Rapid determination of methylxanthines in real samples by high-performance liquid chromatography using the new FastGradient ® narrow-bore monolithic column
The present study reports one of the very first analytical applications of the new narrow-bore monolithic column, FastGradient ® Chromolith (50 mm × 2.0 mm i.d.). The three major methylxanthines (theobromine, theophylline and caffeine) were separated rapidly and determined simultaneously in various...
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| Veröffentlicht in: | Talanta (Oxford) 2010-06, Vol.81 (4), p.1494-1501 |
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| creator | Tzanavaras, Paraskevas D. Zacharis, Constantinos K. Themelis, Demetrius G. |
| description | The present study reports one of the very first analytical applications of the new narrow-bore monolithic column, FastGradient
® Chromolith (50
mm
×
2.0
mm i.d.). The three major methylxanthines (theobromine, theophylline and caffeine) were separated rapidly and determined simultaneously in various real samples. Based on the unique characteristics of this novel monolithic column the analytes were separated efficiently (
R
s
>
3.0) in less than 5
min at a low flow rate of 0.5
mL
min
−1 and using a low volume fraction of organic solvent (5% acetonitrile (ACN) in water) in the mobile phase. UV detection was carried out at 274
nm. The separation was optimized in terms of mobile phase composition, flow rate and injection volume, while the method was validated for linearity, detection and quantitation limits, within and day-to-day precision, accuracy and ruggedness. Its applicability was demonstrated by analyzing a variety of real samples including beverages, soft drinks, herbal products and pharmaceuticals. Compared to a well-established monolithic (Performance Chromolith 100
mm
×
4.6
mm i.d.) and a particulate reversed phase column (Hypersil ODS 5
μm 150
mm
×
4.6
mm i.d.), the narrow-bore FastGradient
® column offered satisfactory performance, faster analysis time and drastic reduction in the consumption of mobile phase and organic solvents. |
| doi_str_mv | 10.1016/j.talanta.2010.02.058 |
| format | Article |
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® Chromolith (50
mm
×
2.0
mm i.d.). The three major methylxanthines (theobromine, theophylline and caffeine) were separated rapidly and determined simultaneously in various real samples. Based on the unique characteristics of this novel monolithic column the analytes were separated efficiently (
R
s
>
3.0) in less than 5
min at a low flow rate of 0.5
mL
min
−1 and using a low volume fraction of organic solvent (5% acetonitrile (ACN) in water) in the mobile phase. UV detection was carried out at 274
nm. The separation was optimized in terms of mobile phase composition, flow rate and injection volume, while the method was validated for linearity, detection and quantitation limits, within and day-to-day precision, accuracy and ruggedness. Its applicability was demonstrated by analyzing a variety of real samples including beverages, soft drinks, herbal products and pharmaceuticals. Compared to a well-established monolithic (Performance Chromolith 100
mm
×
4.6
mm i.d.) and a particulate reversed phase column (Hypersil ODS 5
μm 150
mm
×
4.6
mm i.d.), the narrow-bore FastGradient
® column offered satisfactory performance, faster analysis time and drastic reduction in the consumption of mobile phase and organic solvents.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2010.02.058</identifier><identifier>PMID: 20441929</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Beverages - analysis ; Caffeine ; Caffeine - analysis ; Carbonated Beverages - analysis ; Chemistry ; Chemistry Techniques, Analytical ; Chromatographic methods and physical methods associated with chromatography ; Chromatography, High Pressure Liquid - methods ; Coffee ; Exact sciences and technology ; FastGradient ® monolithic column ; Food Analysis - methods ; Food Contamination ; High-performance liquid chromatography ; Limit of Detection ; Other chromatographic methods ; Pharmaceutical Preparations - analysis ; Reproducibility of Results ; Tablets ; Tea ; Theobromine ; Theobromine - analysis ; Theophylline ; Theophylline - analysis ; Water - chemistry ; Xanthines - analysis</subject><ispartof>Talanta (Oxford), 2010-06, Vol.81 (4), p.1494-1501</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-94309ba84f9d510a34d73ad17d437d3662b35e0e80b41012b28347b3404f81aa3</citedby><cites>FETCH-LOGICAL-c394t-94309ba84f9d510a34d73ad17d437d3662b35e0e80b41012b28347b3404f81aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0039914010001736$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22797918$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20441929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tzanavaras, Paraskevas D.