Cutaneous distribution of plasmacytoid dendritic cells in lupus erythematosus. Selective tropism at the site of epithelial apoptotic damage
Abstract Recent evidences suggest a significant role of Plasmacytoid dendritic cells (PDC) role in the pathogenesis of lupus erythematosus (LE) via production of type I IFN. Taking advantage on the availability of multiple reagents (CD123, BDCA2, and CD2ap) specifically recognizing PDC on fixed tiss...
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| Veröffentlicht in: | Immunobiology (1979) 2009-09, Vol.214 (9), p.877-886 |
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| creator | Vermi, William Lonardi, Silvia Morassi, Mauro Rossini, Cristina Tardanico, Regina Venturini, Marina Sala, Raffaella Tincani, Angela Poliani, Pietro Luigi Calzavara-Pinton, Pier Giacomo Cerroni, Lorenzo Santoro, Amerigo Facchetti, Fabio |
| description | Abstract Recent evidences suggest a significant role of Plasmacytoid dendritic cells (PDC) role in the pathogenesis of lupus erythematosus (LE) via production of type I IFN. Taking advantage on the availability of multiple reagents (CD123, BDCA2, and CD2ap) specifically recognizing PDC on fixed tissues, we investigated the occurrence of PDC in a cohort of 74 LE patients. The large majority of LE biopsies (67/74; 90.5%) showed cutaneous infiltration of PDC. PDC were more frequently observed (96.4 vs 72.2) and numerous in cutaneous LE compared to systemic LE (SLE) and correlated with the density of the inflammatory infiltrate ( r =0.40; p |
| doi_str_mv | 10.1016/j.imbio.2009.06.013 |
| format | Article |
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Selective tropism at the site of epithelial apoptotic damage</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Vermi, William ; Lonardi, Silvia ; Morassi, Mauro ; Rossini, Cristina ; Tardanico, Regina ; Venturini, Marina ; Sala, Raffaella ; Tincani, Angela ; Poliani, Pietro Luigi ; Calzavara-Pinton, Pier Giacomo ; Cerroni, Lorenzo ; Santoro, Amerigo ; Facchetti, Fabio</creator><creatorcontrib>Vermi, William ; Lonardi, Silvia ; Morassi, Mauro ; Rossini, Cristina ; Tardanico, Regina ; Venturini, Marina ; Sala, Raffaella ; Tincani, Angela ; Poliani, Pietro Luigi ; Calzavara-Pinton, Pier Giacomo ; Cerroni, Lorenzo ; Santoro, Amerigo ; Facchetti, Fabio</creatorcontrib><description>Abstract Recent evidences suggest a significant role of Plasmacytoid dendritic cells (PDC) role in the pathogenesis of lupus erythematosus (LE) via production of type I IFN. Taking advantage on the availability of multiple reagents (CD123, BDCA2, and CD2ap) specifically recognizing PDC on fixed tissues, we investigated the occurrence of PDC in a cohort of 74 LE patients. The large majority of LE biopsies (67/74; 90.5%) showed cutaneous infiltration of PDC. PDC were more frequently observed (96.4 vs 72.2) and numerous in cutaneous LE compared to systemic LE (SLE) and correlated with the density of the inflammatory infiltrate ( r =0.40; p <0.001). PDC reduction in SLE might be related to a broader tissue distribution of this cellular population, as indicated by their occurrence in kidneys in 11 out of 24 (45.8%) cases studied. The distribution of cutaneous PDC showed two distinct patterns. More commonly, PDC were observed within perivascular inflammatory nodules in the dermis, associated with CD208+ mature DC and T-bet+ cells [D-PDC]. A second component was observed along the dermal-epithelial junction [J-PDC], in association with cytotoxic T-cells in areas of severe epithelial damage. Notably, chemerin reactivity was observed in 64% of LE biopsies on endothelial cells and in the granular layer keratinocytes. Cutaneous PDC in LE strongly produced type I IFN, as indicated by the diffuse MxA expression, and the cytotoxic molecule granzyme B. This study confirms cutaneous PDC infiltration as hallmark of LE. The topographical segregation in D-PDC and J-PDC suggests a novel view of the role of these cells in skin autoimmunity.