans, however, the association of H. hepaticus infection with biliary and pancreatic diseases has not been elucidated. The aim of this study was to serologically examine the prevalence of H. hepaticus infection in patients with biliary and pancreatic diseases.
Methods: Serum samples obtained from 55 patients with cholelithiasis, 18 with bile duct or gallbladder cancer and 19 with pancreatic cancer were studied. Sera were obtained from 34 control subjects who underwent endoscopy and were diagnosed as not having peptic ulcers or cancers. Seropositivity of H. hepaticus was examined by western blot analysis using a H. hepaticus‐specific antigen. To validate the specificity, positive sera were also tested after absorption with H. hepaticus whole‐cell sonicate. Serum samples were also tested for the presence of anti‐Helicobacter pylori antibody.
Results: Prevalence of antibody to H. hepaticus‐specific antigen in patients with bile tract cancer was 38.8% and was significantly higher than in control subjects (13.1%, P |
| doi_str_mv | 10.1111/j.1440-1746.2010.06224.x |
| format | Article |
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Methods: Serum samples obtained from 55 patients with cholelithiasis, 18 with bile duct or gallbladder cancer and 19 with pancreatic cancer were studied. Sera were obtained from 34 control subjects who underwent endoscopy and were diagnosed as not having peptic ulcers or cancers. Seropositivity of H. hepaticus was examined by western blot analysis using a H. hepaticus‐specific antigen. To validate the specificity, positive sera were also tested after absorption with H. hepaticus whole‐cell sonicate. Serum samples were also tested for the presence of anti‐Helicobacter pylori antibody.
Results: Prevalence of antibody to H. hepaticus‐specific antigen in patients with bile tract cancer was 38.8% and was significantly higher than in control subjects (13.1%, P < 0.05). Prevalence of antibody to H. hepaticus‐specific antigen was 18.2% and 10.5% in patients with cholelithiasis and pancreatic cancer, respectively. Seropositivity for H. pylori was similar in all groups. Detection of the H. hepaticus‐specific band was significantly decreased after the sera were absorbed with H. hepaticus whole‐cell sonicate.
Conclusion: Infection with H. hepaticus might be associated with bile duct cancer. Results obtained from absorbed sera suggested high specificity of the western blot analysis.</description><identifier>ISSN: 0815-9319</identifier><identifier>EISSN: 1440-1746</identifier><identifier>DOI: 10.1111/j.1440-1746.2010.06224.x</identifier><identifier>PMID: 20586873</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Aged ; Aged, 80 and over ; Antibodies, Bacterial - blood ; Bile Duct Neoplasms - blood ; Bile Duct Neoplasms - epidemiology ; biliary diseases ; Biliary Tract Diseases - blood ; Biliary Tract Diseases - epidemiology ; Biological and medical sciences ; Biomarkers - blood ; Blotting, Western ; Case-Control Studies ; Chi-Square Distribution ; Cholelithiasis - blood ; Cholelithiasis - epidemiology ; Female ; Gallbladder Neoplasms - blood ; Gallbladder Neoplasms - epidemiology ; Gastroenterology. Liver. Pancreas. Abdomen ; Helicobacter hepaticus ; Helicobacter hepaticus - immunology ; Helicobacter Infections - blood ; Helicobacter Infections - epidemiology ; Helicobacter Infections - microbiology ; Humans ; Japan - epidemiology ; Male ; Medical sciences ; Middle Aged ; Pancreatic Neoplasms - blood ; Pancreatic Neoplasms - epidemiology ; serodiagnosis ; Seroepidemiologic Studies</subject><ispartof>Journal of gastroenterology and hepatology, 2010-05, Vol.25 (s1), p.S86-S89</ispartof><rights>Journal compilation © 2010 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4364-58c47867657379a489c0a166edffbc6dcc543cf17b5aaaf4e7e5ca1d08754cdb3</citedby><cites>FETCH-LOGICAL-c4364-58c47867657379a489c0a166edffbc6dcc543cf17b5aaaf4e7e5ca1d08754cdb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1440-1746.2010.06224.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1440-1746.2010.06224.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,1411,23909,23910,25118,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22806257$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20586873$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimoyama, Tadashi</creatorcontrib><creatorcontrib>Takahashi, Ryoki</creatorcontrib><creatorcontrib>Abe, Daijiro</creatorcontrib><creatorcontrib>Mizuki, Ichiro</creatorcontrib><creatorcontrib>Endo, Tetsu</creatorcontrib><creatorcontrib>Fukuda, Shinsaku</creatorcontrib><title>Serological analysis of Helicobacter hepaticus infection in patients with biliary and pancreatic diseases</title><title>Journal of gastroenterology and hepatology</title><addtitle>J Gastroenterol Hepatol</addtitle><description>Background and Aims: Infection with Helicobacter hepaticus has been associated with development of hepatocellular carcinoma and gallstones in animal models. In humans, however, the association of H. hepaticus infection with biliary and pancreatic diseases has not been elucidated. The aim of this study was to serologically examine the prevalence of H. hepaticus infection in patients with biliary and pancreatic diseases.
