Two subforms of eukaryotic topoisomerase I Purification and structure-function relationships

A new method for isolation of eukaryotic topoisomerase 1 from calf thymus and from Jurkat-1 cells using HPLC has been developed. The method allows quantitative purification of high molecular weight topo I and of two low molecular weight fractions differing by their isoelectric points. It has been su...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	FEBS letters 1992-12, Vol.314 (3), p.267-270
Hauptverfasser:	Kudinov, Alexei R., Bronstein, Igor B., Gabibov, Alexander G., Gololobov, Gennady V.
Format:	Artikel
Sprache:	eng
Schlagworte:	2-mercaptoethanol ammonium sulfate Animals camptothecin Camptothecin resistance Cattle Cell Line Chromatography, High Pressure Liquid CPT DNA Topoisomerases, Type I - chemistry DNA Topoisomerases, Type I - isolation & purification DNA Topoisomerases, Type I - metabolism DNA, Bacterial - metabolism Electrophoresis, Polyacrylamide Gel high performance liquid chromatography HPLC HPLC, Hydrophobic interaction chromatography Humans KPB phenylmethylsulfonylfluoride Plasmids PMSF potassium phosphate buffer Structure-Activity Relationship Subform topo I of 130–145, 66, 70-75, 72 kDa, respectively topo I/130–145, 66, 70–75, 72 Topoisomerase I
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!