</description><identifier>ISSN: 0171-2985</identifier><identifier>EISSN: 1878-3279</identifier><identifier>DOI: 10.1016/j.imbio.2009.06.013</identifier><identifier>PMID: 19625100</identifier><language>eng</language><publisher>Netherlands: Elsevier GmbH</publisher><subject>Advanced Basic Science ; Allergy and Immunology ; Apoptosis ; Cell Movement ; Chimerin Proteins - metabolism ; Cutaneous ; Dendritic Cells - immunology ; Dermis - immunology ; Dermis - pathology ; Humans ; Immunohistochemistry ; Interferon ; Interferon Type I - biosynthesis ; Kidney - immunology ; Kidney - pathology ; Lupus erythematosus ; Lupus Erythematosus, Cutaneous - immunology ; Lupus Erythematosus, Cutaneous - pathology ; Lysosomal Membrane Proteins - metabolism ; Neoplasm Proteins - metabolism ; Plasmacytoid dendritic cell ; Skin - immunology ; Skin - pathology ; T-Box Domain Proteins - metabolism</subject><ispartof>Immunobiology (1979), 2009-09, Vol.214 (9), p.877-886</ispartof><rights>Elsevier GmbH</rights><rights>2009 Elsevier GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-f2e10fdaa92c05206e86036f33825dcf0185babc07ce922cb6c7cf8fa7bd83473</citedby><cites>FETCH-LOGICAL-c510t-f2e10fdaa92c05206e86036f33825dcf0185babc07ce922cb6c7cf8fa7bd83473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.imbio.2009.06.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19625100$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vermi, William</creatorcontrib><creatorcontrib>Lonardi, Silvia</creatorcontrib><creatorcontrib>Morassi, Mauro</creatorcontrib><creatorcontrib>Rossini, Cristina</creatorcontrib><creatorcontrib>Tardanico, Regina</creatorcontrib><creatorcontrib>Venturini, Marina</creatorcontrib><creatorcontrib>Sala, Raffaella</creatorcontrib><creatorcontrib>Tincani, Angela</creatorcontrib><creatorcontrib>Poliani, Pietro Luigi</creatorcontrib><creatorcontrib>Calzavara-Pinton, Pier Giacomo</creatorcontrib><creatorcontrib>Cerroni, Lorenzo</creatorcontrib><creatorcontrib>Santoro, Amerigo</creatorcontrib><creatorcontrib>Facchetti, Fabio</creatorcontrib><title>Cutaneous distribution of plasmacytoid dendritic cells in lupus erythematosus. Selective tropism at the site of epithelial apoptotic damage</title><title>Immunobiology (1979)</title><addtitle>Immunobiology</addtitle><description>Abstract Recent evidences suggest a significant role of Plasmacytoid dendritic cells (PDC) role in the pathogenesis of lupus erythematosus (LE) via production of type I IFN. Taking advantage on the availability of multiple reagents (CD123, BDCA2, and CD2ap) specifically recognizing PDC on fixed tissues, we investigated the occurrence of PDC in a cohort of 74 LE patients. The large majority of LE biopsies (67/74; 90.5%) showed cutaneous infiltration of PDC. PDC were more frequently observed (96.4 vs 72.2) and numerous in cutaneous LE compared to systemic LE (SLE) and correlated with the density of the inflammatory infiltrate ( r =0.40; p <0.001). PDC reduction in SLE might be related to a broader tissue distribution of this cellular population, as indicated by their occurrence in kidneys in 11 out of 24 (45.8%) cases studied. The distribution of cutaneous PDC showed two distinct patterns. More commonly, PDC were observed within perivascular inflammatory nodules in the dermis, associated with CD208+ mature DC and T-bet+ cells [D-PDC]. A second component was observed along the dermal-epithelial junction [J-PDC], in association with cytotoxic T-cells in areas of severe epithelial damage. Notably, chemerin reactivity was observed in 64% of LE biopsies on endothelial cells and in the granular layer keratinocytes. Cutaneous PDC in LE strongly produced type I IFN, as indicated by the diffuse MxA expression, and the cytotoxic molecule granzyme B. This study confirms cutaneous PDC infiltration as hallmark of LE. The topographical segregation in D-PDC and J-PDC suggests a novel view of the role of these cells in skin autoimmunity.