Methods: Serum samples obtained from 55 patients with cholelithiasis, 18 with bile duct or gallbladder cancer and 19 with pancreatic cancer were studied. Sera were obtained from 34 control subjects who underwent endoscopy and were diagnosed as not having peptic ulcers or cancers. Seropositivity of H. hepaticus was examined by western blot analysis using a H. hepaticus‐specific antigen. To validate the specificity, positive sera were also tested after absorption with H. hepaticus whole‐cell sonicate. Serum samples were also tested for the presence of anti‐Helicobacter pylori antibody.
Results: Prevalence of antibody to H. hepaticus‐specific antigen in patients with bile tract cancer was 38.8% and was significantly higher than in control subjects (13.1%, P < 0.05). Prevalence of antibody to H. hepaticus‐specific antigen was 18.2% and 10.5% in patients with cholelithiasis and pancreatic cancer, respectively. Seropositivity for H. pylori was similar in all groups. Detection of the H. hepaticus‐specific band was significantly decreased after the sera were absorbed with H. hepaticus whole‐cell sonicate.
Conclusion: Infection with H. hepaticus might be associated with bile duct cancer. Results obtained from absorbed sera suggested high specificity of the western blot analysis.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antibodies, Bacterial - blood</subject><subject>Bile Duct Neoplasms - blood</subject><subject>Bile Duct Neoplasms - epidemiology</subject><subject>biliary diseases</subject><subject>Biliary Tract Diseases - blood</subject><subject>Biliary Tract Diseases - epidemiology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Blotting, Western</subject><subject>Case-Control Studies</subject><subject>Chi-Square Distribution</subject><subject>Cholelithiasis - blood</subject><subject>Cholelithiasis - epidemiology</subject><subject>Female</subject><subject>Gallbladder Neoplasms - blood</subject><subject>Gallbladder Neoplasms - epidemiology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Helicobacter hepaticus</subject><subject>Helicobacter hepaticus - immunology</subject><subject>Helicobacter Infections - blood</subject><subject>Helicobacter Infections - epidemiology</subject><subject>Helicobacter Infections - microbiology</subject><subject>Humans</subject><subject>Japan - epidemiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pancreatic Neoplasms - blood</subject><subject>Pancreatic Neoplasms - epidemiology</subject><subject>serodiagnosis</subject><subject>Seroepidemiologic Studies</subject><issn>0815-9319</issn><issn>1440-1746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE-P0zAQxS0EYkvhKyBfEKcUO_6bAwdUQQsscFhYjpbjjFkXNyl2qm2_PQ4t5YovHs289-z5IYQpWdByXm0WlHNSUcXloialS2Rd88XhAZpdBg_RjGgqqobR5go9yXlDCOFEicfoqiZCS63YDIUbSEMcfgRnI7a9jcccMh48XkMMbmitGyHhO9jZMbh9xqH34MYw9KXCUxP6MeP7MN7hNsRg07GkdGXSuwSTB3chg82Qn6JH3sYMz873HH179_brcl1df1m9X765rhxnkldCO660VFIophrLdeOIpVJC533rZOec4Mx5qlphrfUcFAhnaUe0Etx1LZujl6fcXRp-7SGPZhuygxhtD8M-G8VYowoTUpT6pHRpyDmBN7sUtmUFQ4mZOJuNmXCaCaeZOJs_nM2hWJ-fH9m3W-guxr9gi-DFWWBzQetTARLyP12tS1RZcY5en3T3IcLxvz9gPqzWU1X81ckf8giHi9-mn0YWgMJ8_7wykt7e3shPH82S_QaCEaoG</recordid><startdate>201005</startdate><enddate>201005</enddate><creator>Shimoyama, Tadashi</creator><creator>Takahashi, Ryoki</creator><creator>Abe, Daijiro</creator><creator>Mizuki, Ichiro</creator><creator>Endo, Tetsu</creator><creator>Fukuda, Shinsaku</creator><general>Blackwell Publishing Asia</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201005</creationdate><title>Serological analysis of Helicobacter hepaticus infection in patients with biliary and pancreatic diseases</title><author>Shimoyama, Tadashi ; Takahashi, Ryoki ; Abe, Daijiro ; Mizuki, Ichiro ; Endo, Tetsu ; Fukuda, Shinsaku</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4364-58c47867657379a489c0a166edffbc6dcc543cf17b5aaaf4e7e5ca1d08754cdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antibodies, Bacterial - blood</topic><topic>Bile Duct Neoplasms - blood</topic><topic>Bile Duct Neoplasms - epidemiology</topic><topic>biliary diseases</topic><topic>Biliary Tract Diseases - blood</topic><topic>Biliary Tract Diseases - epidemiology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - blood</topic><topic>Blotting, Western</topic><topic>Case-Control Studies</topic><topic>Chi-Square Distribution</topic><topic>Cholelithiasis - blood</topic><topic>Cholelithiasis - epidemiology</topic><topic>Female</topic><topic>Gallbladder Neoplasms - blood</topic><topic>Gallbladder