</creatorcontrib><creatorcontrib>Zacharis, Constantinos K.</creatorcontrib><creatorcontrib>Themelis, Demetrius G.</creatorcontrib><title>Rapid determination of methylxanthines in real samples by high-performance liquid chromatography using the new FastGradient ® narrow-bore monolithic column</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>The present study reports one of the very first analytical applications of the new narrow-bore monolithic column, FastGradient
® Chromolith (50
mm
×
2.0
mm i.d.). The three major methylxanthines (theobromine, theophylline and caffeine) were separated rapidly and determined simultaneously in various real samples. Based on the unique characteristics of this novel monolithic column the analytes were separated efficiently (
R
s
>
3.0) in less than 5
min at a low flow rate of 0.5
mL
min
−1 and using a low volume fraction of organic solvent (5% acetonitrile (ACN) in water) in the mobile phase. UV detection was carried out at 274
nm. The separation was optimized in terms of mobile phase composition, flow rate and injection volume, while the method was validated for linearity, detection and quantitation limits, within and day-to-day precision, accuracy and ruggedness. Its applicability was demonstrated by analyzing a variety of real samples including beverages, soft drinks, herbal products and pharmaceuticals. Compared to a well-established monolithic (Performance Chromolith 100
mm
×
4.6
mm i.d.) and a particulate reversed phase column (Hypersil ODS 5
μm 150
mm
×
4.6
mm i.d.), the narrow-bore FastGradient
® column offered satisfactory performance, faster analysis time and drastic reduction in the consumption of mobile phase and organic solvents.</description><subject>Analytical chemistry</subject><subject>Beverages - analysis</subject><subject>Caffeine</subject><subject>Caffeine - analysis</subject><subject>Carbonated Beverages - analysis</subject><subject>Chemistry</subject><subject>Chemistry Techniques, Analytical</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Coffee</subject><subject>Exact sciences and technology</subject><subject>FastGradient ® monolithic column</subject><subject>Food Analysis - methods</subject><subject>Food Contamination</subject><subject>High-performance liquid chromatography</subject><subject>Limit of Detection</subject><subject>Other chromatographic methods</subject><subject>Pharmaceutical Preparations - analysis</subject><subject>Reproducibility of Results</subject><subject>Tablets</subject><subject>Tea</subject><subject>Theobromine</subject><subject>Theobromine - analysis</subject><subject>Theophylline</subject><subject>Theophylline - analysis</subject><subject>Water - chemistry</subject><subject>Xanthines - analysis</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAURSMEokPhE0DeoK4ytWNnkqwQqmhBqlQJwdp6sV8mHsV2ajst8y_9Bj6CL8OjGWDJ6slP591r3VsUbxldM8o2l7t1gglcgnVF845Wa1q3z4oVaxte8rrhz4sVpbwrOyboWfEqxh2ltOKUvyzOKioE66puVTx9hdloojFhsMZBMt4RPxCLadxPP7LBaBxGYhwJCBOJYOcpv_s9Gc12LGcMgw8WnEIymfsla6kxeAvJbwPM454s0bgtSSMSh4_kGmK6CaANukR-_SQOQvCPZe8DEuudn0w2VET5abHudfFigCnim9M8L75ff_p29bm8vbv5cvXxtlS8E6nsBKddD60YOl0zClzohoNmjRa80XyzqXpeI8WW9iJnV_VVy0XTc0HF0DIAfl5cHHXn4O8XjElaExVOOV_0S5QNzz7tpq0zWR9JFXyMAQc5B2Mh7CWj8tCL3MlTL_LQi6SVzL3ku3cnh6W3qP9e_SkiA-9PAEQF0xByoib-46qmazp2EPpw5DDn8WAwyKhylgq1CaiS1N785yu_AYjIsj8</recordid><startdate>20100615</startdate><enddate>20100615</enddate><creator>Tzanavaras, Paraskevas D.</creator><creator>Zacharis, Constantinos K.