</description><subject>Advanced Basic Science</subject><subject>Allergy and Immunology</subject><subject>Apoptosis</subject><subject>Cell Movement</subject><subject>Chimerin Proteins - metabolism</subject><subject>Cutaneous</subject><subject>Dendritic Cells - immunology</subject><subject>Dermis - immunology</subject><subject>Dermis - pathology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Interferon</subject><subject>Interferon Type I - biosynthesis</subject><subject>Kidney - immunology</subject><subject>Kidney - pathology</subject><subject>Lupus erythematosus</subject><subject>Lupus Erythematosus, Cutaneous - immunology</subject><subject>Lupus Erythematosus, Cutaneous - pathology</subject><subject>Lysosomal Membrane Proteins - metabolism</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Plasmacytoid dendritic cell</subject><subject>Skin - immunology</subject><subject>Skin - pathology</subject><subject>T-Box Domain Proteins - metabolism</subject><issn>0171-2985</issn><issn>1878-3279</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk-L1TAUxYsozpvRTyBIVrpqzR-bpAsFeTgqDLgYBXchTW71PtumJunA-wx-aVPfA8GFrkLgd05yz7lV9YTRhlEmXxwanHoMDae0a6hsKBP3qh3TSteCq-5-taNMsZp3ur2oLlM6UMo6rvTD6oJ1kreM0l31c79mO0NYE_GYcsR-zRhmEgayjDZN1h1zQE88zD5iRkccjGMiOJNxXYoK4jF_g8nmkNbUkFsYwWW8A5JjWDBNxGZSAJIww-YKC5briHYkdglLDpunt5P9Co-qB4MdEzw-n1fV5-u3n_bv65uP7z7s39zUrvw51wMHRgdvbccdbTmVoCUVchBC89a7gTLd9rZ3VDnoOHe9dMoNerCq91q8VOKqen7yXWL4sULKZsK0jXUKwighOqlbKQv57J8kZ0y0Wm2W4gS6GFKKMJgl4mTj0TBqtrbMwfxuy2xtGSpNaauonp7t134C_0dzrqcAr04AlDjuEKJJDmF24DGWmI0P-J8HXv-ldyPO6Oz4HY6QDmGNc0naMJO4oeZ2W5htX2hXdoWpL-IX7Ku_xQ</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Vermi, William</creator><creator>Lonardi, Silvia</creator><creator>Morassi, Mauro</creator><creator>Rossini, Cristina</creator><creator>Tardanico, Regina</creator><creator>Venturini, Marina</creator><creator>Sala, Raffaella</creator><creator>Tincani, Angela</creator><creator>Poliani, Pietro Luigi</creator><creator>Calzavara-Pinton, Pier Giacomo</creator><creator>Cerroni, Lorenzo</creator><creator>Santoro, Amerigo</creator><creator>Facchetti, Fabio</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090901</creationdate><title>Cutaneous distribution of plasmacytoid dendritic cells in lupus erythematosus. Selective tropism at the site of epithelial apoptotic damage</title><author>Vermi, William ; Lonardi, Silvia ; Morassi, Mauro ; Rossini, Cristina ; Tardanico, Regina ; Venturini, Marina ; Sala, Raffaella ; Tincani, Angela ; Poliani, Pietro Luigi ; Calzavara-Pinton, Pier Giacomo ; Cerroni, Lorenzo ; Santoro, Amerigo ; Facchetti, Fabio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-f2e10fdaa92c05206e86036f33825dcf0185babc07ce922cb6c7cf8fa7bd83473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Advanced Basic Science</topic><topic>Allergy and Immunology</topic><topic>Apoptosis</topic><topic>Cell Movement</topic><topic>Chimerin Proteins - metabolism</topic><topic>Cutaneous</topic><topic>Dendritic Cells - immunology</topic><topic>Dermis - immunology</topic><topic>Dermis - pathology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Interferon</topic><topic>Interferon Type I - biosynthesis</topic><topic>Kidney - immunology</topic><topic>Kidney - pathology</topic><topic>Lupus erythematosus</topic><topic>Lupus Erythematosus, Cutaneous - immunology</topic><topic>Lupus Erythematosus, Cutaneous - pathology</topic><topic>Lysosomal Membrane Proteins - metabolism</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Plasmacytoid dendritic cell</topic><topic>Skin - immunology</topic><topic>Skin - pathology</topic><topic>T-Box Domain Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vermi, William</creatorcontrib><creatorcontrib>Lonardi, Silvia</creatorcontrib><creatorcontrib>Morassi, Mauro</creatorcontrib><creatorcontrib>Rossini, Cristina</creatorcontrib><creatorcontrib>Tardanico, Regina</creatorcontrib><creatorcontrib>Venturini, Marina</creatorcontrib><creatorcontrib>Sala, Raffaella</creatorcontrib><creatorcontrib>Tincani, Angela</creatorcontrib><creatorcontrib>Poliani, Pietro