Neoplasms - epidemiology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Helicobacter hepaticus</topic><topic>Helicobacter hepaticus - immunology</topic><topic>Helicobacter Infections - blood</topic><topic>Helicobacter Infections - epidemiology</topic><topic>Helicobacter Infections - microbiology</topic><topic>Humans</topic><topic>Japan - epidemiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pancreatic Neoplasms - blood</topic><topic>Pancreatic Neoplasms - epidemiology</topic><topic>serodiagnosis</topic><topic>Seroepidemiologic Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimoyama, Tadashi</creatorcontrib><creatorcontrib>Takahashi, Ryoki</creatorcontrib><creatorcontrib>Abe, Daijiro</creatorcontrib><creatorcontrib>Mizuki, Ichiro</creatorcontrib><creatorcontrib>Endo, Tetsu</creatorcontrib><creatorcontrib>Fukuda, Shinsaku</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of gastroenterology and hepatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimoyama, Tadashi</au><au>Takahashi, Ryoki</au><au>Abe, Daijiro</au><au>Mizuki, Ichiro</au><au>Endo, Tetsu</au><au>Fukuda, Shinsaku</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serological analysis of Helicobacter hepaticus infection in patients with biliary and pancreatic diseases</atitle><jtitle>Journal of gastroenterology and hepatology</jtitle><addtitle>J Gastroenterol Hepatol</addtitle><date>2010-05</date><risdate>2010</risdate><volume>25</volume><issue>s1</issue><spage>S86</spage><epage>S89</epage><pages>S86-S89</pages><issn>0815-9319</issn><eissn>1440-1746</eissn><abstract>Background and Aims: Infection with Helicobacter hepaticus has been associated with development of hepatocellular carcinoma and gallstones in animal models. In humans, however, the association of H. hepaticus infection with biliary and pancreatic diseases has not been elucidated. The aim of this study was to serologically examine the prevalence of H. hepaticus infection in patients with biliary and pancreatic diseases.
Methods: Serum samples obtained from 55 patients with cholelithiasis, 18 with bile duct or gallbladder cancer and 19 with pancreatic cancer were studied. Sera were obtained from 34 control subjects who underwent endoscopy and were diagnosed as not having peptic ulcers or cancers. Seropositivity of H. hepaticus was examined by western blot analysis using a H. hepaticus‐specific antigen. To validate the specificity, positive sera were also tested after absorption with H. hepaticus whole‐cell sonicate. Serum samples were also tested for the presence of anti‐Helicobacter pylori antibody.
Results: Prevalence of antibody to H. hepaticus‐specific antigen in patients with bile tract cancer was 38.8% and was significantly higher than in control subjects (13.1%, P < 0.05). Prevalence of antibody to H. hepaticus‐specific antigen was 18.2% and 10.5% in patients with cholelithiasis and pancreatic cancer, respectively. Seropositivity for H. pylori was similar in all groups. Detection of the H. hepaticus‐specific band was significantly decreased after the sera were absorbed with H. hepaticus whole‐cell sonicate.
Conclusion: Infection with H. hepaticus might be associated with bile duct cancer. Results obtained from absorbed sera suggested high specificity of the western blot analysis.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>20586873</pmid><doi>10.1111/j.1440-1746.2010.06224.x</doi><tpages>4</tpages></addata></record> |
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| subjects | Aged Aged, 80 and over Antibodies, Bacterial - blood Bile Duct Neoplasms - blood Bile Duct Neoplasms - epidemiology biliary diseases Biliary Tract Diseases - blood Biliary Tract Diseases - epidemiology Biological and medical sciences Biomarkers - blood Blotting, Western Case-Control Studies Chi-Square Distribution Cholelithiasis - blood Cholelithiasis - epidemiology Female Gallbladder Neoplasms - blood Gallbladder Neoplasms - epidemiology Gastroenterology. Liver. Pancreas. Abdomen Helicobacter hepaticus Helicobacter hepaticus - immunology Helicobacter Infections - blood Helicobacter Infections - epidemiology Helicobacter Infections - microbiology Humans Japan - epidemiology Male Medical sciences Middle Aged Pancreatic Neoplasms - blood Pancreatic Neoplasms - epidemiology serodiagnosis Seroepidemiologic Studies |
| title | Serological analysis of Helicobacter hepaticus infection in patients with biliary and pancreatic diseases |
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