</creator><creator>Themelis, Demetrius G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100615</creationdate><title>Rapid determination of methylxanthines in real samples by high-performance liquid chromatography using the new FastGradient ® narrow-bore monolithic column</title><author>Tzanavaras, Paraskevas D. ; Zacharis, Constantinos K. ; Themelis, Demetrius G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-94309ba84f9d510a34d73ad17d437d3662b35e0e80b41012b28347b3404f81aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytical chemistry</topic><topic>Beverages - analysis</topic><topic>Caffeine</topic><topic>Caffeine - analysis</topic><topic>Carbonated Beverages - analysis</topic><topic>Chemistry</topic><topic>Chemistry Techniques, Analytical</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Coffee</topic><topic>Exact sciences and technology</topic><topic>FastGradient ® monolithic column</topic><topic>Food Analysis - methods</topic><topic>Food Contamination</topic><topic>High-performance liquid chromatography</topic><topic>Limit of Detection</topic><topic>Other chromatographic methods</topic><topic>Pharmaceutical Preparations - analysis</topic><topic>Reproducibility of Results</topic><topic>Tablets</topic><topic>Tea</topic><topic>Theobromine</topic><topic>Theobromine - analysis</topic><topic>Theophylline</topic><topic>Theophylline - analysis</topic><topic>Water - chemistry</topic><topic>Xanthines - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tzanavaras, Paraskevas D.</creatorcontrib><creatorcontrib>Zacharis, Constantinos K.</creatorcontrib><creatorcontrib>Themelis, Demetrius G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tzanavaras, Paraskevas D.</au><au>Zacharis, Constantinos K.</au><au>Themelis, Demetrius G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid determination of methylxanthines in real samples by high-performance liquid chromatography using the new FastGradient ® narrow-bore monolithic column</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2010-06-15</date><risdate>2010</risdate><volume>81</volume><issue>4</issue><spage>1494</spage><epage>1501</epage><pages>1494-1501</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>The present study reports one of the very first analytical applications of the new narrow-bore monolithic column, FastGradient
® Chromolith (50
mm
×
2.0
mm i.d.). The three major methylxanthines (theobromine, theophylline and caffeine) were separated rapidly and determined simultaneously in various real samples. Based on the unique characteristics of this novel monolithic column the analytes were separated efficiently (
R
s
>
3.0) in less than 5
min at a low flow rate of 0.5
mL
min
−1 and using a low volume fraction of organic solvent (5% acetonitrile (ACN) in water) in the mobile phase. UV detection was carried out at 274
nm. The separation was optimized in terms of mobile phase composition, flow rate and injection volume, while the method was validated for linearity, detection and quantitation limits, within and day-to-day precision, accuracy and ruggedness. Its applicability was demonstrated by analyzing a variety of real samples including beverages, soft drinks, herbal products and pharmaceuticals. Compared to a well-established monolithic (Performance Chromolith 100
mm
×
4.6
mm i.d.) and a particulate reversed phase column (Hypersil ODS 5
μm 150
mm
×
4.6
mm i.d.), the narrow-bore FastGradient
® column offered satisfactory performance, faster analysis time and drastic reduction in the consumption of mobile phase and organic solvents.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20441929</pmid><doi>10.1016/j.talanta.2010.02.058</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0039-9140 |
| ispartof | Talanta (Oxford), 2010-06, Vol.81 (4), p.1494-1501 |
| issn | 0039-9140 1873-3573 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733948685 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Analytical chemistry Beverages - analysis Caffeine Caffeine - analysis Carbonated Beverages - analysis Chemistry Chemistry Techniques, Analytical Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Coffee Exact sciences and technology FastGradient ® monolithic column Food Analysis - methods Food Contamination High-performance liquid chromatography Limit of Detection Other chromatographic methods Pharmaceutical Preparations - analysis Reproducibility of Results Tablets Tea Theobromine Theobromine - analysis Theophylline Theophylline - analysis Water - chemistry Xanthines - analysis |
| title | Rapid determination of methylxanthines in real samples by high-performance liquid chromatography using the new FastGradient ® narrow-bore monolithic column |
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