A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies
A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of th...
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| Veröffentlicht in: | Clinica chimica acta 1992-10, Vol.211 (1), p.59-72 |
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| container_title | Clinica chimica acta |
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| creator | Obata, Ken'ichi Iwata, Kazushi Okada, Yasunori Kohrin, Yumi Ohuchi, Eiko Yoshida, Shin'ichi Shinmei, Masayuki Hayakawa, Taro |
| description | A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay system was 20 μ/l and linearity was obtained between 31 and 500 μg/l. The EIA system was capable of measuring both precursor and active forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of metalloproteinases. MMP-3 levels as measured by this assay are significantly higher in the sera of patients with rheumatoid arthritis as compared to those of healthy subjects and patients with osteoarthritis. Immunoblot analyses showed that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 is present in the 59- and 57-kDa precursor forms. |
| doi_str_mv | 10.1016/0009-8981(92)90105-Y |
| format | Article |
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The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay system was 20 μ/l and linearity was obtained between 31 and 500 μg/l. The EIA system was capable of measuring both precursor and active forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of metalloproteinases. MMP-3 levels as measured by this assay are significantly higher in the sera of patients with rheumatoid arthritis as compared to those of healthy subjects and patients with osteoarthritis. Immunoblot analyses showed that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 is present in the 59- and 57-kDa precursor forms.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(92)90105-Y</identifier><identifier>PMID: 1281763</identifier><identifier>CODEN: CCATAR</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>alpha-Macroglobulins - metabolism ; Analytical, structural and metabolic biochemistry ; Animals ; Antibodies, Monoclonal ; Antibody Specificity ; Biological and medical sciences ; Cell Line ; Enzymes and enzyme inhibitors ; Female ; Fundamental and applied biological sciences. Psychology ; Glycoproteins - metabolism ; Humans ; Hydrolases ; Immunoenzyme Techniques - standards ; Immunoenzyme Techniques - statistics & numerical data ; Matrix Metalloproteinase 3 ; Metalloendopeptidases - analysis ; Metalloendopeptidases - blood ; Metalloendopeptidases - immunology ; Mice ; Mice, Inbred BALB C ; Monoclonal antibody ; Neoplasm Proteins - metabolism ; Osteoarthritis ; Protein Precursors - analysis ; Protein Precursors - blood ; Protein Precursors - immunology ; Rheumatoid arthritis ; Sandwich enzyme immunoassay ; Stromelysin-1 ; Synovial Fluid - chemistry ; Tissue Inhibitor of Metalloproteinase-2 ; Tissue Inhibitor of Metalloproteinases</subject><ispartof>Clinica chimica acta, 1992-10, Vol.211 (1), p.59-72</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-2dce44b7f600c9047c32d00014cb3cca3b4f5d2a1de1f408e5679b050cccb0b43</citedby><cites>FETCH-LOGICAL-c452t-2dce44b7f600c9047c32d00014cb3cca3b4f5d2a1de1f408e5679b050cccb0b43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0009-8981(92)90105-Y$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4495916$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1281763$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Obata, Ken'ichi</creatorcontrib><creatorcontrib>Iwata, Kazushi</creatorcontrib><creatorcontrib>Okada, Yasunori</creatorcontrib><creatorcontrib>Kohrin, Yumi</creatorcontrib><creatorcontrib>Ohuchi, Eiko</creatorcontrib><creatorcontrib>Yoshida, Shin'ichi</creatorcontrib><creatorcontrib>Shinmei, Masayuki</creatorcontrib><creatorcontrib>Hayakawa, Taro</creatorcontrib><title>A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay system was 20 μ/l and linearity was obtained between 31 and 500 μg/l. The EIA system was capable of measuring both precursor and active forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of metalloproteinases. MMP-3 levels as measured by this assay are significantly higher in the sera of patients with rheumatoid arthritis as compared to those of healthy subjects and patients with osteoarthritis. Immunoblot analyses showed that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 is present in the 59- and 57-kDa precursor forms.</description><subject>alpha-Macroglobulins - metabolism</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins - metabolism</subject><subject>Humans</subject><subject>Hydrolases</subject><subject>Immunoenzyme Techniques - standards</subject><subject>Immunoenzyme Techniques - statistics & numerical data</subject><subject>Matrix Metalloproteinase 3</subject><subject>Metalloendopeptidases - analysis</subject><subject>Metalloendopeptidases - blood</subject><subject>Metalloendopeptidases - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Monoclonal antibody</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Osteoarthritis</subject><subject>Protein Precursors - analysis</subject><subject>Protein Precursors - blood</subject><subject>Protein Precursors - immunology</subject><subject>Rheumatoid arthritis</subject><subject>Sandwich enzyme immunoassay</subject><subject>Stromelysin-1</subject><subject>Synovial Fluid - chemistry</subject><subject>Tissue Inhibitor of Metalloproteinase-2</subject><subject>Tissue Inhibitor of Metalloproteinases</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1vFDEMhiMEKtvCPwApB4Taw0AyyXzkglRVFJAqcYFDT1HG46FBk2SJZyjLryfLrsqN0yvLjy37YeyFFG-kkO1bIYSpetPLc1NfGCFFU90-YhvZd6pS2tSP2eYBecpOib6XUotWnrATWfeya9WG3V_yFLGiBbecXBzvPdxxjL93AbkPYY3JEbkdn1Lmd2twkQe3ZP-LB1zcPKdtTgv66Ai54ue05BRw3pGPlbzga8lvPKSYYE7RzdzFxQ9p9EjP2JPJzYTPj3nGvl6__3L1sbr5_OHT1eVNBbqpl6oeAbUeuqkVAozQHah6LG9IDYMCcGrQUzPWTo4oJy16bNrODKIRADCIQasz9vqwtxz6Y0VabPAEOM8uYlrJdkqZthaygPoAQk5EGSe7zT64vLNS2L1vu5dp9zKtqe1f3_a2jL087l-HgOO_oYPg0n917DsCN0_ZRfD0gGltGiPbgr07YFhc_PSYLYHHCDj6jLDYMfn_3_EHjMaeeQ</recordid><startdate>19921015</startdate><enddate>19921015</enddate><creator>Obata, Ken'ichi</creator><creator>Iwata, Kazushi</creator><creator>Okada, Yasunori</creator><creator>Kohrin, Yumi</creator><creator>Ohuchi, Eiko</creator><creator>Yoshida, Shin'ichi</creator><creator>Shinmei, Masayuki</creator><creator>Hayakawa, Taro</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921015</creationdate><title>A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies</title><author>Obata, Ken'ichi ; Iwata, Kazushi ; Okada, Yasunori ; Kohrin, Yumi ; Ohuchi, Eiko ; Yoshida, Shin'ichi ; Shinmei, Masayuki ; Hayakawa, Taro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-2dce44b7f600c9047c32d00014cb3cca3b4f5d2a1de1f408e5679b050cccb0b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>alpha-Macroglobulins - metabolism</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins - metabolism</topic><topic>Humans</topic><topic>Hydrolases</topic><topic>Immunoenzyme Techniques - standards</topic><topic>Immunoenzyme Techniques - statistics & numerical data</topic><topic>Matrix Metalloproteinase 3</topic><topic>Metalloendopeptidases - analysis</topic><topic>Metalloendopeptidases - blood</topic><topic>Metalloendopeptidases - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Monoclonal antibody</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Osteoarthritis</topic><topic>Protein Precursors - analysis</topic><topic>Protein Precursors - blood</topic><topic>Protein Precursors - immunology</topic><topic>Rheumatoid arthritis</topic><topic>Sandwich enzyme immunoassay</topic><topic>Stromelysin-1</topic><topic>Synovial Fluid - chemistry</topic><topic>Tissue Inhibitor of Metalloproteinase-2</topic><topic>Tissue Inhibitor of Metalloproteinases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Obata, Ken'ichi</creatorcontrib><creatorcontrib>Iwata, Kazushi</creatorcontrib><creatorcontrib>Okada, Yasunori</creatorcontrib><creatorcontrib>Kohrin, Yumi</creatorcontrib><creatorcontrib>Ohuchi, Eiko</creatorcontrib><creatorcontrib>Yoshida, Shin'ichi</creatorcontrib><creatorcontrib>Shinmei, Masayuki</creatorcontrib><creatorcontrib>Hayakawa, Taro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Obata, Ken'ichi</au><au>Iwata, Kazushi</au><au>Okada, Yasunori</au><au>Kohrin, Yumi</au><au>Ohuchi, Eiko</au><au>Yoshida, Shin'ichi</au><au>Shinmei, Masayuki</au><au>Hayakawa, Taro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1992-10-15</date><risdate>1992</risdate><volume>211</volume><issue>1</issue><spage>59</spage><epage>72</epage><pages>59-72</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>A one-step sandwich enzyme immunoassay (EIA) for matrix metalloproteinase 3 (MMP-3; stromelysin-1) was developed. The assay system used two simultaneous immunoreactions using a solid phase monoclonal antibody and a horseradish peroxidase-labeled monoclonal antibody (Fab'). The sensitivity of the assay system was 20 μ/l and linearity was obtained between 31 and 500 μg/l. The EIA system was capable of measuring both precursor and active forms of MMP-3 as well as the forms of MMP-3 complexed with tissue inhibitors of metalloproteinases. MMP-3 levels as measured by this assay are significantly higher in the sera of patients with rheumatoid arthritis as compared to those of healthy subjects and patients with osteoarthritis. Immunoblot analyses showed that in the sera and synovial fluids of patients with rheumatoid arthritis, MMP-3 is present in the 59- and 57-kDa precursor forms.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>1281763</pmid><doi>10.1016/0009-8981(92)90105-Y</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Clinica chimica acta, 1992-10, Vol.211 (1), p.59-72 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | alpha-Macroglobulins - metabolism Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal Antibody Specificity Biological and medical sciences Cell Line Enzymes and enzyme inhibitors Female Fundamental and applied biological sciences. Psychology Glycoproteins - metabolism Humans Hydrolases Immunoenzyme Techniques - standards Immunoenzyme Techniques - statistics & numerical data Matrix Metalloproteinase 3 Metalloendopeptidases - analysis Metalloendopeptidases - blood Metalloendopeptidases - immunology Mice Mice, Inbred BALB C Monoclonal antibody Neoplasm Proteins - metabolism Osteoarthritis Protein Precursors - analysis Protein Precursors - blood Protein Precursors - immunology Rheumatoid arthritis Sandwich enzyme immunoassay Stromelysin-1 Synovial Fluid - chemistry Tissue Inhibitor of Metalloproteinase-2 Tissue Inhibitor of Metalloproteinases |
| title | A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 3 (stromelysin-1) using monoclonal antibodies |
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