Luigi</creatorcontrib><creatorcontrib>Calzavara-Pinton, Pier Giacomo</creatorcontrib><creatorcontrib>Cerroni, Lorenzo</creatorcontrib><creatorcontrib>Santoro, Amerigo</creatorcontrib><creatorcontrib>Facchetti, Fabio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunobiology (1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vermi, William</au><au>Lonardi, Silvia</au><au>Morassi, Mauro</au><au>Rossini, Cristina</au><au>Tardanico, Regina</au><au>Venturini, Marina</au><au>Sala, Raffaella</au><au>Tincani, Angela</au><au>Poliani, Pietro Luigi</au><au>Calzavara-Pinton, Pier Giacomo</au><au>Cerroni, Lorenzo</au><au>Santoro, Amerigo</au><au>Facchetti, Fabio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cutaneous distribution of plasmacytoid dendritic cells in lupus erythematosus. Selective tropism at the site of epithelial apoptotic damage</atitle><jtitle>Immunobiology (1979)</jtitle><addtitle>Immunobiology</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>214</volume><issue>9</issue><spage>877</spage><epage>886</epage><pages>877-886</pages><issn>0171-2985</issn><eissn>1878-3279</eissn><abstract>Abstract Recent evidences suggest a significant role of Plasmacytoid dendritic cells (PDC) role in the pathogenesis of lupus erythematosus (LE) via production of type I IFN. Taking advantage on the availability of multiple reagents (CD123, BDCA2, and CD2ap) specifically recognizing PDC on fixed tissues, we investigated the occurrence of PDC in a cohort of 74 LE patients. The large majority of LE biopsies (67/74; 90.5%) showed cutaneous infiltration of PDC. PDC were more frequently observed (96.4 vs 72.2) and numerous in cutaneous LE compared to systemic LE (SLE) and correlated with the density of the inflammatory infiltrate ( r =0.40; p <0.001). PDC reduction in SLE might be related to a broader tissue distribution of this cellular population, as indicated by their occurrence in kidneys in 11 out of 24 (45.8%) cases studied. The distribution of cutaneous PDC showed two distinct patterns. More commonly, PDC were observed within perivascular inflammatory nodules in the dermis, associated with CD208+ mature DC and T-bet+ cells [D-PDC]. A second component was observed along the dermal-epithelial junction [J-PDC], in association with cytotoxic T-cells in areas of severe epithelial damage. Notably, chemerin reactivity was observed in 64% of LE biopsies on endothelial cells and in the granular layer keratinocytes. Cutaneous PDC in LE strongly produced type I IFN, as indicated by the diffuse MxA expression, and the cytotoxic molecule granzyme B. This study confirms cutaneous PDC infiltration as hallmark of LE. The topographical segregation in D-PDC and J-PDC suggests a novel view of the role of these cells in skin autoimmunity.</abstract><cop>Netherlands</cop><pub>Elsevier GmbH</pub><pmid>19625100</pmid><doi>10.1016/j.imbio.2009.06.013</doi><tpages>10</tpages></addata></record> |
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| ispartof | Immunobiology (1979), 2009-09, Vol.214 (9), p.877-886 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Advanced Basic Science Allergy and Immunology Apoptosis Cell Movement Chimerin Proteins - metabolism Cutaneous Dendritic Cells - immunology Dermis - immunology Dermis - pathology Humans Immunohistochemistry Interferon Interferon Type I - biosynthesis Kidney - immunology Kidney - pathology Lupus erythematosus Lupus Erythematosus, Cutaneous - immunology Lupus Erythematosus, Cutaneous - pathology Lysosomal Membrane Proteins - metabolism Neoplasm Proteins - metabolism Plasmacytoid dendritic cell Skin - immunology Skin - pathology T-Box Domain Proteins - metabolism |
| title | Cutaneous distribution of plasmacytoid dendritic cells in lupus erythematosus. Selective tropism at the site of epithelial apoptotic damage |
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